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inverted fluorescence microscope  (Nikon)


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    Nikon inverted fluorescence microscope
    Inverted Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 11616 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescence+microscope+ti2/ECLIPSE+Ti2/pmc12934279-126-14-17
    Average 99 stars, based on 11616 article reviews
    inverted fluorescence microscope - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Staining:

    Article Title: Tetrahedral framework nucleic acids-based delivery of microRNA-155 alleviates intervertebral disc degeneration through targeting Bcl-2/Bax apoptosis pathway.
    Article Snippet: Finally, the samples were observed under a fluorescence microscope (Ti2, Nikon, Japan).

    Article Title: 3D bio scaffold support osteogenic differentiation of mesenchymal stem cells.
    Article Snippet: Funding information Higher Education Commission, Pakistan, Grant/Award Number: 7083 Abstract The regeneration of osteochondral lesions by tissue engineering techniques is challenging due to the lack of physicochemical characteristics and dual‐lineage (osteogenesis and chondrogenesis).. A scaffold with better mechanical properties and dual lineage capability is required for the regeneration of osteochondral defects.. In this study, a hydrogel prepared from decellularized human umbilical cord tissue was developed and evaluated for osteochondral regeneration.

    Article Title: Bone morphogenetic protein 15 gene disruption affects the in vitro maturation of porcine oocytes by impairing spindle assembly and organelle function.
    Article Snippet: Bone morphogenetic protein 15 (BMP15) plays a crucial role in the porcine follicular development.. However, its exact functions in the in vitro maturation (IVM) of porcine oocytes remain largely unknown.. Here, through cytoplasmic injection of a preassembled crRNA–tracrRNA–Cas9 ribonucleoprotein complex, we achieved BMP15 disruption in approximately 54 % of the cultured porcine oocytes.

    Article Title: Ru single-atom nanozymes targeting ROS-ferroptosis pathways for enhanced endometrial regeneration in intrauterine adhesion therapy.
    Article Snippet: In brief, cells were stained with DCFH-DA (10 μM) and incubated in a cell incubator at 37 ◦C for 20 min. After three washes with serum-free cell culture medium to eliminate residual DCFH-DA, the cells were examined using a fluorescence microscope (Ti2, Nikon, Japan), following the protocol described elsewhere [79].

    Article Title: A biomimetic human disease model of bacterial keratitis using a cornea-on-a-chip system.
    Article Snippet: Bacterial keratitis is a common form of inflammation caused by the bacterial invasion of the corneal stroma after trauma.. In extreme cases, it can lead to severe visual impairment or even blindness; therefore, timely medical intervention is imperative.. Unfortunately, widespread misuse of antibiotics has led to the development of drug resistance.

    Article Title: Multifunctional and immunoregulatory microenvironment-responsive hydrogel for whole course management of infected diabetic wounds
    Article Snippet: Cells on the underside of the microporous submembrane were visualized under a fluorescence microscope (Ti2, Nikon, Tokyo, Japan).

    Article Title: Docosahexaenoic acid supplementation during porcine oocyte in vitro maturation improves oocyte quality and embryonic development by enhancing the homeostasis of energy metabolism.
    Article Snippet: Although supplementation with docosahexaenoic acid (DHA) during porcine oocyte IVM is well-established, the available data are limited due to the lack of consistency.. Moreover, to our knowledge, the anti-oxidant effects of DHA on porcine oocytes have not been reported.. Hence, this study aimed to examine the effects of DHA supplementation on the regulation of energy metabolism during porcine oocyte maturation to improve oocyte maturation and embryonic development.

    Article Title: Multifunctional and immunoregulatory microenvironment-responsive hydrogel for whole course management of infected diabetic wounds
    Article Snippet: The cells were then observed under a fluorescence microscope (Ti2, Nikon, Tokyo, Japan).

