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e2  (MedChemExpress)


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    Structured Review

    MedChemExpress e2
    E2, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 198 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/estradiol+e2/Estradiol/pmc13253214-55-4-8
    Average 97 stars, based on 198 article reviews
    e2 - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Control:

    Article Title: PPP4C: a potential molecular marker and therapeutic target in thyroid cancer and triple-negative breast cancer.
    Article Snippet: For hormone treatment experiments, estradiol (E2) and liothyronine (T3) were purchased from MCE (China).. Stock solutions were prepared in dimethyl sulfoxide (DMSO) and stored at − 20°C.Stock solutions were prepared in dimethyl sulfoxide (DMSO) and stored at − 20°C.

    Article Title: Estradiol Reverses Ovariectomy-Induced Small RNA-mRNA Stress Signatures to Restore Neuroendocrine, Synaptic, and Immune Homeostasis in the Hypothalamus.
    Article Snippet: Estradiol (E2) was purchased from MedChemExpress (Nanjing, China) and dissolved in sesame oil.. Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [31].Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [31].

    Article Title: Estradiol Reverses Ovariectomy-Induced Small RNA–mRNA Stress Signatures to Restore Neuroendocrine, Synaptic, and Immune Homeostasis in the Hypothalamus
    Article Snippet: Estradiol (E2) was purchased from MedChemExpress (Nanjing, China) and dissolved in sesame oil.. Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [ ].Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [ ].

    Article Title: A novel functional assay based on patient-derived endometrial organoids and blastoids predicts the success of embryo transfer
    Article Snippet: On Day 4, 14 and 18 random wells were assigned for treatment with DMSO and hormones, respectively.On Day 4, 14 and 18 random wells were assigned for treatment with DMSO and hormones, respectively.. Medium in hormone-assigned wells were changed into hEMO medium supplemented with 10 nM Estradiol/E2 (MCE) on Day 4-5, followed by hEMO medium supplemented with EPCX (10 nM E2, 1 μM progesterone (MCE), 250 μM 8-bromo-cAMP (MCE), and 2 μM XAV-939) on Day 6-7.. Medium in control-assigned wells were replaced with hEMO medium supplemented with DMSO.Medium in control-assigned wells were replaced with hEMO medium supplemented with DMSO.

    Article Title: Exposure to responsible components of PM2.5 induces hepatic steatosis: An in silico, in vitro, and in vivo study based on adverse outcome pathway
    Article Snippet: DH5α-competent cells were purchased from Sangon Biotechnology (Shanghai, China).DH5α-competent cells were purchased from Sangon Biotechnology (Shanghai, China).. Estradiol (E2), Rosiglitazone (Rosi), GW9578, and GW3965 were purchased from MedChemExpress (Monmouth Junction, USA).. The human ERα, PPARα, PPARγ, and LXRα overexpressing vector and luciferase response element plasmid pERE-AUGLuc+ (ERα), pGL3-PPRE-Luc, pRP-LXRE-Luc were purchased from VectorBuilder (Guangzhou, China).The human ERα, PPARα, PPARγ, and LXRα overexpressing vector and luciferase response element plasmid pERE-AUGLuc+ (ERα), pGL3-PPRE-Luc, pRP-LXRE-Luc were purchased from VectorBuilder (Guangzhou, China).

    Article Title: Harnessing artificial intelligence to identify Bufalin as a molecular glue degrader of estrogen receptor alpha
    Article Snippet: Bufalin was purchased from APExBIO (USA).Bufalin was purchased from APExBIO (USA).. Fulvestrant, Estradiol (E2), and MG-132 were purchased from MedChemExpress (Monmouth Junction, NJ, USA).. MLN4924 was purchased from TargetMol (Washington, USA).MLN4924 was purchased from TargetMol (Washington, USA).

    Article Title: Harnessing artificial intelligence to identify Bufalin as a molecular glue degrader of estrogen receptor alpha.
    Article Snippet: was purchased from APExBIO (USA). .. Fulvestrant, Estradiol (E2), and MG-132 were purchased from MedChemExpress (Monmouth Junction, NJ, USA). .. MLN4924 was purchased from TargetMol (Was

    Article Title: TRIM24 as a therapeutic target in endocrine treatment–resistant breast cancer
    Article Snippet: free DMEM (Gibco) supplemented with 5% dextran/charcoal-stripped FBS (DCC medium) and 1% penicillin–streptomycin (5,000 U/mL, Life Technologies). .. For ligand treatment, 10 nM estradiol (E2) (HY-B0141; MedChemExpress) was used. .. TRIM24 degrader (dTRIM24; HY-111519 MedChemExpress) was reconstituted to a 10 mM stock solution, in DMSO and used in the described concentrations and

    Expressing:

    Article Title: PPP4C: a potential molecular marker and therapeutic target in thyroid cancer and triple-negative breast cancer.
    Article Snippet: For hormone treatment experiments, estradiol (E2) and liothyronine (T3) were purchased from MCE (China).. Stock solutions were prepared in dimethyl sulfoxide (DMSO) and stored at − 20°C.Stock solutions were prepared in dimethyl sulfoxide (DMSO) and stored at − 20°C.

    Article Title: Estradiol Reverses Ovariectomy-Induced Small RNA-mRNA Stress Signatures to Restore Neuroendocrine, Synaptic, and Immune Homeostasis in the Hypothalamus.
    Article Snippet: Estradiol (E2) was purchased from MedChemExpress (Nanjing, China) and dissolved in sesame oil.. Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [31].Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [31].

