pvdf membrane (Bio-Rad)
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pvdf membrane
Pvdf Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/filter+paper+blot/Immun-Blot+PVDF%2FFilter+Paper+Sandwiches/bio_rxiv__64898__2026__05__04__722637-378-14-16
Average 94 stars, based on 105 article reviews
Pvdf Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/filter+paper+blot/Immun-Blot+PVDF%2FFilter+Paper+Sandwiches/bio_rxiv__64898__2026__05__04__722637-378-14-16
Average 94 stars, based on 105 article reviews
pvdf membrane - by Bioz Stars,
2026-09
94/100 stars
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Electrophoresis:Article Title: Analysis of Reactive Oxygen Species-Induced Cellular Damage in Cervical Cancer. Article Snippet: Materials 1× PBS (see recipe) Mammalian Protein Extraction Reagent (M-PER, Thermo Fisher Scientific, cat. no. 78501) 100× Halt Protease Inhibitor Cocktail, EDTA-free (Thermo Fisher Scientific, cat. no. 87786) BCA protein assay kit (Pierce, Thermo Fisher Scientific, cat. no. 23227) NuPage 4%-12% Bis-Tris gel, 1.0 mm, 12 well (Thermo Fisher Scientific, cat. no. NP0322BOX) NuPage 4× LDS sample buffer (Thermo Fisher Scientific, cat. no. NP0008) Running buffer (see recipe) SDS-PAGE standard (dual stain, Lasec, cat. no. BBRD1610374) Methanol, >99.8%, ACS reagent (Sigma-Aldrich, cat. no. 179337) Transfer buffer (see recipe) Ponceau S stain (Thermo Fisher Scientific, cat. no. A40000279) Blocking buffer (see recipe) PBS-Tween: 1× PBS with .2% (v/v) Tween 20 (Sigma-Aldrich, cat no. P1379) AKT polyclonal antibody (Proteintech, cat. no. PTE 10176-2-AP) HRP-conjugated goat anti-rabbit IgG(H+L) antibody (Proteintech, cat. no. PTE SA00001-2) Pierce ECL western blotting substrate (Thermo Fisher Scientific, cat. no. 32209) HRP-conjugated mouse anti-β-actin antibody (Sigma-Aldrich, cat. no. A3854) Mild stripping buffer (see recipe) Chan et al. 14 of 30 Current Protocols 26911299, 2026, 3, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70328 by G uim arães M arina B orges - U N B - U niversidade de B rasilia , W iley O nline L ibrary on [21/03/2026]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Cell scraper (Sigma-Aldrich, cat. no. CLS353085) 1.5-ml microcentrifuge tubes (Separations, cat. no. VWRA525-1528) 96-well tissue culture plates, clear, flat-bottom, polystyrene (Lasec, cat. no. P1PLA044C-000096ST) Powerpac 200 Electrophoresis power supply (Bio-Rad, cat. no. 165-5052, 165-5053) ELx800 Universal Microplate Reader (Bio-Tek Instruments) 94°C heating block (Laboratory Equipment Supplies, cat. no. ABH1) Invitrogen XCell SureLock Mini-Cell (Thermo Fisher Scientific, cat. no. EI0001) Scienceware polypropylene tray, 30 cm × 20 cm (Sigma-Aldrich, cat. no. Z177814) Transfer apparatus (Mini-PROTEAN Vertical Electrophoresis Cell, 4-gel, with Mini Trans-Blot Module, Bio-Rad, cat. no. 1658030) Blocking Assay:Article Title: Analysis of Reactive Oxygen Species-Induced Cellular Damage in Cervical Cancer. Article Snippet: Materials 1× PBS (see recipe) Mammalian Protein Extraction Reagent (M-PER, Thermo Fisher Scientific, cat. no. 78501) 100× Halt Protease Inhibitor Cocktail, EDTA-free (Thermo Fisher Scientific, cat. no. 87786) BCA protein assay kit (Pierce, Thermo Fisher Scientific, cat. no. 23227) NuPage 4%-12% Bis-Tris gel, 1.0 mm, 12 well (Thermo Fisher Scientific, cat. no. NP0322BOX) NuPage 4× LDS sample buffer (Thermo Fisher Scientific, cat. no. NP0008) Running buffer (see recipe) SDS-PAGE standard (dual