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proteins
Proteins, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+peptide/FLAG+peptide/pm42409167-137-2-11
Average 95 stars, based on 43 article reviews
Proteins, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+peptide/FLAG+peptide/pm42409167-137-2-11
Average 95 stars, based on 43 article reviews
proteins - by Bioz Stars,
2026-09
95/100 stars
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Lysis:Article Title: Riboflavin metabolism shapes FSP1-driven ferroptosis resistance Article Snippet: For IP of Flag-tagged proteins, equal amounts of total protein (4 mg) were incubated with 50 μl of Pierce Anti-DYKDDDDK magnetic agarose beads (Invitrogen, cat. no. A36797) overnight at 4 °C with gentle rotation. .. Beads were washed three times with lysis buffer and then eluted with 100 μl of 1.5 mg ml −1 3× Article Title: Riboflavin metabolism shapes FSP1-driven ferroptosis resistance. Article Snippet: For IP of Flag-tagged proteins, equal amounts of total protein (4 mg) were incubated with 50 μl of Pierce Anti-DYKDDDDK magnetic agarose beads (Invitrogen, cat. no. A36797) overnight at 4 °C with gentle rotation. .. Beads were washed three times with lysis buffer and then eluted with 100 μl of 1.5 mg ml−1 3× Transfection:Article Title: PRRSV-2 impedes lysosomes from eliminating secondary infected bacteria. Article Snippet: The co-localization of Smurf1, RhoA and nsp5 was analyzed using ImageJ, and the results were quantified using the Pearson’s correlation coefficient. n = 10 cells from three independent experiments. (E) HEK-293T cells were co-transfected with HA-nsp5, Flag- Smurf1 and MycRhoA plasmids for 36 h, the cells were harvested to detect the interaction between nsp5, RhoA and Smurf1 by ternary Co-IP. (F) HEK-293T cells were co-transfected with the plasmids of RhoA, Smurf1 and Ub for 36 h, the cells were harvested to detect the ubiquitination of RhoA induced by Smurf1. (G) HEK-293T cells were co-transfected with the plasmids of RhoA, Smurf1, nsp5 and Ub for 36 h, the cells were harvested to detect the effect of nsp5 on Smurf1-induced polyubiquitination of RhoA. (H) HEK-293T cells were co-transfected with the plasmids of RhoA, Smurf1, nsp5 and K63-Ub for 36 h, the cells were harvested to detect the effect of nsp5 on Smurf1-induced polyubiquitination of RhoA. (I) HEK-293T cells were co-transfected with the plasmids of RhoA, nsp5 and Smurf1 for 36 h, the cells were harvested to detect the RhoA, nsp5 and Smurf1 protein expression by Western blot, tubulin served as an internal control. (J) Smurf1+/+ and Smurf1-/- HEK-293T cells were co-transfected with the plasmids of RhoA and nsp5 for 36 h, the cells were harvested to detect the RhoA, nsp5 and Smurf1 protein expression by Western blot, tubulin served as an internal control. (K) Smurf1-/- HEK-293T cells were co-transfected with the plasmids of RhoA, nsp5 and Smurf1 for 36 h, the cells were harvested to detect the RhoA, nsp5 and Smurf1 protein expression by Western blot, tubulin served as an internal control. (L) PAMs were transfected with siNC and siSmurf1 for 24 h, and then infected with recombinant lentivirus expressing nsp5 or control lentivirus for 24 h, the cells were harvested to detect the RhoA, nsp5 and Smurf1 protein expression by Western blot, tubulin served as an internal control. .. Scale bar: 10 μm. https://doi.org/10.1371/journal.ppat.1014000.g008 PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1014000 February 27, 2026 20 / 27 https://doi.org/10.1371/journal.ppat.1014000.g009 PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1014000 February 27, 2026 21 / 27 were purchased from Proteintech, anti-PRRSV-2 N monoclonal antibody was prepared by our laboratory; Lipofectamine RNAiMAX Transfection Reagent (13778150), Lipofectamine 3000 Transfection Reagent (L3000015) and LysoTracker Red DND-99 (L7528) were purchased from Thermo Fisher Scientific; CFDA (S1076) was purchased