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culture he mouse breast cancer cell lines eo771  (ATCC)


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    ATCC culture he mouse breast cancer cell lines eo771
    Culture He Mouse Breast Cancer Cell Lines Eo771, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 268 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eo771+cells/EO771/pm42230325-292-7-21
    Average 98 stars, based on 268 article reviews
    culture he mouse breast cancer cell lines eo771 - by Bioz Stars, 2026-10
    98/100 stars

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    Multiple Displacement Amplification:

    Article Title: Blocking WNT7A Enhances MHC-I Antigen Presentation and Enhances the Effectiveness of Immune Checkpoint Blockade Therapy
    Article Snippet: .. MDA-MB-231 cells (human breast cancer cell line, RRID: CVCL_0062), SW480 cells (human colon cancer cell lines, RRID: CVCL_0546), and EO771 cells (C57BL/6 murine TNBC breast cancer cell line, RRID: CVCL_GR23) were obtained from the ATCC in 2017. .. MC38 cells (C57BL/6 murine colon cancer cell line, RRID: CVCL B288) were obtained from Kerafast in 2019.

    Article Title: Blocking WNT7A Enhances MHC-I Antigen Presentation and Enhances the Effectiveness of Immune Checkpoint Blockade Therapy
    Article Snippet: .. MDA-MB-231 cells (human breast cancer cell line, RRID: CVCL_0062), SW480 cells (human colon cancer cell lines, RRID: CVCL_0546), and EO771 cells (C57BL/6 murine TNBC breast cancer cell line, RRID: CVCL_GR23) were obtained from the ATCC in 2017. .. MC38 cells (C57BL/6 murine colon cancer cell line, RRID: CVCL B288) were obtained from Kerafast in 2019.

    Cell Culture:

    Article Title: Precancer exercise capacity and metabolism during tumor development coordinate the skeletal muscle-tumor metabolic competition.
    Article Snippet: .. YUMMER1.7 cells (47) were obtained from Marcus Bosenberg, and EO771 cells from ATCC, and cultured in standard culture media (SI Appendix). ..

    other:

    Article Title: The impact of a high fat diet and platelet activation on pre-metastatic niche formation.
    Article Snippet: EO771 cells were purchased from CH3, the Py230 cell line was purchased from the ATCC andHUVECs were obtained fromLonza.

    Article Title: The impact of a high fat diet and platelet activation on pre-metastatic niche formation
    Article Snippet: EO771 cells were purchased from CH3, the Py230 cell line was purchased from the ATCC and HUVECs were obtained from Lonza.

    Article Title: A microbiota-derived bile acid overcomes antibiotic-induced hyporesponsiveness to immune checkpoint therapy by enhancing CD8 + T cell antitumor immunity
    Article Snippet: B16-F10 cells (ATCC CRL-6475; RRID:CVC_0159), B16-OVA cells (gift from Dr. Yangxin Fu, School of Basic Medical Sciences, Tsinghua University, RRID:CVCL_WM78), A375 cells (ATCC CRL-1619, RRID:CVCL_0132) and EO771 cells (ATCC CRL-3461; RRID:CVCL_GR23), were grown at 37°C under 5% CO2 in DMEM medium (Gibco, #11995-065) supplemented with 10% heat-inactivated FBS (Corning, #MT35016CV).

    Real-time Polymerase Chain Reaction:

    Article Title: Encapsulation of doxorubicin in magnesium acetate liposomes as a pH-sensitive drug carrier for tumor therapy
    Article Snippet: .. The folate receptor α (FOLR1) gene mRNA expressions of EO771 cells and a known folate receptor-expressing mouse mammary carcinoma 4T1 cells supplied by ATCC were determined by the qPCR assay. ..

    Derivative Assay:

    Article Title: A Novel Therapeutic Approach: Notch Inhibition Enhances Radiotherapy and Checkpoint Blockade Therapy via Reprogramming of the Tumor Microenvironment
    Article Snippet: .. EO771 cells (purchased from ATCC) and the 9464D cells (derived from a TH-MYCN transgenic neuroblastoma mouse, obtained as a gift from Dr. ..

    Transgenic Assay:

    Article Title: A Novel Therapeutic Approach: Notch Inhibition Enhances Radiotherapy and Checkpoint Blockade Therapy via Reprogramming of the Tumor Microenvironment
    Article Snippet: .. EO771 cells (purchased from ATCC) and the 9464D cells (derived from a TH-MYCN transgenic neuroblastoma mouse, obtained as a gift from Dr. ..



