fluorescent protein mruby (Addgene inc)
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fluorescent protein mruby
Fluorescent Protein Mruby, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+protein+mruby/mRuby-N1+(Plasmid+%2354581)/pm41055982-267-17-23
Average 92 stars, based on 8 article reviews
Fluorescent Protein Mruby, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+protein+mruby/mRuby-N1+(Plasmid+%2354581)/pm41055982-267-17-23
Average 92 stars, based on 8 article reviews
fluorescent protein mruby - by Bioz Stars,
2026-10
92/100 stars
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Construct:Article Title: STAR/STARD1: a mitochondrial intermembrane space cholesterol shuttle degraded through mitophagy Article Snippet: Fusion of fluorescent proteins were also constructed in the pMX retroviral vector, for which a C-terminal Emerald (from Emerald-N1; Addgene plasmid 54588) was inserted into pMX-ΔN36-STAR and pMX-ΔN62-STAR. .. Fluorescent proteins targeting the OMM was constructed by fusion of TOM20 (amplified from murine testis cDNA) and Article Title: STAR/STARD1: A mitochondrial intermembrane space cholesterol shuttle degraded through mitophagy. Article Snippet: Fusion of fluorescent proteins was also constructed in the pMX retroviral vector, for which a C- terminal Emerald (from Emerald- N1; Addgene plasmid 54588) was inserted into pMX- ∆N36- STAR and pMX- ∆N62- STAR. .. Fluorescent proteins targeting the OMM were constructed by fusion of TOM20 (amplified from murine testis cDNA) and Amplification:Article Title: STAR/STARD1: a mitochondrial intermembrane space cholesterol shuttle degraded through mitophagy Article Snippet: Fusion of fluorescent proteins were also constructed in the pMX retroviral vector, for which a C-terminal Emerald (from Emerald-N1; Addgene plasmid 54588) was inserted into pMX-ΔN36-STAR and pMX-ΔN62-STAR. .. Fluorescent proteins targeting the OMM was constructed by fusion of TOM20 (amplified from murine testis cDNA) and Article Title: STAR/STARD1: A mitochondrial intermembrane space cholesterol shuttle degraded through mitophagy. Article Snippet: Fusion of fluorescent proteins was also constructed in the pMX retroviral vector, for which a C- terminal Emerald (from Emerald- N1; Addgene plasmid 54588) was inserted into pMX- ∆N36- STAR and pMX- ∆N62- STAR. .. Fluorescent proteins targeting the OMM were constructed by fusion of TOM20 (amplified from murine testis cDNA) and Plasmid Preparation:Article Title: STAR/STARD1: a mitochondrial intermembrane space cholesterol shuttle degraded through mitophagy Article Snippet: Fusion of fluorescent proteins were also constructed in the pMX retroviral vector, for which a C-terminal Emerald (from Emerald-N1; Addgene plasmid 54588) was inserted into pMX-ΔN36-STAR and pMX-ΔN62-STAR. .. Fluorescent proteins targeting the OMM was constructed by fusion of TOM20 (amplified from murine testis cDNA) and Article Title: Mammalian cell line for protein production and library generation Article Snippet: .. The PnP cell line consists of the following components: 1) The endogenous VH gene in the IgH locus was replaced with a Article Title: STAR/STARD1: A mitochondrial intermembrane space cholesterol shuttle degraded through mitophagy. Article Snippet: Fusion of fluorescent proteins was also constructed in the pMX retroviral vector, for which a C- terminal Emerald (from Emerald- N1; Addgene plasmid 54588) was inserted into pMX- ∆N36- STAR and pMX- ∆N62- STAR. .. Fluorescent proteins targeting the OMM were constructed by fusion of TOM20 (amplified from murine testis cDNA) and Retroviral:Article Title: STAR/STARD1: a mitochondrial intermembrane space cholesterol shuttle degraded through mitophagy Article Snippet: Fusion of fluorescent proteins were also constructed in the pMX retroviral vector, for which a C-terminal Emerald (from Emerald-N1; Addgene plasmid 54588) was inserted into pMX-ΔN36-STAR and pMX-ΔN62-STAR. .. Fluorescent proteins targeting the OMM was constructed by fusion of TOM20 (amplified from murine testis cDNA) and Article Title: STAR/STARD1: A mitochondrial intermembrane space cholesterol shuttle degraded through mitophagy. Article Snippet: Fusion of fluorescent proteins was also constructed in the pMX retroviral vector, for which a C- terminal Emerald (from Emerald- N1; Addgene plasmid 54588) was inserted into pMX- ∆N36- STAR and pMX- ∆N62- STAR. .. Fluorescent proteins targeting the OMM were constructed by fusion of TOM20 (amplified from murine testis cDNA) and |
![MSGNs and their connections across the hippocampus remain despite hippocampal sclerosis. ( A ) Top : Schematic of viral expression and cannula placement. Cre-dependent <t>GFP</t> and mRuby conjugated to synaptophsyin were expressed in MSGNs by AAV injection into medial septum of VGAT::cre mice. A cannula was implanted for kainate or vehicle (saline) unilateral injection over rostral-dorsal hippocampus. Numbers correspond to approximate hippocampal rostral-caudal levels imaged for puncta analysis. Bottom : Experimental timeline. ( B ) Representative GFP-mRuby AAV expression in MSGNs and staining for Neurotrace and GABA in saline and kainate treated mice. Scale bar = 50 µm. Examples of neurons expressing GFP, mRuby or GABA (arrows) and neurons not expressing GFP, mRuby or GABA (arrowheads). We found 96.16 ± 6.46% of mRuby-GFP expressing cells co-expressed GABA. ( C ) Neuronal populations in saline and kainate treated mice. Horizontal lines indicate mean values [mean ± standard error of the mean (SEM)]. Points correspond to values from individual mice. Populations were not significantly decreased in kainate-treated mice when compared to saline-treated controls (two-way ANOVA, P = 0.58, F = 0.31, df = 1, n of saline and kainate treated mice per cell type, respectively: mRuby-GFP labelled cells in VGAT::Cre mice n = 6, 7; GABA n = 3, 5; PV, CB, ChAT n = 4, 4). ( D ) Representative hippocampal sections of rostral-caudal levels stained with <t>fluorescent</t> Neurotrace are shown in a saline-injected mouse, and the contralateral and ipsilateral hippocampi of a kainate-injected mouse. Scale bars = 200 µm. Expression of GFP (green) in MSGN axons across the hippocampus and sclerosis in rostral slices ipsilateral to kainate injection. ( E ) Putative synaptic terminals expressing mRuby in the hilus at second rostral-caudal level contralateral and ipsilateral to kainate injection (dashed white boxes in D ). Scale bar = 10 µm. ( F ) Density of synaptic terminals across rostral-caudal levels in the hilus and the entire hippocampus in saline-treated mice and contralateral and ipsilateral hippocampi of kainate-treated mice. Bars indicate mean (mean ± SEM). Points correspond to values from individual mice. Synaptic density did not decrease in kainate-treated mice (two-way ANOVA, P = 0.57, F = 0.87, df = 11, n = 5 mice per treatment). Puncta counts were reported normalized to the number of virus-labelled cells in medial septum.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_9369/pmc08219369/pmc08219369__awab042f1.jpg)