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eclipse ni e upright fluorescence microscope  (Nikon)


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    Nikon eclipse ni e upright fluorescence microscope
    Eclipse Ni E Upright Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 59714 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescence+microscope+eclipse+ni-e/Objectives/pmc12863670-72-9-8
    Average 99 stars, based on 59714 article reviews
    eclipse ni e upright fluorescence microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    other:

    Article Title: Duloxetine deteriorates prefrontal noradrenergic pain facilitation, but reduces locus coeruleus activity to restore endogenous analgesia in chronic neuropathic pain state.
    Article Snippet: Fluorescence signals were captured using a fluorescence microscope (eclipse Ni-E, Nikon Co., Tokyo, Japan).

    Article Title: Aloperine protects the testis against testicular ischemia/reperfusion injury in rats.
    Article Snippet: Funding information Sichuan UniversityWest China Hospital Abstract Background:Testicular torsion/detorsion can cause testis loss and infertility.. Aloperine is a major active alkaloid extracted from Sophora alopecuroides Linn.. It has been shown to have organ-protective effects.

    Article Title: Enhanced osteogenic differentiation of human periodontal ligament cells by mature osteoclasts.
    Article Snippet: Objective: Several in vitro studies have shown that reverse signaling from osteoclasts regulates osteoblast differentiation and mineralization.. However, none of these studies have reported the effects of this signaling pathway on periodontal ligament (PDL) cells.. Therefore, in this study, we aimed to investigate the interaction between receptor activators of nuclear factor kappa B (RANK) released from mature human osteoclasts and the membranous RANK ligand (RANKL) in human PDL cells.

    Article Title: Integrated metabolome and transcriptome analysis of maize roots response to different degrees of drought stress.
    Article Snippet: Observations were made using a fluorescence microscope (ECLIPSE NI-E, Nikon, Tokyo) to examine the metaxylem, phloem, endodermis, aerenchyma, exodermis, and cortex.

    Article Title: Impact of PD‑L1 upregulation on immune checkpoint inhibitor efficacy in triple‑negative breast cancer using a 4T1 murine model.
    Article Snippet: Slides were mounted using fluorescence mounting medium (cat. no. S3023; Dako; Agilent Technologies), and representa‐ tive images were captured using a fluorescence microscope (ECLIPSE Ni‐E; Nikon Corporation).

    Article Title: Impact of PD-L1 upregulation on immune checkpoint inhibitor efficacy in triple-negative breast cancer using a 4T1 murine model
    Article Snippet: Slides were mounted using fluorescence mounting medium (cat. no. S3023; Dako; Agilent Technologies), and representative images were captured using a fluorescence microscope (ECLIPSE Ni-E; Nikon Corporation).

    Article Title: ATR/Chk1 interacting lncRNA modulates DNA damage response to induce breast cancer chemoresistance.
    Article Snippet: The evaluate the immunostaining positive cells was evaluated at least in 10 random fields under a confocal laser-scanning microscope (63 , TCS-SP5, Leica) or fluorescence microscope (20 , Eclipse Ni-E, Nikon).

    Fluorescence:

    Article Title: A neural circuit from thalamic paraventricular nucleus via zona incerta to periaqueductal gray for the facilitation of neuropathic pain.
    Article Snippet: After rinsing with PBS, slices were mounted with a mounting medium (Beyotime). .. Subsequently, the sections were examined using a Nikon fluorescence microscope (Eclipse Ni-E, Nikon, Japan), and images were captured with a Nikon DS-Qi2 camera. .. For primary antibodies, we used antibodies against CaMKIIα (mouse, 1:100; abcam, ab22609), GABA (rabbit, 1:800; Sigma, A2052).

    Microscopy:

    Article Title: A neural circuit from thalamic paraventricular nucleus via zona incerta to periaqueductal gray for the facilitation of neuropathic pain.
    Article Snippet: After rinsing with PBS, slices were mounted with a mounting medium (Beyotime). .. Subsequently, the sections were examined using a Nikon fluorescence microscope (Eclipse Ni-E, Nikon, Japan), and images were captured with a Nikon DS-Qi2 camera. .. For primary antibodies, we used antibodies against CaMKIIα (mouse, 1:100; abcam, ab22609), GABA (rabbit, 1:800; Sigma, A2052).



