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human normal human epidermal keratinocytes nhek  (PromoCell)


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    PromoCell human normal human epidermal keratinocytes nhek
    UA beneficial impact on human <t>keratinocytes</t> and reconstituted human epidermis (A) Mitochondrial respiration (basal and maximal) assessed as oxygen consumption rate (OCR) in human dermal keratinocytes after 72 h of differentiation from young donor treated with DMSO for 24 h or the indicated dose of UA. ∗ p < 0.05 and ∗∗∗ p < 0.001 after one-way ANOVA. Error bars represent mean ± SEM. (B) TOMM20 immunostaining of skin epidermis in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as antibody staining signal over imaging field. ∗ p < 0.05 and ∗∗∗∗ p < 0.0001 after unpaired t test one-way ANOVA. Error bars represent mean ± SEM. Scale bars, 50 μm. (C) Relative gene expression of PINK1 and PARK2 in reconstructed human epidermis (RHE). RHE tissues were moisturized for 24 h with or without 1% Mitopure, and relative expression levels of PINK1 and PARK2 were quantified. Statistical analysis was performed using one-way ANOVA (∗ p < 0.05; ∗∗ p < 0.01). Error bars represent mean ± SEM. (D) Interleukin 6 levels normalized to cell viability in keratinocytes. Keratinocytes were stimulated with a pro-inflammatory cytokine mix for 24 h then treated for an additional 24 h with either DMSO or 1 μM UA. IL-6 secretion was quantified and normalized to cell viability. Statistical analysis was performed using one-way ANOVA (∗∗∗∗ p < 0.0001). Error bars represent mean ± SEM. (E) p16 immunostaining in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as antibody staining signal over imaging field. ∗∗∗∗ p < 0.0001 after unpaired t test. Error bars represent mean ± SEM. Scale bars, 50 μm. (F) Ki67 immunostaining in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as number of Ki67 positive cells normalized over μm of dermal epidermal junction (count/μm) ∗∗∗∗ p < 0.0001 after unpaired t test. Error bars represent mean ± SEM. Scale bars, 50 μm. (G) Keratin 10 immunostaining in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as fluorescence intensity over epidermis area. ∗∗∗∗ p < 0.0001 after unpaired t test. Error bars represent mean ± SEM. Scale bars, 50 μm.
    Human Normal Human Epidermal Keratinocytes Nhek, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/epidermal+keratinocytes/Normal+Human+Epidermal+Keratinocytes+(NHEK)+adult%2C+single+donor/pmc13657230-62-0-7
    Average 95 stars, based on 101 article reviews
    human normal human epidermal keratinocytes nhek - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Topical application of urolithin A activates mitophagy and enhances mitochondrial function to promote skin longevity"

    Article Title: Topical application of urolithin A activates mitophagy and enhances mitochondrial function to promote skin longevity

