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fish leptin  (Cusabio)


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    Cusabio fish leptin
    Fish Leptin, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fish+leptin/Fish+(Salmon)+leptin+(LEP)+ELISA+kit/pmc12657820-114-14-18
    Average 93 stars, based on 5 article reviews
    fish leptin - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Epigallocatechin-3-gallate supplementation regulates glucose redistribution through its potential endogenous receptor in grass carp ( Ctenopharyngodon idella )
    Article Snippet: The activities of α-amylase and α-glycosidase in the intestine were determined by commercial kits (cat. #BC0610 and BC2550, Beijing Solarbio Science & Technology Co., Ltd.). .. The concentration of leptin in plasma was measured using an ELISA kit designed for fish leptin (cat. #CSB-EL012870FI, Cusabio Biotech Co., Ltd., Wuhan, China) ( ). .. The L8824 grass carp hepatocyte line (China Center for Type Culture Collection [CCTCC], Wuhan, China) was cultured in Modified Eagle's Medium (containing 1000.0 mg/L D-glucose) (HyClone Laboratories, Logan, UT, USA) with 10% fetal bovine serum (FBS; Invitrogen, Carlsbad, CA, USA), 100 IU/mL penicillin, and 100 μg/mL streptomycin at 28 °C in a 5% CO 2 -humidified atmosphere.

    Clinical Proteomics:

    Article Title: Epigallocatechin-3-gallate supplementation regulates glucose redistribution through its potential endogenous receptor in grass carp ( Ctenopharyngodon idella )
    Article Snippet: The activities of α-amylase and α-glycosidase in the intestine were determined by commercial kits (cat. #BC0610 and BC2550, Beijing Solarbio Science & Technology Co., Ltd.). .. The concentration of leptin in plasma was measured using an ELISA kit designed for fish leptin (cat. #CSB-EL012870FI, Cusabio Biotech Co., Ltd., Wuhan, China) ( ). .. The L8824 grass carp hepatocyte line (China Center for Type Culture Collection [CCTCC], Wuhan, China) was cultured in Modified Eagle's Medium (containing 1000.0 mg/L D-glucose) (HyClone Laboratories, Logan, UT, USA) with 10% fetal bovine serum (FBS; Invitrogen, Carlsbad, CA, USA), 100 IU/mL penicillin, and 100 μg/mL streptomycin at 28 °C in a 5% CO 2 -humidified atmosphere.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Epigallocatechin-3-gallate supplementation regulates glucose redistribution through its potential endogenous receptor in grass carp ( Ctenopharyngodon idella )
    Article Snippet: The activities of α-amylase and α-glycosidase in the intestine were determined by commercial kits (cat. #BC0610 and BC2550, Beijing Solarbio Science & Technology Co., Ltd.). .. The concentration of leptin in plasma was measured using an ELISA kit designed for fish leptin (cat. #CSB-EL012870FI, Cusabio Biotech Co., Ltd., Wuhan, China) ( ). .. The L8824 grass carp hepatocyte line (China Center for Type Culture Collection [CCTCC], Wuhan, China) was cultured in Modified Eagle's Medium (containing 1000.0 mg/L D-glucose) (HyClone Laboratories, Logan, UT, USA) with 10% fetal bovine serum (FBS; Invitrogen, Carlsbad, CA, USA), 100 IU/mL penicillin, and 100 μg/mL streptomycin at 28 °C in a 5% CO 2 -humidified atmosphere.



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    Expression levels of mxd3 regulate somatic growth and obesity in gMX2 adults. ( A ) Macroscopic view of male and female gMX2 adults compared with WT controls at 10 mpf. ( B ) Growth curves of zebrafish from juvenile to adult stages in two discrete groups (WT ± Dox, gMX2 ± Dox, and hMX3 ± Dox; n = 24/group). ( C ) Bar chart displaying the BMI index in two discrete groups ( n = 25/group) at 3 and 10 mpf stages. ( D ) Viscera and visceral fat pads of gMX2 groups at 12 mpf, displaying ( E ) enlarged internal organs, and ( F ) fat pad size. ( G , H ) Histological characteristics of adipose tissue in H&E-stained sections displaying ( G ) male and ( H ) female samples. Left: H&E-stained sections displaying adipose tissues of WT ± Dox and gMX2 ± Dox male groups ( n = 6/group). Right: morphometric analysis of fat on adipose tissues of average size in each group ( n = 6/group). ( I ) Molecular analysis of visceral adipose tissue of gMX2 and hMX3 groups revealed upregulation of mature adipocyte markers genes, such as ppar-γ, fabp4, scd1, adipoq, and cav1 . ( J ) Blood analysis of leptin and adiponectin. Statistically significant differences from the controls are indicated by * ( p < 0.05), and ** ( p < 0.01).

    Journal: Cells

    Article Title: MXD3 Promotes Obesity and the Androgen Receptor Signaling Pathway in Gender-Disparity Hepatocarcinogenesis

    doi: 10.3390/cells10123434

    Figure Lengend Snippet: Expression levels of mxd3 regulate somatic growth and obesity in gMX2 adults. ( A ) Macroscopic view of male and female gMX2 adults compared with WT controls at 10 mpf. ( B ) Growth curves of zebrafish from juvenile to adult stages in two discrete groups (WT ± Dox, gMX2 ± Dox, and hMX3 ± Dox; n = 24/group). ( C ) Bar chart displaying the BMI index in two discrete groups ( n = 25/group) at 3 and 10 mpf stages. ( D ) Viscera and visceral fat pads of gMX2 groups at 12 mpf, displaying ( E ) enlarged internal organs, and ( F ) fat pad size. ( G , H ) Histological characteristics of adipose tissue in H&E-stained sections displaying ( G ) male and ( H ) female samples. Left: H&E-stained sections displaying adipose tissues of WT ± Dox and gMX2 ± Dox male groups ( n = 6/group). Right: morphometric analysis of fat on adipose tissues of average size in each group ( n = 6/group). ( I ) Molecular analysis of visceral adipose tissue of gMX2 and hMX3 groups revealed upregulation of mature adipocyte markers genes, such as ppar-γ, fabp4, scd1, adipoq, and cav1 . ( J ) Blood analysis of leptin and adiponectin. Statistically significant differences from the controls are indicated by * ( p < 0.05), and ** ( p < 0.01).

    Article Snippet: The collected blood samples were pooled and used for quantification of leptin (Fish Leptin ELISA Kit, MBS021271, MyBioSource, San Diego, CA, USA) and adiponectin (Fish Adiponectin ELISA Kit, MBS098337, MyBioSource San Diego, CA, USA) by ELISA, and followed the manufacturer’s instructions.

    Techniques: Expressing, Staining