recombinant human fgf-2 (a concentration of at 200 ng/ml)  (R&D Systems)

Journal: Cell Proliferation

Article Title: Possibility of selection of chondrogenic progenitor cells by telomere length in FGF‐2‐expanded mesenchymal stromal cells

doi: 10.1111/j.1365-2184.2006.00397.x

Figure Lengend Snippet: Cell proliferation and morphological aspects. (a) Proliferation curve of FGF(+)MSC or FGF(–)MSC from MSC19 and 45 (donor age, 19 and 45 years old, respectively). MSCs were isolated from bone marrow aspirates of normal adult donors. After confirming cell adhesion to the plate, MSCs were cultured with 15% FBS‐DMEM with or without FGF‐2 (final conc.; 1 ng/ml). Cumulative population doublings level (PDL) is regarded as zero for culture starting immediately after the primary culture of cells, and calculated to increase according to the equation: log2{(the number of collected cells)/(the number of seeded cells)}. (b) Representative morphological aspects. FGF+; cells cultured with media including FGF‐2, FGF−; without FGF‐2. The scale indicates 5 µm.

Article Snippet: After confirming cell adhesion, MSCs were cultured with or without FGF‐2 (final concentration; 1 ng/ml, R & D Systems Inc. Minneapolis, USA).

Techniques: Isolation, Cell Culture

Journal: Cell Proliferation

Article Title: Possibility of selection of chondrogenic progenitor cells by telomere length in FGF‐2‐expanded mesenchymal stromal cells

doi: 10.1111/j.1365-2184.2006.00397.x

Figure Lengend Snippet: Effect of FGF‐2 on telomere length of MSC. Telomere length was measured by Southern blot analysis. MSC14 and 19: donor age, 14 and 19 years old. h‐Chon79: human chondrocytes, 79 years old. Chon. F+ or Chon. F−; chondrogenesis of FGF(+)MSC or FGF(–)MSC.

Article Snippet: After confirming cell adhesion, MSCs were cultured with or without FGF‐2 (final concentration; 1 ng/ml, R & D Systems Inc. Minneapolis, USA).

Techniques: Southern Blot