    Journal: iScience

    doi: 10.1016/j.isci.2026.117646

    UA beneficial impact on human keratinocytes and reconstituted human epidermis (A) Mitochondrial respiration (basal and maximal) assessed as oxygen consumption rate (OCR) in human dermal keratinocytes after 72 h of differentiation from young donor treated with DMSO for 24 h or the indicated dose of UA. ∗ p < 0.05 and ∗∗∗ p < 0.001 after one-way ANOVA. Error bars represent mean ± SEM. (B) TOMM20 immunostaining of skin epidermis in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as antibody staining signal over imaging field. ∗ p < 0.05 and ∗∗∗∗ p < 0.0001 after unpaired t test one-way ANOVA. Error bars represent mean ± SEM. Scale bars, 50 μm. (C) Relative gene expression of PINK1 and PARK2 in reconstructed human epidermis (RHE). RHE tissues were moisturized for 24 h with or without 1% Mitopure, and relative expression levels of PINK1 and PARK2 were quantified. Statistical analysis was performed using one-way ANOVA (∗ p < 0.05; ∗∗ p < 0.01). Error bars represent mean ± SEM. (D) Interleukin 6 levels normalized to cell viability in keratinocytes. Keratinocytes were stimulated with a pro-inflammatory cytokine mix for 24 h then treated for an additional 24 h with either DMSO or 1 μM UA. IL-6 secretion was quantified and normalized to cell viability. Statistical analysis was performed using one-way ANOVA (∗∗∗∗ p < 0.0001). Error bars represent mean ± SEM. (E) p16 immunostaining in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as antibody staining signal over imaging field. ∗∗∗∗ p < 0.0001 after unpaired t test. Error bars represent mean ± SEM. Scale bars, 50 μm. (F) Ki67 immunostaining in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as number of Ki67 positive cells normalized over μm of dermal epidermal junction (count/μm) ∗∗∗∗ p < 0.0001 after unpaired t test. Error bars represent mean ± SEM. Scale bars, 50 μm. (G) Keratin 10 immunostaining in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as fluorescence intensity over epidermis area. ∗∗∗∗ p < 0.0001 after unpaired t test. Error bars represent mean ± SEM. Scale bars, 50 μm.
    Figure Legend Snippet: UA beneficial impact on human keratinocytes and reconstituted human epidermis (A) Mitochondrial respiration (basal and maximal) assessed as oxygen consumption rate (OCR) in human dermal keratinocytes after 72 h of differentiation from young donor treated with DMSO for 24 h or the indicated dose of UA. ∗ p < 0.05 and ∗∗∗ p < 0.001 after one-way ANOVA. Error bars represent mean ± SEM. (B) TOMM20 immunostaining of skin epidermis in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as antibody staining signal over imaging field. ∗ p < 0.05 and ∗∗∗∗ p < 0.0001 after unpaired t test one-way ANOVA. Error bars represent mean ± SEM. Scale bars, 50 μm. (C) Relative gene expression of PINK1 and PARK2 in reconstructed human epidermis (RHE). RHE tissues were moisturized for 24 h with or without 1% Mitopure, and relative expression levels of PINK1 and PARK2 were quantified. Statistical analysis was performed using one-way ANOVA (∗ p < 0.05; ∗∗ p < 0.01). Error bars represent mean ± SEM. (D) Interleukin 6 levels normalized to cell viability in keratinocytes. Keratinocytes were stimulated with a pro-inflammatory cytokine mix for 24 h then treated for an additional 24 h with either DMSO or 1 μM UA. IL-6 secretion was quantified and normalized to cell viability. Statistical analysis was performed using one-way ANOVA (∗∗∗∗ p < 0.0001). Error bars represent mean ± SEM. (E) p16 immunostaining in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as antibody staining signal over imaging field. ∗∗∗∗ p < 0.0001 after unpaired t test. Error bars represent mean ± SEM. Scale bars, 50 μm. (F) Ki67 immunostaining in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as number of Ki67 positive cells normalized over μm of dermal epidermal junction (count/μm) ∗∗∗∗ p < 0.0001 after unpaired t test. Error bars represent mean ± SEM. Scale bars, 50 μm. (G) Keratin 10 immunostaining in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as fluorescence intensity over epidermis area. ∗∗∗∗ p < 0.0001 after unpaired t test. Error bars represent mean ± SEM. Scale bars, 50 μm.

    Techniques Used: Immunostaining, Staining, Imaging, Gene Expression, Expressing, Analysis, Fluorescence


    Figure Legend Snippet:

    Techniques Used: Binding Assay, Ex Vivo, Recombinant, Metals, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Cell Viability Assay, Staining, DC Protein Assay, Gene Expression, Glass, Software, Imaging, Microscopy, Real-time Polymerase Chain Reaction

    Related Articles

    Isolation:

    Article Title: Deimination is regulated at multiple levels including auto-deimination of peptidylarginine deiminases
    Article Snippet: .. Epidermal keratinocytes were isolated according to the procedure described in [ 40 ], and cultured in the defined KBM-2 medium (Promocell, Heidelberg, Germany) supplemented with several growth factors as follows: 0.4% bovine pituitary extract, 0.125 ng/ml epidermal growth factor, 5 μg/ml human insulin, 0.33 μg/ml hydrocortisone, 10 μg/ml human transferrin, 0.39 μg/ml epinephrine, and 0.15 mM CaCl 2 in the presence of 100 μg/ml primocin TM (Cayla-Invivogen, Toulouse, France) in a humidified incubator at 37°C with 5% CO 2 . ..

    Article Title: Deimination is regulated at multiple levels including auto-deimination of peptidylarginine deiminases.
    Article Snippet: Peptidylarginine deiminases (PADs) catalyze deimination, converting arginyl to citrullyl residues.. Only three PAD isotypes are detected in the epidermis where they play a crucial role, targeting filaggrin, a key actor for the tissue hydration and barrier functions.. Their expression and activation depends on the keratinocyte differentiation state.

