Review



fibroblast growth medium 3  (PromoCell)


Bioz Verified Symbol PromoCell is a verified supplier
Bioz Manufacturer Symbol PromoCell manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    PromoCell fibroblast growth medium 3
    Fibroblast Growth Medium 3, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fibroblast+growth+medium/Fibroblast+Growth+Medium+3/custom%40c-23025%4042681974
    Average 96 stars, based on 99 article reviews
    fibroblast growth medium 3 - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Monoamine Oxidase B (MAO-B) as an Inducer of Mitochondrial Reactive Oxygen Species (ROS) Production and Myofibroblast Differentiation in Cardiac Fibroblasts of Mice
    Article Snippet: .. Pelleted cells were suspended in fibroblast growth medium (PromoCell, Heidelberg, Germany), containing 2% penicillin/streptavidin, plated on 10 cm culture dishes, and incubated in a carbon dioxide (5%) incubator at 37 °C. ..

    Cell Culture:

    Article Title: An Alginate Hydrogel–Lipid Nanodispersion Bio-Mask: A Preliminary Study of Skin Hydration, Barrier Function, and Regenerative Potential
    Article Snippet: The NHDF cell line was purchased from PromoCell. .. Normal fibroblasts were cultured in fibroblast growth medium with a low-serum content (PromoCell, PromoCell GmbH, Heidelberg, Germany) containing a mix of antibiotics-penicillin and streptomycin (1% v / v ; Gibco, Waltham, MA, USA). ..

    Article Title: Antioxidant and Anti-Aging Effects of Porphyra-334 Produced from Saccharomyces cerevisiae in Human Skin Models.
    Article Snippet: .. NHDF (Promocell, Heidelberg, Germany) were cultured in Fibroblast Growth Medium (Promocell) containing supplement mix and 1% penicillin–streptomycin (PS; Gibco, Grand Island, NY, USA). .. HEKa (Gibco) were cultured in Epilife medium (ThermoFisher, Waltham, MA, USA) with Keratinocyte Growth Supplement (HKGS, Gibco) and 1% PS.

    Article Title: Compound design of a patient-derived 3D cell culture system modelling early peritoneal endometriosis
    Article Snippet: NHDFs (C-12300) isolated from the dermis of juvenile foreskin were purchased from Promocell (Heidelberg, Germany). .. NHDFs were cultured in Fibroblast Growth Medium (Promocell, C-23110) containing 1 ng/ml recombinant human basic fibroblast growth factor and 5 μg/ml recombinant human insulin, supplemented with 2 mM L-glutamine and 100 μg/ml Primocin. ..

    Article Title: Sushi, von Willebrand Factor Type A, EGF and Pentraxin Domain‐Containing Protein 1: A Novel Fibroblast‐Derived Circulating Biomarker Reflecting Cardiac Fibrosis
    Article Snippet: .. Human cardiac fibroblasts (HCFs) isolated from the ventricles of adult hearts were purchased from Promocell (#C- 12375) and cultured in fibroblast growth medium (Promocell, #C- 23110) supplemented with 10% fetal bovine serum, penicillin–streptomycin, and insulin at 37 °C and 5% CO2. ..

    Article Title: Synthesis and biological evaluation of 1,3,5-triazine and 2-aminopyrimidine scaffolds as chemical probes targeting the 5-HT 7 -FOXM1 axis in triple-negative breast cancer.
    Article Snippet: a Department of Organic Chemistry and Technology, Faculty of Chemical Engineering and Technology, Cracow University of Technology, 24 Warszawska Street, Cracow, 31-155, Poland b Polish Academy of Sciences, Hirszfeld Institute of Immunology and Experimental Therapy, Laboratory of Experimental Anticancer Therapy, Wrocław, Poland c Polish Academy of Sciences, Hirszfeld Institute of Immunology and Experimental Therapy, Laboratory of Biomedical Chemistry, Wrocław, Poland d Department of Chemical Technology and Environmental Analytics, Faculty of Chemical Engineering and Technology, Cracow University of Technology, 24 Warszawska Street, Cracow, 31-155, Poland e Institute of Physics, Faculty of Science and Technology, University of Silesia in Katowice, 75 Pułku Piechoty 1A, Chorzów, 41-500, Poland f Maj Institute of Pharmacology, Polish Academy of Sciences, Centre for the Development of Novel Pharmacotherapies for CNS Disorders (Cephares), 12 Smętna Street, Cracow, 31-343, Poland g University of Bari “Aldo Moro”, Department of Pharmacy-Pharmaceutical Sciences, 4 E. Orabona Street, Bari, I-70125, Italy h Department of Physical Chemistry, Faculty of Chemistry, Medical University of Gdańsk, 107 Gen. Hallera Alley, Gdańsk, 80-416, Poland i Laboratory of Environmental Chemoinformatics, Faculty of Chemistry, University of Gdansk, 63 Wita Stwosza Street, Gdansk, 80-308, Poland

    other:

    Article Title: A novel quinazolinone insulin receptor inhibitor and its synergy with an EGFR inhibitor in glucose-driven glioblastoma.
    Article Snippet: Both media were supplemented with 10% heat-inactivated fetal bovine serum—FBS (all reagents from Merck).

