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ltl fitc  (Vector Laboratories)


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    Structured Review

    Vector Laboratories ltl fitc
    Ltl Fitc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 410 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fitc+ltl/Biotinylated+Lotus+Tetragonolobus+Lectin+(LTL)/pm41898549-239-31-32
    Average 96 stars, based on 410 article reviews
    ltl fitc - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: CUB domain-containing protein 1 controls HGF responses by integrating Src and Met–STAT3 signaling
    Article Snippet: The fixed kidneys were embedded in OCT compound (Sakura Finetek), sectioned, and mounted on glass slides. .. The kidney sections were blocked with Blocking One (Nacalai Tesque), incubated with primary antibodies, and then incubated with Alexa Fluor 488/594-conjugated secondary antibodies (Molecular Probes) and the fluorescein-conjugated proximal tubule marker, FITC-LTL (FL-1321, Vector Laboratories). ..

    Article Title: CDCP1 promotes compensatory renal growth by integrating Src and Met signaling
    Article Snippet: The fixed kidneys were embedded in OCT compound (Sakura Finetek), sectioned, and mounted on glass slides. .. The kidney sections were blocked with Blocking One (Nacalai Tesque), incubated with primary antibodies for overnight at 4°C, and then incubated with Alexa Fluor 488/594–conjugated secondary antibodies (Molecular Probes) and the fluorescein-conjugated proximal tubule marker, FITC-LTL (FL-1321; Vector Laboratories), for 2 h at room temperature. ..

    Incubation:

    Article Title: CUB domain-containing protein 1 controls HGF responses by integrating Src and Met–STAT3 signaling
    Article Snippet: The fixed kidneys were embedded in OCT compound (Sakura Finetek), sectioned, and mounted on glass slides. .. The kidney sections were blocked with Blocking One (Nacalai Tesque), incubated with primary antibodies, and then incubated with Alexa Fluor 488/594-conjugated secondary antibodies (Molecular Probes) and the fluorescein-conjugated proximal tubule marker, FITC-LTL (FL-1321, Vector Laboratories). ..

    Article Title: Tubular cells produce FGF2 via autophagy after acute kidney injury leading to fibroblast activation and renal fibrosis.
    Article Snippet: Following acute kidney injury (AKI), renal tubular cells may stimulate fibroblasts in a paracrine fashion leading to interstitial fibrosis, but the paracrine factors and their regulation under this condition remain elusive.. Here we identify a macroautophagy/autophagy-dependent FGF2 (fibroblast growth factor 2) production in tubular cells.. Upon induction, FGF2 acts as a key paracrine factor to activate fibroblasts for renal fibrosis.

    Article Title: Noncanonical function of Pannexin1 promotes cellular senescence and renal fibrosis post-acute kidney injury
    Article Snippet: The staining mixture from RiboBio (China) was used to stain the cells for 30 min. .. For the identification of other renal cell types, the sections were incubated overnight at 4 °C with FITC-LTL (Vector Laboratories, FL-1321-2) and primary antibodies against F4/80 (1:50, Cell Signaling Technology, 70076S), αSMA (1:50, Proteintech, 14395-1-AP), or CD31 (1:50, Cell Signaling Technology, 77699 s). ..

    Article Title: CDCP1 promotes compensatory renal growth by integrating Src and Met signaling
    Article Snippet: The fixed kidneys were embedded in OCT compound (Sakura Finetek), sectioned, and mounted on glass slides. .. The kidney sections were blocked with Blocking One (Nacalai Tesque), incubated with primary antibodies for overnight at 4°C, and then incubated with Alexa Fluor 488/594–conjugated secondary antibodies (Molecular Probes) and the fluorescein-conjugated proximal tubule marker, FITC-LTL (FL-1321; Vector Laboratories), for 2 h at room temperature. ..

    Marker:

    Article Title: CUB domain-containing protein 1 controls HGF responses by integrating Src and Met–STAT3 signaling
    Article Snippet: The fixed kidneys were embedded in OCT compound (Sakura Finetek), sectioned, and mounted on glass slides. .. The kidney sections were blocked with Blocking One (Nacalai Tesque), incubated with primary antibodies, and then incubated with Alexa Fluor 488/594-conjugated secondary antibodies (Molecular Probes) and the fluorescein-conjugated proximal tubule marker, FITC-LTL (FL-1321, Vector Laboratories). ..

    Article Title: CDCP1 promotes compensatory renal growth by integrating Src and Met signaling
    Article Snippet: The fixed kidneys were embedded in OCT compound (Sakura Finetek), sectioned, and mounted on glass slides. .. The kidney sections were blocked with Blocking One (Nacalai Tesque), incubated with primary antibodies for overnight at 4°C, and then incubated with Alexa Fluor 488/594–conjugated secondary antibodies (Molecular Probes) and the fluorescein-conjugated proximal tubule marker, FITC-LTL (FL-1321; Vector Laboratories), for 2 h at room temperature. ..