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    culture he mouse breast cancer cell lines eo771  (ATCC)
    98
    ATCC culture he mouse breast cancer cell lines eo771
    Culture He Mouse Breast Cancer Cell Lines Eo771, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eo771+cells/EO771/pm42230325-292-7-21
    Average 98 stars, based on 1 article reviews
    culture he mouse breast cancer cell lines eo771 - by Bioz Stars, 2026-10
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    murine luminal b breast cancer cell line eo771  (ATCC)
    98
    ATCC murine luminal b breast cancer cell line eo771
    Intra-tumoral adenosine concentration (Mean ± SEM) in (A) human and (B) murine breast tumors and normal breast tissue quantified by LC-MS. (C) IHC staining: Magnification 200 X, Scale 100 μM and (D, E) H-score (Mean ± SEM) of CD73 and A 2a R in human normal breast tissue (epithelial cells, immune cells) and breast tumors (cancer cells, immune cells). (F) Relative expression of genes in the adenosine signaling axis in <t>EO771</t> and 4T1 murine breast cancer cells compared to GAPDH by qPCR. (G) Frequency of immune cell subtypes (Mean ± SEM) in EO771 tumors by spectral flow cytometry. (H) Frequency of immune cell subtypes (Mean ± SEM) in 4T1 tumors by spectral flow cytometry. (I) Abundance of adenosine signaling proteins (Mean ± SEM). in CD45 - compartment of EO771 tumors. (J) Abundance of adenosine signaling proteins (Mean ± SEM). in CD45 - compartment of 4T1 tumors. (K) Expression of adenosine signaling proteins on CD45 + immune cells (Mean ± SEM) in EO771 tumors quantified using spectral flow cytometry. (L) Expression of adenosine signaling proteins on CD45 + immune cells (Mean ± SEM) in 4T1 tumors quantified using spectral flow cytometry. Tumors were harvested from n=10-12 mice, 25 days post-implantation. (myeloid cells (CD45 + CD11b + TCRβ - ), T cells (CD45 + CD11b - TCRβ + ), B-cells (CD45 + CD11b - CD19 + ), dendritic cells (CD45 + CD11b - CD11c + CD103 + ), NK cell – natural killer cells (CD45 + CD11b - CD11c - NK1.1 + ).
    Murine Luminal B Breast Cancer Cell Line Eo771, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    murine mammary carcinoma cells  (ATCC)
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    ATCC murine mammary carcinoma cells
    Intra-tumoral adenosine concentration (Mean ± SEM) in (A) human and (B) murine breast tumors and normal breast tissue quantified by LC-MS. (C) IHC staining: Magnification 200 X, Scale 100 μM and (D, E) H-score (Mean ± SEM) of CD73 and A 2a R in human normal breast tissue (epithelial cells, immune cells) and breast tumors (cancer cells, immune cells). (F) Relative expression of genes in the adenosine signaling axis in <t>EO771</t> and 4T1 murine breast cancer cells compared to GAPDH by qPCR. (G) Frequency of immune cell subtypes (Mean ± SEM) in EO771 tumors by spectral flow cytometry. (H) Frequency of immune cell subtypes (Mean ± SEM) in 4T1 tumors by spectral flow cytometry. (I) Abundance of adenosine signaling proteins (Mean ± SEM). in CD45 - compartment of EO771 tumors. (J) Abundance of adenosine signaling proteins (Mean ± SEM). in CD45 - compartment of 4T1 tumors. (K) Expression of adenosine signaling proteins on CD45 + immune cells (Mean ± SEM) in EO771 tumors quantified using spectral flow cytometry. (L) Expression of adenosine signaling proteins on CD45 + immune cells (Mean ± SEM) in 4T1 tumors quantified using spectral flow cytometry. Tumors were harvested from n=10-12 mice, 25 days post-implantation. (myeloid cells (CD45 + CD11b + TCRβ - ), T cells (CD45 + CD11b - TCRβ + ), B-cells (CD45 + CD11b - CD19 + ), dendritic cells (CD45 + CD11b - CD11c + CD103 + ), NK cell – natural killer cells (CD45 + CD11b - CD11c - NK1.1 + ).
    Murine Mammary Carcinoma Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    eo771 triple negative mammary tumor cell line  (ATCC)