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    eclipse ni e upright fluorescence microscope  (Nikon)
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    a Phase contrast and H&E staining of NC- and BA-MBOs. Three experiments were repeated with similar results. b Immunostaining of CK19 (green), CD31 (white), VIM (red) and Nucleus (blue; DAPI) in MBOs. Abundance of CK19 + cells ( n = 12 fields for NC or 14 fields for BA examined over 3 independent samples) and average size of gland-like structures ( n = 11 fields for NC or 7 fields for BA examined over 3 independent samples) were quantified (Mean ± SD). c Immunostaining of ZO1 (green), β-catenin (red), CK19 (white) and nucleus (blue; DAPI) in MBOs. Graphs show CK19 + cells exhibiting apical-basal polarity (Mean ± SD; n = 12 fields per 3 organoids). d Alcian Blue staining for MBOs. Mucin production in epithelium was determined by ratio of Alcian Blue + areas in CK19 + (from separately examined <t>immunofluorescence</t> data) areas (Mean ± SD; n = 15 fields [for NC] or 12 fields [for BA] examined over 3 independent samples). e Heatmap (left) and Volcano plot (right) of differentially expressed genes between NC- and BA-MBOs (up in BA: 350; down in BA: 851). f DAB staining of MBOs for MMP-7 and OPN. The staining intensities were quantified by imaging analysis (Mean ± SD; n = 12 fields per 3 organoids). g Representative plots and quantification of FACS for hybrid cells (pan-CK + /VIM + ) and cholangiocytes (pan-CK + /VIM − ) in Mono and MBO. The number and cell ratio were quantified (Mean ± SD; n = 6). For b – d , f , g , the p -values were shown in plots and were determined using two-tailed Welch’s t -test.
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    a Phase contrast and H&E staining of NC- and BA-MBOs. Three experiments were repeated with similar results. b Immunostaining of CK19 (green), CD31 (white), VIM (red) and Nucleus (blue; DAPI) in MBOs. Abundance of CK19 + cells ( n = 12 fields for NC or 14 fields for BA examined over 3 independent samples) and average size of gland-like structures ( n = 11 fields for NC or 7 fields for BA examined over 3 independent samples) were quantified (Mean ± SD). c Immunostaining of ZO1 (green), β-catenin (red), CK19 (white) and nucleus (blue; DAPI) in MBOs. Graphs show CK19 + cells exhibiting apical-basal polarity (Mean ± SD; n = 12 fields per 3 organoids). d Alcian Blue staining for MBOs. Mucin production in epithelium was determined by ratio of Alcian Blue + areas in CK19 + (from separately examined <t>immunofluorescence</t> data) areas (Mean ± SD; n = 15 fields [for NC] or 12 fields [for BA] examined over 3 independent samples). e Heatmap (left) and Volcano plot (right) of differentially expressed genes between NC- and BA-MBOs (up in BA: 350; down in BA: 851). f DAB staining of MBOs for MMP-7 and OPN. The staining intensities were quantified by imaging analysis (Mean ± SD; n = 12 fields per 3 organoids). g Representative plots and quantification of FACS for hybrid cells (pan-CK + /VIM + ) and cholangiocytes (pan-CK + /VIM − ) in Mono and MBO. The number and cell ratio were quantified (Mean ± SD; n = 6). For b – d , f , g , the p -values were shown in plots and were determined using two-tailed Welch’s t -test.
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    Image Search Results