    Cell Culture:

    Article Title: Deimination is regulated at multiple levels including auto-deimination of peptidylarginine deiminases
    Article Snippet: .. Epidermal keratinocytes were isolated according to the procedure described in [ 40 ], and cultured in the defined KBM-2 medium (Promocell, Heidelberg, Germany) supplemented with several growth factors as follows: 0.4% bovine pituitary extract, 0.125 ng/ml epidermal growth factor, 5 μg/ml human insulin, 0.33 μg/ml hydrocortisone, 10 μg/ml human transferrin, 0.39 μg/ml epinephrine, and 0.15 mM CaCl 2 in the presence of 100 μg/ml primocin TM (Cayla-Invivogen, Toulouse, France) in a humidified incubator at 37°C with 5% CO 2 . ..

    Article Title: Deimination is regulated at multiple levels including auto-deimination of peptidylarginine deiminases.
    Article Snippet: Peptidylarginine deiminases (PADs) catalyze deimination, converting arginyl to citrullyl residues.. Only three PAD isotypes are detected in the epidermis where they play a crucial role, targeting filaggrin, a key actor for the tissue hydration and barrier functions.. Their expression and activation depends on the keratinocyte differentiation state.

    Incubator:

    Article Title: Deimination is regulated at multiple levels including auto-deimination of peptidylarginine deiminases
    Article Snippet: .. Epidermal keratinocytes were isolated according to the procedure described in [ 40 ], and cultured in the defined KBM-2 medium (Promocell, Heidelberg, Germany) supplemented with several growth factors as follows: 0.4% bovine pituitary extract, 0.125 ng/ml epidermal growth factor, 5 μg/ml human insulin, 0.33 μg/ml hydrocortisone, 10 μg/ml human transferrin, 0.39 μg/ml epinephrine, and 0.15 mM CaCl 2 in the presence of 100 μg/ml primocin TM (Cayla-Invivogen, Toulouse, France) in a humidified incubator at 37°C with 5% CO 2 . ..

    Probiotics:

    Article Title: A survey of multiple candidate probiotic bacteria reveals specificity in the ability to modify the effects of key wound pathogens.
    Article Snippet: .. Testing the protective effect of lysates from candidate probiotics on the viability of human epidermal keratinocytes using flow cytometry Primary normal human epidermal keratinocytes (NHEK) were purchased from Promocell (Germany) and grown and maintained as described previously (21). ..

    Flow Cytometry:

    Article Title: A survey of multiple candidate probiotic bacteria reveals specificity in the ability to modify the effects of key wound pathogens.
    Article Snippet: .. Testing the protective effect of lysates from candidate probiotics on the viability of human epidermal keratinocytes using flow cytometry Primary normal human epidermal keratinocytes (NHEK) were purchased from Promocell (Germany) and grown and maintained as described previously (21). ..

    other:

    Article Title: Transgenic system for reversibly immortalizing mammalian quiescent cells
    Article Snippet: Doxycycline—Regulated Reversible Immortalization of Human Keratinocytes Replication Life Span Determinations Normal proliferating human epidermal keratinocytes from adult foreskin (ref.C 12004) and ready-to-use keratinocyte growth medium 2 (ref. 20211) were both purchased from PromoCell GmbH (Heidelberg, Germany).