    Recombinant:

    Article Title: Compound design of a patient-derived 3D cell culture system modelling early peritoneal endometriosis
    Article Snippet: NHDFs (C-12300) isolated from the dermis of juvenile foreskin were purchased from Promocell (Heidelberg, Germany). .. NHDFs were cultured in Fibroblast Growth Medium (Promocell, C-23110) containing 1 ng/ml recombinant human basic fibroblast growth factor and 5 μg/ml recombinant human insulin, supplemented with 2 mM L-glutamine and 100 μg/ml Primocin. ..

    Isolation:

    Article Title: Sushi, von Willebrand Factor Type A, EGF and Pentraxin Domain‐Containing Protein 1: A Novel Fibroblast‐Derived Circulating Biomarker Reflecting Cardiac Fibrosis
    Article Snippet: .. Human cardiac fibroblasts (HCFs) isolated from the ventricles of adult hearts were purchased from Promocell (#C- 12375) and cultured in fibroblast growth medium (Promocell, #C- 23110) supplemented with 10% fetal bovine serum, penicillin–streptomycin, and insulin at 37 °C and 5% CO2. ..



    Similar Products

    96
    ATCC fibroblast basal medium plus fibroblast growth kitlow serum
    Fibroblast Basal Medium Plus Fibroblast Growth Kitlow Serum, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fibroblast+growth+medium/Fibroblast+Growth+Kit-Low+serum/pm42276073-414-8-16
    Average 96 stars, based on 1 article reviews
    fibroblast basal medium plus fibroblast growth kitlow serum - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    96
    PromoCell fibroblast growth medium 3
    Fibroblast Growth Medium 3, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fibroblast+growth+medium/Fibroblast+Growth+Medium+3/custom%40c-23025%4042681974
    Average 96 stars, based on 1 article reviews
    fibroblast growth medium 3 - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    96
    PromoCell fibroblast growth medium 2 media
    Fibroblast Growth Medium 2 Media, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fibroblast+growth+medium/Fibroblast+Growth+Medium+2/pm42230665-155-21-32
    Average 96 stars, based on 1 article reviews
    fibroblast growth medium 2 media - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    94
    PromoCell fibroblast growth medium
    Characterisation of primary human peritoneal mesothelial cells (HPMCs) and human peritoneal fibroblasts (HPFs) compared to the LP-9 mesothelial cell line and normal human dermal fibroblasts (NHDFs). (A) Representative phase-contrast micrographs of LP-9, HPMCs, NHDFs and HPFs. Mesothelial and <t>fibroblast</t> cells exhibit cobblestone and spindle-like morphologies, respectively. (B) We observed localised expression of the cytoskeletal markers cytokeratin (CK) and vimentin (VIM) in LP-9 cells and HPMCs, while VIM, but not CK, was expressed in NHDFs and HPFs. HPMCs ( n =6) showed a high percentage of CK + /VIM + cells [98.20±1.05% (mean±s.d.)], while HPFs ( n =3) exhibited 81.36±5.63% CK − /VIM + cells. Scale bars: 200 µm (A); 50 µm (B).
    Fibroblast Growth Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fibroblast+growth+medium/Fibroblast+Growth+Medium+Kit/pmc12919959-165-4-7
    Average 94 stars, based on 1 article reviews
    fibroblast growth medium - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    95
    PromoCell penicillin streptomycin
    Characterisation of primary human peritoneal mesothelial cells (HPMCs) and human peritoneal fibroblasts (HPFs) compared to the LP-9 mesothelial cell line and normal human dermal fibroblasts (NHDFs). (A) Representative phase-contrast micrographs of LP-9, HPMCs, NHDFs and HPFs. Mesothelial and <t>fibroblast</t> cells exhibit cobblestone and spindle-like morphologies, respectively. (B) We observed localised expression of the cytoskeletal markers cytokeratin (CK) and vimentin (VIM) in LP-9 cells and HPMCs, while VIM, but not CK, was expressed in NHDFs and HPFs. HPMCs ( n =6) showed a high percentage of CK + /VIM + cells [98.20±1.05% (mean±s.d.)], while HPFs ( n =3) exhibited 81.36±5.63% CK − /VIM + cells. Scale bars: 200 µm (A); 50 µm (B).
    Penicillin Streptomycin, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fibroblast+growth+medium/Fibroblast+Growth+Medium+3+SupplementPack/pmc13218258-219-20-14
    Average 95 stars, based on 1 article reviews
    penicillin streptomycin - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    96
    PromoCell fibroblast growth medium 2
    Characterisation of primary human peritoneal mesothelial cells (HPMCs) and human peritoneal fibroblasts (HPFs) compared to the LP-9 mesothelial cell line and normal human dermal fibroblasts (NHDFs). (A) Representative phase-contrast micrographs of LP-9, HPMCs, NHDFs and HPFs. Mesothelial and <t>fibroblast</t> cells exhibit cobblestone and spindle-like morphologies, respectively. (B) We observed localised expression of the cytoskeletal markers cytokeratin (CK) and vimentin (VIM) in LP-9 cells and HPMCs, while VIM, but not CK, was expressed in NHDFs and HPFs. HPMCs ( n =6) showed a high percentage of CK + /VIM + cells [98.20±1.05% (mean±s.d.)], while HPFs ( n =3) exhibited 81.36±5.63% CK − /VIM + cells. Scale bars: 200 µm (A); 50 µm (B).
    Fibroblast Growth Medium 2, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fibroblast+growth+medium/Fibroblast+Growth+Medium/pm42207003-103-4-10
    Average 96 stars, based on 1 article reviews
    fibroblast growth medium 2 - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    Image Search Results


