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fluorogenic dpp4 assay kit  (BPS Bioscience)


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    Structured Review

    BPS Bioscience fluorogenic dpp4 assay kit
    Fluorogenic Dpp4 Assay Kit, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorogenic+dpp4+assay+kit/DPP4+Assay+Kit/10__1002_slash_slct__202505084-221-19-23
    Average 93 stars, based on 6 article reviews
    fluorogenic dpp4 assay kit - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    In Vitro:

    Article Title: Design, synthesis and evaluation the bioactivities of novel 8‑methoxy-1-azacoumarin-3-carboxamide derivatives as anti-diabetic agents
    Article Snippet: In this study, a novel series of 8‐methoxy-2-oxo-1,2-dihydroquinoline-3-carboxylic acid and N-substituted 8‐methoxy-2-oxo-1,2-dihydroquinoline-3-carboxamide derivatives 2–10 were designed, synthesized, and screened for their in vitro α-amylase, α-glucosidase and DPP-4 enzymes resulting in effective anti-diabetic agents.. The research results showed that these compounds had significant inhibitory effects on both α-amylase and α-glucosidase enzymes.. Specifically, compound 4 demonstrated the most potency with IC50 of 30.50±2.67 and 19.98±3.24μM for α-glucosidase and α-amylase activities respectively, which was significantly more powerful than the drug Acarbose (IC50 = 48.97±3.42μM and 16.59±3.78μM).

    Article Title: Novel Imidazole–Chromen‐2‐One Derivatives: Design, Synthesis, and Biological Evaluation as Potential Antidiabetic and Antioxidant Agents
    Article Snippet: A series of novel imidazole–chromen-2one derivatives (6–11) were designed, synthesized, and evaluated using an analogbased drug design approach for their potential dual α-glucosidase/ α-amylase inhibitory and anti-inflammatory activities.. In vitro biochemical assays were conducted to assess enzyme inhibition and antioxidant potential.. Among all tested compounds, compound (11) exhibited the most potent α-glucosidase and α-amylase inhibitory effects, with IC50 values comparable to the standard drug Acarbose , indicating significant antidiabetic potential.

    Recombinant:

    Article Title: Design, synthesis and evaluation the bioactivities of novel 8‑methoxy-1-azacoumarin-3-carboxamide derivatives as anti-diabetic agents
    Article Snippet: In this study, a novel series of 8‐methoxy-2-oxo-1,2-dihydroquinoline-3-carboxylic acid and N-substituted 8‐methoxy-2-oxo-1,2-dihydroquinoline-3-carboxamide derivatives 2–10 were designed, synthesized, and screened for their in vitro α-amylase, α-glucosidase and DPP-4 enzymes resulting in effective anti-diabetic agents.. The research results showed that these compounds had significant inhibitory effects on both α-amylase and α-glucosidase enzymes.. Specifically, compound 4 demonstrated the most potency with IC50 of 30.50±2.67 and 19.98±3.24μM for α-glucosidase and α-amylase activities respectively, which was significantly more powerful than the drug Acarbose (IC50 = 48.97±3.42μM and 16.59±3.78μM).

    Article Title: Novel Imidazole–Chromen‐2‐One Derivatives: Design, Synthesis, and Biological Evaluation as Potential Antidiabetic and Antioxidant Agents
    Article Snippet: A series of novel imidazole–chromen-2one derivatives (6–11) were designed, synthesized, and evaluated using an analogbased drug design approach for their potential dual α-glucosidase/ α-amylase inhibitory and anti-inflammatory activities.. In vitro biochemical assays were conducted to assess enzyme inhibition and antioxidant potential.. Among all tested compounds, compound (11) exhibited the most potent α-glucosidase and α-amylase inhibitory effects, with IC50 values comparable to the standard drug Acarbose , indicating significant antidiabetic potential.

    Enzyme Inhibition Assay:

    Article Title: Design, synthesis and evaluation the bioactivities of novel 8‑methoxy-1-azacoumarin-3-carboxamide derivatives as anti-diabetic agents
    Article Snippet: In this study, a novel series of 8‐methoxy-2-oxo-1,2-dihydroquinoline-3-carboxylic acid and N-substituted 8‐methoxy-2-oxo-1,2-dihydroquinoline-3-carboxamide derivatives 2–10 were designed, synthesized, and screened for their in vitro α-amylase, α-glucosidase and DPP-4 enzymes resulting in effective anti-diabetic agents.. The research results showed that these compounds had significant inhibitory effects on both α-amylase and α-glucosidase enzymes.. Specifically, compound 4 demonstrated the most potency with IC50 of 30.50±2.67 and 19.98±3.24μM for α-glucosidase and α-amylase activities respectively, which was significantly more powerful than the drug Acarbose (IC50 = 48.97±3.42μM and 16.59±3.78μM).