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    CLS Cell Lines Service GmbH human epidermal keratinocyte
    UA beneficial impact on human <t>keratinocytes</t> and reconstituted human epidermis (A) Mitochondrial respiration (basal and maximal) assessed as oxygen consumption rate (OCR) in human dermal keratinocytes after 72 h of differentiation from young donor treated with DMSO for 24 h or the indicated dose of UA. ∗ p < 0.05 and ∗∗∗ p < 0.001 after one-way ANOVA. Error bars represent mean ± SEM. (B) TOMM20 immunostaining of skin epidermis in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as antibody staining signal over imaging field. ∗ p < 0.05 and ∗∗∗∗ p < 0.0001 after unpaired t test one-way ANOVA. Error bars represent mean ± SEM. Scale bars, 50 μm. (C) Relative gene expression of PINK1 and PARK2 in reconstructed human epidermis (RHE). RHE tissues were moisturized for 24 h with or without 1% Mitopure, and relative expression levels of PINK1 and PARK2 were quantified. Statistical analysis was performed using one-way ANOVA (∗ p < 0.05; ∗∗ p < 0.01). Error bars represent mean ± SEM. (D) Interleukin 6 levels normalized to cell viability in keratinocytes. Keratinocytes were stimulated with a pro-inflammatory cytokine mix for 24 h then treated for an additional 24 h with either DMSO or 1 μM UA. IL-6 secretion was quantified and normalized to cell viability. Statistical analysis was performed using one-way ANOVA (∗∗∗∗ p < 0.0001). Error bars represent mean ± SEM. (E) p16 immunostaining in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as antibody staining signal over imaging field. ∗∗∗∗ p < 0.0001 after unpaired t test. Error bars represent mean ± SEM. Scale bars, 50 μm. (F) Ki67 immunostaining in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as number of Ki67 positive cells normalized over μm of dermal epidermal junction (count/μm) ∗∗∗∗ p < 0.0001 after unpaired t test. Error bars represent mean ± SEM. Scale bars, 50 μm. (G) Keratin 10 immunostaining in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as fluorescence intensity over epidermis area. ∗∗∗∗ p < 0.0001 after unpaired t test. Error bars represent mean ± SEM. Scale bars, 50 μm.
    Human Epidermal Keratinocyte, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    normal human epidermal keratinocytes  (ATCC)
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    ATCC normal human epidermal keratinocytes
    UA beneficial impact on human <t>keratinocytes</t> and reconstituted human epidermis (A) Mitochondrial respiration (basal and maximal) assessed as oxygen consumption rate (OCR) in human dermal keratinocytes after 72 h of differentiation from young donor treated with DMSO for 24 h or the indicated dose of UA. ∗ p < 0.05 and ∗∗∗ p < 0.001 after one-way ANOVA. Error bars represent mean ± SEM. (B) TOMM20 immunostaining of skin epidermis in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as antibody staining signal over imaging field. ∗ p < 0.05 and ∗∗∗∗ p < 0.0001 after unpaired t test one-way ANOVA. Error bars represent mean ± SEM. Scale bars, 50 μm. (C) Relative gene expression of PINK1 and PARK2 in reconstructed human epidermis (RHE). RHE tissues were moisturized for 24 h with or without 1% Mitopure, and relative expression levels of PINK1 and PARK2 were quantified. Statistical analysis was performed using one-way ANOVA (∗ p < 0.05; ∗∗ p < 0.01). Error bars represent mean ± SEM. (D) Interleukin 6 levels normalized to cell viability in keratinocytes. Keratinocytes were stimulated with a pro-inflammatory cytokine mix for 24 h then treated for an additional 24 h with either DMSO or 1 μM UA. IL-6 secretion was quantified and normalized to cell viability. Statistical analysis was performed using one-way ANOVA (∗∗∗∗ p < 0.0001). Error bars represent mean ± SEM. (E) p16 immunostaining in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as antibody staining signal over imaging field. ∗∗∗∗ p < 0.0001 after unpaired t test. Error bars represent mean ± SEM. Scale bars, 50 μm. (F) Ki67 immunostaining in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as number of Ki67 positive cells normalized over μm of dermal epidermal junction (count/μm) ∗∗∗∗ p < 0.0001 after unpaired t test. Error bars represent mean ± SEM. Scale bars, 50 μm. (G) Keratin 10 immunostaining in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as fluorescence intensity over epidermis area. ∗∗∗∗ p < 0.0001 after unpaired t test. Error bars represent mean ± SEM. Scale bars, 50 μm.
    Normal Human Epidermal Keratinocytes, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nheks  (ATCC)
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    ATCC nheks
    UA beneficial impact on human <t>keratinocytes</t> and reconstituted human epidermis (A) Mitochondrial respiration (basal and maximal) assessed as oxygen consumption rate (OCR) in human dermal keratinocytes after 72 h of differentiation from young donor treated with DMSO for 24 h or the indicated dose of UA. ∗ p < 0.05 and ∗∗∗ p < 0.001 after one-way ANOVA. Error bars represent mean ± SEM. (B) TOMM20 immunostaining of skin epidermis in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as antibody staining signal over imaging field. ∗ p < 0.05 and ∗∗∗∗ p < 0.0001 after unpaired t test one-way ANOVA. Error bars represent mean ± SEM. Scale bars, 50 μm. (C) Relative gene expression of PINK1 and PARK2 in reconstructed human epidermis (RHE). RHE tissues were moisturized for 24 h with or without 1% Mitopure, and relative expression levels of PINK1 and PARK2 were quantified. Statistical analysis was performed using one-way ANOVA (∗ p < 0.05; ∗∗ p < 0.01). Error bars represent mean ± SEM. (D) Interleukin 6 levels normalized to cell viability in keratinocytes. Keratinocytes were stimulated with a pro-inflammatory cytokine mix for 24 h then treated for an additional 24 h with either DMSO or 1 μM UA. IL-6 secretion was quantified and normalized to cell viability. Statistical analysis was performed using one-way ANOVA (∗∗∗∗ p < 0.0001). Error bars represent mean ± SEM. (E) p16 immunostaining in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as antibody staining signal over imaging field. ∗∗∗∗ p < 0.0001 after unpaired t test. Error bars represent mean ± SEM. Scale bars, 50 μm. (F) Ki67 immunostaining in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as number of Ki67 positive cells normalized over μm of dermal epidermal junction (count/μm) ∗∗∗∗ p < 0.0001 after unpaired t test. Error bars represent mean ± SEM. Scale bars, 50 μm. (G) Keratin 10 immunostaining in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as fluorescence intensity over epidermis area. ∗∗∗∗ p < 0.0001 after unpaired t test. Error bars represent mean ± SEM. Scale bars, 50 μm.
    Nheks, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effect of x-PVA/HA on HaCaT Cell Viability. (a) Confocal microscopy orthogonal projection images showing dead cells (PI + , red fluorescence), total cells (PKH67, green fluorescence), and merged images (PI + /PKH67) under control, adherent, and spheroid-like conditions after 24 h of exposure to x-PVA/HA (20× objective; scale bar = 20 μm). (b) Graphical representation of the ratio between the sum of red fluorescence intensity (PI + dead cells) and the sum of green fluorescence intensity (PKH67, total cells) in control (8.54 ± 1.43) and x-PVA/HA-exposed adherent cells (7.56 ± 0.67). Data are presented as mean ± SEM. No statistically significant difference in the proportion of dead cells was observed between the two conditions (Student’s t-test: control vs. x-PVA/HA adherent cells, p > 0.05; ns, not significant). x-PVA/HA-exposed spheroid-like cells showed a low number of PI + dead cells; however, these values were not quantitatively compared with the control condition because control cells cultured on plastic did not form spheroid-like structures.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Electrospun poly(vinyl alcohol)/hyaluronic acid nanofiber mats for wound care in space: cytocompatibility and effects of UV-C treatment and simulated microgravity

