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fluorescent avidin biotin complex kit  (Vector Laboratories)


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    Structured Review

    Vector Laboratories fluorescent avidin biotin complex kit
    Fluorescent Avidin Biotin Complex Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 18030 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescent+avidin/VECTASTAIN+ABC+HRP+Kit+(Peroxidase%2C+Standard)/pm41187812-343-4-11
    Average 96 stars, based on 18030 article reviews
    fluorescent avidin biotin complex kit - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Staining:

    Article Title: Noise-Induced Dysregulation of Quaking RNA Binding Proteins Contributes to Auditory Nerve Demyelination and Hearing Loss
    Article Snippet: .. Staining was performed either indirectly using biotinylated secondaries conjugated with fluorescent avidin (Vector Laboratories) or directly using Alexa Fluor Dyes (Thermo Fisher Scientific). ..

    Article Title: Peripheral Auditory Nerve Impairment in a Mouse Model of Syndromic Autism
    Article Snippet: .. Staining was performed by either indirect method using biotinylated secondary antibodies conjugated with fluorescent avidin (Vector Labs) or direct method using primary antibodies conjugated to AlexaFluor dyes (Thermo Fisher Scientific). ..

    Article Title: Two distinct types of nodes of Ranvier support auditory nerve function in the mouse cochlea
    Article Snippet: .. Staining was performed by either indirect method using biotinylated secondaries conjugated with fluorescent avidin (Vector Labs, Burlingame, CA) or direct method using Alexa Fluor Dyes (ThermoFisher Scientific, Waltham, MA). ..

    Article Title: Noise-Induced Dysregulation of Quaking RNA Binding Proteins Contributes to Auditory Nerve Demyelination and Hearing Loss
    Article Snippet: .. Staining was performed either indirectly 268 using biotinylated secondaries conjugated with fluorescent avidin (Vector Labs, Burlingame, CA) or directly 269 using Alexa Fluor Dyes (ThermoFisher Scientific, Waltham, MA). ..

    Article Title: Peripheral Auditory Nerve Impairment in a Mouse Model of Syndromic Autism
    Article Snippet: .. Staining was performed by either indirect method using biotinylated secondary antibodies conjugated with fluorescent avidin (Vector Labs) or direct method using primary antibodies conjugated to AlexaFluor dyes (Thermo Fisher Scientific). ..

    Avidin-Biotin Assay:

    Article Title: Noise-Induced Dysregulation of Quaking RNA Binding Proteins Contributes to Auditory Nerve Demyelination and Hearing Loss
    Article Snippet: .. Staining was performed either indirectly using biotinylated secondaries conjugated with fluorescent avidin (Vector Laboratories) or directly using Alexa Fluor Dyes (Thermo Fisher Scientific). ..

    Article Title: Peripheral Auditory Nerve Impairment in a Mouse Model of Syndromic Autism
    Article Snippet: .. Staining was performed by either indirect method using biotinylated secondary antibodies conjugated with fluorescent avidin (Vector Labs) or direct method using primary antibodies conjugated to AlexaFluor dyes (Thermo Fisher Scientific). ..

    Article Title: Two distinct types of nodes of Ranvier support auditory nerve function in the mouse cochlea
    Article Snippet: .. Staining was performed by either indirect method using biotinylated secondaries conjugated with fluorescent avidin (Vector Labs, Burlingame, CA) or direct method using Alexa Fluor Dyes (ThermoFisher Scientific, Waltham, MA). ..

    Article Title: Elevated Peritoneal Fluid TNF-? Incites Ovarian Early Growth Response Factor 1 Expression and Downstream Protease Mediators
    Article Snippet: .. A rabbit anti-EGR1 monoclonal antibody (1:1000; 0.035 ug/mL; Cell Signaling Technology, Danvers, Massachusetts, cat no. 4153), biotinylated secondary antibody (Vector Laboratories, cat no. BA-1000), fluorescent avidin (FITC, Vector Laboratories, cat no. A-2011), and 4′,6-diamidino-2-phenylindole as routinely performed by our laboratory. ..

    Article Title: Competition between the Brain and Testes under Selenium-Compromised Conditions: Insight into Sex Differences in Selenium Metabolism and Risk of Neurodevelopmental Disease
    Article Snippet: .. To label perineuronal nets (PNNs), sections were incubated in a solution containing biotinylated Wisteria floribunda lectin (1:1000; Sigma, L1516), washed, and then incubated with fluorescent avidin (1:500; Vector Labs) for visualization. ..