    Incubation:

    Article Title: Tetrahedral framework nucleic acids-based delivery of microRNA-155 alleviates intervertebral disc degeneration through targeting Bcl-2/Bax apoptosis pathway.
    Article Snippet: Finally, the samples were observed under a fluorescence microscope (Ti2, Nikon, Japan).

    Article Title: 3D bio scaffold support osteogenic differentiation of mesenchymal stem cells.
    Article Snippet: Funding information Higher Education Commission, Pakistan, Grant/Award Number: 7083 Abstract The regeneration of osteochondral lesions by tissue engineering techniques is challenging due to the lack of physicochemical characteristics and dual‐lineage (osteogenesis and chondrogenesis).. A scaffold with better mechanical properties and dual lineage capability is required for the regeneration of osteochondral defects.. In this study, a hydrogel prepared from decellularized human umbilical cord tissue was developed and evaluated for osteochondral regeneration.

    Article Title: Bone morphogenetic protein 15 gene disruption affects the in vitro maturation of porcine oocytes by impairing spindle assembly and organelle function.
    Article Snippet: Bone morphogenetic protein 15 (BMP15) plays a crucial role in the porcine follicular development.. However, its exact functions in the in vitro maturation (IVM) of porcine oocytes remain largely unknown.. Here, through cytoplasmic injection of a preassembled crRNA–tracrRNA–Cas9 ribonucleoprotein complex, we achieved BMP15 disruption in approximately 54 % of the cultured porcine oocytes.

    Article Title: Ru single-atom nanozymes targeting ROS-ferroptosis pathways for enhanced endometrial regeneration in intrauterine adhesion therapy.
    Article Snippet: In brief, cells were stained with DCFH-DA (10 μM) and incubated in a cell incubator at 37 ◦C for 20 min. After three washes with serum-free cell culture medium to eliminate residual DCFH-DA, the cells were examined using a fluorescence microscope (Ti2, Nikon, Japan), following the protocol described elsewhere [79].

    Article Title: A biomimetic human disease model of bacterial keratitis using a cornea-on-a-chip system.
    Article Snippet: Bacterial keratitis is a common form of inflammation caused by the bacterial invasion of the corneal stroma after trauma.. In extreme cases, it can lead to severe visual impairment or even blindness; therefore, timely medical intervention is imperative.. Unfortunately, widespread misuse of antibiotics has led to the development of drug resistance.

    Article Title: Multifunctional and immunoregulatory microenvironment-responsive hydrogel for whole course management of infected diabetic wounds
    Article Snippet: Cells on the underside of the microporous submembrane were visualized under a fluorescence microscope (Ti2, Nikon, Tokyo, Japan).

    Article Title: Docosahexaenoic acid supplementation during porcine oocyte in vitro maturation improves oocyte quality and embryonic development by enhancing the homeostasis of energy metabolism.
    Article Snippet: Although supplementation with docosahexaenoic acid (DHA) during porcine oocyte IVM is well-established, the available data are limited due to the lack of consistency.. Moreover, to our knowledge, the anti-oxidant effects of DHA on porcine oocytes have not been reported.. Hence, this study aimed to examine the effects of DHA supplementation on the regulation of energy metabolism during porcine oocyte maturation to improve oocyte maturation and embryonic development.

    Article Title: Multifunctional and immunoregulatory microenvironment-responsive hydrogel for whole course management of infected diabetic wounds
    Article Snippet: The cells were then observed under a fluorescence microscope (Ti2, Nikon, Tokyo, Japan).

    Cell Culture:

    Article Title: Tetrahedral framework nucleic acids-based delivery of microRNA-155 alleviates intervertebral disc degeneration through targeting Bcl-2/Bax apoptosis pathway.
    Article Snippet: Finally, the samples were observed under a fluorescence microscope (Ti2, Nikon, Japan).