    Article Title: Estradiol Reverses Ovariectomy-Induced Small RNA–mRNA Stress Signatures to Restore Neuroendocrine, Synaptic, and Immune Homeostasis in the Hypothalamus
    Article Snippet: Estradiol (E2) was purchased from MedChemExpress (Nanjing, China) and dissolved in sesame oil.. Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [ ].Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [ ].

    Article Title: A novel functional assay based on patient-derived endometrial organoids and blastoids predicts the success of embryo transfer
    Article Snippet: On Day 4, 14 and 18 random wells were assigned for treatment with DMSO and hormones, respectively.On Day 4, 14 and 18 random wells were assigned for treatment with DMSO and hormones, respectively.. Medium in hormone-assigned wells were changed into hEMO medium supplemented with 10 nM Estradiol/E2 (MCE) on Day 4-5, followed by hEMO medium supplemented with EPCX (10 nM E2, 1 μM progesterone (MCE), 250 μM 8-bromo-cAMP (MCE), and 2 μM XAV-939) on Day 6-7.. Medium in control-assigned wells were replaced with hEMO medium supplemented with DMSO.Medium in control-assigned wells were replaced with hEMO medium supplemented with DMSO.

    Article Title: Exposure to responsible components of PM2.5 induces hepatic steatosis: An in silico, in vitro, and in vivo study based on adverse outcome pathway
    Article Snippet: DH5α-competent cells were purchased from Sangon Biotechnology (Shanghai, China).DH5α-competent cells were purchased from Sangon Biotechnology (Shanghai, China).. Estradiol (E2), Rosiglitazone (Rosi), GW9578, and GW3965 were purchased from MedChemExpress (Monmouth Junction, USA).. The human ERα, PPARα, PPARγ, and LXRα overexpressing vector and luciferase response element plasmid pERE-AUGLuc+ (ERα), pGL3-PPRE-Luc, pRP-LXRE-Luc were purchased from VectorBuilder (Guangzhou, China).The human ERα, PPARα, PPARγ, and LXRα overexpressing vector and luciferase response element plasmid pERE-AUGLuc+ (ERα), pGL3-PPRE-Luc, pRP-LXRE-Luc were purchased from VectorBuilder (Guangzhou, China).

    Article Title: Harnessing artificial intelligence to identify Bufalin as a molecular glue degrader of estrogen receptor alpha
    Article Snippet: Bufalin was purchased from APExBIO (USA).Bufalin was purchased from APExBIO (USA).. Fulvestrant, Estradiol (E2), and MG-132 were purchased from MedChemExpress (Monmouth Junction, NJ, USA).. MLN4924 was purchased from TargetMol (Washington, USA).MLN4924 was purchased from TargetMol (Washington, USA).

    Article Title: Harnessing artificial intelligence to identify Bufalin as a molecular glue degrader of estrogen receptor alpha.
    Article Snippet: was purchased from APExBIO (USA). .. Fulvestrant, Estradiol (E2), and MG-132 were purchased from MedChemExpress (Monmouth Junction, NJ, USA). .. MLN4924 was purchased from TargetMol (Was

    Article Title: TRIM24 as a therapeutic target in endocrine treatment–resistant breast cancer
    Article Snippet: free DMEM (Gibco) supplemented with 5% dextran/charcoal-stripped FBS (DCC medium) and 1% penicillin–streptomycin (5,000 U/mL, Life Technologies). .. For ligand treatment, 10 nM estradiol (E2) (HY-B0141; MedChemExpress) was used. .. TRIM24 degrader (dTRIM24; HY-111519 MedChemExpress) was reconstituted to a 10 mM stock solution, in DMSO and used in the described concentrations and

    Activity Assay:

    Article Title: PPP4C: a potential molecular marker and therapeutic target in thyroid cancer and triple-negative breast cancer.
    Article Snippet: For hormone treatment experiments, estradiol (E2) and liothyronine (T3) were purchased from MCE (China).. Stock solutions were prepared in dimethyl sulfoxide (DMSO) and stored at − 20°C.Stock solutions were prepared in dimethyl sulfoxide (DMSO) and stored at − 20°C.

    Article Title: Estradiol Reverses Ovariectomy-Induced Small RNA-mRNA Stress Signatures to Restore Neuroendocrine, Synaptic, and Immune Homeostasis in the Hypothalamus.
    Article Snippet: Estradiol (E2) was purchased from MedChemExpress (Nanjing, China) and dissolved in sesame oil.. Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [31].Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [31].

    Article Title: Estradiol Reverses Ovariectomy-Induced Small RNA–mRNA Stress Signatures to Restore Neuroendocrine, Synaptic, and Immune Homeostasis in the Hypothalamus
    Article Snippet: Estradiol (E2) was purchased from MedChemExpress (Nanjing, China) and dissolved in sesame oil.. Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [ ].Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [ ].

    Article Title: A novel functional assay based on patient-derived endometrial organoids and blastoids predicts the success of embryo transfer
    Article Snippet: On Day 4, 14 and 18 random wells were assigned for treatment with DMSO and hormones, respectively.On Day 4, 14 and 18 random wells were assigned for treatment with DMSO and hormones, respectively.. Medium in hormone-assigned wells were changed into hEMO medium supplemented with 10 nM Estradiol/E2 (MCE) on Day 4-5, followed by hEMO medium supplemented with EPCX (10 nM E2, 1 μM progesterone (MCE), 250 μM 8-bromo-cAMP (MCE), and 2 μM XAV-939) on Day 6-7.. Medium in control-assigned wells were replaced with hEMO medium supplemented with DMSO.Medium in control-assigned wells were replaced with hEMO medium supplemented with DMSO.