    Article Title: Competition between the Brain and Testes under Selenium-Compromised Conditions: Insight into Sex Differences in Selenium Metabolism and Risk of Neurodevelopmental Disease
    Article Snippet: .. To label perineuronal nets (PNNs), sections were incubated in a solution containing biotinylated Wisteria floribunda lectin (1:1000; Sigma, L1516), washed, and then incubated with fluorescent avidin (1:500; Vector Labs) for visualization. ..

    Article Title: Noise-Induced Dysregulation of Quaking RNA Binding Proteins Contributes to Auditory Nerve Demyelination and Hearing Loss
    Article Snippet: .. Staining was performed either indirectly 268 using biotinylated secondaries conjugated with fluorescent avidin (Vector Labs, Burlingame, CA) or directly 269 using Alexa Fluor Dyes (ThermoFisher Scientific, Waltham, MA). ..

    Article Title: Peripheral Auditory Nerve Impairment in a Mouse Model of Syndromic Autism
    Article Snippet: .. Staining was performed by either indirect method using biotinylated secondary antibodies conjugated with fluorescent avidin (Vector Labs) or direct method using primary antibodies conjugated to AlexaFluor dyes (Thermo Fisher Scientific). ..

    Incubation:

    Article Title: Competition between the Brain and Testes under Selenium-Compromised Conditions: Insight into Sex Differences in Selenium Metabolism and Risk of Neurodevelopmental Disease
    Article Snippet: .. To label perineuronal nets (PNNs), sections were incubated in a solution containing biotinylated Wisteria floribunda lectin (1:1000; Sigma, L1516), washed, and then incubated with fluorescent avidin (1:500; Vector Labs) for visualization. ..

    Article Title: Competition between the Brain and Testes under Selenium-Compromised Conditions: Insight into Sex Differences in Selenium Metabolism and Risk of Neurodevelopmental Disease
    Article Snippet: .. To label perineuronal nets (PNNs), sections were incubated in a solution containing biotinylated Wisteria floribunda lectin (1:1000; Sigma, L1516), washed, and then incubated with fluorescent avidin (1:500; Vector Labs) for visualization. ..



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    Chr4 Δ70/Δ70 does not affect pancreatic β-cell number but may reduce the cell proliferation rate already in young Ldlr −/− ApoB 100/100 mice. ( A ) Pancreatic islet size, ( B ) proportion of α- and β-cells, and ( C ) proliferation of mixed and ( D ) >10,000 μm 2 pancreatic islets in young Ldlr −/− ApoB 100/100 ( n = 5–7) and Chr4 Δ70/Δ70 mice ( n = 5–7). Primary antibody against glucagon was used as a marker for the pancreatic α-cells, and insulin ab was used for the β-cells. For visualization, <t>fluorescent</t> secondary antibodies were used. In the representative figures, glucagon positive cells appear in red and insulin in green. Nuclei were counterstained with DAPI (blue). Ki-67 antibody was used as a marker of cell proliferation and visualized with DAB. ( E ) For the islet function, insulin secretion of Ldlr −/− ApoB 100/100 ( n = 4) and Chr4 Δ70/Δ70 mice ( n = 4) was measured both in fasted state (0 min) and in response to 1 g/kg i.p. glucose at time points 15, 30, 60 and 90 min after the administration. Asterisk (*) indicates statistical significance. Difference in mean between Ldlr −/− ApoB 100/100 and Chr4 Δ70/Δ70 mice was measured by using t -test, and it was considered statistically significant when p < 0.05.
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    Chr4 Δ70/Δ70 does not affect pancreatic β-cell number but may reduce the cell proliferation rate already in young Ldlr −/− ApoB 100/100 mice. ( A ) Pancreatic islet size, ( B ) proportion of α- and β-cells, and ( C ) proliferation of mixed and ( D ) >10,000 μm 2 pancreatic islets in young Ldlr −/− ApoB 100/100 ( n = 5–7) and Chr4 Δ70/Δ70 mice ( n = 5–7). Primary antibody against glucagon was used as a marker for the pancreatic α-cells, and insulin ab was used for the β-cells. For visualization, <t>fluorescent</t> secondary antibodies were used. In the representative figures, glucagon positive cells appear in red and insulin in green. Nuclei were counterstained with DAPI (blue). Ki-67 antibody was used as a marker of cell proliferation and visualized with DAB. ( E ) For the islet function, insulin secretion of Ldlr −/− ApoB 100/100 ( n = 4) and Chr4 Δ70/Δ70 mice ( n = 4) was measured both in fasted state (0 min) and in response to 1 g/kg i.p. glucose at time points 15, 30, 60 and 90 min after the administration. Asterisk (*) indicates statistical significance. Difference in mean between Ldlr −/− ApoB 100/100 and Chr4 Δ70/Δ70 mice was measured by using t -test, and it was considered statistically significant when p < 0.05.
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    Image Search Results


