fluorescent probe dhe kit  (Keygen Biotech)

Journal: Bioengineering

Article Title: Fe 3 O 4 Magnetic Nanoparticles and Static Magnetic Field Stimulated BMSC-Derived Exosomes Promoted Osteogenesis and Alleviated Oxidative Stress in Irradiated BMSCs Through miR-429/NOG Pathway

doi: 10.3390/bioengineering13040402

Figure Lengend Snippet: BMSC-Fe 3 O 4 -SMF-Exos Mitigate Intracellular ROS in Irradiated BMSCs. ( a , c ) Intracellular total ROS levels in BMSCs were evaluated utilizing the 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA) fluorescent probe, accompanied by the relevant quantitative determination. ( b , d ) Superoxide anion (O 2 − ) production in irradiated BMSCs was monitored with the DHE fluorescent probe, and concurrent quantitative analysis was implemented. ( e ) The concentration of MDA in irradiated BMSCs was quantified. ( f , g ) The levels of SOD in irradiated BMSCs were measured at the 24 h and 48 h time points, separately. n = 3; (**) p < 0.01; (***) p < 0.001, and (****) p < 0.0001.

Article Snippet: Similarly, intracellular superoxide anion levels were measured using a Dihydroethidium (DHE) Superoxide Anion Fluorescent Probe Kit (G1904-100T, Servicebio, Wuhan, Hubei, China) in full accordance with the manufacturer’s instructions.

Techniques: Irradiation, Concentration Assay

Journal: Materials Today Bio

Article Title: A multifunctional parathyroid hormone-related supramolecular peptide-loaded dual network hydrogel for radiation-induced wound repair

doi: 10.1016/j.mtbio.2025.101807

Figure Lengend Snippet: Antioxidative strategies, mitochondrial safeguarding, and anti-aging interventions of QOP@P1R16 hydrogel. (A) Optical image of DPPH scavenging test on hydrogels. (B) DPPH scavenging rate of hydrogels. (C–D) ROS flow cytometry with different hydrogel and quantitative analysis results. (E) DCFH-DA probe experiment, (F) mitochondrial membrane potential detection kit, (G) β-galactosidase staining experiment, (H) γ -H2AX immunofluorescence staining and (I) p21immunofluorescence staining on different groups. (J–K) Quantitative analysis results of (E–I). The data are presented as the mean ± SD (n = 3). ∗(p < 0.05), ∗∗(p < 0.01), ∗∗∗(p < 0.001) and ∗∗∗∗ (p < 0.0001) indicate significant differences compared to PBS, &(p < 0.05),&&(p < 0.01), &&&(p < 0.001) and &&&&(p < 0.0001) compared to IR.

Article Snippet: The production of ROS in irradiated L929 cells and the protective effects of the hydrogel were measured using the ROS-detecting fluorescent probe dihydroethidium (DHE) assay kit (KeyGen, China).

Techniques: Flow Cytometry, Membrane, Staining, Immunofluorescence

Journal: Journal of Orthopaedic Translation

Article Title: Melatonin alleviates oxidative stress-induced injury to nucleus pulposus-derived mesenchymal stem cells through activating PI3K/Akt pathway

doi: 10.1016/j.jot.2023.10.002

Figure Lengend Snippet: Melatonin protects mitochondrial homeostasis by reducing mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) in nucleus pulposus-derived mesenchymal stem cells (NPMSCs). ( A ) Fluorescence results of ROS in the different groups. Red fluorescence represents a high level of ROS ( B ) Quantitative analysis of ROS fluorescence results. Quantitative analysis of MMP results. ( C ) Fluorescence results of MMP in the different groups. Red fluorescence represents the mitochondrial aggregate JC-1 and green fluorescence indicates the monomeric JC-1 (White scale bar = 100 μm; red scale bar = 25 μm). ( D ) Quantitative analysis of MMP results. All data are the mean ± SD. **: P < 0.01 compared with the control group; ## : P < 0.01 compared with the H 2 O 2 group; && : P < 0.01 compared with the Melatonin group. PI3K-IN-1: PI3K-IN-1 group.

Article Snippet: In order to verify the effect of melatonin on ROS production under oxidative stress, ROS fluorescence probe dihydroethidium (DHE) kit (KeyGen, China) was used to detect ROS production in NPMSCs.

Techniques: Membrane, Derivative Assay, Fluorescence, Control