    Article Title: Exposure to responsible components of PM2.5 induces hepatic steatosis: An in silico, in vitro, and in vivo study based on adverse outcome pathway
    Article Snippet: DH5α-competent cells were purchased from Sangon Biotechnology (Shanghai, China).DH5α-competent cells were purchased from Sangon Biotechnology (Shanghai, China).. Estradiol (E2), Rosiglitazone (Rosi), GW9578, and GW3965 were purchased from MedChemExpress (Monmouth Junction, USA).. The human ERα, PPARα, PPARγ, and LXRα overexpressing vector and luciferase response element plasmid pERE-AUGLuc+ (ERα), pGL3-PPRE-Luc, pRP-LXRE-Luc were purchased from VectorBuilder (Guangzhou, China).The human ERα, PPARα, PPARγ, and LXRα overexpressing vector and luciferase response element plasmid pERE-AUGLuc+ (ERα), pGL3-PPRE-Luc, pRP-LXRE-Luc were purchased from VectorBuilder (Guangzhou, China).

    Article Title: Harnessing artificial intelligence to identify Bufalin as a molecular glue degrader of estrogen receptor alpha
    Article Snippet: Bufalin was purchased from APExBIO (USA).Bufalin was purchased from APExBIO (USA).. Fulvestrant, Estradiol (E2), and MG-132 were purchased from MedChemExpress (Monmouth Junction, NJ, USA).. MLN4924 was purchased from TargetMol (Washington, USA).MLN4924 was purchased from TargetMol (Washington, USA).

    Article Title: Harnessing artificial intelligence to identify Bufalin as a molecular glue degrader of estrogen receptor alpha.
    Article Snippet: was purchased from APExBIO (USA). .. Fulvestrant, Estradiol (E2), and MG-132 were purchased from MedChemExpress (Monmouth Junction, NJ, USA). .. MLN4924 was purchased from TargetMol (Was

    Article Title: TRIM24 as a therapeutic target in endocrine treatment–resistant breast cancer
    Article Snippet: free DMEM (Gibco) supplemented with 5% dextran/charcoal-stripped FBS (DCC medium) and 1% penicillin–streptomycin (5,000 U/mL, Life Technologies). .. For ligand treatment, 10 nM estradiol (E2) (HY-B0141; MedChemExpress) was used. .. TRIM24 degrader (dTRIM24; HY-111519 MedChemExpress) was reconstituted to a 10 mM stock solution, in DMSO and used in the described concentrations and

    Software:

    Article Title: PPP4C: a potential molecular marker and therapeutic target in thyroid cancer and triple-negative breast cancer.
    Article Snippet: For hormone treatment experiments, estradiol (E2) and liothyronine (T3) were purchased from MCE (China).. Stock solutions were prepared in dimethyl sulfoxide (DMSO) and stored at − 20°C.Stock solutions were prepared in dimethyl sulfoxide (DMSO) and stored at − 20°C.

    Article Title: Estradiol Reverses Ovariectomy-Induced Small RNA-mRNA Stress Signatures to Restore Neuroendocrine, Synaptic, and Immune Homeostasis in the Hypothalamus.
    Article Snippet: Estradiol (E2) was purchased from MedChemExpress (Nanjing, China) and dissolved in sesame oil.. Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [31].Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [31].

    Article Title: Estradiol Reverses Ovariectomy-Induced Small RNA–mRNA Stress Signatures to Restore Neuroendocrine, Synaptic, and Immune Homeostasis in the Hypothalamus
    Article Snippet: Estradiol (E2) was purchased from MedChemExpress (Nanjing, China) and dissolved in sesame oil.. Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [ ].Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [ ].

    Article Title: A novel functional assay based on patient-derived endometrial organoids and blastoids predicts the success of embryo transfer
    Article Snippet: On Day 4, 14 and 18 random wells were assigned for treatment with DMSO and hormones, respectively.On Day 4, 14 and 18 random wells were assigned for treatment with DMSO and hormones, respectively.. Medium in hormone-assigned wells were changed into hEMO medium supplemented with 10 nM Estradiol/E2 (MCE) on Day 4-5, followed by hEMO medium supplemented with EPCX (10 nM E2, 1 μM progesterone (MCE), 250 μM 8-bromo-cAMP (MCE), and 2 μM XAV-939) on Day 6-7.. Medium in control-assigned wells were replaced with hEMO medium supplemented with DMSO.Medium in control-assigned wells were replaced with hEMO medium supplemented with DMSO.

    Article Title: Exposure to responsible components of PM2.5 induces hepatic steatosis: An in silico, in vitro, and in vivo study based on adverse outcome pathway
    Article Snippet: DH5α-competent cells were purchased from Sangon Biotechnology (Shanghai, China).DH5α-competent cells were purchased from Sangon Biotechnology (Shanghai, China).. Estradiol (E2), Rosiglitazone (Rosi), GW9578, and GW3965 were purchased from MedChemExpress (Monmouth Junction, USA).. The human ERα, PPARα, PPARγ, and LXRα overexpressing vector and luciferase response element plasmid pERE-AUGLuc+ (ERα), pGL3-PPRE-Luc, pRP-LXRE-Luc were purchased from VectorBuilder (Guangzhou, China).The human ERα, PPARα, PPARγ, and LXRα overexpressing vector and luciferase response element plasmid pERE-AUGLuc+ (ERα), pGL3-PPRE-Luc, pRP-LXRE-Luc were purchased from VectorBuilder (Guangzhou, China).

    Article Title: Harnessing artificial intelligence to identify Bufalin as a molecular glue degrader of estrogen receptor alpha
    Article Snippet: Bufalin was purchased from APExBIO (USA).Bufalin was purchased from APExBIO (USA).. Fulvestrant, Estradiol (E2), and MG-132 were purchased from MedChemExpress (Monmouth Junction, NJ, USA).. MLN4924 was purchased from TargetMol (Washington, USA).MLN4924 was purchased from TargetMol (Washington, USA).