    Staining:

    Article Title: Tubular cells produce FGF2 via autophagy after acute kidney injury leading to fibroblast activation and renal fibrosis.
    Article Snippet: Following acute kidney injury (AKI), renal tubular cells may stimulate fibroblasts in a paracrine fashion leading to interstitial fibrosis, but the paracrine factors and their regulation under this condition remain elusive.. Here we identify a macroautophagy/autophagy-dependent FGF2 (fibroblast growth factor 2) production in tubular cells.. Upon induction, FGF2 acts as a key paracrine factor to activate fibroblasts for renal fibrosis.

    other:

    Article Title: Noncanonical function of Pannexin1 promotes cellular senescence and renal fibrosis post-acute kidney injury.
    Article Snippet: The visualization of antibody labeling on the membranes was accomplished via the Immobile Western Chemiluminescence HRP substrate kit (Millipore).



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    Vector Laboratories fitc conjugated ltl
    (A) Intravital two-photon (2P) microscopy was used to quantify single-nephron tubular fluid flow dynamics in the kidney cortex. Representative time-lapse images with Lucifer Yellow (LY) or 70kDa dextran-rhodamine show real-time changes in tubular fluid flow dynamics in the kidney of an anaesthetized 3-month-old male C57BL/6J mouse. 2M dextran-rhodamine was used together with LY to label the circulating plasma. (B) A representative recording of 70kDa dextran-rhodamine fluorescence intensity within a luminal ROI of the early PT segment in an anaesthetized 3-month-old male C57BL/6J mouse. Raw signals were overlaid with a reference sine wave of fixed frequency as indicated. (C) Resting-state magnetic resonance imaging (MRI) was used to detect physiological oscillations at the multi-nephron spatial scale. With a resolution of 0.5×0.5×0.5mm 3 in a rat kidney, single-voxel recordings indicate collective fluid dynamics among about five adjacent nephrons in the renal cortex . The plot on the right represents a single-voxel time series obtained from a cortical region of an anaesthetized 3-month-old male Sprague-Dawley rat. Raw signals were overlaid with a reference sine wave. (D) Transit-time ultrasonic flow measurement was used to measure real-time renal blood flow through the renal artery. Data shown was collected from an anaesthetized 6-month-old male C57BL/6J mouse, previously reported in ref. . The original 1000Hz data was down-sampled to 2Hz and bandpass-filtered to visualize slow rhythms below 0.1 Hz, overlaid with a reference sine wave. (E) Glomerular filtration rate (GFR) can be estimated by fitting the transcutaneous clearance profile of a single intravenous bolus of <t>FITC-sinistrin.</t> Data shown was collected from an anaesthetized 3-month-old male C57BL/6J mouse, previously reported in ref. . The raw data points, with a 0.5 Hz sampling rate, are colored in red. Detrended signals for a 200-second interval are shown on the right, overlaid with a reference sine wave.
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    Vector Laboratories fluorescein labeled lotus tetragonolobus lectin (ltl)
    (A) Intravital two-photon (2P) microscopy was used to quantify single-nephron tubular fluid flow dynamics in the kidney cortex. Representative time-lapse images with Lucifer Yellow (LY) or 70kDa dextran-rhodamine show real-time changes in tubular fluid flow dynamics in the kidney of an anaesthetized 3-month-old male C57BL/6J mouse. 2M dextran-rhodamine was used together with LY to label the circulating plasma. (B) A representative recording of 70kDa dextran-rhodamine fluorescence intensity within a luminal ROI of the early PT segment in an anaesthetized 3-month-old male C57BL/6J mouse. Raw signals were overlaid with a reference sine wave of fixed frequency as indicated. (C) Resting-state magnetic resonance imaging (MRI) was used to detect physiological oscillations at the multi-nephron spatial scale. With a resolution of 0.5×0.5×0.5mm 3 in a rat kidney, single-voxel recordings indicate collective fluid dynamics among about five adjacent nephrons in the renal cortex . The plot on the right represents a single-voxel time series obtained from a cortical region of an anaesthetized 3-month-old male Sprague-Dawley rat. Raw signals were overlaid with a reference sine wave. (D) Transit-time ultrasonic flow measurement was used to measure real-time renal blood flow through the renal artery. Data shown was collected from an anaesthetized 6-month-old male C57BL/6J mouse, previously reported in ref. . The original 1000Hz data was down-sampled to 2Hz and bandpass-filtered to visualize slow rhythms below 0.1 Hz, overlaid with a reference sine wave. (E) Glomerular filtration rate (GFR) can be estimated by fitting the transcutaneous clearance profile of a single intravenous bolus of <t>FITC-sinistrin.</t> Data shown was collected from an anaesthetized 3-month-old male C57BL/6J mouse, previously reported in ref. . The raw data points, with a 0.5 Hz sampling rate, are colored in red. Detrended signals for a 200-second interval are shown on the right, overlaid with a reference sine wave.
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    Image Search Results