    Article Title: 3D bio scaffold support osteogenic differentiation of mesenchymal stem cells.
    Article Snippet: Funding information Higher Education Commission, Pakistan, Grant/Award Number: 7083 Abstract The regeneration of osteochondral lesions by tissue engineering techniques is challenging due to the lack of physicochemical characteristics and dual‐lineage (osteogenesis and chondrogenesis).. A scaffold with better mechanical properties and dual lineage capability is required for the regeneration of osteochondral defects.. In this study, a hydrogel prepared from decellularized human umbilical cord tissue was developed and evaluated for osteochondral regeneration.

    Article Title: Bone morphogenetic protein 15 gene disruption affects the in vitro maturation of porcine oocytes by impairing spindle assembly and organelle function.
    Article Snippet: Bone morphogenetic protein 15 (BMP15) plays a crucial role in the porcine follicular development.. However, its exact functions in the in vitro maturation (IVM) of porcine oocytes remain largely unknown.. Here, through cytoplasmic injection of a preassembled crRNA–tracrRNA–Cas9 ribonucleoprotein complex, we achieved BMP15 disruption in approximately 54 % of the cultured porcine oocytes.

    Article Title: Ru single-atom nanozymes targeting ROS-ferroptosis pathways for enhanced endometrial regeneration in intrauterine adhesion therapy.
    Article Snippet: In brief, cells were stained with DCFH-DA (10 μM) and incubated in a cell incubator at 37 ◦C for 20 min. After three washes with serum-free cell culture medium to eliminate residual DCFH-DA, the cells were examined using a fluorescence microscope (Ti2, Nikon, Japan), following the protocol described elsewhere [79].

    Article Title: A biomimetic human disease model of bacterial keratitis using a cornea-on-a-chip system.
    Article Snippet: Bacterial keratitis is a common form of inflammation caused by the bacterial invasion of the corneal stroma after trauma.. In extreme cases, it can lead to severe visual impairment or even blindness; therefore, timely medical intervention is imperative.. Unfortunately, widespread misuse of antibiotics has led to the development of drug resistance.

    Article Title: Multifunctional and immunoregulatory microenvironment-responsive hydrogel for whole course management of infected diabetic wounds
    Article Snippet: Cells on the underside of the microporous submembrane were visualized under a fluorescence microscope (Ti2, Nikon, Tokyo, Japan).

    Article Title: Docosahexaenoic acid supplementation during porcine oocyte in vitro maturation improves oocyte quality and embryonic development by enhancing the homeostasis of energy metabolism.
    Article Snippet: Although supplementation with docosahexaenoic acid (DHA) during porcine oocyte IVM is well-established, the available data are limited due to the lack of consistency.. Moreover, to our knowledge, the anti-oxidant effects of DHA on porcine oocytes have not been reported.. Hence, this study aimed to examine the effects of DHA supplementation on the regulation of energy metabolism during porcine oocyte maturation to improve oocyte maturation and embryonic development.

    Article Title: Multifunctional and immunoregulatory microenvironment-responsive hydrogel for whole course management of infected diabetic wounds
    Article Snippet: The cells were then observed under a fluorescence microscope (Ti2, Nikon, Tokyo, Japan).

    Fluorescence:

    Article Title: Tetrahedral framework nucleic acids-based delivery of microRNA-155 alleviates intervertebral disc degeneration through targeting Bcl-2/Bax apoptosis pathway.
    Article Snippet: Finally, the samples were observed under a fluorescence microscope (Ti2, Nikon, Japan).

    Article Title: 3D bio scaffold support osteogenic differentiation of mesenchymal stem cells.
    Article Snippet: Funding information Higher Education Commission, Pakistan, Grant/Award Number: 7083 Abstract The regeneration of osteochondral lesions by tissue engineering techniques is challenging due to the lack of physicochemical characteristics and dual‐lineage (osteogenesis and chondrogenesis).. A scaffold with better mechanical properties and dual lineage capability is required for the regeneration of osteochondral defects.. In this study, a hydrogel prepared from decellularized human umbilical cord tissue was developed and evaluated for osteochondral regeneration.