    Article Title: Harnessing artificial intelligence to identify Bufalin as a molecular glue degrader of estrogen receptor alpha.
    Article Snippet: was purchased from APExBIO (USA). .. Fulvestrant, Estradiol (E2), and MG-132 were purchased from MedChemExpress (Monmouth Junction, NJ, USA). .. MLN4924 was purchased from TargetMol (Was

    Article Title: TRIM24 as a therapeutic target in endocrine treatment–resistant breast cancer
    Article Snippet: free DMEM (Gibco) supplemented with 5% dextran/charcoal-stripped FBS (DCC medium) and 1% penicillin–streptomycin (5,000 U/mL, Life Technologies). .. For ligand treatment, 10 nM estradiol (E2) (HY-B0141; MedChemExpress) was used. .. TRIM24 degrader (dTRIM24; HY-111519 MedChemExpress) was reconstituted to a 10 mM stock solution, in DMSO and used in the described concentrations and

    Quantitative Proteomics:

    Article Title: PPP4C: a potential molecular marker and therapeutic target in thyroid cancer and triple-negative breast cancer.
    Article Snippet: For hormone treatment experiments, estradiol (E2) and liothyronine (T3) were purchased from MCE (China).. Stock solutions were prepared in dimethyl sulfoxide (DMSO) and stored at − 20°C.Stock solutions were prepared in dimethyl sulfoxide (DMSO) and stored at − 20°C.

    Article Title: Estradiol Reverses Ovariectomy-Induced Small RNA-mRNA Stress Signatures to Restore Neuroendocrine, Synaptic, and Immune Homeostasis in the Hypothalamus.
    Article Snippet: Estradiol (E2) was purchased from MedChemExpress (Nanjing, China) and dissolved in sesame oil.. Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [31].Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [31].

    Article Title: Estradiol Reverses Ovariectomy-Induced Small RNA–mRNA Stress Signatures to Restore Neuroendocrine, Synaptic, and Immune Homeostasis in the Hypothalamus
    Article Snippet: Estradiol (E2) was purchased from MedChemExpress (Nanjing, China) and dissolved in sesame oil.. Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [ ].Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [ ].

    Article Title: A novel functional assay based on patient-derived endometrial organoids and blastoids predicts the success of embryo transfer
    Article Snippet: On Day 4, 14 and 18 random wells were assigned for treatment with DMSO and hormones, respectively.On Day 4, 14 and 18 random wells were assigned for treatment with DMSO and hormones, respectively.. Medium in hormone-assigned wells were changed into hEMO medium supplemented with 10 nM Estradiol/E2 (MCE) on Day 4-5, followed by hEMO medium supplemented with EPCX (10 nM E2, 1 μM progesterone (MCE), 250 μM 8-bromo-cAMP (MCE), and 2 μM XAV-939) on Day 6-7.. Medium in control-assigned wells were replaced with hEMO medium supplemented with DMSO.Medium in control-assigned wells were replaced with hEMO medium supplemented with DMSO.

    Article Title: Exposure to responsible components of PM2.5 induces hepatic steatosis: An in silico, in vitro, and in vivo study based on adverse outcome pathway
    Article Snippet: DH5α-competent cells were purchased from Sangon Biotechnology (Shanghai, China).DH5α-competent cells were purchased from Sangon Biotechnology (Shanghai, China).. Estradiol (E2), Rosiglitazone (Rosi), GW9578, and GW3965 were purchased from MedChemExpress (Monmouth Junction, USA).. The human ERα, PPARα, PPARγ, and LXRα overexpressing vector and luciferase response element plasmid pERE-AUGLuc+ (ERα), pGL3-PPRE-Luc, pRP-LXRE-Luc were purchased from VectorBuilder (Guangzhou, China).The human ERα, PPARα, PPARγ, and LXRα overexpressing vector and luciferase response element plasmid pERE-AUGLuc+ (ERα), pGL3-PPRE-Luc, pRP-LXRE-Luc were purchased from VectorBuilder (Guangzhou, China).

    Article Title: Harnessing artificial intelligence to identify Bufalin as a molecular glue degrader of estrogen receptor alpha
    Article Snippet: Bufalin was purchased from APExBIO (USA).Bufalin was purchased from APExBIO (USA).. Fulvestrant, Estradiol (E2), and MG-132 were purchased from MedChemExpress (Monmouth Junction, NJ, USA).. MLN4924 was purchased from TargetMol (Washington, USA).MLN4924 was purchased from TargetMol (Washington, USA).

    Article Title: Harnessing artificial intelligence to identify Bufalin as a molecular glue degrader of estrogen receptor alpha.
    Article Snippet: was purchased from APExBIO (USA). .. Fulvestrant, Estradiol (E2), and MG-132 were purchased from MedChemExpress (Monmouth Junction, NJ, USA). .. MLN4924 was purchased from TargetMol (Was

    Article Title: TRIM24 as a therapeutic target in endocrine treatment–resistant breast cancer
    Article Snippet: free DMEM (Gibco) supplemented with 5% dextran/charcoal-stripped FBS (DCC medium) and 1% penicillin–streptomycin (5,000 U/mL, Life Technologies). .. For ligand treatment, 10 nM estradiol (E2) (HY-B0141; MedChemExpress) was used. .. TRIM24 degrader (dTRIM24; HY-111519 MedChemExpress) was reconstituted to a 10 mM stock solution, in DMSO and used in the described concentrations and

    Comparison:

    Article Title: PPP4C: a potential molecular marker and therapeutic target in thyroid cancer and triple-negative breast cancer.
    Article Snippet: For hormone treatment experiments, estradiol (E2) and liothyronine (T3) were purchased from MCE (China).. Stock solutions were prepared in dimethyl sulfoxide (DMSO) and stored at − 20°C.Stock solutions were prepared in dimethyl sulfoxide (DMSO) and stored at − 20°C.

