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Merck KGaA slanetz-bartley enterococcus selective agar
Slanetz Bartley Enterococcus Selective Agar, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/enterococcus+selective+agar/enterococcus+selective+agar/pm40298543-110-16-20
Average 90 stars, based on 1 article reviews
slanetz-bartley enterococcus selective agar - by Bioz Stars, 2026-09
90/100 stars

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Incubation:

Article Title: Microbial diversity, morpho-textural characterization, and volatilome profile of the Portuguese thistle-curdled cheese Queijo da Beira Baixa PDO.
Article Snippet: The aim of the present study was to characterize the bacterial and fungal communities naturally occurring in Queijo da Beira Baixa PDO cheese samples produced in Castelo Branco district (Beira Baixa Region, Portugal) through viable counts and metataxonomic analyses.. Physico-chemical and morpho-textural analyses were also carried out, together with the analysis of volatile organic compounds (VOCs).. In the analyzed samples, pH values ranged between 4.72 ± 0.15 and 5.85 ± 0.02, with values of lactic acid content comprised between 0.64 ± 0.00 and 1.95 ± 0.16 g 100 g 1.

Article Title: Tasting of traditional Polish fermented cucumbers: Microbiology, morpho-textural features, and volatilome.
Article Snippet: For cucumbers, viable counts were performed by mixing 10 g of each sample with 90 mL of sterile peptone water (1 g L-1 of bacteriological peptone) homogenized using a Stomacher apparatus (400 Circulator, International PBI, Milan, Italy) for 3 min at 260 rpm. .. After cucumbers homogenization, and also for brine samples, ten-fold serial dilutions were prepared and viable counts of the following microbial groups were evaluated: i) presumptive lactobacilli on De Man Rogosa and Sharpe (MRS) agar (VWR Prolabo Chemicals, Leuven, Belgium) medium supplemented with cycloheximide (250 mg L-1) incubated at 37 ◦C for 48–72 h; ii) presumptive lactococci on M17 agar (VWR) medium supplemented with cycloheximide (250 mg L-1) incubated at 22 ◦C for 48–72 h; iii) presumptive thermophilic cocci on M17 (VWR) medium supplemented with cycloheximide (250 mg L-1) incubated at 42 ◦C for 48–72 h; iv) coagulase-negative cocci on Mannitol Salt Agar (MSA) (Liofilchem, Roseto degli Abruzzi, Italy) incubated at 37 ◦C for 48–72 h; v) enterococci on Enterococcus Selective Agar (Merck KGAa, Darmstadt, Germany) incubated at 37 ◦C for 48 h; vi) Enterobacteriaceae on Violet Red Bile Glucose Agar (VRBGA) (VWR) incubated at 37 ◦C for 24 h; vii) eumycetes on Rose Bengal Chloramphenicol Agar (VWR) incubated at 25 ◦C for 72–96 h. The results of two biological and three technical replicates were expressed as the log of colony forming units (cfu) per gram or mL of sample and reported as mean ± standard deviation. ..

Article Title: Microbiological, morpho-textural, and volatile characterization of Portuguese Queijo de Nisa PDO cheese.
Article Snippet: Queijo de Nisa PDO (Protected Designation of Origin) is a semi-hard cheese obtained from raw ewe’s milk clotted with cardoon (Cynara cardunculus, L.) rennet.. The aim of the present study was to characterize the bacterial communities naturally occurring in Queijo de Nisa PDO cheese samples through viable counting and metataxonomic analysis.. Moreover, physico-chemical and morpho-textural analyses were also performed, together with the analysis of volatile organic compounds (VOCs) through solid-phase microextraction (SPME) coupled with gas chromatography-mass spectrometry (GC–MS).

Standard Deviation:

Article Title: Microbial diversity, morpho-textural characterization, and volatilome profile of the Portuguese thistle-curdled cheese Queijo da Beira Baixa PDO.
Article Snippet: The aim of the present study was to characterize the bacterial and fungal communities naturally occurring in Queijo da Beira Baixa PDO cheese samples produced in Castelo Branco district (Beira Baixa Region, Portugal) through viable counts and metataxonomic analyses.. Physico-chemical and morpho-textural analyses were also carried out, together with the analysis of volatile organic compounds (VOCs).. In the analyzed samples, pH values ranged between 4.72 ± 0.15 and 5.85 ± 0.02, with values of lactic acid content comprised between 0.64 ± 0.00 and 1.95 ± 0.16 g 100 g 1.

Article Title: Tasting of traditional Polish fermented cucumbers: Microbiology, morpho-textural features, and volatilome.
Article Snippet: For cucumbers, viable counts were performed by mixing 10 g of each sample with 90 mL of sterile peptone water (1 g L-1 of bacteriological peptone) homogenized using a Stomacher apparatus (400 Circulator, International PBI, Milan, Italy) for 3 min at 260 rpm. .. After cucumbers homogenization, and also for brine samples, ten-fold serial dilutions were prepared and viable counts of the following microbial groups were evaluated: i) presumptive lactobacilli on De Man Rogosa and Sharpe (MRS) agar (VWR Prolabo Chemicals, Leuven, Belgium) medium supplemented with cycloheximide (250 mg L-1) incubated at 37 ◦C for 48–72 h; ii) presumptive lactococci on M17 agar (VWR) medium supplemented with cycloheximide (250 mg L-1) incubated at 22 ◦C for 48–72 h; iii) presumptive thermophilic cocci on M17 (VWR) medium supplemented with cycloheximide (250 mg L-1) incubated at 42 ◦C for 48–72 h; iv) coagulase-negative cocci on Mannitol Salt Agar (MSA) (Liofilchem, Roseto degli Abruzzi, Italy) incubated at 37 ◦C for 48–72 h; v) enterococci on Enterococcus Selective Agar (Merck KGAa, Darmstadt, Germany) incubated at 37 ◦C for 48 h; vi) Enterobacteriaceae on Violet Red Bile Glucose Agar (VRBGA) (VWR) incubated at 37 ◦C for 24 h; vii) eumycetes on Rose Bengal Chloramphenicol Agar (VWR) incubated at 25 ◦C for 72–96 h. The results of two biological and three technical replicates were expressed as the log of colony forming units (cfu) per gram or mL of sample and reported as mean ± standard deviation. ..