stain, Lasec, cat. no. BBRD1610374) Methanol, >99.8%, ACS reagent (Sigma-Aldrich, cat. no. 179337) Transfer buffer (see recipe) Ponceau S stain (Thermo Fisher Scientific, cat. no. A40000279) Blocking buffer (see recipe) PBS-Tween: 1× PBS with .2% (v/v) Tween 20 (Sigma-Aldrich, cat no. P1379) AKT polyclonal antibody (Proteintech, cat. no. PTE 10176-2-AP) HRP-conjugated goat anti-rabbit IgG(H+L) antibody (Proteintech, cat. no. PTE SA00001-2) Pierce ECL western blotting substrate (Thermo Fisher Scientific, cat. no. 32209) HRP-conjugated mouse anti-β-actin antibody (Sigma-Aldrich, cat. no. A3854) Mild stripping buffer (see recipe) Chan et al. 14 of 30 Current Protocols 26911299, 2026, 3, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70328 by G uim arães M arina B orges - U N B - U niversidade de B rasilia , W iley O nline L ibrary on [21/03/2026]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Cell scraper (Sigma-Aldrich, cat. no. CLS353085) 1.5-ml microcentrifuge tubes (Separations, cat. no. VWRA525-1528) 96-well tissue culture plates, clear, flat-bottom, polystyrene (Lasec, cat. no. P1PLA044C-000096ST) Powerpac 200 Electrophoresis power supply (Bio-Rad, cat. no. 165-5052, 165-5053) ELx800 Universal Microplate Reader (Bio-Tek Instruments) 94°C heating block (Laboratory Equipment Supplies, cat. no. ABH1) Invitrogen XCell SureLock Mini-Cell (Thermo Fisher Scientific, cat. no. EI0001) Scienceware polypropylene tray, 30 cm × 20 cm (Sigma-Aldrich, cat. no. Z177814) Transfer apparatus (Mini-PROTEAN Vertical Electrophoresis Cell, 4-gel, with Mini Trans-Blot Module, Bio-Rad, cat. no. 1658030) Western Blot:Article Title: Analysis of Reactive Oxygen Species-Induced Cellular Damage in Cervical Cancer. Article Snippet: Materials 1× PBS (see recipe) Mammalian Protein Extraction Reagent (M-PER, Thermo Fisher Scientific, cat. no. 78501) 100× Halt Protease Inhibitor Cocktail, EDTA-free (Thermo Fisher Scientific, cat. no. 87786) BCA protein assay kit (Pierce, Thermo Fisher Scientific, cat. no. 23227) NuPage 4%-12% Bis-Tris gel, 1.0 mm, 12 well (Thermo Fisher Scientific, cat. no. NP0322BOX) NuPage 4× LDS sample buffer (Thermo Fisher Scientific, cat. no. NP0008) Running buffer (see recipe) SDS-PAGE standard (dual stain, Lasec, cat. no. BBRD1610374) Methanol, >99.8%, ACS reagent (Sigma-Aldrich, cat. no. 179337) Transfer buffer (see recipe) Ponceau S stain (Thermo Fisher Scientific, cat. no. A40000279) Blocking buffer (see recipe) PBS-Tween: 1× PBS with .2% (v/v) Tween 20 (Sigma-Aldrich, cat no. P1379) AKT polyclonal antibody (Proteintech, cat. no. PTE 10176-2-AP) HRP-conjugated goat anti-rabbit IgG(H+L) antibody (Proteintech, cat. no. PTE SA00001-2) Pierce ECL western blotting substrate (Thermo Fisher Scientific, cat. no. 32209) HRP-conjugated mouse anti-β-actin antibody (Sigma-Aldrich, cat. no. A3854) Mild stripping buffer (see recipe) Chan et al. 14 of 30 Current Protocols 26911299, 2026, 3, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70328 by G uim arães M arina B orges - U N B - U niversidade de B rasilia , W iley O nline L ibrary on [21/03/2026]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Cell scraper (Sigma-Aldrich, cat. no. CLS353085) 1.5-ml microcentrifuge tubes (Separations, cat. no. VWRA525-1528) 96-well tissue culture plates, clear, flat-bottom, polystyrene (Lasec, cat. no. P1PLA044C-000096ST) Powerpac 200 Electrophoresis power supply (Bio-Rad, cat. no. 165-5052, 165-5053) ELx800 Universal Microplate Reader (Bio-Tek Instruments) 94°C heating block (Laboratory