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eclipse 90i fluorescence microscope  (Nikon)


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    Structured Review

    Nikon eclipse 90i fluorescence microscope
    Eclipse 90i Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 1657 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescence+filter+cubes/Fluorescence+Filter+Cubes/10__1007_slash_s44411___026___00558___6-96-5-9
    Average 96 stars, based on 1657 article reviews
    eclipse 90i fluorescence microscope - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Exploring single-cell biosynthetic noise and dynamics for enhanced betaxanthin production in Escherichia coli.
    Article Snippet: Microscope fluorescence and phase contrast images were collected on a Nikon Tieclipse (Melville, NY, U.S.A.) equipped with the Nikon Perfect Focus (PFS) unit, an Evolve 512 EMCCD camera from Photometrics (Huntington Beach, CA, U.S.A.) and a Tokai Hit Stage Top Incubation Systems (Fujinomiya, Japan). .. Illumination for fluorescence was provided by a white light LED source (X-Cite 120 LED, Lumen Dynamics, Mississauga, ON, Canada) transmitted through fluorescence filter cubes (DS-Red channel: excitation: 540/551 nm, emission: 567/642 nm; YFP channel: excitation: 490/510 nm, emission 520/550) and an oil immersion 100x objective (Nikon). ..

    Article Title: A wave of monocytes is recruited to replenish the long-term Langerhans cell network after immune injury.
    Article Snippet: .. 0.21mm), using a C2 confocal scan head with488nm and 561/568nm optimized fluorescence filter cubes (Nikon Instruments, Tokyo, Japan). ..

    Article Title: Exploring single-cell biosynthetic noise and dynamics for enhanced betaxanthin production in Escherichia coli
    Article Snippet: Microscope fluorescence and phase contrast images were collected on a Nikon Ti-eclipse (Melville, NY, USA) equipped with the Nikon Perfect Focus (PFS) unit, an Evolve 512 EMCCD camera from Photometrics (Huntington Beach, CA, USA), and a Tokai Hit Stage Top Incubation Systems (Fujinomiya, Japan). .. Illumination for fluorescence was provided by a white light LED source (X-Cite 120 LED, Lumen Dynamics, Mississauga, ON, Canada) transmitted through fluorescence filter cubes (DS-Red channel: excitation: 540/551 nm, emission: 567/642 nm; YFP channel: excitation: 490/510 nm, emission 520/550) and an oil immersion 100× objective (Nikon). ..

    Article Title: Leukocyte-Derived Extracellular Vesicles in Blood with and without EpCAM Enrichment
    Article Snippet: .. Image data sets of 55–65 frames/channel were acquired to cover the whole surface of each well using a semi-automated inverted fluorescence microscope (Eclipse Ti-E, Nikon Instruments, Amsterdam, The Netherlands) equipped with a 10×/0.45 numerical aperture (NA) objective, a camera (Orca flash 4.0 LT, C11440, Hamamatsu, Almere, The Netherlands) and fluorescence filter cubes (DAPI, FITC, PerCP, APC filter sets for the detection of Hoechst, CD61-Alexa 488, CD45-PerCP and CD235a-Alexa 647, respectively). ..

    Article Title: Few-cycle all-fiber supercontinuum laser for ultrabroadband multimodal nonlinear microscopy
    Article Snippet: Possible leakage of fundamental laser light was blocked with a 350 - 700 nm bandpass BG40 filter (FGB37-A, Thorlabs, filter 1 in Fig. 6(a)). .. The two-photon excited fluorescence (TPEF) signal was filtered with fluorescence filter cubes (DAPI, FITC and TRITC: Standard series, Nikon) and collected in the backward direction. .. We used a 25x NA1.10 water immersion objective (Apo LWD, Nikon) for the second harmonic generation (SHG) signal collection in the forward direction.

    Article Title: Endocytic regulation of cellular ion homeostasis controls lysosome biogenesis.
    Article Snippet: .. 1Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany.. 2Faculty of Biology, Chemistry, and Pharmacy, Freie Universität Berlin, Berlin, Germany.. ✉e-mail: lopezhernandez@fmp-berlin.de; maritzen@fmp-berlin.de; haucke@fmp-berlin.de Late endosomes, autophagosomes/autolysosomes and lysosomes coordinate the degradative turnover of macromolecules with cell metabolism1 by responding to intracellular cues (for example, amino acid levels) and extracellular signals (for example, insulin)2,3. ..