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    Intra-tumoral adenosine concentration (Mean ± SEM) in (A) human and (B) murine breast tumors and normal breast tissue quantified by LC-MS. (C) IHC staining: Magnification 200 X, Scale 100 μM and (D, E) H-score (Mean ± SEM) of CD73 and A 2a R in human normal breast tissue (epithelial cells, immune cells) and breast tumors (cancer cells, immune cells). (F) Relative expression of genes in the adenosine signaling axis in <t>EO771</t> and 4T1 murine breast cancer cells compared to GAPDH by qPCR. (G) Frequency of immune cell subtypes (Mean ± SEM) in EO771 tumors by spectral flow cytometry. (H) Frequency of immune cell subtypes (Mean ± SEM) in 4T1 tumors by spectral flow cytometry. (I) Abundance of adenosine signaling proteins (Mean ± SEM). in CD45 - compartment of EO771 tumors. (J) Abundance of adenosine signaling proteins (Mean ± SEM). in CD45 - compartment of 4T1 tumors. (K) Expression of adenosine signaling proteins on CD45 + immune cells (Mean ± SEM) in EO771 tumors quantified using spectral flow cytometry. (L) Expression of adenosine signaling proteins on CD45 + immune cells (Mean ± SEM) in 4T1 tumors quantified using spectral flow cytometry. Tumors were harvested from n=10-12 mice, 25 days post-implantation. (myeloid cells (CD45 + CD11b + TCRβ - ), T cells (CD45 + CD11b - TCRβ + ), B-cells (CD45 + CD11b - CD19 + ), dendritic cells (CD45 + CD11b - CD11c + CD103 + ), NK cell – natural killer cells (CD45 + CD11b - CD11c - NK1.1 + ).
    Eo771 Triple Negative Mammary Tumor Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    eo771 cells  (ATCC)
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    Intra-tumoral adenosine concentration (Mean ± SEM) in (A) human and (B) murine breast tumors and normal breast tissue quantified by LC-MS. (C) IHC staining: Magnification 200 X, Scale 100 μM and (D, E) H-score (Mean ± SEM) of CD73 and A 2a R in human normal breast tissue (epithelial cells, immune cells) and breast tumors (cancer cells, immune cells). (F) Relative expression of genes in the adenosine signaling axis in <t>EO771</t> and 4T1 murine breast cancer cells compared to GAPDH by qPCR. (G) Frequency of immune cell subtypes (Mean ± SEM) in EO771 tumors by spectral flow cytometry. (H) Frequency of immune cell subtypes (Mean ± SEM) in 4T1 tumors by spectral flow cytometry. (I) Abundance of adenosine signaling proteins (Mean ± SEM). in CD45 - compartment of EO771 tumors. (J) Abundance of adenosine signaling proteins (Mean ± SEM). in CD45 - compartment of 4T1 tumors. (K) Expression of adenosine signaling proteins on CD45 + immune cells (Mean ± SEM) in EO771 tumors quantified using spectral flow cytometry. (L) Expression of adenosine signaling proteins on CD45 + immune cells (Mean ± SEM) in 4T1 tumors quantified using spectral flow cytometry. Tumors were harvested from n=10-12 mice, 25 days post-implantation. (myeloid cells (CD45 + CD11b + TCRβ - ), T cells (CD45 + CD11b - TCRβ + ), B-cells (CD45 + CD11b - CD19 + ), dendritic cells (CD45 + CD11b - CD11c + CD103 + ), NK cell – natural killer cells (CD45 + CD11b - CD11c - NK1.1 + ).
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    Intra-tumoral adenosine concentration (Mean ± SEM) in (A) human and (B) murine breast tumors and normal breast tissue quantified by LC-MS. (C) IHC staining: Magnification 200 X, Scale 100 μM and (D, E) H-score (Mean ± SEM) of CD73 and A 2a R in human normal breast tissue (epithelial cells, immune cells) and breast tumors (cancer cells, immune cells). (F) Relative expression of genes in the adenosine signaling axis in <t>EO771</t> and 4T1 murine breast cancer cells compared to GAPDH by qPCR. (G) Frequency of immune cell subtypes (Mean ± SEM) in EO771 tumors by spectral flow cytometry. (H) Frequency of immune cell subtypes (Mean ± SEM) in 4T1 tumors by spectral flow cytometry. (I) Abundance of adenosine signaling proteins (Mean ± SEM). in CD45 - compartment of EO771 tumors. (J) Abundance of adenosine signaling proteins (Mean ± SEM). in CD45 - compartment of 4T1 tumors. (K) Expression of adenosine signaling proteins on CD45 + immune cells (Mean ± SEM) in EO771 tumors quantified using spectral flow cytometry. (L) Expression of adenosine signaling proteins on CD45 + immune cells (Mean ± SEM) in 4T1 tumors quantified using spectral flow cytometry. Tumors were harvested from n=10-12 mice, 25 days post-implantation. (myeloid cells (CD45 + CD11b + TCRβ - ), T cells (CD45 + CD11b - TCRβ + ), B-cells (CD45 + CD11b - CD19 + ), dendritic cells (CD45 + CD11b - CD11c + CD103 + ), NK cell – natural killer cells (CD45 + CD11b - CD11c - NK1.1 + ).