    Article Title: Estradiol Reverses Ovariectomy-Induced Small RNA-mRNA Stress Signatures to Restore Neuroendocrine, Synaptic, and Immune Homeostasis in the Hypothalamus.
    Article Snippet: Estradiol (E2) was purchased from MedChemExpress (Nanjing, China) and dissolved in sesame oil.. Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [31].Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [31].

    Article Title: Estradiol Reverses Ovariectomy-Induced Small RNA–mRNA Stress Signatures to Restore Neuroendocrine, Synaptic, and Immune Homeostasis in the Hypothalamus
    Article Snippet: Estradiol (E2) was purchased from MedChemExpress (Nanjing, China) and dissolved in sesame oil.. Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [ ].Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [ ].

    Article Title: A novel functional assay based on patient-derived endometrial organoids and blastoids predicts the success of embryo transfer
    Article Snippet: On Day 4, 14 and 18 random wells were assigned for treatment with DMSO and hormones, respectively.On Day 4, 14 and 18 random wells were assigned for treatment with DMSO and hormones, respectively.. Medium in hormone-assigned wells were changed into hEMO medium supplemented with 10 nM Estradiol/E2 (MCE) on Day 4-5, followed by hEMO medium supplemented with EPCX (10 nM E2, 1 μM progesterone (MCE), 250 μM 8-bromo-cAMP (MCE), and 2 μM XAV-939) on Day 6-7.. Medium in control-assigned wells were replaced with hEMO medium supplemented with DMSO.Medium in control-assigned wells were replaced with hEMO medium supplemented with DMSO.

    Article Title: Exposure to responsible components of PM2.5 induces hepatic steatosis: An in silico, in vitro, and in vivo study based on adverse outcome pathway
    Article Snippet: DH5α-competent cells were purchased from Sangon Biotechnology (Shanghai, China).DH5α-competent cells were purchased from Sangon Biotechnology (Shanghai, China).. Estradiol (E2), Rosiglitazone (Rosi), GW9578, and GW3965 were purchased from MedChemExpress (Monmouth Junction, USA).. The human ERα, PPARα, PPARγ, and LXRα overexpressing vector and luciferase response element plasmid pERE-AUGLuc+ (ERα), pGL3-PPRE-Luc, pRP-LXRE-Luc were purchased from VectorBuilder (Guangzhou, China).The human ERα, PPARα, PPARγ, and LXRα overexpressing vector and luciferase response element plasmid pERE-AUGLuc+ (ERα), pGL3-PPRE-Luc, pRP-LXRE-Luc were purchased from VectorBuilder (Guangzhou, China).

    Article Title: Harnessing artificial intelligence to identify Bufalin as a molecular glue degrader of estrogen receptor alpha
    Article Snippet: Bufalin was purchased from APExBIO (USA).Bufalin was purchased from APExBIO (USA).. Fulvestrant, Estradiol (E2), and MG-132 were purchased from MedChemExpress (Monmouth Junction, NJ, USA).. MLN4924 was purchased from TargetMol (Washington, USA).MLN4924 was purchased from TargetMol (Washington, USA).

    Article Title: Harnessing artificial intelligence to identify Bufalin as a molecular glue degrader of estrogen receptor alpha.
    Article Snippet: was purchased from APExBIO (USA). .. Fulvestrant, Estradiol (E2), and MG-132 were purchased from MedChemExpress (Monmouth Junction, NJ, USA). .. MLN4924 was purchased from TargetMol (Was

    Article Title: TRIM24 as a therapeutic target in endocrine treatment–resistant breast cancer
    Article Snippet: free DMEM (Gibco) supplemented with 5% dextran/charcoal-stripped FBS (DCC medium) and 1% penicillin–streptomycin (5,000 U/mL, Life Technologies). .. For ligand treatment, 10 nM estradiol (E2) (HY-B0141; MedChemExpress) was used. .. TRIM24 degrader (dTRIM24; HY-111519 MedChemExpress) was reconstituted to a 10 mM stock solution, in DMSO and used in the described concentrations and

    Quantitative RT-PCR:

    Article Title: PPP4C: a potential molecular marker and therapeutic target in thyroid cancer and triple-negative breast cancer.
    Article Snippet: For hormone treatment experiments, estradiol (E2) and liothyronine (T3) were purchased from MCE (China).. Stock solutions were prepared in dimethyl sulfoxide (DMSO) and stored at − 20°C.Stock solutions were prepared in dimethyl sulfoxide (DMSO) and stored at − 20°C.

    Article Title: Estradiol Reverses Ovariectomy-Induced Small RNA-mRNA Stress Signatures to Restore Neuroendocrine, Synaptic, and Immune Homeostasis in the Hypothalamus.
    Article Snippet: Estradiol (E2) was purchased from MedChemExpress (Nanjing, China) and dissolved in sesame oil.. Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [31].Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [31].

    Article Title: Estradiol Reverses Ovariectomy-Induced Small RNA–mRNA Stress Signatures to Restore Neuroendocrine, Synaptic, and Immune Homeostasis in the Hypothalamus
    Article Snippet: Estradiol (E2) was purchased from MedChemExpress (Nanjing, China) and dissolved in sesame oil.. Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [ ].Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [ ].

