Sequencing:Article Title: AAT Rhg1 is a tonoplast protein that alters amino acid, metabolic and defense responses and nematode resistance
Article Snippet: .. A synthetic artificial RNAi-escaping Rhg1-GmAAT coding sequence driven by a GmUbi promoter was created by designing synonymous codon substitutions with preference for commonly used soybean codons, synthesized at Twist Bioscience (South San Francisco, CA, USA) and deployed in binary vector pAGM4673, and also modified by site-directed mutagenesis to generate analogous SynAATRhg1Y268L or SynAATRhg1D122A constructs (Supplemental Information). .. A synthetic artificial RNAi-escaping Rhg1-GmAAT coding sequence driven by a GmUbi promoter was created by designing synonymous codon substitutions with preference for commonly used soybean codons, synthesized at Twist Bioscience (South San Francisco, CA, USA) and deployed in binary vector pAGM4673, and also modified by site-directed mutagenesis to generate analogous SynAATRhg1Y268L or SynAATRhg1D122A constructs (Supplemental Information).
Article Title: Mutations outside the MR1 antigen binding groove differentially inhibit presentation of exogenous antigens
Article Snippet: .. To overexpress B2M in the D6 clonal cell line, the B2M coding sequence (NCBI entry NM_004048.4 ) with a downstream internal ribosomal entry site GFP ( ) was ordered from Twist Biosciences and cloned into the pCI AscI mammalian expression vector previously described ( ). .. The mass spectrometry proteomics data have been deposited to the ProteomeXchange ( ) Consortium via the PRIDE ( ) partner repository with the dataset identifier PXD062277.
Synthesized:Article Title: AAT Rhg1 is a tonoplast protein that alters amino acid, metabolic and defense responses and nematode resistance
Article Snippet: .. A synthetic artificial RNAi-escaping Rhg1-GmAAT coding sequence driven by a GmUbi promoter was created by designing synonymous codon substitutions with preference for commonly used soybean codons, synthesized at Twist Bioscience (South San Francisco, CA, USA) and deployed in binary vector pAGM4673, and also modified by site-directed mutagenesis to generate analogous SynAATRhg1Y268L or SynAATRhg1D122A constructs (Supplemental Information). .. A synthetic artificial RNAi-escaping Rhg1-GmAAT coding sequence driven by a GmUbi promoter was created by designing synonymous codon substitutions with preference for commonly used soybean codons, synthesized at Twist Bioscience (South San Francisco, CA, USA) and deployed in binary vector pAGM4673, and also modified by site-directed mutagenesis to generate analogous SynAATRhg1Y268L or SynAATRhg1D122A constructs (Supplemental Information).
Article Title: Physiologic variation in sperm miRNAs tune embryonic gene regulatory programs and developmental outcomes
Article Snippet: .. The RNA Adenosine Base Editor (rABE) developed by the Floor laboratory was codon-optimized for Mus musculus and synthesized to generate fusion constructs (Twist Bioscience). rABE was fused to either AGO2 or mRUBY3 via a flexible peptide linker (DSGGSSGGSSGSETPGTSESATPESSGGSSGGS) positioned at the C-terminus of rABE. .. The RNA Adenosine Base Editor (rABE) developed by the Floor laboratory was codon-optimized for Mus musculus and synthesized to generate fusion constructs (Twist Bioscience).
Plasmid Preparation:Article Title: AAT Rhg1 is a tonoplast protein that alters amino acid, metabolic and defense responses and nematode resistance
Article Snippet: .. A synthetic artificial RNAi-escaping Rhg1-GmAAT coding sequence driven by a GmUbi promoter was created by designing synonymous codon substitutions with preference for commonly used soybean codons, synthesized at Twist Bioscience (South San Francisco, CA, USA) and deployed in binary vector pAGM4673, and also modified by site-directed mutagenesis to generate analogous SynAATRhg1Y268L or SynAATRhg1D122A constructs (Supplemental Information). .. A synthetic artificial RNAi-escaping Rhg1-GmAAT coding sequence driven by a GmUbi promoter was created by designing synonymous codon substitutions with preference for commonly used soybean codons, synthesized at Twist Bioscience (South San Francisco, CA, USA) and deployed in binary vector pAGM4673, and also modified by site-directed mutagenesis to generate analogous SynAATRhg1Y268L or SynAATRhg1D122A constructs (Supplemental Information).