    Characterisation of primary human peritoneal mesothelial cells (HPMCs) and human peritoneal fibroblasts (HPFs) compared to the LP-9 mesothelial cell line and normal human dermal fibroblasts (NHDFs). (A) Representative phase-contrast micrographs of LP-9, HPMCs, NHDFs and HPFs. Mesothelial and fibroblast cells exhibit cobblestone and spindle-like morphologies, respectively. (B) We observed localised expression of the cytoskeletal markers cytokeratin (CK) and vimentin (VIM) in LP-9 cells and HPMCs, while VIM, but not CK, was expressed in NHDFs and HPFs. HPMCs ( n =6) showed a high percentage of CK + /VIM + cells [98.20±1.05% (mean±s.d.)], while HPFs ( n =3) exhibited 81.36±5.63% CK − /VIM + cells. Scale bars: 200 µm (A); 50 µm (B).

    Journal: Disease Models & Mechanisms

    Article Title: Compound design of a patient-derived 3D cell culture system modelling early peritoneal endometriosis

    doi: 10.1242/dmm.052436

    Figure Lengend Snippet: Characterisation of primary human peritoneal mesothelial cells (HPMCs) and human peritoneal fibroblasts (HPFs) compared to the LP-9 mesothelial cell line and normal human dermal fibroblasts (NHDFs). (A) Representative phase-contrast micrographs of LP-9, HPMCs, NHDFs and HPFs. Mesothelial and fibroblast cells exhibit cobblestone and spindle-like morphologies, respectively. (B) We observed localised expression of the cytoskeletal markers cytokeratin (CK) and vimentin (VIM) in LP-9 cells and HPMCs, while VIM, but not CK, was expressed in NHDFs and HPFs. HPMCs ( n =6) showed a high percentage of CK + /VIM + cells [98.20±1.05% (mean±s.d.)], while HPFs ( n =3) exhibited 81.36±5.63% CK − /VIM + cells. Scale bars: 200 µm (A); 50 µm (B).

    Article Snippet: NHDFs were cultured in Fibroblast Growth Medium (Promocell, C-23110) containing 1 ng/ml recombinant human basic fibroblast growth factor and 5 μg/ml recombinant human insulin, supplemented with 2 mM L-glutamine and 100 μg/ml Primocin.

    Techniques: Expressing

    Establishing composite 3D hydrogel constructs using peritoneal mesothelial cells and fibroblasts. (A) Schematic illustration of model construction and culture timeline. (B) Representative axial view [also seen in C (M3)] and Haematoxylin and Eosin (H&E)-stained section of a hydrogel construct showing the formation of a mesothelial monolayer (ML) and submesothelial layer (SML) on a transwell membrane (TM). (C) Representative images of hydrogel matrices using M1 (collagen I), M2 (70:30 collagen I:Matrigel ratio), M3 (50:50 collagen I:Matrigel ratio) and M4 (collagen I+human fibronectin). Construct generated with matrix combination M3 demonstrated minimal contraction in LP-9/NHDF and HPMC/HPF trials. (D) Lactate dehydrogenase (LDH) cytotoxicity assay in M3 composite hydrogel constructs containing HPMC/HPF ( n =3 donors) over a 10-day culture period. (E) Dual immunofluorescence staining of cleaved caspase-3 (CC-3) and VIM to detect apoptotic HPMCs/HPFs in M3 constructs on day 3 and day 10 of culture ( n =3). Scale bars: 300 µm (B); 100 µm (C); 50 µm (E).