    Article Title: A novel functional assay based on patient-derived endometrial organoids and blastoids predicts the success of embryo transfer
    Article Snippet: On Day 4, 14 and 18 random wells were assigned for treatment with DMSO and hormones, respectively.On Day 4, 14 and 18 random wells were assigned for treatment with DMSO and hormones, respectively.. Medium in hormone-assigned wells were changed into hEMO medium supplemented with 10 nM Estradiol/E2 (MCE) on Day 4-5, followed by hEMO medium supplemented with EPCX (10 nM E2, 1 μM progesterone (MCE), 250 μM 8-bromo-cAMP (MCE), and 2 μM XAV-939) on Day 6-7.. Medium in control-assigned wells were replaced with hEMO medium supplemented with DMSO.Medium in control-assigned wells were replaced with hEMO medium supplemented with DMSO.

    Article Title: Exposure to responsible components of PM2.5 induces hepatic steatosis: An in silico, in vitro, and in vivo study based on adverse outcome pathway
    Article Snippet: DH5α-competent cells were purchased from Sangon Biotechnology (Shanghai, China).DH5α-competent cells were purchased from Sangon Biotechnology (Shanghai, China).. Estradiol (E2), Rosiglitazone (Rosi), GW9578, and GW3965 were purchased from MedChemExpress (Monmouth Junction, USA).. The human ERα, PPARα, PPARγ, and LXRα overexpressing vector and luciferase response element plasmid pERE-AUGLuc+ (ERα), pGL3-PPRE-Luc, pRP-LXRE-Luc were purchased from VectorBuilder (Guangzhou, China).The human ERα, PPARα, PPARγ, and LXRα overexpressing vector and luciferase response element plasmid pERE-AUGLuc+ (ERα), pGL3-PPRE-Luc, pRP-LXRE-Luc were purchased from VectorBuilder (Guangzhou, China).

    Article Title: Harnessing artificial intelligence to identify Bufalin as a molecular glue degrader of estrogen receptor alpha
    Article Snippet: Bufalin was purchased from APExBIO (USA).Bufalin was purchased from APExBIO (USA).. Fulvestrant, Estradiol (E2), and MG-132 were purchased from MedChemExpress (Monmouth Junction, NJ, USA).. MLN4924 was purchased from TargetMol (Washington, USA).MLN4924 was purchased from TargetMol (Washington, USA).

    Article Title: Harnessing artificial intelligence to identify Bufalin as a molecular glue degrader of estrogen receptor alpha.
    Article Snippet: was purchased from APExBIO (USA). .. Fulvestrant, Estradiol (E2), and MG-132 were purchased from MedChemExpress (Monmouth Junction, NJ, USA). .. MLN4924 was purchased from TargetMol (Was

    Article Title: TRIM24 as a therapeutic target in endocrine treatment–resistant breast cancer
    Article Snippet: free DMEM (Gibco) supplemented with 5% dextran/charcoal-stripped FBS (DCC medium) and 1% penicillin–streptomycin (5,000 U/mL, Life Technologies). .. For ligand treatment, 10 nM estradiol (E2) (HY-B0141; MedChemExpress) was used. .. TRIM24 degrader (dTRIM24; HY-111519 MedChemExpress) was reconstituted to a 10 mM stock solution, in DMSO and used in the described concentrations and

    Biomarker Discovery:

    Article Title: PPP4C: a potential molecular marker and therapeutic target in thyroid cancer and triple-negative breast cancer.
    Article Snippet: For hormone treatment experiments, estradiol (E2) and liothyronine (T3) were purchased from MCE (China).. Stock solutions were prepared in dimethyl sulfoxide (DMSO) and stored at − 20°C.Stock solutions were prepared in dimethyl sulfoxide (DMSO) and stored at − 20°C.

    Article Title: Estradiol Reverses Ovariectomy-Induced Small RNA-mRNA Stress Signatures to Restore Neuroendocrine, Synaptic, and Immune Homeostasis in the Hypothalamus.
    Article Snippet: Estradiol (E2) was purchased from MedChemExpress (Nanjing, China) and dissolved in sesame oil.. Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [31].Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [31].

    Article Title: Estradiol Reverses Ovariectomy-Induced Small RNA–mRNA Stress Signatures to Restore Neuroendocrine, Synaptic, and Immune Homeostasis in the Hypothalamus
    Article Snippet: Estradiol (E2) was purchased from MedChemExpress (Nanjing, China) and dissolved in sesame oil.. Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [ ].Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [ ].

    Article Title: A novel functional assay based on patient-derived endometrial organoids and blastoids predicts the success of embryo transfer
    Article Snippet: On Day 4, 14 and 18 random wells were assigned for treatment with DMSO and hormones, respectively.On Day 4, 14 and 18 random wells were assigned for treatment with DMSO and hormones, respectively.. Medium in hormone-assigned wells were changed into hEMO medium supplemented with 10 nM Estradiol/E2 (MCE) on Day 4-5, followed by hEMO medium supplemented with EPCX (10 nM E2, 1 μM progesterone (MCE), 250 μM 8-bromo-cAMP (MCE), and 2 μM XAV-939) on Day 6-7.. Medium in control-assigned wells were replaced with hEMO medium supplemented with DMSO.Medium in control-assigned wells were replaced with hEMO medium supplemented with DMSO.

