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flow cytometry data analyses  (GraphPad Software Inc)


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    GraphPad Software Inc flow cytometry data analyses
    Lymphocyte profiling of the L-TME in pediatric AML patients. (a) Logical gating strategy showing the T cell composition of L-TME in AML patients with (top row, n=4 ) and without (bottom row, n=5 ) sustained-CR. The data are displayed cumulatively in the dot plots and individually in the graphs (c and e). The same cumulative and individual representation is depicted in all figures showing <t>flow-cytometry</t> data. (b) I) Cumulative plots displaying CD45 + /CD34 − /CD19 − and CD3 − myeloid cells in AML patients with (top row, n=4 ) and without (bottom row, n=5 ) sustained-CR. II-III) Individual values of samples in (a) and (b) are displayed in a graph; Q value is the result of FDR-corrected multiple t-tests between sustained (red) and non-sustained (black) patients’ samples. Only significant differences are shown. IV) The % of blasts scored in the patients’ diagnosis for all the cohort is displayed in the graph for patients with and without sustained-CR. The blast score was calculated by a combination of microscope and clinical flow-cytometry analyses and provided as clinical report for patients in the Pathology Laboratory of Sidra. (c) I) Phenotype of the CD4 dim cell population: the majority of the cells are IFNγ + (shown in the colored Z-axis) CD4 + and CD8 + doublets, engaging in close networking interactions. A full panel of expression markers for this population is shown in Supplementary Figure 1b. II) Individual values of samples in (b) show a trend of enrichment in CD4 dim doublets (considering lower singlets) in patients without long-term remission.
    Flow Cytometry Data Analyses, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flow+cytometry+data+analyses/flow+cytometry/bio_rxiv__2025__07__17__664572-53-4-10
    Average 90 stars, based on 1 article reviews
    flow cytometry data analyses - by Bioz Stars, 2026-10
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    1) Product Images from "A 3-genes interferon signature predicts sustained complete remission in pediatric AML patients"

    Article Title: A 3-genes interferon signature predicts sustained complete remission in pediatric AML patients

    Journal: bioRxiv

    doi: 10.1101/2025.07.17.664572

    Lymphocyte profiling of the L-TME in pediatric AML patients. (a) Logical gating strategy showing the T cell composition of L-TME in AML patients with (top row, n=4 ) and without (bottom row, n=5 ) sustained-CR. The data are displayed cumulatively in the dot plots and individually in the graphs (c and e). The same cumulative and individual representation is depicted in all figures showing flow-cytometry data. (b) I) Cumulative plots displaying CD45 + /CD34 − /CD19 − and CD3 − myeloid cells in AML patients with (top row, n=4 ) and without (bottom row, n=5 ) sustained-CR. II-III) Individual values of samples in (a) and (b) are displayed in a graph; Q value is the result of FDR-corrected multiple t-tests between sustained (red) and non-sustained (black) patients’ samples. Only significant differences are shown. IV) The % of blasts scored in the patients’ diagnosis for all the cohort is displayed in the graph for patients with and without sustained-CR. The blast score was calculated by a combination of microscope and clinical flow-cytometry analyses and provided as clinical report for patients in the Pathology Laboratory of Sidra. (c) I) Phenotype of the CD4 dim cell population: the majority of the cells are IFNγ + (shown in the colored Z-axis) CD4 + and CD8 + doublets, engaging in close networking interactions. A full panel of expression markers for this population is shown in Supplementary Figure 1b. II) Individual values of samples in (b) show a trend of enrichment in CD4 dim doublets (considering lower singlets) in patients without long-term remission.
    Figure Legend Snippet: Lymphocyte profiling of the L-TME in pediatric AML patients. (a) Logical gating strategy showing the T cell composition of L-TME in AML patients with (top row, n=4 ) and without (bottom row, n=5 ) sustained-CR. The data are displayed cumulatively in the dot plots and individually in the graphs (c and e). The same cumulative and individual representation is depicted in all figures showing flow-cytometry data. (b) I) Cumulative plots displaying CD45 + /CD34 − /CD19 − and CD3 − myeloid cells in AML patients with (top row, n=4 ) and without (bottom row, n=5 ) sustained-CR. II-III) Individual values of samples in (a) and (b) are displayed in a graph; Q value is the result of FDR-corrected multiple t-tests between sustained (red) and non-sustained (black) patients’ samples. Only significant differences are shown. IV) The % of blasts scored in the patients’ diagnosis for all the cohort is displayed in the graph for patients with and without sustained-CR. The blast score was calculated by a combination of microscope and clinical flow-cytometry analyses and provided as clinical report for patients in the Pathology Laboratory of Sidra. (c) I) Phenotype of the CD4 dim cell population: the majority of the cells are IFNγ + (shown in the colored Z-axis) CD4 + and CD8 + doublets, engaging in close networking interactions. A full panel of expression markers for this population is shown in Supplementary Figure 1b. II) Individual values of samples in (b) show a trend of enrichment in CD4 dim doublets (considering lower singlets) in patients without long-term remission.

    Techniques Used: Flow Cytometry, Biomarker Discovery, Microscopy, Expressing

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    Article Title: EGFR -Mutant Lung Adenocarcinoma Cell-Derived Exosomal miR-651-5p Induces CD8+ T Cell Apoptosis via Downregulating BCL2 Expression.
    Article Snippet: .. Flow cytometry data analyses were performed using GraphPad Prism7.0 (San Diego, CA, USA). ..