    Article Title: Fine-tuned spatiotemporal dynamics of DNA replication during phage lambda infection.
    Article Snippet: After the ejection of viral DNA into the host cytoplasm, the temper ate bacteriophage (phage) lambda integrates a cascade of expressions from various regulatory genes, coupled with DNA replication, to commit to a decision between lysis and lysogeny.. Higher multiplicity of infection (MOI) greatly shifts the decision toward the lysogenic pathway.. However, how the phage separates the MOI from replicated viral DNA during lysis–lysogeny decision-making is unclear.

    Microscopy:

    Article Title: Leukocyte-Derived Extracellular Vesicles in Blood with and without EpCAM Enrichment
    Article Snippet: .. Image data sets of 55–65 frames/channel were acquired to cover the whole surface of each well using a semi-automated inverted fluorescence microscope (Eclipse Ti-E, Nikon Instruments, Amsterdam, The Netherlands) equipped with a 10×/0.45 numerical aperture (NA) objective, a camera (Orca flash 4.0 LT, C11440, Hamamatsu, Almere, The Netherlands) and fluorescence filter cubes (DAPI, FITC, PerCP, APC filter sets for the detection of Hoechst, CD61-Alexa 488, CD45-PerCP and CD235a-Alexa 647, respectively). ..

    Article Title: Endocytic regulation of cellular ion homeostasis controls lysosome biogenesis.
    Article Snippet: .. 1Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany.. 2Faculty of Biology, Chemistry, and Pharmacy, Freie Universität Berlin, Berlin, Germany.. ✉e-mail: lopezhernandez@fmp-berlin.de; maritzen@fmp-berlin.de; haucke@fmp-berlin.de Late endosomes, autophagosomes/autolysosomes and lysosomes coordinate the degradative turnover of macromolecules with cell metabolism1 by responding to intracellular cues (for example, amino acid levels) and extracellular signals (for example, insulin)2,3. ..

    Software:

    Article Title: Endocytic regulation of cellular ion homeostasis controls lysosome biogenesis.
    Article Snippet: .. 1Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany.. 2Faculty of Biology, Chemistry, and Pharmacy, Freie Universität Berlin, Berlin, Germany.. ✉e-mail: lopezhernandez@fmp-berlin.de; maritzen@fmp-berlin.de; haucke@fmp-berlin.de Late endosomes, autophagosomes/autolysosomes and lysosomes coordinate the degradative turnover of macromolecules with cell metabolism1 by responding to intracellular cues (for example, amino acid levels) and extracellular signals (for example, insulin)2,3. ..

    Fluorescence In Situ Hybridization:

    Article Title: Fine-tuned spatiotemporal dynamics of DNA replication during phage lambda infection.
    Article Snippet: After the ejection of viral DNA into the host cytoplasm, the temper ate bacteriophage (phage) lambda integrates a cascade of expressions from various regulatory genes, coupled with DNA replication, to commit to a decision between lysis and lysogeny.. Higher multiplicity of infection (MOI) greatly shifts the decision toward the lysogenic pathway.. However, how the phage separates the MOI from replicated viral DNA during lysis–lysogeny decision-making is unclear.



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    Nikon fluorescence filter cubes
    a The betaxanthin biosynthetic pathway. L-DOPA is provided to the cell media and is converted to betalamic acid by DOD which is fused to mCherry. Betalamic acid spontaneously reacts with primary amines to form yellow fluorescent betaxanthins. b Microfluidics chip and representative image of a single chamber within the microfluidics chip where the cells were maintained at the steady state. P1 is the inlet of cell culture, P2 is the inlet of fresh media, and P3 is the outlet of waste. c Representative time-lapse <t>fluorescence</t> microscopy images for measuring single-cell growth (top), protein level (middle), and metabolite level (bottom). Source data are provided as a Source Data file.
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    Image Search Results