    Article Title: Exposure to responsible components of PM2.5 induces hepatic steatosis: An in silico, in vitro, and in vivo study based on adverse outcome pathway
    Article Snippet: DH5α-competent cells were purchased from Sangon Biotechnology (Shanghai, China).DH5α-competent cells were purchased from Sangon Biotechnology (Shanghai, China).. Estradiol (E2), Rosiglitazone (Rosi), GW9578, and GW3965 were purchased from MedChemExpress (Monmouth Junction, USA).. The human ERα, PPARα, PPARγ, and LXRα overexpressing vector and luciferase response element plasmid pERE-AUGLuc+ (ERα), pGL3-PPRE-Luc, pRP-LXRE-Luc were purchased from VectorBuilder (Guangzhou, China).The human ERα, PPARα, PPARγ, and LXRα overexpressing vector and luciferase response element plasmid pERE-AUGLuc+ (ERα), pGL3-PPRE-Luc, pRP-LXRE-Luc were purchased from VectorBuilder (Guangzhou, China).

    Article Title: Harnessing artificial intelligence to identify Bufalin as a molecular glue degrader of estrogen receptor alpha
    Article Snippet: Bufalin was purchased from APExBIO (USA).Bufalin was purchased from APExBIO (USA).. Fulvestrant, Estradiol (E2), and MG-132 were purchased from MedChemExpress (Monmouth Junction, NJ, USA).. MLN4924 was purchased from TargetMol (Washington, USA).MLN4924 was purchased from TargetMol (Washington, USA).

    Article Title: Harnessing artificial intelligence to identify Bufalin as a molecular glue degrader of estrogen receptor alpha.
    Article Snippet: was purchased from APExBIO (USA). .. Fulvestrant, Estradiol (E2), and MG-132 were purchased from MedChemExpress (Monmouth Junction, NJ, USA). .. MLN4924 was purchased from TargetMol (Was

    Article Title: TRIM24 as a therapeutic target in endocrine treatment–resistant breast cancer
    Article Snippet: free DMEM (Gibco) supplemented with 5% dextran/charcoal-stripped FBS (DCC medium) and 1% penicillin–streptomycin (5,000 U/mL, Life Technologies). .. For ligand treatment, 10 nM estradiol (E2) (HY-B0141; MedChemExpress) was used. .. TRIM24 degrader (dTRIM24; HY-111519 MedChemExpress) was reconstituted to a 10 mM stock solution, in DMSO and used in the described concentrations and

    Binding Assay:

    Article Title: PPP4C: a potential molecular marker and therapeutic target in thyroid cancer and triple-negative breast cancer.
    Article Snippet: For hormone treatment experiments, estradiol (E2) and liothyronine (T3) were purchased from MCE (China).. Stock solutions were prepared in dimethyl sulfoxide (DMSO) and stored at − 20°C.Stock solutions were prepared in dimethyl sulfoxide (DMSO) and stored at − 20°C.

    Article Title: Estradiol Reverses Ovariectomy-Induced Small RNA-mRNA Stress Signatures to Restore Neuroendocrine, Synaptic, and Immune Homeostasis in the Hypothalamus.
    Article Snippet: Estradiol (E2) was purchased from MedChemExpress (Nanjing, China) and dissolved in sesame oil.. Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [31].Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [31].

    Article Title: Estradiol Reverses Ovariectomy-Induced Small RNA–mRNA Stress Signatures to Restore Neuroendocrine, Synaptic, and Immune Homeostasis in the Hypothalamus
    Article Snippet: Estradiol (E2) was purchased from MedChemExpress (Nanjing, China) and dissolved in sesame oil.. Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [ ].Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [ ].

    Article Title: A novel functional assay based on patient-derived endometrial organoids and blastoids predicts the success of embryo transfer
    Article Snippet: On Day 4, 14 and 18 random wells were assigned for treatment with DMSO and hormones, respectively.On Day 4, 14 and 18 random wells were assigned for treatment with DMSO and hormones, respectively.. Medium in hormone-assigned wells were changed into hEMO medium supplemented with 10 nM Estradiol/E2 (MCE) on Day 4-5, followed by hEMO medium supplemented with EPCX (10 nM E2, 1 μM progesterone (MCE), 250 μM 8-bromo-cAMP (MCE), and 2 μM XAV-939) on Day 6-7.. Medium in control-assigned wells were replaced with hEMO medium supplemented with DMSO.Medium in control-assigned wells were replaced with hEMO medium supplemented with DMSO.

    Article Title: Exposure to responsible components of PM2.5 induces hepatic steatosis: An in silico, in vitro, and in vivo study based on adverse outcome pathway
    Article Snippet: DH5α-competent cells were purchased from Sangon Biotechnology (Shanghai, China).DH5α-competent cells were purchased from Sangon Biotechnology (Shanghai, China).. Estradiol (E2), Rosiglitazone (Rosi), GW9578, and GW3965 were purchased from MedChemExpress (Monmouth Junction, USA).. The human ERα, PPARα, PPARγ, and LXRα overexpressing vector and luciferase response element plasmid pERE-AUGLuc+ (ERα), pGL3-PPRE-Luc, pRP-LXRE-Luc were purchased from VectorBuilder (Guangzhou, China).The human ERα, PPARα, PPARγ, and LXRα overexpressing vector and luciferase response element plasmid pERE-AUGLuc+ (ERα), pGL3-PPRE-Luc, pRP-LXRE-Luc were purchased from VectorBuilder (Guangzhou, China).

    Article Title: Harnessing artificial intelligence to identify Bufalin as a molecular glue degrader of estrogen receptor alpha
    Article Snippet: Bufalin was purchased from APExBIO (USA).Bufalin was purchased from APExBIO (USA).. Fulvestrant, Estradiol (E2), and MG-132 were purchased from MedChemExpress (Monmouth Junction, NJ, USA).. MLN4924 was purchased from TargetMol (Washington, USA).MLN4924 was purchased from TargetMol (Washington, USA).