    Article Title: Bone morphogenetic protein 15 gene disruption affects the in vitro maturation of porcine oocytes by impairing spindle assembly and organelle function.
    Article Snippet: Bone morphogenetic protein 15 (BMP15) plays a crucial role in the porcine follicular development.. However, its exact functions in the in vitro maturation (IVM) of porcine oocytes remain largely unknown.. Here, through cytoplasmic injection of a preassembled crRNA–tracrRNA–Cas9 ribonucleoprotein complex, we achieved BMP15 disruption in approximately 54 % of the cultured porcine oocytes.

    Article Title: Ru single-atom nanozymes targeting ROS-ferroptosis pathways for enhanced endometrial regeneration in intrauterine adhesion therapy.
    Article Snippet: In brief, cells were stained with DCFH-DA (10 μM) and incubated in a cell incubator at 37 ◦C for 20 min. After three washes with serum-free cell culture medium to eliminate residual DCFH-DA, the cells were examined using a fluorescence microscope (Ti2, Nikon, Japan), following the protocol described elsewhere [79].

    Article Title: A biomimetic human disease model of bacterial keratitis using a cornea-on-a-chip system.
    Article Snippet: Bacterial keratitis is a common form of inflammation caused by the bacterial invasion of the corneal stroma after trauma.. In extreme cases, it can lead to severe visual impairment or even blindness; therefore, timely medical intervention is imperative.. Unfortunately, widespread misuse of antibiotics has led to the development of drug resistance.

    Article Title: Multifunctional and immunoregulatory microenvironment-responsive hydrogel for whole course management of infected diabetic wounds
    Article Snippet: Cells on the underside of the microporous submembrane were visualized under a fluorescence microscope (Ti2, Nikon, Tokyo, Japan).

    Article Title: Docosahexaenoic acid supplementation during porcine oocyte in vitro maturation improves oocyte quality and embryonic development by enhancing the homeostasis of energy metabolism.
    Article Snippet: Although supplementation with docosahexaenoic acid (DHA) during porcine oocyte IVM is well-established, the available data are limited due to the lack of consistency.. Moreover, to our knowledge, the anti-oxidant effects of DHA on porcine oocytes have not been reported.. Hence, this study aimed to examine the effects of DHA supplementation on the regulation of energy metabolism during porcine oocyte maturation to improve oocyte maturation and embryonic development.

    Article Title: Multifunctional and immunoregulatory microenvironment-responsive hydrogel for whole course management of infected diabetic wounds
    Article Snippet: The cells were then observed under a fluorescence microscope (Ti2, Nikon, Tokyo, Japan).

    Microscopy:

    Article Title: Tetrahedral framework nucleic acids-based delivery of microRNA-155 alleviates intervertebral disc degeneration through targeting Bcl-2/Bax apoptosis pathway.
    Article Snippet: Finally, the samples were observed under a fluorescence microscope (Ti2, Nikon, Japan).

    Article Title: 3D bio scaffold support osteogenic differentiation of mesenchymal stem cells.
    Article Snippet: Funding information Higher Education Commission, Pakistan, Grant/Award Number: 7083 Abstract The regeneration of osteochondral lesions by tissue engineering techniques is challenging due to the lack of physicochemical characteristics and dual‐lineage (osteogenesis and chondrogenesis).. A scaffold with better mechanical properties and dual lineage capability is required for the regeneration of osteochondral defects.. In this study, a hydrogel prepared from decellularized human umbilical cord tissue was developed and evaluated for osteochondral regeneration.

    Article Title: Bone morphogenetic protein 15 gene disruption affects the in vitro maturation of porcine oocytes by impairing spindle assembly and organelle function.
    Article Snippet: Bone morphogenetic protein 15 (BMP15) plays a crucial role in the porcine follicular development.. However, its exact functions in the in vitro maturation (IVM) of porcine oocytes remain largely unknown.. Here, through cytoplasmic injection of a preassembled crRNA–tracrRNA–Cas9 ribonucleoprotein complex, we achieved BMP15 disruption in approximately 54 % of the cultured porcine oocytes.