    a The betaxanthin biosynthetic pathway. L-DOPA is provided to the cell media and is converted to betalamic acid by DOD which is fused to mCherry. Betalamic acid spontaneously reacts with primary amines to form yellow fluorescent betaxanthins. b Microfluidics chip and representative image of a single chamber within the microfluidics chip where the cells were maintained at the steady state. P1 is the inlet of cell culture, P2 is the inlet of fresh media, and P3 is the outlet of waste. c Representative time-lapse fluorescence microscopy images for measuring single-cell growth (top), protein level (middle), and metabolite level (bottom). Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Exploring single-cell biosynthetic noise and dynamics for enhanced betaxanthin production in Escherichia coli

    doi: 10.1038/s41467-025-67733-1

    Figure Lengend Snippet: a The betaxanthin biosynthetic pathway. L-DOPA is provided to the cell media and is converted to betalamic acid by DOD which is fused to mCherry. Betalamic acid spontaneously reacts with primary amines to form yellow fluorescent betaxanthins. b Microfluidics chip and representative image of a single chamber within the microfluidics chip where the cells were maintained at the steady state. P1 is the inlet of cell culture, P2 is the inlet of fresh media, and P3 is the outlet of waste. c Representative time-lapse fluorescence microscopy images for measuring single-cell growth (top), protein level (middle), and metabolite level (bottom). Source data are provided as a Source Data file.

    Article Snippet: Illumination for fluorescence was provided by a white light LED source (X-Cite 120 LED, Lumen Dynamics, Mississauga, ON, Canada) transmitted through fluorescence filter cubes (DS-Red channel: excitation: 540/551 nm, emission: 567/642 nm; YFP channel: excitation: 490/510 nm, emission 520/550) and an oil immersion 100× objective (Nikon).

    Techniques: Cell Culture, Fluorescence, Microscopy

    a Schematic of the in vivo Egos circuit. FmdA was placed downstream of a DOD-mCherry fusion with an RBS library to couple DOD expression noise with ammonium availability. b RBS sequences and their corresponding translation initiation rates of the four Egos variants. c , d Representative single-cell fluorescence microscopy images with 1 μm scale bar showing mCherry and betaxanthin levels for the open-loop control and each Egos variant. e , f Probability density distributions of protein (mCherry) and metabolite (betaxanthin) concentrations calculated from fluorescent microscopy. g Growth curves of Egos variants and the open-loop control (lacking fmdA ) in formamide-based selective medium. h Quantification of total betaxanthin titer. Data represents the mean ± s.d. of biological replicates ( n = 3). ** indicates p ≤ 0.01 ( p = 0.0073 for Egos4), and *** indicates p ≤ 0.001 ( p = 0.00039 for Egos2 and p = 0.00027 for Egos3) from two-tailed t -test. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Exploring single-cell biosynthetic noise and dynamics for enhanced betaxanthin production in Escherichia coli

    doi: 10.1038/s41467-025-67733-1

    Figure Lengend Snippet: a Schematic of the in vivo Egos circuit. FmdA was placed downstream of a DOD-mCherry fusion with an RBS library to couple DOD expression noise with ammonium availability. b RBS sequences and their corresponding translation initiation rates of the four Egos variants. c , d Representative single-cell fluorescence microscopy images with 1 μm scale bar showing mCherry and betaxanthin levels for the open-loop control and each Egos variant. e , f Probability density distributions of protein (mCherry) and metabolite (betaxanthin) concentrations calculated from fluorescent microscopy. g Growth curves of Egos variants and the open-loop control (lacking fmdA ) in formamide-based selective medium. h Quantification of total betaxanthin titer. Data represents the mean ± s.d. of biological replicates ( n = 3). ** indicates p ≤ 0.01 ( p = 0.0073 for Egos4), and *** indicates p ≤ 0.001 ( p = 0.00039 for Egos2 and p = 0.00027 for Egos3) from two-tailed t -test. Source data are provided as a Source Data file.

    Article Snippet: Illumination for fluorescence was provided by a white light LED source (X-Cite 120 LED, Lumen Dynamics, Mississauga, ON, Canada) transmitted through fluorescence filter cubes (DS-Red channel: excitation: 540/551 nm, emission: 567/642 nm; YFP channel: excitation: 490/510 nm, emission 520/550) and an oil immersion 100× objective (Nikon).

    Techniques: In Vivo, Expressing, Fluorescence, Microscopy, Control, Variant Assay, Two Tailed Test