Article Title: De novo masking domains that gate TNF-family ligand assembly and activity
Article Snippet: .. All constructs were cloned into the pTwist cDNA 3.4 mammalian expression vector (Twist Bioscience) and transiently expressed in Expi293F cells (Thermo Fisher Scientific) using a PEI-based transfection protocol adapted from a published method . .. Cells were transfected at a density of 2.5 × 10 cells/mL with PEI MAX (Kyfora Bio) and plasmid DNA.
Article Title: Mutations outside the MR1 antigen binding groove differentially inhibit presentation of exogenous antigens
Article Snippet: .. To overexpress B2M in the D6 clonal cell line, the B2M coding sequence (NCBI entry NM_004048.4 ) with a downstream internal ribosomal entry site GFP ( ) was ordered from Twist Biosciences and cloned into the pCI AscI mammalian expression vector previously described ( ). .. The mass spectrometry proteomics data have been deposited to the ProteomeXchange ( ) Consortium via the PRIDE ( ) partner repository with the dataset identifier PXD062277.
Article Title: CD164 is an endolysomal host factor for entry of Clade A New World Arenaviruses
Article Snippet: .. Clade A NWA GP1 constructs were cloned into a pTwist-CMV-ßGlobin mammalian expression vector (Twist Bioscience Inc.) under a CMV promoter with a N-terminal CD5 signal peptide with a C-terminal twin-strep tag fused to the GP1 with a Gly-Ser linker. .. Cells were then maintained at respective pH on ice and subjected to flow cytometry analysis (Beckman Coulter Cytoflex S), collecting 50,000 singlets/condition and analyzed for cells, singlets, and AF647 mean fluorescent intensity (MFI) using FlowJo (version 10) software.
Modification:Article Title: AAT Rhg1 is a tonoplast protein that alters amino acid, metabolic and defense responses and nematode resistance
Article Snippet: .. A synthetic artificial RNAi-escaping Rhg1-GmAAT coding sequence driven by a GmUbi promoter was created by designing synonymous codon substitutions with preference for commonly used soybean codons, synthesized at Twist Bioscience (South San Francisco, CA, USA) and deployed in binary vector pAGM4673, and also modified by site-directed mutagenesis to generate analogous SynAATRhg1Y268L or SynAATRhg1D122A constructs (Supplemental Information). .. A synthetic artificial RNAi-escaping Rhg1-GmAAT coding sequence driven by a GmUbi promoter was created by designing synonymous codon substitutions with preference for commonly used soybean codons, synthesized at Twist Bioscience (South San Francisco, CA, USA) and deployed in binary vector pAGM4673, and also modified by site-directed mutagenesis to generate analogous SynAATRhg1Y268L or SynAATRhg1D122A constructs (Supplemental Information).
Mutagenesis:Article Title: AAT Rhg1 is a tonoplast protein that alters amino acid, metabolic and defense responses and nematode resistance
Article Snippet: .. A synthetic artificial RNAi-escaping Rhg1-GmAAT coding sequence driven by a GmUbi promoter was created by designing synonymous codon substitutions with preference for commonly used soybean codons, synthesized at Twist Bioscience (South San Francisco, CA, USA) and deployed in binary vector pAGM4673, and also modified by site-directed mutagenesis to generate analogous SynAATRhg1Y268L or SynAATRhg1D122A constructs (Supplemental Information). .. A synthetic artificial RNAi-escaping Rhg1-GmAAT coding sequence driven by a GmUbi promoter was created by designing synonymous codon substitutions with preference for commonly used soybean codons, synthesized at Twist Bioscience (South San Francisco, CA, USA) and deployed in binary vector pAGM4673, and also modified by site-directed mutagenesis to generate analogous SynAATRhg1Y268L or SynAATRhg1D122A constructs (Supplemental Information).
Construct:Article Title: AAT Rhg1 is a tonoplast protein that alters amino acid, metabolic and defense responses and nematode resistance
Article Snippet: .. A synthetic artificial RNAi-escaping Rhg1-GmAAT coding sequence driven by a GmUbi promoter was created by designing synonymous codon substitutions with preference for commonly used soybean codons, synthesized at Twist Bioscience (South San Francisco, CA, USA) and deployed in binary vector pAGM4673, and also modified by site-directed mutagenesis to generate analogous SynAATRhg1Y268L or SynAATRhg1D122A constructs (Supplemental Information). .. A synthetic artificial RNAi-escaping Rhg1-GmAAT coding sequence driven by a GmUbi promoter was created by designing synonymous codon substitutions with preference for commonly used soybean codons, synthesized at Twist Bioscience (South San Francisco, CA, USA) and deployed in binary vector pAGM4673, and also modified by site-directed mutagenesis to generate analogous SynAATRhg1Y268L or SynAATRhg1D122A constructs (Supplemental Information).