    a Phase contrast and H&E staining of NC- and BA-MBOs. Three experiments were repeated with similar results. b Immunostaining of CK19 (green), CD31 (white), VIM (red) and Nucleus (blue; DAPI) in MBOs. Abundance of CK19 + cells ( n = 12 fields for NC or 14 fields for BA examined over 3 independent samples) and average size of gland-like structures ( n = 11 fields for NC or 7 fields for BA examined over 3 independent samples) were quantified (Mean ± SD). c Immunostaining of ZO1 (green), β-catenin (red), CK19 (white) and nucleus (blue; DAPI) in MBOs. Graphs show CK19 + cells exhibiting apical-basal polarity (Mean ± SD; n = 12 fields per 3 organoids). d Alcian Blue staining for MBOs. Mucin production in epithelium was determined by ratio of Alcian Blue + areas in CK19 + (from separately examined immunofluorescence data) areas (Mean ± SD; n = 15 fields [for NC] or 12 fields [for BA] examined over 3 independent samples). e Heatmap (left) and Volcano plot (right) of differentially expressed genes between NC- and BA-MBOs (up in BA: 350; down in BA: 851). f DAB staining of MBOs for MMP-7 and OPN. The staining intensities were quantified by imaging analysis (Mean ± SD; n = 12 fields per 3 organoids). g Representative plots and quantification of FACS for hybrid cells (pan-CK + /VIM + ) and cholangiocytes (pan-CK + /VIM − ) in Mono and MBO. The number and cell ratio were quantified (Mean ± SD; n = 6). For b – d , f , g , the p -values were shown in plots and were determined using two-tailed Welch’s t -test.

    Journal: Nature Communications

    Article Title: Cellular crosstalk mediated by TGF-β drives epithelial-mesenchymal transition in patient-derived multi-compartment biliary organoids

    doi: 10.1038/s41467-025-61442-5

    Figure Lengend Snippet: a Phase contrast and H&E staining of NC- and BA-MBOs. Three experiments were repeated with similar results. b Immunostaining of CK19 (green), CD31 (white), VIM (red) and Nucleus (blue; DAPI) in MBOs. Abundance of CK19 + cells ( n = 12 fields for NC or 14 fields for BA examined over 3 independent samples) and average size of gland-like structures ( n = 11 fields for NC or 7 fields for BA examined over 3 independent samples) were quantified (Mean ± SD). c Immunostaining of ZO1 (green), β-catenin (red), CK19 (white) and nucleus (blue; DAPI) in MBOs. Graphs show CK19 + cells exhibiting apical-basal polarity (Mean ± SD; n = 12 fields per 3 organoids). d Alcian Blue staining for MBOs. Mucin production in epithelium was determined by ratio of Alcian Blue + areas in CK19 + (from separately examined immunofluorescence data) areas (Mean ± SD; n = 15 fields [for NC] or 12 fields [for BA] examined over 3 independent samples). e Heatmap (left) and Volcano plot (right) of differentially expressed genes between NC- and BA-MBOs (up in BA: 350; down in BA: 851). f DAB staining of MBOs for MMP-7 and OPN. The staining intensities were quantified by imaging analysis (Mean ± SD; n = 12 fields per 3 organoids). g Representative plots and quantification of FACS for hybrid cells (pan-CK + /VIM + ) and cholangiocytes (pan-CK + /VIM − ) in Mono and MBO. The number and cell ratio were quantified (Mean ± SD; n = 6). For b – d , f , g , the p -values were shown in plots and were determined using two-tailed Welch’s t -test.

    Article Snippet: Bright field images were captured using a light microscope (Olympus, BX43) and immunofluorescence images were captured using either fluorescence (Olympus, BX51; Nikon, ECLIPSE Ni-E) or confocal (Nikon, ECLIPSE Ti; Zeiss, LSM880) microscopes.

    Techniques: Staining, Immunostaining, Immunofluorescence, Imaging, Two Tailed Test