    Article Title: Ru single-atom nanozymes targeting ROS-ferroptosis pathways for enhanced endometrial regeneration in intrauterine adhesion therapy.
    Article Snippet: In brief, cells were stained with DCFH-DA (10 μM) and incubated in a cell incubator at 37 ◦C for 20 min. After three washes with serum-free cell culture medium to eliminate residual DCFH-DA, the cells were examined using a fluorescence microscope (Ti2, Nikon, Japan), following the protocol described elsewhere [79].

    Article Title: A biomimetic human disease model of bacterial keratitis using a cornea-on-a-chip system.
    Article Snippet: Bacterial keratitis is a common form of inflammation caused by the bacterial invasion of the corneal stroma after trauma.. In extreme cases, it can lead to severe visual impairment or even blindness; therefore, timely medical intervention is imperative.. Unfortunately, widespread misuse of antibiotics has led to the development of drug resistance.

    Article Title: Multifunctional and immunoregulatory microenvironment-responsive hydrogel for whole course management of infected diabetic wounds
    Article Snippet: Cells on the underside of the microporous submembrane were visualized under a fluorescence microscope (Ti2, Nikon, Tokyo, Japan).

    Article Title: Docosahexaenoic acid supplementation during porcine oocyte in vitro maturation improves oocyte quality and embryonic development by enhancing the homeostasis of energy metabolism.
    Article Snippet: Although supplementation with docosahexaenoic acid (DHA) during porcine oocyte IVM is well-established, the available data are limited due to the lack of consistency.. Moreover, to our knowledge, the anti-oxidant effects of DHA on porcine oocytes have not been reported.. Hence, this study aimed to examine the effects of DHA supplementation on the regulation of energy metabolism during porcine oocyte maturation to improve oocyte maturation and embryonic development.

    Article Title: Multifunctional and immunoregulatory microenvironment-responsive hydrogel for whole course management of infected diabetic wounds
    Article Snippet: The cells were then observed under a fluorescence microscope (Ti2, Nikon, Tokyo, Japan).

    Adhesive:

    Article Title: Tetrahedral framework nucleic acids-based delivery of microRNA-155 alleviates intervertebral disc degeneration through targeting Bcl-2/Bax apoptosis pathway.
    Article Snippet: Finally, the samples were observed under a fluorescence microscope (Ti2, Nikon, Japan).

    Article Title: 3D bio scaffold support osteogenic differentiation of mesenchymal stem cells.
    Article Snippet: Funding information Higher Education Commission, Pakistan, Grant/Award Number: 7083 Abstract The regeneration of osteochondral lesions by tissue engineering techniques is challenging due to the lack of physicochemical characteristics and dual‐lineage (osteogenesis and chondrogenesis).. A scaffold with better mechanical properties and dual lineage capability is required for the regeneration of osteochondral defects.. In this study, a hydrogel prepared from decellularized human umbilical cord tissue was developed and evaluated for osteochondral regeneration.

    Article Title: Bone morphogenetic protein 15 gene disruption affects the in vitro maturation of porcine oocytes by impairing spindle assembly and organelle function.
    Article Snippet: Bone morphogenetic protein 15 (BMP15) plays a crucial role in the porcine follicular development.. However, its exact functions in the in vitro maturation (IVM) of porcine oocytes remain largely unknown.. Here, through cytoplasmic injection of a preassembled crRNA–tracrRNA–Cas9 ribonucleoprotein complex, we achieved BMP15 disruption in approximately 54 % of the cultured porcine oocytes.

    Article Title: Ru single-atom nanozymes targeting ROS-ferroptosis pathways for enhanced endometrial regeneration in intrauterine adhesion therapy.
    Article Snippet: In brief, cells were stained with DCFH-DA (10 μM) and incubated in a cell incubator at 37 ◦C for 20 min. After three washes with serum-free cell culture medium to eliminate residual DCFH-DA, the cells were examined using a fluorescence microscope (Ti2, Nikon, Japan), following the protocol described elsewhere [79].