    E0771 Lmb Gp Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    e0771 lmb cells  (ATCC)
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    ATCC e0771 lmb cells
    Intra-tumoral adenosine concentration (Mean ± SEM) in (A) human and (B) murine breast tumors and normal breast tissue quantified by LC-MS. (C) IHC staining: Magnification 200 X, Scale 100 μM and (D, E) H-score (Mean ± SEM) of CD73 and A 2a R in human normal breast tissue (epithelial cells, immune cells) and breast tumors (cancer cells, immune cells). (F) Relative expression of genes in the adenosine signaling axis in <t>EO771</t> and 4T1 murine breast cancer cells compared to GAPDH by qPCR. (G) Frequency of immune cell subtypes (Mean ± SEM) in EO771 tumors by spectral flow cytometry. (H) Frequency of immune cell subtypes (Mean ± SEM) in 4T1 tumors by spectral flow cytometry. (I) Abundance of adenosine signaling proteins (Mean ± SEM). in CD45 - compartment of EO771 tumors. (J) Abundance of adenosine signaling proteins (Mean ± SEM). in CD45 - compartment of 4T1 tumors. (K) Expression of adenosine signaling proteins on CD45 + immune cells (Mean ± SEM) in EO771 tumors quantified using spectral flow cytometry. (L) Expression of adenosine signaling proteins on CD45 + immune cells (Mean ± SEM) in 4T1 tumors quantified using spectral flow cytometry. Tumors were harvested from n=10-12 mice, 25 days post-implantation. (myeloid cells (CD45 + CD11b + TCRβ - ), T cells (CD45 + CD11b - TCRβ + ), B-cells (CD45 + CD11b - CD19 + ), dendritic cells (CD45 + CD11b - CD11c + CD103 + ), NK cell – natural killer cells (CD45 + CD11b - CD11c - NK1.1 + ).
    E0771 Lmb Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    murine eo771 breast carcinoma cell line  (ATCC)
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    ATCC murine eo771 breast carcinoma cell line
    Intra-tumoral adenosine concentration (Mean ± SEM) in (A) human and (B) murine breast tumors and normal breast tissue quantified by LC-MS. (C) IHC staining: Magnification 200 X, Scale 100 μM and (D, E) H-score (Mean ± SEM) of CD73 and A 2a R in human normal breast tissue (epithelial cells, immune cells) and breast tumors (cancer cells, immune cells). (F) Relative expression of genes in the adenosine signaling axis in <t>EO771</t> and 4T1 murine breast cancer cells compared to GAPDH by qPCR. (G) Frequency of immune cell subtypes (Mean ± SEM) in EO771 tumors by spectral flow cytometry. (H) Frequency of immune cell subtypes (Mean ± SEM) in 4T1 tumors by spectral flow cytometry. (I) Abundance of adenosine signaling proteins (Mean ± SEM). in CD45 - compartment of EO771 tumors. (J) Abundance of adenosine signaling proteins (Mean ± SEM). in CD45 - compartment of 4T1 tumors. (K) Expression of adenosine signaling proteins on CD45 + immune cells (Mean ± SEM) in EO771 tumors quantified using spectral flow cytometry. (L) Expression of adenosine signaling proteins on CD45 + immune cells (Mean ± SEM) in 4T1 tumors quantified using spectral flow cytometry. Tumors were harvested from n=10-12 mice, 25 days post-implantation. (myeloid cells (CD45 + CD11b + TCRβ - ), T cells (CD45 + CD11b - TCRβ + ), B-cells (CD45 + CD11b - CD19 + ), dendritic cells (CD45 + CD11b - CD11c + CD103 + ), NK cell – natural killer cells (CD45 + CD11b - CD11c - NK1.1 + ).
    Murine Eo771 Breast Carcinoma Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    eo771 cells  (Jackson Laboratory)
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    Jackson Laboratory eo771 cells