    Article Title: A 3-genes interferon signature predicts sustained complete remission in pediatric AML patients
    Article Snippet: .. Statistical analyses of the flow cytometry data were performed using GraphPad Prism (v. 10.3.1). .. We performed DEG analysis using the NanoString assay on the Discovery Cohort between patients with sustained-CR ( n=6 ) vs non-sustained CR ( n=19 ), and identified a clear distinction between myeloid suppression-related genes and a T-cell infiltration signature represented by 67 DEGs ( p ≤ 0.05) ( ) (Supplementary Table 4).



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    Lymphocyte profiling of the L-TME in pediatric AML patients. (a) Logical gating strategy showing the T cell composition of L-TME in AML patients with (top row, n=4 ) and without (bottom row, n=5 ) sustained-CR. The data are displayed cumulatively in the dot plots and individually in the graphs (c and e). The same cumulative and individual representation is depicted in all figures showing <t>flow-cytometry</t> data. (b) I) Cumulative plots displaying CD45 + /CD34 − /CD19 − and CD3 − myeloid cells in AML patients with (top row, n=4 ) and without (bottom row, n=5 ) sustained-CR. II-III) Individual values of samples in (a) and (b) are displayed in a graph; Q value is the result of FDR-corrected multiple t-tests between sustained (red) and non-sustained (black) patients’ samples. Only significant differences are shown. IV) The % of blasts scored in the patients’ diagnosis for all the cohort is displayed in the graph for patients with and without sustained-CR. The blast score was calculated by a combination of microscope and clinical flow-cytometry analyses and provided as clinical report for patients in the Pathology Laboratory of Sidra. (c) I) Phenotype of the CD4 dim cell population: the majority of the cells are IFNγ + (shown in the colored Z-axis) CD4 + and CD8 + doublets, engaging in close networking interactions. A full panel of expression markers for this population is shown in Supplementary Figure 1b. II) Individual values of samples in (b) show a trend of enrichment in CD4 dim doublets (considering lower singlets) in patients without long-term remission.
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    Lymphocyte profiling of the L-TME in pediatric AML patients. (a) Logical gating strategy showing the T cell composition of L-TME in AML patients with (top row, n=4 ) and without (bottom row, n=5 ) sustained-CR. The data are displayed cumulatively in the dot plots and individually in the graphs (c and e). The same cumulative and individual representation is depicted in all figures showing flow-cytometry data. (b) I) Cumulative plots displaying CD45 + /CD34 − /CD19 − and CD3 − myeloid cells in AML patients with (top row, n=4 ) and without (bottom row, n=5 ) sustained-CR. II-III) Individual values of samples in (a) and (b) are displayed in a graph; Q value is the result of FDR-corrected multiple t-tests between sustained (red) and non-sustained (black) patients’ samples. Only significant differences are shown. IV) The % of blasts scored in the patients’ diagnosis for all the cohort is displayed in the graph for patients with and without sustained-CR. The blast score was calculated by a combination of microscope and clinical flow-cytometry analyses and provided as clinical report for patients in the Pathology Laboratory of Sidra. (c) I) Phenotype of the CD4 dim cell population: the majority of the cells are IFNγ + (shown in the colored Z-axis) CD4 + and CD8 + doublets, engaging in close networking interactions. A full panel of expression markers for this population is shown in Supplementary Figure 1b. II) Individual values of samples in (b) show a trend of enrichment in CD4 dim doublets (considering lower singlets) in patients without long-term remission.

    Journal: bioRxiv

    Article Title: A 3-genes interferon signature predicts sustained complete remission in pediatric AML patients

    doi: 10.1101/2025.07.17.664572

    Figure Lengend Snippet: Lymphocyte profiling of the L-TME in pediatric AML patients. (a) Logical gating strategy showing the T cell composition of L-TME in AML patients with (top row, n=4 ) and without (bottom row, n=5 ) sustained-CR. The data are displayed cumulatively in the dot plots and individually in the graphs (c and e). The same cumulative and individual representation is depicted in all figures showing flow-cytometry data. (b) I) Cumulative plots displaying CD45 + /CD34 − /CD19 − and CD3 − myeloid cells in AML patients with (top row, n=4 ) and without (bottom row, n=5 ) sustained-CR. II-III) Individual values of samples in (a) and (b) are displayed in a graph; Q value is the result of FDR-corrected multiple t-tests between sustained (red) and non-sustained (black) patients’ samples. Only significant differences are shown. IV) The % of blasts scored in the patients’ diagnosis for all the cohort is displayed in the graph for patients with and without sustained-CR. The blast score was calculated by a combination of microscope and clinical flow-cytometry analyses and provided as clinical report for patients in the Pathology Laboratory of Sidra. (c) I) Phenotype of the CD4 dim cell population: the majority of the cells are IFNγ + (shown in the colored Z-axis) CD4 + and CD8 + doublets, engaging in close networking interactions. A full panel of expression markers for this population is shown in Supplementary Figure 1b. II) Individual values of samples in (b) show a trend of enrichment in CD4 dim doublets (considering lower singlets) in patients without long-term remission.

    Article Snippet: Statistical analyses of the flow cytometry data were performed using GraphPad Prism (v. 10.3.1).

    Techniques: Flow Cytometry, Biomarker Discovery, Microscopy, Expressing