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f2rl1 par2  (MedChemExpress)


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    MedChemExpress f2rl1 par2
    F2rl1 Par2, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/f2rl1+par2/PAR2+Antibody/pmc12459351-285-31-32
    Average 93 stars, based on 1 article reviews
    f2rl1 par2 - by Bioz Stars, 2026-10
    93/100 stars

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    Article Title: Neutrophil-derived serine proteases induce FOXA2-mediated autophagy dysfunction and exacerbate colitis-associated carcinogenesis via protease activated receptor 2
    Article Snippet: AOM (Sigma-Aldrich, A5486), dextran sulfate sodium salt (MP Biomedicals, MFCD00081551), LysoSensorTM Green DND-189 (Thermo Scientific, L7535), LysoTracker Deep Red (Thermo Scientific, L12492 ), cathepsin B Assay (Abcam, ab270772), GB88 (MedChemExpress, HY-120261), F2RL1/PAR2-IN-1 (MedChemExpress, HY-138558), F2RL1/PAR2-AP (Shanghai Apeptide, N/A), ENMD-1068 (Abcam, ab141699), mitoSox Green (Thermo Scientific, M36005 ), Lipofectamine 2000 (Thermo Scientific 11668019), DAPI (Sigma-Aldrich, F6057), polyethylenimine (Polysciences 23966–2), TB Green® Premix Ex TaqTM (TAKARA, RR820A), actinomycin D (MedChemExpress, HY-17559), bafilomycin A 1 (MedChemExpress, HY-100558), L-Glutamine (Thermo Scientific 25030081), Cell Dissociation Buffer Enzyme-Free PBS-based (Gibco 13151–014).

    Article Title: Neutrophil-derived serine proteases induce FOXA2-mediated autophagy dysfunction and exacerbate colitis-associated carcinogenesis via protease activated receptor 2.
    Article Snippet: AOM (Sigma-Aldrich, A5486), dextran sulfate sodium salt (MP Biomedicals, MFCD00081551), LysoSensorTM Green DND-189 (Thermo Scientific, L7535), LysoTracker Deep Red (Thermo Scientific, L12492), cathepsin B Assay (Abcam, ab270772), GB88 (MedChemExpress, HY-120261), F2RL1/PAR2-IN-1 (MedChemExpress, HY-138558), F2RL1/PAR2-AP (Shanghai Apeptide, N/A), ENMD-1068 (Abcam, ab141699), mitoSox Green (Thermo Scientific, M36005), AC CE PT ED M AN US CR IP T Lipofectamine 2000 (Thermo Scientific, 11668019), DAPI (Sigma-Aldrich, F6057), polyethylenimine (Polysciences, 23966-2), TB Green® Premix Ex TaqTM (TAKARA, RR820A), actinomycin D (MedChemExpress, HY-17559), bafilomycin A1 (MedChemExpress, HY-100558), L-Glutamine (Thermo Scientific, 25030081), Cell Dissociation Buffer Enzyme-Free PBS-based (Gibco, 13151-014 ).



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    ( A ) R in Alp1-challenged F2rl1 −/ − mice that lack <t>PAR2</t> expression (two to seven mice per group). ( B and C ) Total cell counts (B) and cell composition (C) in BALF. (B) * p = 0.01, unpaired t test. ( D ) Lung inflammation and airway mucin evaluated by H& E (left) and PAS (right) staining. Images are representative of three to five mice per group. Original magnification ×10. ( E ) PAS + area within the airway epithelium. ( F ) IL-4, IL-5, and IL-13 levels in BALF. All symbols in bar graphs represent results from an individual mouse; error bars are mean ± SEM.; all results represent at least two independent experiments.
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    PAR2 expression level in lung cancer cells transfected either with pcDNA3-PAR2 or PAR2 shRNA. (A) PAR2 expression level after A549 cells were transfected with pcDNA3-PAR2; (B) PAR2 expression level after H1299 cells were transfected with pcDNA3-PAR2; (C) PAR2 expression level after A549 cells were transfected with PAR2 shRNA; (D) PAR2 expression level after H1299 cells were transfected with PAR2 shRNA.

    Journal: Future Science OA

    Article Title: PAR2 regulates proliferation, migration of lung cancer and chemotherapy sensitivity by involving PTEN pathway

    doi: 10.1080/20565623.2025.2535221

    Figure Lengend Snippet: PAR2 expression level in lung cancer cells transfected either with pcDNA3-PAR2 or PAR2 shRNA. (A) PAR2 expression level after A549 cells were transfected with pcDNA3-PAR2; (B) PAR2 expression level after H1299 cells were transfected with pcDNA3-PAR2; (C) PAR2 expression level after A549 cells were transfected with PAR2 shRNA; (D) PAR2 expression level after H1299 cells were transfected with PAR2 shRNA.