    Intra-tumoral adenosine concentration (Mean ± SEM) in (A) human and (B) murine breast tumors and normal breast tissue quantified by LC-MS. (C) IHC staining: Magnification 200 X, Scale 100 μM and (D, E) H-score (Mean ± SEM) of CD73 and A 2a R in human normal breast tissue (epithelial cells, immune cells) and breast tumors (cancer cells, immune cells). (F) Relative expression of genes in the adenosine signaling axis in <t>EO771</t> and 4T1 murine breast cancer cells compared to GAPDH by qPCR. (G) Frequency of immune cell subtypes (Mean ± SEM) in EO771 tumors by spectral flow cytometry. (H) Frequency of immune cell subtypes (Mean ± SEM) in 4T1 tumors by spectral flow cytometry. (I) Abundance of adenosine signaling proteins (Mean ± SEM). in CD45 - compartment of EO771 tumors. (J) Abundance of adenosine signaling proteins (Mean ± SEM). in CD45 - compartment of 4T1 tumors. (K) Expression of adenosine signaling proteins on CD45 + immune cells (Mean ± SEM) in EO771 tumors quantified using spectral flow cytometry. (L) Expression of adenosine signaling proteins on CD45 + immune cells (Mean ± SEM) in 4T1 tumors quantified using spectral flow cytometry. Tumors were harvested from n=10-12 mice, 25 days post-implantation. (myeloid cells (CD45 + CD11b + TCRβ - ), T cells (CD45 + CD11b - TCRβ + ), B-cells (CD45 + CD11b - CD19 + ), dendritic cells (CD45 + CD11b - CD11c + CD103 + ), NK cell – natural killer cells (CD45 + CD11b - CD11c - NK1.1 + ).
    Eo771 Cells, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    e0771 crl 3461 cells  (ATCC)
    98
    ATCC e0771 crl 3461 cells
    Intra-tumoral adenosine concentration (Mean ± SEM) in (A) human and (B) murine breast tumors and normal breast tissue quantified by LC-MS. (C) IHC staining: Magnification 200 X, Scale 100 μM and (D, E) H-score (Mean ± SEM) of CD73 and A 2a R in human normal breast tissue (epithelial cells, immune cells) and breast tumors (cancer cells, immune cells). (F) Relative expression of genes in the adenosine signaling axis in <t>EO771</t> and 4T1 murine breast cancer cells compared to GAPDH by qPCR. (G) Frequency of immune cell subtypes (Mean ± SEM) in EO771 tumors by spectral flow cytometry. (H) Frequency of immune cell subtypes (Mean ± SEM) in 4T1 tumors by spectral flow cytometry. (I) Abundance of adenosine signaling proteins (Mean ± SEM). in CD45 - compartment of EO771 tumors. (J) Abundance of adenosine signaling proteins (Mean ± SEM). in CD45 - compartment of 4T1 tumors. (K) Expression of adenosine signaling proteins on CD45 + immune cells (Mean ± SEM) in EO771 tumors quantified using spectral flow cytometry. (L) Expression of adenosine signaling proteins on CD45 + immune cells (Mean ± SEM) in 4T1 tumors quantified using spectral flow cytometry. Tumors were harvested from n=10-12 mice, 25 days post-implantation. (myeloid cells (CD45 + CD11b + TCRβ - ), T cells (CD45 + CD11b - TCRβ + ), B-cells (CD45 + CD11b - CD19 + ), dendritic cells (CD45 + CD11b - CD11c + CD103 + ), NK cell – natural killer cells (CD45 + CD11b - CD11c - NK1.1 + ).
    E0771 Crl 3461 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Intra-tumoral adenosine concentration (Mean ± SEM) in (A) human and (B) murine breast tumors and normal breast tissue quantified by LC-MS. (C) IHC staining: Magnification 200 X, Scale 100 μM and (D, E) H-score (Mean ± SEM) of CD73 and A 2a R in human normal breast tissue (epithelial cells, immune cells) and breast tumors (cancer cells, immune cells). (F) Relative expression of genes in the adenosine signaling axis in EO771 and 4T1 murine breast cancer cells compared to GAPDH by qPCR. (G) Frequency of immune cell subtypes (Mean ± SEM) in EO771 tumors by spectral flow cytometry. (H) Frequency of immune cell subtypes (Mean ± SEM) in 4T1 tumors by spectral flow cytometry. (I) Abundance of adenosine signaling proteins (Mean ± SEM). in CD45 - compartment of EO771 tumors. (J) Abundance of adenosine signaling proteins (Mean ± SEM). in CD45 - compartment of 4T1 tumors. (K) Expression of adenosine signaling proteins on CD45 + immune cells (Mean ± SEM) in EO771 tumors quantified using spectral flow cytometry. (L) Expression of adenosine signaling proteins on CD45 + immune cells (Mean ± SEM) in 4T1 tumors quantified using spectral flow cytometry. Tumors were harvested from n=10-12 mice, 25 days post-implantation. (myeloid cells (CD45 + CD11b + TCRβ - ), T cells (CD45 + CD11b - TCRβ + ), B-cells (CD45 + CD11b - CD19 + ), dendritic cells (CD45 + CD11b - CD11c + CD103 + ), NK cell – natural killer cells (CD45 + CD11b - CD11c - NK1.1 + ).

