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standard pulsed edu  (Thermo Fisher)


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    Thermo Fisher standard pulsed edu
    Standard Pulsed Edu, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ethynyl/EdU+(5-ethynyl-2%E2%80%99-deoxyuridine)/10__1038_slash_s41467___026___78035___5-382-0-14
    Average 97 stars, based on 1 article reviews
    standard pulsed edu - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    Incubation:

    Article Title: Replication stress induced exposure to methotrexate in root meristem cells of Vicia faba.
    Article Snippet: .. Root meristems from all experimental series were incubated with 10 μM 5-ethynyl-2′-deoxyuridine (EdU; Thermo Fisher Scientific, Warsaw, Poland) under darkened conditions. .. After 30 min, the dissected meristems were fixed in 4% phosphate buffered saline (PBS)–buffered paraformaldehyde (4 °C; 20 min; pH 7.4; Merck Life Science, Poznan, Poland) and then macerated with 2.5% citrate-buffered pectinase (pH 5.0; 30 min; Merck Life Science, Poznan, Poland).

    Article Title: Limitations of the p16-3MR mouse model for detecting and eliminating senescent cells.
    Article Snippet: .. For proliferation assays, MEFs were incubated with 2 μM 5-ethynyl-2′-deoxyuridine (EdU) for 3 days, fixed with 4% paraformaldehyde (PFA), and stained using the Click-iT EdU Cell Proliferation Kit for Imaging (C10640; Invitrogen). .. For cell viability assays, MEFs were treated with 10 μg/mL GCV or vehicle (Dimethyl sulfoxide (DMSO; 13445-74, Nacalai tesque)) for 6 days, © The Author(s) EMBO reports 11 with medium replaced every 2 days.

    Injection:

    Article Title: Wnt Regulates Cellular Differentiation and Retinoid Metabolism Genes in the Mouse Stomach.
    Article Snippet: Gastric organoids generated from wild-type C57BL/6 mice (Jackson Labs 118 #000664) were used for bulk RNA sequencing experiments. .. For analysis of proliferation, mice 119 were injected with 5-ethynyl-2’-deoxyuridine (EdU, 25 mg/kg, Invitrogen) 2 hours prior to tissue 120 collection. ..

    Article Title: Persistent and transient senescent cells contribute to brain-barrier development.
    Article Snippet: .. 5-ethynyl-2’-deoxyuridine (EdU; Invitrogen) and O-propargyl-puromycin (OPP; Invitrogen) incorporation assays were performed by intraperitoneal (IP) injection of pregnant dams with EdU (10mM, Invitrogen) or OPP (50 mg/kg, MedChemExpress) for 1 h prior to embryo collection. .. For tamoxifen labeling experiments, tamoxifen (Sigma, T5648) was prepared in 100% ethanol solution (20 mg/mL) and stored at -20oC.

    Article Title: TM4SF1 mediates YAP/TAZ regulation of coronary artery formation.
    Article Snippet: .. For cell proliferation analysis, pregnant mice were intraperitoneally injected with 5- ethynyl-2′-deoxyuridine (EdU; Life Technologies) at a dosage of 100 mg/kg. ..

    EdU Assay:

    Article Title: MARCH1 attenuates lung adenocarcinoma by blocking macrophage M2 polarization and cisplatin resistance through reducing SLC25A17 stability.
    Article Snippet: Cisplatin (DDP) resistance constitutes a major obstacle associated with poor prognosis in patients with lung adenocarcinoma (LUAD).. Membrane-associated RING-CH finger protein 1 (MARCH1) contributed to shaping an inflamed tumor microenvironment, which is linked to chemotherapy resistance in LUAD patients.. However, its role and underlying mechanisms in DDP-resistant LUAD patients remains to be elucidated.

    Staining:

    Article Title: Limitations of the p16-3MR mouse model for detecting and eliminating senescent cells.
    Article Snippet: .. For proliferation assays, MEFs were incubated with 2 μM 5-ethynyl-2′-deoxyuridine (EdU) for 3 days, fixed with 4% paraformaldehyde (PFA), and stained using the Click-iT EdU Cell Proliferation Kit for Imaging (C10640; Invitrogen). .. For cell viability assays, MEFs were treated with 10 μg/mL GCV or vehicle (Dimethyl sulfoxide (DMSO; 13445-74, Nacalai tesque)) for 6 days, © The Author(s) EMBO reports 11 with medium replaced every 2 days.