    Article Title: A biomimetic human disease model of bacterial keratitis using a cornea-on-a-chip system.
    Article Snippet: Bacterial keratitis is a common form of inflammation caused by the bacterial invasion of the corneal stroma after trauma.. In extreme cases, it can lead to severe visual impairment or even blindness; therefore, timely medical intervention is imperative.. Unfortunately, widespread misuse of antibiotics has led to the development of drug resistance.

    Article Title: Multifunctional and immunoregulatory microenvironment-responsive hydrogel for whole course management of infected diabetic wounds
    Article Snippet: Cells on the underside of the microporous submembrane were visualized under a fluorescence microscope (Ti2, Nikon, Tokyo, Japan).

    Article Title: Docosahexaenoic acid supplementation during porcine oocyte in vitro maturation improves oocyte quality and embryonic development by enhancing the homeostasis of energy metabolism.
    Article Snippet: Although supplementation with docosahexaenoic acid (DHA) during porcine oocyte IVM is well-established, the available data are limited due to the lack of consistency.. Moreover, to our knowledge, the anti-oxidant effects of DHA on porcine oocytes have not been reported.. Hence, this study aimed to examine the effects of DHA supplementation on the regulation of energy metabolism during porcine oocyte maturation to improve oocyte maturation and embryonic development.

    Article Title: Multifunctional and immunoregulatory microenvironment-responsive hydrogel for whole course management of infected diabetic wounds
    Article Snippet: The cells were then observed under a fluorescence microscope (Ti2, Nikon, Tokyo, Japan).

    Software:

    Article Title: Tetrahedral framework nucleic acids-based delivery of microRNA-155 alleviates intervertebral disc degeneration through targeting Bcl-2/Bax apoptosis pathway.
    Article Snippet: Finally, the samples were observed under a fluorescence microscope (Ti2, Nikon, Japan).

    Article Title: 3D bio scaffold support osteogenic differentiation of mesenchymal stem cells.
    Article Snippet: Funding information Higher Education Commission, Pakistan, Grant/Award Number: 7083 Abstract The regeneration of osteochondral lesions by tissue engineering techniques is challenging due to the lack of physicochemical characteristics and dual‐lineage (osteogenesis and chondrogenesis).. A scaffold with better mechanical properties and dual lineage capability is required for the regeneration of osteochondral defects.. In this study, a hydrogel prepared from decellularized human umbilical cord tissue was developed and evaluated for osteochondral regeneration.

    Article Title: Bone morphogenetic protein 15 gene disruption affects the in vitro maturation of porcine oocytes by impairing spindle assembly and organelle function.
    Article Snippet: Bone morphogenetic protein 15 (BMP15) plays a crucial role in the porcine follicular development.. However, its exact functions in the in vitro maturation (IVM) of porcine oocytes remain largely unknown.. Here, through cytoplasmic injection of a preassembled crRNA–tracrRNA–Cas9 ribonucleoprotein complex, we achieved BMP15 disruption in approximately 54 % of the cultured porcine oocytes.

    Article Title: Ru single-atom nanozymes targeting ROS-ferroptosis pathways for enhanced endometrial regeneration in intrauterine adhesion therapy.
    Article Snippet: In brief, cells were stained with DCFH-DA (10 μM) and incubated in a cell incubator at 37 ◦C for 20 min. After three washes with serum-free cell culture medium to eliminate residual DCFH-DA, the cells were examined using a fluorescence microscope (Ti2, Nikon, Japan), following the protocol described elsewhere [79].

    Article Title: A biomimetic human disease model of bacterial keratitis using a cornea-on-a-chip system.
    Article Snippet: Bacterial keratitis is a common form of inflammation caused by the bacterial invasion of the corneal stroma after trauma.. In extreme cases, it can lead to severe visual impairment or even blindness; therefore, timely medical intervention is imperative.. Unfortunately, widespread misuse of antibiotics has led to the development of drug resistance.