    Chr4 Δ70/Δ70 does not affect pancreatic β-cell number but may reduce the cell proliferation rate already in young Ldlr −/− ApoB 100/100 mice. ( A ) Pancreatic islet size, ( B ) proportion of α- and β-cells, and ( C ) proliferation of mixed and ( D ) >10,000 μm 2 pancreatic islets in young Ldlr −/− ApoB 100/100 ( n = 5–7) and Chr4 Δ70/Δ70 mice ( n = 5–7). Primary antibody against glucagon was used as a marker for the pancreatic α-cells, and insulin ab was used for the β-cells. For visualization, fluorescent secondary antibodies were used. In the representative figures, glucagon positive cells appear in red and insulin in green. Nuclei were counterstained with DAPI (blue). Ki-67 antibody was used as a marker of cell proliferation and visualized with DAB. ( E ) For the islet function, insulin secretion of Ldlr −/− ApoB 100/100 ( n = 4) and Chr4 Δ70/Δ70 mice ( n = 4) was measured both in fasted state (0 min) and in response to 1 g/kg i.p. glucose at time points 15, 30, 60 and 90 min after the administration. Asterisk (*) indicates statistical significance. Difference in mean between Ldlr −/− ApoB 100/100 and Chr4 Δ70/Δ70 mice was measured by using t -test, and it was considered statistically significant when p < 0.05.

    Journal: Cells

    Article Title: Deletion of the Murine Ortholog of the Human 9p21.3 Locus Leads to Insulin Resistance and Obesity in Hypercholesterolemic Mice

    doi: 10.3390/cells13110983

    Figure Lengend Snippet: Chr4 Δ70/Δ70 does not affect pancreatic β-cell number but may reduce the cell proliferation rate already in young Ldlr −/− ApoB 100/100 mice. ( A ) Pancreatic islet size, ( B ) proportion of α- and β-cells, and ( C ) proliferation of mixed and ( D ) >10,000 μm 2 pancreatic islets in young Ldlr −/− ApoB 100/100 ( n = 5–7) and Chr4 Δ70/Δ70 mice ( n = 5–7). Primary antibody against glucagon was used as a marker for the pancreatic α-cells, and insulin ab was used for the β-cells. For visualization, fluorescent secondary antibodies were used. In the representative figures, glucagon positive cells appear in red and insulin in green. Nuclei were counterstained with DAPI (blue). Ki-67 antibody was used as a marker of cell proliferation and visualized with DAB. ( E ) For the islet function, insulin secretion of Ldlr −/− ApoB 100/100 ( n = 4) and Chr4 Δ70/Δ70 mice ( n = 4) was measured both in fasted state (0 min) and in response to 1 g/kg i.p. glucose at time points 15, 30, 60 and 90 min after the administration. Asterisk (*) indicates statistical significance. Difference in mean between Ldlr −/− ApoB 100/100 and Chr4 Δ70/Δ70 mice was measured by using t -test, and it was considered statistically significant when p < 0.05.

    Article Snippet: Pancreatic α- and β-cells were immunostained with primary antibodies against glucagon (Dako A0565, Rabbit anti-human glucagon, Agilent, Santa Clara, CA, USA) and insulin (Dako A0564, Guinea pig anti-insulin, Agilent, Santa Clara, CA, USA) and appropriate fluorescent secondary antibodies (for glucagon, A21442, chicken anti-rabbit A594, Thermo Fisher Scientific, Waltham, MA, USA, and for insulin, BA-7000 Goat anti-guinea pig with A-2011 Fluorescein Avidin DCS, Vector laboratories, Newark, CA, USA).

    Techniques: Marker