Article Title: De novo masking domains that gate TNF-family ligand assembly and activity
Article Snippet: .. All constructs were cloned into the pTwist cDNA 3.4 mammalian expression vector (Twist Bioscience) and transiently expressed in Expi293F cells (Thermo Fisher Scientific) using a PEI-based transfection protocol adapted from a published method . .. Cells were transfected at a density of 2.5 × 10 cells/mL with PEI MAX (Kyfora Bio) and plasmid DNA.
Article Title: Physiologic variation in sperm miRNAs tune embryonic gene regulatory programs and developmental outcomes
Article Snippet: .. The RNA Adenosine Base Editor (rABE) developed by the Floor laboratory was codon-optimized for Mus musculus and synthesized to generate fusion constructs (Twist Bioscience). rABE was fused to either AGO2 or mRUBY3 via a flexible peptide linker (DSGGSSGGSSGSETPGTSESATPESSGGSSGGS) positioned at the C-terminus of rABE. .. The RNA Adenosine Base Editor (rABE) developed by the Floor laboratory was codon-optimized for Mus musculus and synthesized to generate fusion constructs (Twist Bioscience).
Article Title: Stereoselective methyl-swapping demonstrates target specificity of cognitive enhancer
Article Snippet: .. For the expression of human trimeric eIF2, eukaryotic expression plasmids pALT002 (full-length eIF2α), pALT003 (FLAG-TEV-eIF2β), and pALT004 (full-length eIF2γ) were constructed and sequenced by Twist Bioscience. .. The lentiviral ATF4-mVenus reporter plasmid (pMB033) was constructed by Vectorbuilder.
Article Title: CD164 is an endolysomal host factor for entry of Clade A New World Arenaviruses
Article Snippet: .. Clade A NWA GP1 constructs were cloned into a pTwist-CMV-ßGlobin mammalian expression vector (Twist Bioscience Inc.) under a CMV promoter with a N-terminal CD5 signal peptide with a C-terminal twin-strep tag fused to the GP1 with a Gly-Ser linker. .. Cells were then maintained at respective pH on ice and subjected to flow cytometry analysis (Beckman Coulter Cytoflex S), collecting 50,000 singlets/condition and analyzed for cells, singlets, and AF647 mean fluorescent intensity (MFI) using FlowJo (version 10) software.
Clone Assay:Article Title: De novo masking domains that gate TNF-family ligand assembly and activity
Article Snippet: .. All constructs were cloned into the pTwist cDNA 3.4 mammalian expression vector (Twist Bioscience) and transiently expressed in Expi293F cells (Thermo Fisher Scientific) using a PEI-based transfection protocol adapted from a published method . .. Cells were transfected at a density of 2.5 × 10 cells/mL with PEI MAX (Kyfora Bio) and plasmid DNA.
Article Title: Mutations outside the MR1 antigen binding groove differentially inhibit presentation of exogenous antigens
Article Snippet: .. To overexpress B2M in the D6 clonal cell line, the B2M coding sequence (NCBI entry NM_004048.4 ) with a downstream internal ribosomal entry site GFP ( ) was ordered from Twist Biosciences and cloned into the pCI AscI mammalian expression vector previously described ( ). .. The mass spectrometry proteomics data have been deposited to the ProteomeXchange ( ) Consortium via the PRIDE ( ) partner repository with the dataset identifier PXD062277.
Article Title: CD164 is an endolysomal host factor for entry of Clade A New World Arenaviruses
Article Snippet: .. Clade A NWA GP1 constructs were cloned into a pTwist-CMV-ßGlobin mammalian expression vector (Twist Bioscience Inc.) under a CMV promoter with a N-terminal CD5 signal peptide with a C-terminal twin-strep tag fused to the GP1 with a Gly-Ser linker. .. Cells were then maintained at respective pH on ice and subjected to flow cytometry analysis (Beckman Coulter Cytoflex S), collecting 50,000 singlets/condition and analyzed for cells, singlets, and AF647 mean fluorescent intensity (MFI) using FlowJo (version 10) software.
Expressing:Article Title: De novo masking domains that gate TNF-family ligand assembly and activity
Article Snippet: .. All constructs were cloned into the pTwist cDNA 3.4 mammalian expression vector (Twist Bioscience) and transiently expressed in Expi293F cells (Thermo Fisher Scientific) using a PEI-based transfection protocol adapted from a published method . .. Cells were transfected at a density of 2.5 × 10 cells/mL with PEI MAX (Kyfora Bio) and plasmid DNA.