    Article Title: Multifunctional and immunoregulatory microenvironment-responsive hydrogel for whole course management of infected diabetic wounds
    Article Snippet: Cells on the underside of the microporous submembrane were visualized under a fluorescence microscope (Ti2, Nikon, Tokyo, Japan).

    Article Title: Docosahexaenoic acid supplementation during porcine oocyte in vitro maturation improves oocyte quality and embryonic development by enhancing the homeostasis of energy metabolism.
    Article Snippet: Although supplementation with docosahexaenoic acid (DHA) during porcine oocyte IVM is well-established, the available data are limited due to the lack of consistency.. Moreover, to our knowledge, the anti-oxidant effects of DHA on porcine oocytes have not been reported.. Hence, this study aimed to examine the effects of DHA supplementation on the regulation of energy metabolism during porcine oocyte maturation to improve oocyte maturation and embryonic development.

    Article Title: Multifunctional and immunoregulatory microenvironment-responsive hydrogel for whole course management of infected diabetic wounds
    Article Snippet: The cells were then observed under a fluorescence microscope (Ti2, Nikon, Tokyo, Japan).



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    Image Search Results


    Synthesis and characterization of BA-HPCS@CGRP microspheres based on microfluidic fabrication. A. Fourier transform infrared spectroscopy spectra of the HPCS, 3-Carboxyphenylboronic acid (BA), and BA-HPCS. B. The hydrogel precursors appear as a liquid macroscopically before gelation. C. The hydrogels appear milky white after photo-crosslinking. D. The imaging of BA-HPCS@CGRP microspheres based on microfluidic chips: macroscopic and microscopic observations. E. Particle size distribution of BA-HPCS@CGRP microspheres. F and G. Representative scanning electron microscope images of BA-HPCS@CGRP microspheres. H. The pore size distribution of lyophilized BA-HPCS@CGRP microspheres. I. The releasing of CGRP from BA-HPCS@CGRP in PBS and different glucose conditions (100 mg/dL, 400 mg/dL). J. Representative live/dead fluorescence images of L929 cells after co-culture with microspheres (green calcein-AM for live cells, red propidium iodide for dead cells). K. The quantitative analysis of L929 cell viability co-cultured with microspheres. ns, no significance. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Materials Today Bio

    Article Title: Sustained-release CGRP microspheres accelerate diabetic wound healing by synergistically promoting neurovascular regeneration through modulation of macrophage and endothelial cell functions

    doi: 10.1016/j.mtbio.2026.103015

    Figure Lengend Snippet: Synthesis and characterization of BA-HPCS@CGRP microspheres based on microfluidic fabrication. A. Fourier transform infrared spectroscopy spectra of the HPCS, 3-Carboxyphenylboronic acid (BA), and BA-HPCS. B. The hydrogel precursors appear as a liquid macroscopically before gelation. C. The hydrogels appear milky white after photo-crosslinking. D. The imaging of BA-HPCS@CGRP microspheres based on microfluidic chips: macroscopic and microscopic observations. E. Particle size distribution of BA-HPCS@CGRP microspheres. F and G. Representative scanning electron microscope images of BA-HPCS@CGRP microspheres. H. The pore size distribution of lyophilized BA-HPCS@CGRP microspheres. I. The releasing of CGRP from BA-HPCS@CGRP in PBS and different glucose conditions (100 mg/dL, 400 mg/dL). J. Representative live/dead fluorescence images of L929 cells after co-culture with microspheres (green calcein-AM for live cells, red propidium iodide for dead cells). K. The quantitative analysis of L929 cell viability co-cultured with microspheres. ns, no significance. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Following the incubation, fluorescence images were captured using a Nikon inverted fluorescence microscope (Nikon, Japan, Modle: Eclipse Ti2-E).

    Techniques: Fourier Transform Infrared Spectroscopy, Spectroscopy, Imaging, Microscopy, Pore Size, Fluorescence, Co-Culture Assay, Cell Culture