Equipment Supplies, cat. no. ABH1) Invitrogen XCell SureLock Mini-Cell (Thermo Fisher Scientific, cat. no. EI0001) Scienceware polypropylene tray, 30 cm × 20 cm (Sigma-Aldrich, cat. no. Z177814) Transfer apparatus (Mini-PROTEAN Vertical Electrophoresis Cell, 4-gel, with Mini Trans-Blot Module, Bio-Rad, cat. no. 1658030) Membrane:Article Title: Analysis of Reactive Oxygen Species-Induced Cellular Damage in Cervical Cancer. Article Snippet: Materials 1× PBS (see recipe) Mammalian Protein Extraction Reagent (M-PER, Thermo Fisher Scientific, cat. no. 78501) 100× Halt Protease Inhibitor Cocktail, EDTA-free (Thermo Fisher Scientific, cat. no. 87786) BCA protein assay kit (Pierce, Thermo Fisher Scientific, cat. no. 23227) NuPage 4%-12% Bis-Tris gel, 1.0 mm, 12 well (Thermo Fisher Scientific, cat. no. NP0322BOX) NuPage 4× LDS sample buffer (Thermo Fisher Scientific, cat. no. NP0008) Running buffer (see recipe) SDS-PAGE standard (dual stain, Lasec, cat. no. BBRD1610374) Methanol, >99.8%, ACS reagent (Sigma-Aldrich, cat. no. 179337) Transfer buffer (see recipe) Ponceau S stain (Thermo Fisher Scientific, cat. no. A40000279) Blocking buffer (see recipe) PBS-Tween: 1× PBS with .2% (v/v) Tween 20 (Sigma-Aldrich, cat no. P1379) AKT polyclonal antibody (Proteintech, cat. no. PTE 10176-2-AP) HRP-conjugated goat anti-rabbit IgG(H+L) antibody (Proteintech, cat. no. PTE SA00001-2) Pierce ECL western blotting substrate (Thermo Fisher Scientific, cat. no. 32209) HRP-conjugated mouse anti-β-actin antibody (Sigma-Aldrich, cat. no. A3854) Mild stripping buffer (see recipe) Chan et al. 14 of 30 Current Protocols 26911299, 2026, 3, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70328 by G uim arães M arina B orges - U N B - U niversidade de B rasilia , W iley O nline L ibrary on [21/03/2026]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Cell scraper (Sigma-Aldrich, cat. no. CLS353085) 1.5-ml microcentrifuge tubes (Separations, cat. no. VWRA525-1528) 96-well tissue culture plates, clear, flat-bottom, polystyrene (Lasec, cat. no. P1PLA044C-000096ST) Powerpac 200 Electrophoresis power supply (Bio-Rad, cat. no. 165-5052, 165-5053) ELx800 Universal Microplate Reader (Bio-Tek Instruments) 94°C heating block (Laboratory Equipment Supplies, cat. no. ABH1) Invitrogen XCell SureLock Mini-Cell (Thermo Fisher Scientific, cat. no. EI0001) Scienceware polypropylene tray, 30 cm × 20 cm (Sigma-Aldrich, cat. no. Z177814) Transfer apparatus (Mini-PROTEAN Vertical Electrophoresis Cell, 4-gel, with Mini Trans-Blot Module, Bio-Rad, cat. no. 1658030) Imaging:Article Title: Analysis of Reactive Oxygen Species-Induced Cellular Damage in Cervical Cancer. Article Snippet: Materials 1× PBS (see recipe) Mammalian Protein Extraction Reagent (M-PER, Thermo Fisher Scientific, cat. no. 78501) 100× Halt Protease Inhibitor Cocktail, EDTA-free (Thermo Fisher Scientific, cat. no. 87786) BCA protein assay kit (Pierce, Thermo Fisher Scientific, cat. no. 23227) NuPage 4%-12% Bis-Tris gel, 1.0 mm, 12 well (Thermo Fisher Scientific, cat. no. NP0322BOX) NuPage 4× LDS sample buffer (Thermo Fisher Scientific, cat. no. NP0008) Running buffer (see recipe) SDS-PAGE standard (dual stain, Lasec, cat. no. BBRD1610374) Methanol, >99.8%, ACS reagent (Sigma-Aldrich, cat. no. 179337) Transfer buffer (see recipe) Ponceau S stain (Thermo Fisher Scientific, cat. no. A40000279) Blocking buffer (see recipe) PBS-Tween: 1× PBS with .2% (v/v) Tween 20 (Sigma-Aldrich, cat no. P1379) AKT polyclonal antibody (Proteintech, cat. no. PTE 