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addgene plasmid  (Addgene inc)


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    Structured Review

    Addgene inc addgene plasmid
    Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 204 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/espcas9/eSpCas9(1%2E1)+(Plasmid+%2371814)/bio_rxiv__64898__2026__03__24__713622-222-20-20
    Average 96 stars, based on 204 article reviews
    addgene plasmid - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Transfection:

    Article Title: Deciphering the role of the lncRNA TRIBAL in hepatocyte models.
    Article Snippet: .. Cells were transfected with pCRU6 constructs and eSpCas9 a gift from Feng Zhang (Addgene plasmid # 71814) [20]. ..

    Article Title: Deciphering the role of the lncRNA TRIBAL in hepatocyte models
    Article Snippet: .. Cells were transfected with pCRU6 constructs and eSpCas9 a gift from Feng Zhang (Addgene plasmid # 71814) [ ]. ..

    Article Title: Deciphering the role of the lncRNA TRIBAL in hepatocyte models
    Article Snippet: .. Cells were transfected with pCRU6 constructs and eSpCas9 a gift from Feng Zhang (Addgene plasmid # 71814) [ ]. ..

    Construct:

    Article Title: Deciphering the role of the lncRNA TRIBAL in hepatocyte models.
    Article Snippet: .. Cells were transfected with pCRU6 constructs and eSpCas9 a gift from Feng Zhang (Addgene plasmid # 71814) [20]. ..

    Article Title: Deciphering the role of the lncRNA TRIBAL in hepatocyte models
    Article Snippet: .. Cells were transfected with pCRU6 constructs and eSpCas9 a gift from Feng Zhang (Addgene plasmid # 71814) [ ]. ..

    Article Title: Deciphering the role of the lncRNA TRIBAL in hepatocyte models
    Article Snippet: .. Cells were transfected with pCRU6 constructs and eSpCas9 a gift from Feng Zhang (Addgene plasmid # 71814) [ ]. ..

    Plasmid Preparation:

    Article Title: Deciphering the role of the lncRNA TRIBAL in hepatocyte models.
    Article Snippet: .. Cells were transfected with pCRU6 constructs and eSpCas9 a gift from Feng Zhang (Addgene plasmid # 71814) [20]. ..

    Article Title: Deciphering the role of the lncRNA TRIBAL in hepatocyte models
    Article Snippet: .. Cells were transfected with pCRU6 constructs and eSpCas9 a gift from Feng Zhang (Addgene plasmid # 71814) [ ]. ..

    Article Title: Effect of rapamycin on lysosomal accumulation in a CRISPR/Cas9-based cellular model of VPS13A deficiency.
    Article Snippet: The guide RNA expression plasmid for CRISPR/Cas9 was obtained by annealing of two complementary oligonucleotides (F: 5′- CACCG TTC TTG GGG GAC TATGTGG - 3′; R: 5′- AAACC CAC ATA GTC CCC CAAGAAC) and cloning in the BsmB1 site of the BPK1520 vector (Addgene, #65777). .. The plasmid for Cas9 expression was eSPCas9 (Addgene, #71814). .. TFEB_WT- MYC (Addgene, #99955).

    Article Title: Deciphering the role of the lncRNA TRIBAL in hepatocyte models
    Article Snippet: .. Cells were transfected with pCRU6 constructs and eSpCas9 a gift from Feng Zhang (Addgene plasmid # 71814) [ ]. ..

    Cloning:

    Article Title: Target-locking single-molecule nanoscopy
    Article Snippet: .. For gRNA cloning, oligo pairs containing partially complementary sequences were annealed and ligated into the BbsI site of espCas9 (Addgene 71814), resulting in espCas9-gRNA-Sox2, -Cdk9, -Brd4 and -Med22 respectively. ..

    Expressing:

    Article Title: Effect of rapamycin on lysosomal accumulation in a CRISPR/Cas9-based cellular model of VPS13A deficiency.
    Article Snippet: The guide RNA expression plasmid for CRISPR/Cas9 was obtained by annealing of two complementary oligonucleotides (F: 5′- CACCG TTC TTG GGG GAC TATGTGG - 3′; R: 5′- AAACC CAC ATA GTC CCC CAAGAAC) and cloning in the BsmB1 site of the BPK1520 vector (Addgene, #65777). .. The plasmid for Cas9 expression was eSPCas9 (Addgene, #71814). .. TFEB_WT- MYC (Addgene, #99955).

    Clone Assay:

    Article Title: A cytosolic surveillance mechanism activates the mitochondrial UPR.
    Article Snippet: .. The single guide RNAs (sgRNAs) were cloned into eSpCas9 (1.1; Addgene, catalogue no. 71814). ..



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