    doi: 10.3389/fbioe.2026.1946755

    Figure Lengend Snippet: Effect of x-PVA/HA on HaCaT Cell Viability. (a) Confocal microscopy orthogonal projection images showing dead cells (PI + , red fluorescence), total cells (PKH67, green fluorescence), and merged images (PI + /PKH67) under control, adherent, and spheroid-like conditions after 24 h of exposure to x-PVA/HA (20× objective; scale bar = 20 μm). (b) Graphical representation of the ratio between the sum of red fluorescence intensity (PI + dead cells) and the sum of green fluorescence intensity (PKH67, total cells) in control (8.54 ± 1.43) and x-PVA/HA-exposed adherent cells (7.56 ± 0.67). Data are presented as mean ± SEM. No statistically significant difference in the proportion of dead cells was observed between the two conditions (Student’s t-test: control vs. x-PVA/HA adherent cells, p > 0.05; ns, not significant). x-PVA/HA-exposed spheroid-like cells showed a low number of PI + dead cells; however, these values were not quantitatively compared with the control condition because control cells cultured on plastic did not form spheroid-like structures.

    Article Snippet: NT2D1 human embryonal carcinoma cells, HEK293 human embryonic kidney cells, and HaCaT human keratinocytes were obtained from the American Type Culture Collection (ATCC, Manassas, VA, United States).