    a Overview of in vitro A8301-mediated TGF-β signal inhibition in BA-MBOs. Created in BioRender. Ayabe, H. (2025) https://BioRender.com/q1pk62z . b Representative plots and quantification of FACS of day 14 MBOs treated with A8301. Number of hybrid (pan-CK + /VIM + ), cholangiocyte (pan-CK + /VIM - ) and mesenchymal (pan-CK − /VIM + ) cells were quantified (Mean ± SD; n = 12 organoids). c Images of MBOs treated with A8301 captured before collection and after whole-mount immunostaining (CK19: red, CD31: green, VIM: white). Abundance of CK19 + structures (Mean ± SD; n = 9 organoids examined over 3 independent experiments) and size distribution of PBG-like structures were quantified ( n = 39-104 clusters examined over 3 independent experiments). d Immunostaining of ECAD (green), CK19 (red) and Nucleus (blue; DAPI) in MBOs treated with A8301. ECAD + cells in CK19 + cells in the field were quantified (Mean ± SD; n = 12 fields from 3 organoids). e Immunostaining of ZO1 (green), CK19 (red) and Nucleus (blue; DAPI) in MBO treated with A8301. ZO1 + cells in CK19 + cells per field were quantified (Mean ± SD; n = 12 fields from 3 organoids). f Alcian blue staining of MBOs treated with A8301. Mucin production was determined by ratio of Alcian Blue + areas in CK19 + (from separately examined immunofluorescence data) areas (Mean ± SD; n = 12 fields from 3 organoids). g Top enriched terms of MSigDB Hallmark 2020 , and GO , sets in serum proteins (reanalyzed from Lertudomphonwanit et al. ) upregulated in BA subjects ( n = 175 patients) vs age-matched normal controls ( n = 9). MF: Molecular Function, BP: Biological Process. h Heatmap of enriched proteins in the EMT pathway in MSigDB Hallmark 2020 sets. ( n = 9 NC and 175 BA subjects). i Serum TGF-β/Activin ligand levels represented by the relative fluorescent unit (RFU; Mean ± SD). ( n = 9 NC and 175 BA subjects). The p -values were shown in plots and were determined using one-way ANOVA with Tukey’s test ( b – f ) and two-tailed Welch’s t -test ( i ).

    Journal: Nature Communications

    Article Title: Cellular crosstalk mediated by TGF-β drives epithelial-mesenchymal transition in patient-derived multi-compartment biliary organoids

    doi: 10.1038/s41467-025-61442-5

    Figure Lengend Snippet: a Overview of in vitro A8301-mediated TGF-β signal inhibition in BA-MBOs. Created in BioRender. Ayabe, H. (2025) https://BioRender.com/q1pk62z . b Representative plots and quantification of FACS of day 14 MBOs treated with A8301. Number of hybrid (pan-CK + /VIM + ), cholangiocyte (pan-CK + /VIM - ) and mesenchymal (pan-CK − /VIM + ) cells were quantified (Mean ± SD; n = 12 organoids). c Images of MBOs treated with A8301 captured before collection and after whole-mount immunostaining (CK19: red, CD31: green, VIM: white). Abundance of CK19 + structures (Mean ± SD; n = 9 organoids examined over 3 independent experiments) and size distribution of PBG-like structures were quantified ( n = 39-104 clusters examined over 3 independent experiments). d Immunostaining of ECAD (green), CK19 (red) and Nucleus (blue; DAPI) in MBOs treated with A8301. ECAD + cells in CK19 + cells in the field were quantified (Mean ± SD; n = 12 fields from 3 organoids). e Immunostaining of ZO1 (green), CK19 (red) and Nucleus (blue; DAPI) in MBO treated with A8301. ZO1 + cells in CK19 + cells per field were quantified (Mean ± SD; n = 12 fields from 3 organoids). f Alcian blue staining of MBOs treated with A8301. Mucin production was determined by ratio of Alcian Blue + areas in CK19 + (from separately examined immunofluorescence data) areas (Mean ± SD; n = 12 fields from 3 organoids). g Top enriched terms of MSigDB Hallmark 2020 , and GO , sets in serum proteins (reanalyzed from Lertudomphonwanit et al. ) upregulated in BA subjects ( n = 175 patients) vs age-matched normal controls ( n = 9). MF: Molecular Function, BP: Biological Process. h Heatmap of enriched proteins in the EMT pathway in MSigDB Hallmark 2020 sets. ( n = 9 NC and 175 BA subjects). i Serum TGF-β/Activin ligand levels represented by the relative fluorescent unit (RFU; Mean ± SD). ( n = 9 NC and 175 BA subjects). The p -values were shown in plots and were determined using one-way ANOVA with Tukey’s test ( b – f ) and two-tailed Welch’s t -test ( i ).

    Article Snippet: Bright field images were captured using a light microscope (Olympus, BX43) and immunofluorescence images were captured using either fluorescence (Olympus, BX51; Nikon, ECLIPSE Ni-E) or confocal (Nikon, ECLIPSE Ti; Zeiss, LSM880) microscopes.

    Techniques: In Vitro, Inhibition, Immunostaining, Staining, Immunofluorescence, Two Tailed Test