    Article Snippet: The primers were synthesized by Origene (USA) and the sequences were as follow: PAR2 forward: 5′-CTCCTCTCTGTCATCTGGTTCC-3′ and reverse 5′-TGCACACTGAGGCAGGTCATGA-3′.

    Techniques: Expressing, Transfection, shRNA

    Overexpression PAR2 promoted growth of lung cancer cells with or without paclitaxel. (A–D) The growth of A549 cells with or without paclitaxel after transfection with pcDNA3-PAR2; (E–H) The growth of H1299 cells with or without paclitaxel after transfection with pcDNA3-PAR2.

    Journal: Future Science OA

    Article Title: PAR2 regulates proliferation, migration of lung cancer and chemotherapy sensitivity by involving PTEN pathway

    doi: 10.1080/20565623.2025.2535221

    Figure Lengend Snippet: Overexpression PAR2 promoted growth of lung cancer cells with or without paclitaxel. (A–D) The growth of A549 cells with or without paclitaxel after transfection with pcDNA3-PAR2; (E–H) The growth of H1299 cells with or without paclitaxel after transfection with pcDNA3-PAR2.

    Article Snippet: The primers were synthesized by Origene (USA) and the sequences were as follow: PAR2 forward: 5′-CTCCTCTCTGTCATCTGGTTCC-3′ and reverse 5′-TGCACACTGAGGCAGGTCATGA-3′.

    Techniques: Over Expression, Transfection

    Knockdown PAR2 decreased growth of lung cancer cells with or without paclitaxel. (A–D) The growth of A549 cells with or without paclitaxel after transfection with PAR2 shRNA; (E–H) The growth of H1299 cells with or without paclitaxel after transfection with PAR2 shRNA.

    Journal: Future Science OA

    Article Title: PAR2 regulates proliferation, migration of lung cancer and chemotherapy sensitivity by involving PTEN pathway

    doi: 10.1080/20565623.2025.2535221

    Figure Lengend Snippet: Knockdown PAR2 decreased growth of lung cancer cells with or without paclitaxel. (A–D) The growth of A549 cells with or without paclitaxel after transfection with PAR2 shRNA; (E–H) The growth of H1299 cells with or without paclitaxel after transfection with PAR2 shRNA.

    Article Snippet: The primers were synthesized by Origene (USA) and the sequences were as follow: PAR2 forward: 5′-CTCCTCTCTGTCATCTGGTTCC-3′ and reverse 5′-TGCACACTGAGGCAGGTCATGA-3′.

    Techniques: Knockdown, Transfection, shRNA

    PAR2 inhibited paclitaxel-associated apoptosis in lung cancer cells. (A) Caspase 3/7 activity in A549 cell transfected with pcDNA3-PAR2; (B) Caspase 3/7 activity in H1299 cell transfected with pcDNA3-PAR2; (C–F) Bcl-2 and BAX expression in A549 and H1299 cells.

    Journal: Future Science OA

    Article Title: PAR2 regulates proliferation, migration of lung cancer and chemotherapy sensitivity by involving PTEN pathway

    doi: 10.1080/20565623.2025.2535221

    Figure Lengend Snippet: PAR2 inhibited paclitaxel-associated apoptosis in lung cancer cells. (A) Caspase 3/7 activity in A549 cell transfected with pcDNA3-PAR2; (B) Caspase 3/7 activity in H1299 cell transfected with pcDNA3-PAR2; (C–F) Bcl-2 and BAX expression in A549 and H1299 cells.

    Article Snippet: The primers were synthesized by Origene (USA) and the sequences were as follow: PAR2 forward: 5′-CTCCTCTCTGTCATCTGGTTCC-3′ and reverse 5′-TGCACACTGAGGCAGGTCATGA-3′.

    Techniques: Activity Assay, Transfection, Expressing

    PAR2 played essential roles in invasion and migration of lung cancer cells. (A, B) up-regulation of PAR2 increased migration and invasion of A549 cells. (C, D) down-regulation of PAR2 decreaased migration and invasion of A549 cells. (E, F) up-regulation of PAR2 increased migration and invasion of H1299 cells. (G, H) down-regulation of PAR2 decreased migration and invasion of H1299 cells.