from Solarbio; YF 488-Phalloidin (YP0114L) was purchased from UElandy; Latrunculin A (HY-16929), 3 × Article Title: PRRSV-2 impedes lysosomes from eliminating secondary infected bacteria Article Snippet: .. Anti-RhoA monoclonal antibody (#2117) was purchased from Cell Signaling Technology, Anti-Smurf1 (sc-100616) was purchased from Santa Cruz Biotechnology, anti-RhoA antibody (10749–1-AP, 66733–1-Ig), anti-ATG7 polyclonal antibody (10088–2-AP), anti-TOLLIP polyclonal antibody (11315–1-AP), anti-LAMP1 monoclonal antibody (CL647–65051), anti-ubiquitin Polyclonal antibody (80992–1-RR) were purchased from Proteintech, anti-tubulin monoclonal antibody (HC-101) was purchased from TransGen Biotech, anti-Flag antibody (20543–1-AP, CL594–66008), anti-Myc antibody (16286–1-AP, CL488–60003), anti-GFP polyclonal antibody (50430–2-AP), anti-HA antibody (51064–2-AP, CL647–81290) were purchased from Proteintech, anti-PRRSV-2 N monoclonal antibody was prepared by our laboratory; Lipofectamine RNAiMAX Transfection Reagent (13778150), Lipofectamine 3000 Transfection Reagent (L3000015) and LysoTracker Red DND-99 (L7528) were purchased from Thermo Fisher Scientific; CFDA (S1076) was purchased from Solarbio; YF 488-Phalloidin (YP0114L) was purchased from UElandy; Latrunculin A (HY-16929), 3 × Enzyme-linked Immunosorbent Assay:Article Title: PRRSV-2 impedes lysosomes from eliminating secondary infected bacteria. Article Snippet: The co-localization of Smurf1, RhoA and nsp5 was analyzed using ImageJ, and the results were quantified using the Pearson’s correlation coefficient. n = 10 cells from three independent experiments. (E) HEK-293T cells were co-transfected with HA-nsp5, Flag- Smurf1 and MycRhoA plasmids for 36 h, the cells were harvested to detect the interaction between nsp5, RhoA and Smurf1 by ternary Co-IP. (F) HEK-293T cells were co-transfected with the plasmids of RhoA, Smurf1 and Ub for 36 h, the cells were harvested to detect the ubiquitination of RhoA induced by Smurf1. (G) HEK-293T cells were co-transfected with the plasmids of RhoA, Smurf1, nsp5 and Ub for 36 h, the cells were harvested to detect the effect of nsp5 on Smurf1-induced polyubiquitination of RhoA. (H) HEK-293T cells were co-transfected with the plasmids of RhoA, Smurf1, nsp5 and K63-Ub for 36 h, the cells were harvested to detect the effect of nsp5 on Smurf1-induced polyubiquitination of RhoA. (I) HEK-293T cells were co-transfected with the plasmids of RhoA, nsp5 and Smurf1 for 36 h, the cells were harvested to detect the RhoA, nsp5 and Smurf1 protein expression by Western blot, tubulin served as an internal control. (J) Smurf1+/+ and Smurf1-/- HEK-293T cells were co-transfected with the plasmids of RhoA and nsp5 for 36 h, the cells were harvested to detect the RhoA, nsp5 and Smurf1 protein expression by Western blot, tubulin served as an internal control. (K) Smurf1-/- HEK-293T cells were co-transfected with the plasmids of RhoA, nsp5 and Smurf1 for 36 h, the cells were harvested to detect the RhoA, nsp5 and Smurf1 protein expression by Western blot, tubulin served as an internal control. (L) PAMs were transfected with siNC and siSmurf1 for 24 h, and then infected with recombinant lentivirus expressing nsp5 or control lentivirus for 24 h, the cells were harvested to detect the RhoA, nsp5 and Smurf1 protein expression by Western blot, tubulin served as an internal control. .. Scale bar: 10 μm. https://doi.org/10.1371/journal.ppat.1014000.g008 PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1014000 February 27, 2026 20 / 27 https://doi.org/10.1371/journal.ppat.1014000.g009 PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1014000 February 27, 2026 21 / 27 were purchased from Proteintech, anti-PRRSV-2 N monoclonal antibody was prepared by our laboratory; Lipofectamine RNAiMAX Transfection Reagent (13778150), Lipofectamine 3000 Transfection Reagent (L3000015) and LysoTracker Red DND-99 (L7528) were purchased from Thermo Fisher