    Imaging:

    Article Title: Limitations of the p16-3MR mouse model for detecting and eliminating senescent cells.
    Article Snippet: .. For proliferation assays, MEFs were incubated with 2 μM 5-ethynyl-2′-deoxyuridine (EdU) for 3 days, fixed with 4% paraformaldehyde (PFA), and stained using the Click-iT EdU Cell Proliferation Kit for Imaging (C10640; Invitrogen). .. For cell viability assays, MEFs were treated with 10 μg/mL GCV or vehicle (Dimethyl sulfoxide (DMSO; 13445-74, Nacalai tesque)) for 6 days, © The Author(s) EMBO reports 11 with medium replaced every 2 days.

    Article Title: Transcriptional control of gemma dormancy via dual pathways in Marchantia polymorpha.
    Article Snippet: UN CO RR EC TE D MA NU SC RI PT



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    Cellular expression and functional validation of CTHRC1 in vitro . (A–C) Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analysis of SERPINE1, CTHRC1, and EGR1 in GES-1, AGS, HGC-27, and MKN-45 cells. (D) qRT-PCR screening of three CTHRC1-targeting short hairpin RNAs (shRNAs) in HGC-27 and AGS cells. (E) Western blot validation of CTHRC1 knockdown; glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was the loading control. (F, G) Cell Counting Kit-8 (CCK-8) proliferation assays. (H, I) Representative 5-ethynyl-2′-deoxyuridine (EdU) staining and quantification; scale bar, 100 µm. (J, K) Colony-formation assays and quantification; scale bar, 10 mm. (L–N) Wound-healing assays and migration-rate quantification; scale bar, 100 µm. (O–R) Transwell migration/invasion assays and quantification; scale bar, 100 µm. Data are mean ± standard deviation (SD) from three independent biological experiments. (A–D) were analyzed by one-way analysis of variance (ANOVA) with Dunnett correction, panels F and G by two-way ANOVA with multiple-comparison correction, and two-group endpoint comparisons by two-sided Student’s t-tests. ***P < 0.001.

    Journal: Frontiers in Immunology

    Article Title: A palmitoylation-associated three-gene signature predicts prognosis, immune features, and drug sensitivity in gastric cancer: functional validation of CTHRC1

    doi: 10.3389/fimmu.2026.1893438

    Figure Lengend Snippet: Cellular expression and functional validation of CTHRC1 in vitro . (A–C) Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analysis of SERPINE1, CTHRC1, and EGR1 in GES-1, AGS, HGC-27, and MKN-45 cells. (D) qRT-PCR screening of three CTHRC1-targeting short hairpin RNAs (shRNAs) in HGC-27 and AGS cells. (E) Western blot validation of CTHRC1 knockdown; glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was the loading control. (F, G) Cell Counting Kit-8 (CCK-8) proliferation assays. (H, I) Representative 5-ethynyl-2′-deoxyuridine (EdU) staining and quantification; scale bar, 100 µm. (J, K) Colony-formation assays and quantification; scale bar, 10 mm. (L–N) Wound-healing assays and migration-rate quantification; scale bar, 100 µm. (O–R) Transwell migration/invasion assays and quantification; scale bar, 100 µm. Data are mean ± standard deviation (SD) from three independent biological experiments. (A–D) were analyzed by one-way analysis of variance (ANOVA) with Dunnett correction, panels F and G by two-way ANOVA with multiple-comparison correction, and two-group endpoint comparisons by two-sided Student’s t-tests. ***P < 0.001.

    Article Snippet: DNA synthesis was assessed using a BeyoClick 5-ethynyl-2′-deoxyuridine (EdU)-594 Cell Proliferation Kit (Beyotime, C0078S).

    Techniques: Expressing, Functional Assay, Biomarker Discovery, In Vitro, Reverse Transcription, Polymerase Chain Reaction, Quantitative RT-PCR, Analysis, Screening, Western Blot, Knockdown, Control, Cell Counting, CCK-8 Assay, Staining, Colony Assay, Migration, Standard Deviation, Comparison