    Journal: bioRxiv

    Article Title: Targeting CD73-A 2a R-Mediated Adenosine Signaling at the Tumor-Immune Interface Overcomes Radioresistance

    doi: 10.64898/2026.05.26.727904

    Figure Lengend Snippet: Intra-tumoral adenosine concentration (Mean ± SEM) in (A) human and (B) murine breast tumors and normal breast tissue quantified by LC-MS. (C) IHC staining: Magnification 200 X, Scale 100 μM and (D, E) H-score (Mean ± SEM) of CD73 and A 2a R in human normal breast tissue (epithelial cells, immune cells) and breast tumors (cancer cells, immune cells). (F) Relative expression of genes in the adenosine signaling axis in EO771 and 4T1 murine breast cancer cells compared to GAPDH by qPCR. (G) Frequency of immune cell subtypes (Mean ± SEM) in EO771 tumors by spectral flow cytometry. (H) Frequency of immune cell subtypes (Mean ± SEM) in 4T1 tumors by spectral flow cytometry. (I) Abundance of adenosine signaling proteins (Mean ± SEM). in CD45 - compartment of EO771 tumors. (J) Abundance of adenosine signaling proteins (Mean ± SEM). in CD45 - compartment of 4T1 tumors. (K) Expression of adenosine signaling proteins on CD45 + immune cells (Mean ± SEM) in EO771 tumors quantified using spectral flow cytometry. (L) Expression of adenosine signaling proteins on CD45 + immune cells (Mean ± SEM) in 4T1 tumors quantified using spectral flow cytometry. Tumors were harvested from n=10-12 mice, 25 days post-implantation. (myeloid cells (CD45 + CD11b + TCRβ - ), T cells (CD45 + CD11b - TCRβ + ), B-cells (CD45 + CD11b - CD19 + ), dendritic cells (CD45 + CD11b - CD11c + CD103 + ), NK cell – natural killer cells (CD45 + CD11b - CD11c - NK1.1 + ).

    Article Snippet: Murine luminal B breast cancer cell line EO771 (ERα-, ERβ+, PR+, HER2+) and triple negative breast cancer cell line 4T1 (ER-, PR-, HER2-) was procured from ATCC.

    Techniques: Concentration Assay, Liquid Chromatography with Mass Spectroscopy, Immunohistochemistry, Expressing, Flow Cytometry

    (A) Fold change in intra-tumoral adenosine concentrations in irradiated (8Gy x 1) EO771 tumors 12-, 24-, 48-, and 72-hours after treatment calculated with respect to adenosine concentration in unirradiated tumors. Abundance (Mean ± SEM) of (B) myeloid cells (CD45 + CD11b + ) and (C) T lymphocytes (CD45 + TCRb + ) in EO771 tumors on days 1,3,5,11 after irradiation (8Gy x 3) compared to unirradiated controls. Data are representative of two separate experiments, n=10-12 mice/group. (***) p<0.001, (****) p<0.0005. Log2 fold changes in gene expression in irradiated cells versus the corresponding unirradiated cells on day 1 (D1), 3 (D3), 5 (D5) in (D) CD45 + CD11b + cells (E) CD45 + TCRb + cells (F) CD45 - cells sorted from intra-tumoral cell suspensions (n=3 mice/group). Log2 fold changes in gene expression of adenosine signaling genes in irradiated cells versus the corresponding unirradiated cells on day 1, 3, 5 in (G) CD45 + CD11b + cells (H) CD45 + TCRb + cells (I) CD45 - cells sorted from intra-tumoral cell suspensions (n=3 mice/group).