    Techniques: Confocal, Microscopy, Fluorescence, Control, Cell Culture

    UA beneficial impact on human keratinocytes and reconstituted human epidermis (A) Mitochondrial respiration (basal and maximal) assessed as oxygen consumption rate (OCR) in human dermal keratinocytes after 72 h of differentiation from young donor treated with DMSO for 24 h or the indicated dose of UA. ∗ p < 0.05 and ∗∗∗ p < 0.001 after one-way ANOVA. Error bars represent mean ± SEM. (B) TOMM20 immunostaining of skin epidermis in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as antibody staining signal over imaging field. ∗ p < 0.05 and ∗∗∗∗ p < 0.0001 after unpaired t test one-way ANOVA. Error bars represent mean ± SEM. Scale bars, 50 μm. (C) Relative gene expression of PINK1 and PARK2 in reconstructed human epidermis (RHE). RHE tissues were moisturized for 24 h with or without 1% Mitopure, and relative expression levels of PINK1 and PARK2 were quantified. Statistical analysis was performed using one-way ANOVA (∗ p < 0.05; ∗∗ p < 0.01). Error bars represent mean ± SEM. (D) Interleukin 6 levels normalized to cell viability in keratinocytes. Keratinocytes were stimulated with a pro-inflammatory cytokine mix for 24 h then treated for an additional 24 h with either DMSO or 1 μM UA. IL-6 secretion was quantified and normalized to cell viability. Statistical analysis was performed using one-way ANOVA (∗∗∗∗ p < 0.0001). Error bars represent mean ± SEM. (E) p16 immunostaining in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as antibody staining signal over imaging field. ∗∗∗∗ p < 0.0001 after unpaired t test. Error bars represent mean ± SEM. Scale bars, 50 μm. (F) Ki67 immunostaining in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as number of Ki67 positive cells normalized over μm of dermal epidermal junction (count/μm) ∗∗∗∗ p < 0.0001 after unpaired t test. Error bars represent mean ± SEM. Scale bars, 50 μm. (G) Keratin 10 immunostaining in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as fluorescence intensity over epidermis area. ∗∗∗∗ p < 0.0001 after unpaired t test. Error bars represent mean ± SEM. Scale bars, 50 μm.

    Journal: iScience

    Article Title: Topical application of urolithin A activates mitophagy and enhances mitochondrial function to promote skin longevity

    doi: 10.1016/j.isci.2026.117646

    Figure Lengend Snippet: UA beneficial impact on human keratinocytes and reconstituted human epidermis (A) Mitochondrial respiration (basal and maximal) assessed as oxygen consumption rate (OCR) in human dermal keratinocytes after 72 h of differentiation from young donor treated with DMSO for 24 h or the indicated dose of UA. ∗ p < 0.05 and ∗∗∗ p < 0.001 after one-way ANOVA. Error bars represent mean ± SEM. (B) TOMM20 immunostaining of skin epidermis in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as antibody staining signal over imaging field. ∗ p < 0.05 and ∗∗∗∗ p < 0.0001 after unpaired t test one-way ANOVA. Error bars represent mean ± SEM. Scale bars, 50 μm. (C) Relative gene expression of PINK1 and PARK2 in reconstructed human epidermis (RHE). RHE tissues were moisturized for 24 h with or without 1% Mitopure, and relative expression levels of PINK1 and PARK2 were quantified. Statistical analysis was performed using one-way ANOVA (∗ p < 0.05; ∗∗ p < 0.01). Error bars represent mean ± SEM. (D) Interleukin 6 levels normalized to cell viability in keratinocytes. Keratinocytes were stimulated with a pro-inflammatory cytokine mix for 24 h then treated for an additional 24 h with either DMSO or 1 μM UA. IL-6 secretion was quantified and normalized to cell viability. Statistical analysis was performed using one-way ANOVA (∗∗∗∗ p < 0.0001). Error bars represent mean ± SEM. (E) p16 immunostaining in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as antibody staining signal over imaging field. ∗∗∗∗ p < 0.0001 after unpaired t test. Error bars represent mean ± SEM. Scale bars, 50 μm. (F) Ki67 immunostaining in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as number of Ki67 positive cells normalized over μm of dermal epidermal junction (count/μm) ∗∗∗∗ p < 0.0001 after unpaired t test. Error bars represent mean ± SEM. Scale bars, 50 μm. (G) Keratin 10 immunostaining in a human full-thickness skin model from an old donor treated with or without UA 1 μM for 25 days. Data expressed as fluorescence intensity over epidermis area. ∗∗∗∗ p < 0.0001 after unpaired t test. Error bars represent mean ± SEM. Scale bars, 50 μm.

    Article Snippet: Human: Normal Human Epidermal Keratinocytes (NHEK) , PromoCell , Cat#C-12003; Lot#479Z011.1.

    Techniques: Immunostaining, Staining, Imaging, Gene Expression, Expressing, Analysis, Fluorescence

    Journal: iScience

    Article Title: Topical application of urolithin A activates mitophagy and enhances mitochondrial function to promote skin longevity

    doi: 10.1016/j.isci.2026.117646

    Figure Lengend Snippet:

    Article Snippet: Human: Normal Human Epidermal Keratinocytes (NHEK) , PromoCell , Cat#C-12003; Lot#479Z011.1.

    Techniques: Binding Assay, Ex Vivo, Recombinant, Metals, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Cell Viability Assay, Staining, DC Protein Assay, Gene Expression, Glass, Software, Imaging, Microscopy, Real-time Polymerase Chain Reaction