Article Title: Stereoselective methyl-swapping demonstrates target specificity of cognitive enhancer
Article Snippet: .. For the expression of human trimeric eIF2, eukaryotic expression plasmids pALT002 (full-length eIF2α), pALT003 (FLAG-TEV-eIF2β), and pALT004 (full-length eIF2γ) were constructed and sequenced by Twist Bioscience. .. The lentiviral ATF4-mVenus reporter plasmid (pMB033) was constructed by Vectorbuilder.
Article Title: Mutations outside the MR1 antigen binding groove differentially inhibit presentation of exogenous antigens
Article Snippet: .. To overexpress B2M in the D6 clonal cell line, the B2M coding sequence (NCBI entry NM_004048.4 ) with a downstream internal ribosomal entry site GFP ( ) was ordered from Twist Biosciences and cloned into the pCI AscI mammalian expression vector previously described ( ). .. The mass spectrometry proteomics data have been deposited to the ProteomeXchange ( ) Consortium via the PRIDE ( ) partner repository with the dataset identifier PXD062277.
Article Title: CD164 is an endolysomal host factor for entry of Clade A New World Arenaviruses
Article Snippet: .. Clade A NWA GP1 constructs were cloned into a pTwist-CMV-ßGlobin mammalian expression vector (Twist Bioscience Inc.) under a CMV promoter with a N-terminal CD5 signal peptide with a C-terminal twin-strep tag fused to the GP1 with a Gly-Ser linker. .. Cells were then maintained at respective pH on ice and subjected to flow cytometry analysis (Beckman Coulter Cytoflex S), collecting 50,000 singlets/condition and analyzed for cells, singlets, and AF647 mean fluorescent intensity (MFI) using FlowJo (version 10) software.
Transfection:Article Title: De novo masking domains that gate TNF-family ligand assembly and activity
Article Snippet: .. All constructs were cloned into the pTwist cDNA 3.4 mammalian expression vector (Twist Bioscience) and transiently expressed in Expi293F cells (Thermo Fisher Scientific) using a PEI-based transfection protocol adapted from a published method . .. Cells were transfected at a density of 2.5 × 10 cells/mL with PEI MAX (Kyfora Bio) and plasmid DNA.
other:Article Title: Programmable Nucleic Acid Sensing in Human Cells Using Circularizable ssDNA.
Article Snippet: AR TI CL E IN P RE SS To construct Nluc reporter plasmids for evaluating SONAR activity with a Cas12 payload, synthetic gBlocks (IDT) encoding an mCherry sequence, a Cas12 target site, and an out-of-frame Nluc coding sequence were designed and cloned downstream of the CMV promoter into the plasmid backbone (Addgene #182540), which had been digested with NheI and XbaI, using the NEB HiFi DNA Assembly Master Mix.
|
Twist Bioscience is a verified supplier 
Twist Bioscience manufactures this product 
![a IgG (negative control), H3K27ac (positive control), TBL1X, TBL1XR1, and PAX6 ChIP-qPCR in EndoC-βH1 cells for the human INS locus. The enrichment is calculated over the negative locus and the IgG control. n = 3 individual experiments. b Endogenous TBL1X, TBL1XR1, HDAC3, and SPT16 were immunoprecipitated in EndoC-βH1 lysates with rabbit IgG as negative control. Whole cell lysates (Input) and immunoprecipitated proteins were immunoblotted using the indicated antibodies. Representative blot is shown for 4 independent experiments. c Human INS ( hINS ) promoter activity in INS1E cells upon TBL/R1 knockdown. Luciferase activity normalized to renilla luciferase. n = 4 wells/condition. d Insulin secretion relative to insulin content in EndoC-βH1 cells upon TBL/R1 knockdown. After glucose starvation, insulin secretion was stimulated using 2.8 mM glucose (2.8 G) and 2.8 mM glucose supplemented with KCl (KCl). n = 4 wells/condition. e Scatter plot depicting the relationship between HbA1c [%] and relative TBL1X mRNA levels determined by qPCR, adjusted for age, body mass index (BMI), and disease state. Each dot represents an individual data point. n = 31. f , g Manhattan plot showing an association between elevated HbA1c levels and single nucleotide polymorphisms in the genetic region of TBL1X ( f ) and TBL1XR1 ( g ). Data are represented as mean ± SEM. The following statistical tests were applied: two-sided student’s t test ( a , c , d ), and linear regression ( e ). Source data are provided as a Source Data file.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_2947/pmc13102947/pmc13102947__41467_2026_72077_Fig6_HTML.jpg)