    Journal: bioRxiv

    Article Title: Targeting CD73-A 2a R-Mediated Adenosine Signaling at the Tumor-Immune Interface Overcomes Radioresistance

    doi: 10.64898/2026.05.26.727904

    Figure Lengend Snippet: (A) Fold change in intra-tumoral adenosine concentrations in irradiated (8Gy x 1) EO771 tumors 12-, 24-, 48-, and 72-hours after treatment calculated with respect to adenosine concentration in unirradiated tumors. Abundance (Mean ± SEM) of (B) myeloid cells (CD45 + CD11b + ) and (C) T lymphocytes (CD45 + TCRb + ) in EO771 tumors on days 1,3,5,11 after irradiation (8Gy x 3) compared to unirradiated controls. Data are representative of two separate experiments, n=10-12 mice/group. (***) p<0.001, (****) p<0.0005. Log2 fold changes in gene expression in irradiated cells versus the corresponding unirradiated cells on day 1 (D1), 3 (D3), 5 (D5) in (D) CD45 + CD11b + cells (E) CD45 + TCRb + cells (F) CD45 - cells sorted from intra-tumoral cell suspensions (n=3 mice/group). Log2 fold changes in gene expression of adenosine signaling genes in irradiated cells versus the corresponding unirradiated cells on day 1, 3, 5 in (G) CD45 + CD11b + cells (H) CD45 + TCRb + cells (I) CD45 - cells sorted from intra-tumoral cell suspensions (n=3 mice/group).

    Article Snippet: Murine luminal B breast cancer cell line EO771 (ERα-, ERβ+, PR+, HER2+) and triple negative breast cancer cell line 4T1 (ER-, PR-, HER2-) was procured from ATCC.

    Techniques: Irradiation, Concentration Assay, Gene Expression

    (A) Adenosine concentration (Mean ± SEM) measured in cell-free supernatant from WT (EO771.lmb), ENPP1 -/- , CD73 -/- , ENPP1 -/- CD73 -/- cells grown in vitro, 18 hours after irradiation (8 Gy x 1), compared to untreated controls. (B) Tumor volume (Mean ± SEM) from WT mice bearing WT EO771, CD73 -/- EO771, or Enpp1 -/- EO771 tumors. (C, D) Tumor volume (Mean ± SEM) and survival of WT mice bearing WT EO771, CD73 -/- EO771, or Enpp1 -/- EO771 tumors irradiated (8Gy x 3) on days 13, 14, 15 post-implantation. (E, F) Tumor volume (Mean ± SEM) of orthotopic EO771 tumors implanted in wildtype (WT, C57BL6/J), CD73 -/- and A 2a R -/- A 2b R -/- mice. (G) Intra-tumoral adenosine concentration (Mean ± SEM) in paired normal murine breast tissue and EO771 tumors implanted in WT, CD73 -/- and A 2a R -/- A 2b R -/- mice. (H) Tumor volumes (Mean ± SEM) from WT (C57BL6/J), CD73 -/- , A 2a R -/- A 2b R -/- mice implanted with EO771 cells irradiated (8 Gy x 3) on days 13, 14, 15 post-implantation. Tumor volumes (Mean ± SEM) from (I) untreated and (J) radiation-treated (8Gy x 3) tumors from LysM Cre , A 2a R fl/fl , LysM Cre A 2a R fl/- , LysM Cre A 2a R fl/fl mice implanted with EO771 cells. Data are representative of two separate experiments, n=10-12 mice/group (*) p<0.05 (**), p<0.01(***), p<0.001, (****)p<0.0005.

    Journal: bioRxiv

    Article Title: Targeting CD73-A 2a R-Mediated Adenosine Signaling at the Tumor-Immune Interface Overcomes Radioresistance