    Article Title: Harnessing artificial intelligence to identify Bufalin as a molecular glue degrader of estrogen receptor alpha.
    Article Snippet: was purchased from APExBIO (USA). .. Fulvestrant, Estradiol (E2), and MG-132 were purchased from MedChemExpress (Monmouth Junction, NJ, USA). .. MLN4924 was purchased from TargetMol (Was

    Article Title: TRIM24 as a therapeutic target in endocrine treatment–resistant breast cancer
    Article Snippet: free DMEM (Gibco) supplemented with 5% dextran/charcoal-stripped FBS (DCC medium) and 1% penicillin–streptomycin (5,000 U/mL, Life Technologies). .. For ligand treatment, 10 nM estradiol (E2) (HY-B0141; MedChemExpress) was used. .. TRIM24 degrader (dTRIM24; HY-111519 MedChemExpress) was reconstituted to a 10 mM stock solution, in DMSO and used in the described concentrations and

    ChIP-sequencing:

    Article Title: PPP4C: a potential molecular marker and therapeutic target in thyroid cancer and triple-negative breast cancer.
    Article Snippet: For hormone treatment experiments, estradiol (E2) and liothyronine (T3) were purchased from MCE (China).. Stock solutions were prepared in dimethyl sulfoxide (DMSO) and stored at − 20°C.Stock solutions were prepared in dimethyl sulfoxide (DMSO) and stored at − 20°C.

    Article Title: Estradiol Reverses Ovariectomy-Induced Small RNA-mRNA Stress Signatures to Restore Neuroendocrine, Synaptic, and Immune Homeostasis in the Hypothalamus.
    Article Snippet: Estradiol (E2) was purchased from MedChemExpress (Nanjing, China) and dissolved in sesame oil.. Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [31].Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [31].

    Article Title: Estradiol Reverses Ovariectomy-Induced Small RNA–mRNA Stress Signatures to Restore Neuroendocrine, Synaptic, and Immune Homeostasis in the Hypothalamus
    Article Snippet: Estradiol (E2) was purchased from MedChemExpress (Nanjing, China) and dissolved in sesame oil.. Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [ ].Mice in the OVX + E2 and E2 groups received daily intraperitoneal injections of estradiol (0.2 mg/kg body weight) for four weeks, following a previously established protocol [ ].

    Article Title: A novel functional assay based on patient-derived endometrial organoids and blastoids predicts the success of embryo transfer
    Article Snippet: On Day 4, 14 and 18 random wells were assigned for treatment with DMSO and hormones, respectively.On Day 4, 14 and 18 random wells were assigned for treatment with DMSO and hormones, respectively.. Medium in hormone-assigned wells were changed into hEMO medium supplemented with 10 nM Estradiol/E2 (MCE) on Day 4-5, followed by hEMO medium supplemented with EPCX (10 nM E2, 1 μM progesterone (MCE), 250 μM 8-bromo-cAMP (MCE), and 2 μM XAV-939) on Day 6-7.. Medium in control-assigned wells were replaced with hEMO medium supplemented with DMSO.Medium in control-assigned wells were replaced with hEMO medium supplemented with DMSO.

    Article Title: Exposure to responsible components of PM2.5 induces hepatic steatosis: An in silico, in vitro, and in vivo study based on adverse outcome pathway
    Article Snippet: DH5α-competent cells were purchased from Sangon Biotechnology (Shanghai, China).DH5α-competent cells were purchased from Sangon Biotechnology (Shanghai, China).. Estradiol (E2), Rosiglitazone (Rosi), GW9578, and GW3965 were purchased from MedChemExpress (Monmouth Junction, USA).. The human ERα, PPARα, PPARγ, and LXRα overexpressing vector and luciferase response element plasmid pERE-AUGLuc+ (ERα), pGL3-PPRE-Luc, pRP-LXRE-Luc were purchased from VectorBuilder (Guangzhou, China).The human ERα, PPARα, PPARγ, and LXRα overexpressing vector and luciferase response element plasmid pERE-AUGLuc+ (ERα), pGL3-PPRE-Luc, pRP-LXRE-Luc were purchased from VectorBuilder (Guangzhou, China).

    Article Title: Harnessing artificial intelligence to identify Bufalin as a molecular glue degrader of estrogen receptor alpha
    Article Snippet: Bufalin was purchased from APExBIO (USA).Bufalin was purchased from APExBIO (USA).. Fulvestrant, Estradiol (E2), and MG-132 were purchased from MedChemExpress (Monmouth Junction, NJ, USA).. MLN4924 was purchased from TargetMol (Washington, USA).MLN4924 was purchased from TargetMol (Washington, USA).

    Article Title: Harnessing artificial intelligence to identify Bufalin as a molecular glue degrader of estrogen receptor alpha.
    Article Snippet: was purchased from APExBIO (USA). .. Fulvestrant, Estradiol (E2), and MG-132 were purchased from MedChemExpress (Monmouth Junction, NJ, USA). .. MLN4924 was purchased from TargetMol (Was

    Article Title: TRIM24 as a therapeutic target in endocrine treatment–resistant breast cancer
    Article Snippet: free DMEM (Gibco) supplemented with 5% dextran/charcoal-stripped FBS (DCC medium) and 1% penicillin–streptomycin (5,000 U/mL, Life Technologies). .. For ligand treatment, 10 nM estradiol (E2) (HY-B0141; MedChemExpress) was used. .. TRIM24 degrader (dTRIM24; HY-111519 MedChemExpress) was reconstituted to a 10 mM stock solution, in DMSO and used in the described concentrations and



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