10176-2-AP) HRP-conjugated goat anti-rabbit IgG(H+L) antibody (Proteintech, cat. no. PTE SA00001-2) Pierce ECL western blotting substrate (Thermo Fisher Scientific, cat. no. 32209) HRP-conjugated mouse anti-β-actin antibody (Sigma-Aldrich, cat. no. A3854) Mild stripping buffer (see recipe) Chan et al. 14 of 30 Current Protocols 26911299, 2026, 3, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70328 by G uim arães M arina B orges - U N B - U niversidade de B rasilia , W iley O nline L ibrary on [21/03/2026]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Cell scraper (Sigma-Aldrich, cat. no. CLS353085) 1.5-ml microcentrifuge tubes (Separations, cat. no. VWRA525-1528) 96-well tissue culture plates, clear, flat-bottom, polystyrene (Lasec, cat. no. P1PLA044C-000096ST) Powerpac 200 Electrophoresis power supply (Bio-Rad, cat. no. 165-5052, 165-5053) ELx800 Universal Microplate Reader (Bio-Tek Instruments) 94°C heating block (Laboratory Equipment Supplies, cat. no. ABH1) Invitrogen XCell SureLock Mini-Cell (Thermo Fisher Scientific, cat. no. EI0001) Scienceware polypropylene tray, 30 cm × 20 cm (Sigma-Aldrich, cat. no. Z177814) Transfer apparatus (Mini-PROTEAN Vertical Electrophoresis Cell, 4-gel, with Mini Trans-Blot Module, Bio-Rad, cat. no. 1658030) Software:Article Title: Analysis of Reactive Oxygen Species-Induced Cellular Damage in Cervical Cancer. Article Snippet: Materials 1× PBS (see recipe) Mammalian Protein Extraction Reagent (M-PER, Thermo Fisher Scientific, cat. no. 78501) 100× Halt Protease Inhibitor Cocktail, EDTA-free (Thermo Fisher Scientific, cat. no. 87786) BCA protein assay kit (Pierce, Thermo Fisher Scientific, cat. no. 23227) NuPage 4%-12% Bis-Tris gel, 1.0 mm, 12 well (Thermo Fisher Scientific, cat. no. NP0322BOX) NuPage 4× LDS sample buffer (Thermo Fisher Scientific, cat. no. NP0008) Running buffer (see recipe) SDS-PAGE standard (dual stain, Lasec, cat. no. BBRD1610374) Methanol, >99.8%, ACS reagent (Sigma-Aldrich, cat. no. 179337) Transfer buffer (see recipe) Ponceau S stain (Thermo Fisher Scientific, cat. no. A40000279) Blocking buffer (see recipe) PBS-Tween: 1× PBS with .2% (v/v) Tween 20 (Sigma-Aldrich, cat no. P1379) AKT polyclonal antibody (Proteintech, cat. no. PTE 10176-2-AP) HRP-conjugated goat anti-rabbit IgG(H+L) antibody (Proteintech, cat. no. PTE SA00001-2) Pierce ECL western blotting substrate (Thermo Fisher Scientific, cat. no. 32209) HRP-conjugated mouse anti-β-actin antibody (Sigma-Aldrich, cat. no. A3854) Mild stripping buffer (see recipe) Chan et al. 14 of 30 Current Protocols 26911299, 2026, 3, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70328 by G uim arães M arina B orges - U N B - U niversidade de B rasilia , W iley O nline L ibrary on [21/03/2026]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Cell scraper (Sigma-Aldrich, cat. no. CLS353085) 1.5-ml microcentrifuge tubes (Separations, cat. no. VWRA525-1528) 96-well tissue culture plates, clear, flat-bottom, polystyrene (Lasec, cat. no. P1PLA044C-000096ST) Powerpac 200 Electrophoresis power supply (Bio-Rad, cat. no. 165-5052, 165-5053) ELx800 Universal Microplate Reader (Bio-Tek Instruments) 94°C heating block (Laboratory Equipment Supplies, cat. no. ABH1) Invitrogen XCell SureLock Mini-Cell (Thermo Fisher Scientific, cat. no. EI0001) Scienceware polypropylene tray, 30 cm × 20 cm (Sigma-Aldrich, cat. no. Z177814) Transfer apparatus (Mini-PROTEAN Vertical Electrophoresis Cell, 4-gel, with Mini Trans-Blot Module, Bio-Rad, cat. no. 1658030) |