    (A) Intravital two-photon (2P) microscopy was used to quantify single-nephron tubular fluid flow dynamics in the kidney cortex. Representative time-lapse images with Lucifer Yellow (LY) or 70kDa dextran-rhodamine show real-time changes in tubular fluid flow dynamics in the kidney of an anaesthetized 3-month-old male C57BL/6J mouse. 2M dextran-rhodamine was used together with LY to label the circulating plasma. (B) A representative recording of 70kDa dextran-rhodamine fluorescence intensity within a luminal ROI of the early PT segment in an anaesthetized 3-month-old male C57BL/6J mouse. Raw signals were overlaid with a reference sine wave of fixed frequency as indicated. (C) Resting-state magnetic resonance imaging (MRI) was used to detect physiological oscillations at the multi-nephron spatial scale. With a resolution of 0.5×0.5×0.5mm 3 in a rat kidney, single-voxel recordings indicate collective fluid dynamics among about five adjacent nephrons in the renal cortex . The plot on the right represents a single-voxel time series obtained from a cortical region of an anaesthetized 3-month-old male Sprague-Dawley rat. Raw signals were overlaid with a reference sine wave. (D) Transit-time ultrasonic flow measurement was used to measure real-time renal blood flow through the renal artery. Data shown was collected from an anaesthetized 6-month-old male C57BL/6J mouse, previously reported in ref. . The original 1000Hz data was down-sampled to 2Hz and bandpass-filtered to visualize slow rhythms below 0.1 Hz, overlaid with a reference sine wave. (E) Glomerular filtration rate (GFR) can be estimated by fitting the transcutaneous clearance profile of a single intravenous bolus of FITC-sinistrin. Data shown was collected from an anaesthetized 3-month-old male C57BL/6J mouse, previously reported in ref. . The raw data points, with a 0.5 Hz sampling rate, are colored in red. Detrended signals for a 200-second interval are shown on the right, overlaid with a reference sine wave.

    Journal: bioRxiv

    Article Title: Life in the fast lane: Functional consequences of male-female dynamic differences in the renal autoregulation of flow

    doi: 10.1101/2025.09.12.675896

    Figure Lengend Snippet: (A) Intravital two-photon (2P) microscopy was used to quantify single-nephron tubular fluid flow dynamics in the kidney cortex. Representative time-lapse images with Lucifer Yellow (LY) or 70kDa dextran-rhodamine show real-time changes in tubular fluid flow dynamics in the kidney of an anaesthetized 3-month-old male C57BL/6J mouse. 2M dextran-rhodamine was used together with LY to label the circulating plasma. (B) A representative recording of 70kDa dextran-rhodamine fluorescence intensity within a luminal ROI of the early PT segment in an anaesthetized 3-month-old male C57BL/6J mouse. Raw signals were overlaid with a reference sine wave of fixed frequency as indicated. (C) Resting-state magnetic resonance imaging (MRI) was used to detect physiological oscillations at the multi-nephron spatial scale. With a resolution of 0.5×0.5×0.5mm 3 in a rat kidney, single-voxel recordings indicate collective fluid dynamics among about five adjacent nephrons in the renal cortex . The plot on the right represents a single-voxel time series obtained from a cortical region of an anaesthetized 3-month-old male Sprague-Dawley rat. Raw signals were overlaid with a reference sine wave. (D) Transit-time ultrasonic flow measurement was used to measure real-time renal blood flow through the renal artery. Data shown was collected from an anaesthetized 6-month-old male C57BL/6J mouse, previously reported in ref. . The original 1000Hz data was down-sampled to 2Hz and bandpass-filtered to visualize slow rhythms below 0.1 Hz, overlaid with a reference sine wave. (E) Glomerular filtration rate (GFR) can be estimated by fitting the transcutaneous clearance profile of a single intravenous bolus of FITC-sinistrin. Data shown was collected from an anaesthetized 3-month-old male C57BL/6J mouse, previously reported in ref. . The raw data points, with a 0.5 Hz sampling rate, are colored in red. Detrended signals for a 200-second interval are shown on the right, overlaid with a reference sine wave.

    Article Snippet: The following antibodies were employed in this study recognizing KIM-1 (kidney injury molecule-1, Rat, 1:500, R&D Systems, MAB1817), FITC-conjugated LTL (Lotis Tetragonolobus Lectin, 1:1000, Vector Laboratories, FL-1321).

    Techniques: Microscopy, Clinical Proteomics, Fluorescence, Magnetic Resonance Imaging, Filtration, Sampling