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    Schematic representation for <t>DPP-4/Substrate</t> complex.
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    Boxplot for the comparison between pre- and post-surgical values of serum <t>DPP4</t> activity. * p≤0.005; °outlier
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    Image Search Results


    Schematic representation for DPP-4/Substrate complex.

    Journal: Bioorganic Chemistry

    Article Title: Novel hit of DPP-4Is as promising antihyperglycemic agents with dual antioxidant/anti-inflammatory effects for type 2 diabetes with/without COVID-19

    doi: 10.1016/j.bioorg.2022.106092

    Figure Lengend Snippet: Schematic representation for DPP-4/Substrate complex.

    Article Snippet: The active compounds in SLM were screened for their DPP-4 inhibition activities as previously described , using Fluorogenic DPP-4 Assay Kit (Bps Bioscience Cornerstone Court W, Ste B San Diego, CA 92121) following the manufacturer’s instructions.

    Techniques:

    Naturally occurring phenolic compounds having DPP-4 inhibition potent activity.

    Journal: Bioorganic Chemistry

    Article Title: Novel hit of DPP-4Is as promising antihyperglycemic agents with dual antioxidant/anti-inflammatory effects for type 2 diabetes with/without COVID-19

    doi: 10.1016/j.bioorg.2022.106092

    Figure Lengend Snippet: Naturally occurring phenolic compounds having DPP-4 inhibition potent activity.

    Article Snippet: The active compounds in SLM were screened for their DPP-4 inhibition activities as previously described , using Fluorogenic DPP-4 Assay Kit (Bps Bioscience Cornerstone Court W, Ste B San Diego, CA 92121) following the manufacturer’s instructions.

    Techniques: Inhibition, Activity Assay

    In vivo DPP-4 activity as % of control in type2 diabetic rats, Values are mean ± SD (n = 3). ***significant (P < 0.001) compared to control diabetic group.

    Journal: Bioorganic Chemistry

    Article Title: Novel hit of DPP-4Is as promising antihyperglycemic agents with dual antioxidant/anti-inflammatory effects for type 2 diabetes with/without COVID-19

    doi: 10.1016/j.bioorg.2022.106092

    Figure Lengend Snippet: In vivo DPP-4 activity as % of control in type2 diabetic rats, Values are mean ± SD (n = 3). ***significant (P < 0.001) compared to control diabetic group.

    Article Snippet: The active compounds in SLM were screened for their DPP-4 inhibition activities as previously described , using Fluorogenic DPP-4 Assay Kit (Bps Bioscience Cornerstone Court W, Ste B San Diego, CA 92121) following the manufacturer’s instructions.

    Techniques: In Vivo, Activity Assay

    2D binding modes of compounds (4b, 4c, 6a, 6b, 7d, 8b) and Linagliptin with DPP-4 active site.

    Journal: Bioorganic Chemistry

    Article Title: Novel hit of DPP-4Is as promising antihyperglycemic agents with dual antioxidant/anti-inflammatory effects for type 2 diabetes with/without COVID-19

    doi: 10.1016/j.bioorg.2022.106092

    Figure Lengend Snippet: 2D binding modes of compounds (4b, 4c, 6a, 6b, 7d, 8b) and Linagliptin with DPP-4 active site.

    Article Snippet: The active compounds in SLM were screened for their DPP-4 inhibition activities as previously described , using Fluorogenic DPP-4 Assay Kit (Bps Bioscience Cornerstone Court W, Ste B San Diego, CA 92121) following the manufacturer’s instructions.

    Techniques: Binding Assay

    3D binding mode of compound 6a with DPP-4 active site.

    Journal: Bioorganic Chemistry

    Article Title: Novel hit of DPP-4Is as promising antihyperglycemic agents with dual antioxidant/anti-inflammatory effects for type 2 diabetes with/without COVID-19

    doi: 10.1016/j.bioorg.2022.106092

    Figure Lengend Snippet: 3D binding mode of compound 6a with DPP-4 active site.

    Article Snippet: The active compounds in SLM were screened for their DPP-4 inhibition activities as previously described , using Fluorogenic DPP-4 Assay Kit (Bps Bioscience Cornerstone Court W, Ste B San Diego, CA 92121) following the manufacturer’s instructions.

    Techniques: Binding Assay

    Boxplot for the comparison between pre- and post-surgical values of serum DPP4 activity. * p≤0.005; °outlier

    Journal: Obesity Surgery

    Article Title: Decrease of dipeptidyl peptidase 4 activity is associated with weight loss after bariatric surgery

    doi: 10.1007/s11695-020-05200-0

    Figure Lengend Snippet: Boxplot for the comparison between pre- and post-surgical values of serum DPP4 activity. * p≤0.005; °outlier

    Article Snippet: Serum DPP4 activity was assayed by a commercially available kit measuring the fluorescence of the cleaved fluorogenic DPP4 substrate H-Gly-Pro-AMC using a microplate reader (Enzo Life Sciences, Inc., Lausen, Switzerland).

    Techniques: Activity Assay