Scientific; CFDA (S1076) was purchased from Solarbio; YF 488-Phalloidin (YP0114L) was purchased from UElandy; Latrunculin A (HY-16929), 3 × Article Title: PRRSV-2 impedes lysosomes from eliminating secondary infected bacteria Article Snippet: .. Anti-RhoA monoclonal antibody (#2117) was purchased from Cell Signaling Technology, Anti-Smurf1 (sc-100616) was purchased from Santa Cruz Biotechnology, anti-RhoA antibody (10749–1-AP, 66733–1-Ig), anti-ATG7 polyclonal antibody (10088–2-AP), anti-TOLLIP polyclonal antibody (11315–1-AP), anti-LAMP1 monoclonal antibody (CL647–65051), anti-ubiquitin Polyclonal antibody (80992–1-RR) were purchased from Proteintech, anti-tubulin monoclonal antibody (HC-101) was purchased from TransGen Biotech, anti-Flag antibody (20543–1-AP, CL594–66008), anti-Myc antibody (16286–1-AP, CL488–60003), anti-GFP polyclonal antibody (50430–2-AP), anti-HA antibody (51064–2-AP, CL647–81290) were purchased from Proteintech, anti-PRRSV-2 N monoclonal antibody was prepared by our laboratory; Lipofectamine RNAiMAX Transfection Reagent (13778150), Lipofectamine 3000 Transfection Reagent (L3000015) and LysoTracker Red DND-99 (L7528) were purchased from Thermo Fisher Scientific; CFDA (S1076) was purchased from Solarbio; YF 488-Phalloidin (YP0114L) was purchased from UElandy; Latrunculin A (HY-16929), 3 × Purification:Article Title: PIP2 Binding at Allosteric Site Blocks Activation in Human Rod CNG Channels Article Snippet: For protein production, HEK293F cells (Thermo Fisher Scientific) were infected at a virus-to-cell ratio of 1:10 (v/v) and supplemented with 10 mM sodium butyrate 16 h after the baculovirus infection, followed by 48 h incubation at 37 °C. .. Harvested cells were resuspended in purification buffer (25 mM HEPES, 150 mM KCl, pH 7.4) containing 50 μM cGMP and a tablet of protease inhibitors cocktail, cOmplete (Roche), and membranes were solubilized in 1% (w/v) lauryl maltose neopentyl glycol (LMNG) (Anatrace) for 1.5 h. After centrifugation, the supernatant was incubated with anti-DYKDDDDK G1 affinity resin (Genscript) for 1 h, washed with the purification buffer containing 0.06% glyco-diosgenin (GDN) (Anatrace) and 50 μM cGMP, and eluted with 0.15 mg/ml Centrifugation:Article Title: PIP2 Binding at Allosteric Site Blocks Activation in Human Rod CNG Channels Article Snippet: For protein production, HEK293F cells (Thermo Fisher Scientific) were infected at a virus-to-cell ratio of 1:10 (v/v) and supplemented with 10 mM sodium butyrate 16 h after the baculovirus infection, followed by 48 h incubation at 37 °C. .. Harvested cells were resuspended in purification buffer (25 mM HEPES, 150 mM KCl, pH 7.4) containing 50 μM cGMP and a tablet of protease inhibitors cocktail, cOmplete (Roche), and membranes were solubilized in 1% (w/v) lauryl maltose neopentyl glycol (LMNG) (Anatrace) for 1.5 h. After centrifugation, the supernatant was incubated with anti-DYKDDDDK G1 affinity resin (Genscript) for 1 h, washed with the purification buffer containing 0.06% glyco-diosgenin (GDN) (Anatrace) and 50 μM cGMP, and eluted with 0.15 mg/ml Incubation:Article Title: PIP2 Binding at Allosteric Site Blocks Activation in Human Rod CNG Channels Article Snippet: For protein production, HEK293F cells (Thermo Fisher Scientific) were infected at a virus-to-cell ratio of 1:10 (v/v) and supplemented with 10 mM sodium butyrate 16 h after the baculovirus infection, followed by 48 h incubation at 37 °C. .. Harvested cells were resuspended in purification buffer (25 mM HEPES, 150 mM KCl, pH 7.4) containing 50 μM cGMP and a tablet of protease inhibitors cocktail, cOmplete (Roche), and membranes were solubilized in 1% (w/v) lauryl maltose neopentyl glycol (LMNG) (Anatrace) for 1.5 h. After centrifugation, the supernatant was incubated with anti-DYKDDDDK G1 affinity resin (Genscript) for 1 h, washed with the purification buffer containing 0.06% glyco-diosgenin (GDN) (Anatrace) and 50 μM cGMP, and eluted with 0.15 mg/ml |