    Journal: Future Science OA

    Article Title: PAR2 regulates proliferation, migration of lung cancer and chemotherapy sensitivity by involving PTEN pathway

    doi: 10.1080/20565623.2025.2535221

    Figure Lengend Snippet: PAR2 played essential roles in invasion and migration of lung cancer cells. (A, B) up-regulation of PAR2 increased migration and invasion of A549 cells. (C, D) down-regulation of PAR2 decreaased migration and invasion of A549 cells. (E, F) up-regulation of PAR2 increased migration and invasion of H1299 cells. (G, H) down-regulation of PAR2 decreased migration and invasion of H1299 cells.

    Article Snippet: The primers were synthesized by Origene (USA) and the sequences were as follow: PAR2 forward: 5′-CTCCTCTCTGTCATCTGGTTCC-3′ and reverse 5′-TGCACACTGAGGCAGGTCATGA-3′.

    Techniques: Migration

    PAR2 altered PTEN/AKT protein expression in lung cancer cells. (A–C) Impact of up-regulation of PAR2 on expression of p-AKT, AKT and PTEN in A549 and H1299 cells. (D–F) Impact of down-regulation of PAR2 on expression of p-AKT, AKT and PTEN in A549 and H1299 cells.

    Journal: Future Science OA

    Article Title: PAR2 regulates proliferation, migration of lung cancer and chemotherapy sensitivity by involving PTEN pathway

    doi: 10.1080/20565623.2025.2535221

    Figure Lengend Snippet: PAR2 altered PTEN/AKT protein expression in lung cancer cells. (A–C) Impact of up-regulation of PAR2 on expression of p-AKT, AKT and PTEN in A549 and H1299 cells. (D–F) Impact of down-regulation of PAR2 on expression of p-AKT, AKT and PTEN in A549 and H1299 cells.

    Article Snippet: The primers were synthesized by Origene (USA) and the sequences were as follow: PAR2 forward: 5′-CTCCTCTCTGTCATCTGGTTCC-3′ and reverse 5′-TGCACACTGAGGCAGGTCATGA-3′.

    Techniques: Expressing

    PAR2 expression in human lung cancer tissue. (A) PAR2 levels in different stage of lung cancer tissue and normal lung tissue. (B) Immunohistochemical studies for Ki-67 and PTEN on different levels of PAR2 in lung tissue.

    Journal: Future Science OA

    Article Title: PAR2 regulates proliferation, migration of lung cancer and chemotherapy sensitivity by involving PTEN pathway

    doi: 10.1080/20565623.2025.2535221

    Figure Lengend Snippet: PAR2 expression in human lung cancer tissue. (A) PAR2 levels in different stage of lung cancer tissue and normal lung tissue. (B) Immunohistochemical studies for Ki-67 and PTEN on different levels of PAR2 in lung tissue.

    Article Snippet: The primers were synthesized by Origene (USA) and the sequences were as follow: PAR2 forward: 5′-CTCCTCTCTGTCATCTGGTTCC-3′ and reverse 5′-TGCACACTGAGGCAGGTCATGA-3′.

    Techniques: Expressing, Immunohistochemical staining

    ( A ) R in Alp1-challenged F2rl1 −/ − mice that lack PAR2 expression (two to seven mice per group). ( B and C ) Total cell counts (B) and cell composition (C) in BALF. (B) * p = 0.01, unpaired t test. ( D ) Lung inflammation and airway mucin evaluated by H& E (left) and PAS (right) staining. Images are representative of three to five mice per group. Original magnification ×10. ( E ) PAS + area within the airway epithelium. ( F ) IL-4, IL-5, and IL-13 levels in BALF. All symbols in bar graphs represent results from an individual mouse; error bars are mean ± SEM.; all results represent at least two independent experiments.

    Journal: ImmunoHorizons

    Article Title: Aspergillus fumigatus –Secreted Alkaline Protease 1 Mediates Airways Hyperresponsiveness in Severe Asthma

    doi: 10.4049/immunohorizons.1900046

    Figure Lengend Snippet: ( A ) R in Alp1-challenged F2rl1 −/ − mice that lack PAR2 expression (two to seven mice per group). ( B and C ) Total cell counts (B) and cell composition (C) in BALF. (B) * p = 0.01, unpaired t test. ( D ) Lung inflammation and airway mucin evaluated by H& E (left) and PAS (right) staining. Images are representative of three to five mice per group. Original magnification ×10. ( E ) PAS + area within the airway epithelium. ( F ) IL-4, IL-5, and IL-13 levels in BALF. All symbols in bar graphs represent results from an individual mouse; error bars are mean ± SEM.; all results represent at least two independent experiments.

    Article Snippet: BALB/c, C57BL/6, Δdbl-gata , and protease-activated receptor 2 (PAR2)–deficient ( F2rl1 −/− ) mice were obtained from The Jackson Laboratory.

    Techniques: Expressing, Staining