    Journal: Disease Models & Mechanisms

    Article Title: Compound design of a patient-derived 3D cell culture system modelling early peritoneal endometriosis

    doi: 10.1242/dmm.052436

    Figure Lengend Snippet: Establishing composite 3D hydrogel constructs using peritoneal mesothelial cells and fibroblasts. (A) Schematic illustration of model construction and culture timeline. (B) Representative axial view [also seen in C (M3)] and Haematoxylin and Eosin (H&E)-stained section of a hydrogel construct showing the formation of a mesothelial monolayer (ML) and submesothelial layer (SML) on a transwell membrane (TM). (C) Representative images of hydrogel matrices using M1 (collagen I), M2 (70:30 collagen I:Matrigel ratio), M3 (50:50 collagen I:Matrigel ratio) and M4 (collagen I+human fibronectin). Construct generated with matrix combination M3 demonstrated minimal contraction in LP-9/NHDF and HPMC/HPF trials. (D) Lactate dehydrogenase (LDH) cytotoxicity assay in M3 composite hydrogel constructs containing HPMC/HPF ( n =3 donors) over a 10-day culture period. (E) Dual immunofluorescence staining of cleaved caspase-3 (CC-3) and VIM to detect apoptotic HPMCs/HPFs in M3 constructs on day 3 and day 10 of culture ( n =3). Scale bars: 300 µm (B); 100 µm (C); 50 µm (E).

    Article Snippet: NHDFs were cultured in Fibroblast Growth Medium (Promocell, C-23110) containing 1 ng/ml recombinant human basic fibroblast growth factor and 5 μg/ml recombinant human insulin, supplemented with 2 mM L-glutamine and 100 μg/ml Primocin.

    Techniques: Construct, Staining, Membrane, Generated, LDH Cytotoxicity Assay, Immunofluorescence

    Histological and functional analysis of the human parietal peritoneum and peritoneal layer models. (A) Histological staining of transverse sections through parietal peritoneum and composite 3D hydrogel constructs composed of LP-9/NHDFs and HPMCs/HPFs. Immunofluorescence using antibodies against the mesothelial markers podoplanin (PDPN) and mesothelin (MSLN), and submesothelial markers fibroblast specific protein 1 (FSP1) and tumor endothelial marker 1 (TEM1). (B) Colocalisation of MSLN and collagen IV (COLIV) suggesting spontaneous basal lamina formation. (C) Human tissue plasminogen activator (tPA) enzyme-linked immunosorbent assay (ELISA) to determine the functionality of the mesothelial cells in models assembled with HPMCs from three different donors over a 10-day culture period. Scale bars: 50 µm; 15 µm (insets in B).

    Journal: Disease Models & Mechanisms

    Article Title: Compound design of a patient-derived 3D cell culture system modelling early peritoneal endometriosis

    doi: 10.1242/dmm.052436

    Figure Lengend Snippet: Histological and functional analysis of the human parietal peritoneum and peritoneal layer models. (A) Histological staining of transverse sections through parietal peritoneum and composite 3D hydrogel constructs composed of LP-9/NHDFs and HPMCs/HPFs. Immunofluorescence using antibodies against the mesothelial markers podoplanin (PDPN) and mesothelin (MSLN), and submesothelial markers fibroblast specific protein 1 (FSP1) and tumor endothelial marker 1 (TEM1). (B) Colocalisation of MSLN and collagen IV (COLIV) suggesting spontaneous basal lamina formation. (C) Human tissue plasminogen activator (tPA) enzyme-linked immunosorbent assay (ELISA) to determine the functionality of the mesothelial cells in models assembled with HPMCs from three different donors over a 10-day culture period. Scale bars: 50 µm; 15 µm (insets in B).

    Article Snippet: NHDFs were cultured in Fibroblast Growth Medium (Promocell, C-23110) containing 1 ng/ml recombinant human basic fibroblast growth factor and 5 μg/ml recombinant human insulin, supplemented with 2 mM L-glutamine and 100 μg/ml Primocin.

    Techniques: Functional Assay, Staining, Construct, Immunofluorescence, Marker, Enzyme-linked Immunosorbent Assay