    doi: 10.64898/2026.05.26.727904

    Figure Lengend Snippet: (A) Adenosine concentration (Mean ± SEM) measured in cell-free supernatant from WT (EO771.lmb), ENPP1 -/- , CD73 -/- , ENPP1 -/- CD73 -/- cells grown in vitro, 18 hours after irradiation (8 Gy x 1), compared to untreated controls. (B) Tumor volume (Mean ± SEM) from WT mice bearing WT EO771, CD73 -/- EO771, or Enpp1 -/- EO771 tumors. (C, D) Tumor volume (Mean ± SEM) and survival of WT mice bearing WT EO771, CD73 -/- EO771, or Enpp1 -/- EO771 tumors irradiated (8Gy x 3) on days 13, 14, 15 post-implantation. (E, F) Tumor volume (Mean ± SEM) of orthotopic EO771 tumors implanted in wildtype (WT, C57BL6/J), CD73 -/- and A 2a R -/- A 2b R -/- mice. (G) Intra-tumoral adenosine concentration (Mean ± SEM) in paired normal murine breast tissue and EO771 tumors implanted in WT, CD73 -/- and A 2a R -/- A 2b R -/- mice. (H) Tumor volumes (Mean ± SEM) from WT (C57BL6/J), CD73 -/- , A 2a R -/- A 2b R -/- mice implanted with EO771 cells irradiated (8 Gy x 3) on days 13, 14, 15 post-implantation. Tumor volumes (Mean ± SEM) from (I) untreated and (J) radiation-treated (8Gy x 3) tumors from LysM Cre , A 2a R fl/fl , LysM Cre A 2a R fl/- , LysM Cre A 2a R fl/fl mice implanted with EO771 cells. Data are representative of two separate experiments, n=10-12 mice/group (*) p<0.05 (**), p<0.01(***), p<0.001, (****)p<0.0005.

    Article Snippet: Murine luminal B breast cancer cell line EO771 (ERα-, ERβ+, PR+, HER2+) and triple negative breast cancer cell line 4T1 (ER-, PR-, HER2-) was procured from ATCC.

    Techniques: Concentration Assay, In Vitro, Irradiation

    (A) Schematic summary of treatment schedule for EO771 or 4T1 orthotopic breast cancer model. (B) Tumor volume measurements (Mean ± SEM) beginning 8 days after EO771 implantation (n=8-10 mice/group). AB680 (quemliclustat, CD73 inhibitor), AB928 (etrumadenant, A 2a R/A 2b R antagonist), αPD1 (zimberelimab, anti-PD-1 antibody), isotype control (IgG2 antibody), tumor irradiation (RT, 8 Gy x 3; 12-, 13-, 14- days after implantation). (C) Tumor volumes from days 8-42 of irradiated mice treated in combination with isotype control, anti-PD-1, AB928 and AB680, AB928 and AB680 and anti-PD-1. (D) Spider plots of tumors from mice belonging to all treatment groups implanted with EO771 cells. (E) Slopes (Mean ± SEM) during tumor rebound period (days 32-42) from growth curves of mice belonging to irradiation and anti-PD-1, irradiation and AB680 and AB928, irradiation and AB680 and AB928 and anti-PD-1 treatment groups. (F) Tumor volume measurements (Mean ± SEM) beginning 8 days after 4T1 implantation. Data are representative of two separate experiments, n=8-10 mice/group (*) p<0.05 (**), p<0.01, (****) p<0.0005.

    Journal: bioRxiv

    Article Title: Targeting CD73-A 2a R-Mediated Adenosine Signaling at the Tumor-Immune Interface Overcomes Radioresistance

    doi: 10.64898/2026.05.26.727904

    Figure Lengend Snippet: (A) Schematic summary of treatment schedule for EO771 or 4T1 orthotopic breast cancer model. (B) Tumor volume measurements (Mean ± SEM) beginning 8 days after EO771 implantation (n=8-10 mice/group). AB680 (quemliclustat, CD73 inhibitor), AB928 (etrumadenant, A 2a R/A 2b R antagonist), αPD1 (zimberelimab, anti-PD-1 antibody), isotype control (IgG2 antibody), tumor irradiation (RT, 8 Gy x 3; 12-, 13-, 14- days after implantation). (C) Tumor volumes from days 8-42 of irradiated mice treated in combination with isotype control, anti-PD-1, AB928 and AB680, AB928 and AB680 and anti-PD-1. (D) Spider plots of tumors from mice belonging to all treatment groups implanted with EO771 cells. (E) Slopes (Mean ± SEM) during tumor rebound period (days 32-42) from growth curves of mice belonging to irradiation and anti-PD-1, irradiation and AB680 and AB928, irradiation and AB680 and AB928 and anti-PD-1 treatment groups. (F) Tumor volume measurements (Mean ± SEM) beginning 8 days after 4T1 implantation. Data are representative of two separate experiments, n=8-10 mice/group (*) p<0.05 (**), p<0.01, (****) p<0.0005.

    Article Snippet: Murine luminal B breast cancer cell line EO771 (ERα-, ERβ+, PR+, HER2+) and triple negative breast cancer cell line 4T1 (ER-, PR-, HER2-) was procured from ATCC.

    Techniques: Control, Irradiation