Article Title: Microbiological, morpho-textural, and volatile characterization of Portuguese Queijo de Nisa PDO cheese.
Article Snippet: Queijo de Nisa PDO (Protected Designation of Origin) is a semi-hard cheese obtained from raw ewe’s milk clotted with cardoon (Cynara cardunculus, L.) rennet.. The aim of the present study was to characterize the bacterial communities naturally occurring in Queijo de Nisa PDO cheese samples through viable counting and metataxonomic analysis.. Moreover, physico-chemical and morpho-textural analyses were also performed, together with the analysis of volatile organic compounds (VOCs) through solid-phase microextraction (SPME) coupled with gas chromatography-mass spectrometry (GC–MS).

Homogenization:

Article Title: Tasting of traditional Polish fermented cucumbers: Microbiology, morpho-textural features, and volatilome.
Article Snippet: For cucumbers, viable counts were performed by mixing 10 g of each sample with 90 mL of sterile peptone water (1 g L-1 of bacteriological peptone) homogenized using a Stomacher apparatus (400 Circulator, International PBI, Milan, Italy) for 3 min at 260 rpm. .. After cucumbers homogenization, and also for brine samples, ten-fold serial dilutions were prepared and viable counts of the following microbial groups were evaluated: i) presumptive lactobacilli on De Man Rogosa and Sharpe (MRS) agar (VWR Prolabo Chemicals, Leuven, Belgium) medium supplemented with cycloheximide (250 mg L-1) incubated at 37 ◦C for 48–72 h; ii) presumptive lactococci on M17 agar (VWR) medium supplemented with cycloheximide (250 mg L-1) incubated at 22 ◦C for 48–72 h; iii) presumptive thermophilic cocci on M17 (VWR) medium supplemented with cycloheximide (250 mg L-1) incubated at 42 ◦C for 48–72 h; iv) coagulase-negative cocci on Mannitol Salt Agar (MSA) (Liofilchem, Roseto degli Abruzzi, Italy) incubated at 37 ◦C for 48–72 h; v) enterococci on Enterococcus Selective Agar (Merck KGAa, Darmstadt, Germany) incubated at 37 ◦C for 48 h; vi) Enterobacteriaceae on Violet Red Bile Glucose Agar (VRBGA) (VWR) incubated at 37 ◦C for 24 h; vii) eumycetes on Rose Bengal Chloramphenicol Agar (VWR) incubated at 25 ◦C for 72–96 h. The results of two biological and three technical replicates were expressed as the log of colony forming units (cfu) per gram or mL of sample and reported as mean ± standard deviation. ..



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Image Search Results


Identification and molecular characterization of E. faecium isolates. ( A ) Colony morphology on Enterococcus-selective agar showing brown-black colonies with brown halos. ( B ) Colony morphology on blood agar with small, round, slightly raised, grayish-white, translucent colonies. ( C ) Gram staining showing Gram-positive cocci, occurring singly or in pairs. ( D ) PCR amplification of the ddl gene showing a 176 bp product specific to E. faecium ; Lanes 1–3 in correspond to isolates SCQ3, SCQ4, and SCQ11, respectively. ( E ) 16S rRNA gene amplification producing an approximately 1500 bp band; Lanes 1–3 in correspond to isolates to SCQ3, SCQ4, and SCQ11, respectively. ( F ) Neighbor-joining phylogenetic tree constructed using MEGA X from aligned 16S rRNA sequences, illustrating genetic relationships between isolates and reference E. faecium strains from NCBI. Bootstrap values (n = 1000 replicates) shown at branches; scale bar indicates evolutionary distance.

Journal: Veterinary Sciences

Article Title: Isolation and Characterization of Vancomycin-Resistant Enterococcus faecium from Cattle: Antimicrobial Resistance, Virulence Genes, and Pathogenicity

doi: 10.3390/vetsci12090880

Figure Lengend Snippet: Identification and molecular characterization of E. faecium isolates. ( A ) Colony morphology on Enterococcus-selective agar showing brown-black colonies with brown halos. ( B ) Colony morphology on blood agar with small, round, slightly raised, grayish-white, translucent colonies. ( C ) Gram staining showing Gram-positive cocci, occurring singly or in pairs. ( D ) PCR amplification of the ddl gene showing a 176 bp product specific to E. faecium ; Lanes 1–3 in correspond to isolates SCQ3, SCQ4, and SCQ11, respectively. ( E ) 16S rRNA gene amplification producing an approximately 1500 bp band; Lanes 1–3 in correspond to isolates to SCQ3, SCQ4, and SCQ11, respectively. ( F ) Neighbor-joining phylogenetic tree constructed using MEGA X from aligned 16S rRNA sequences, illustrating genetic relationships between isolates and reference E. faecium strains from NCBI. Bootstrap values (n = 1000 replicates) shown at branches; scale bar indicates evolutionary distance.

Article Snippet: Cultures were then streaked onto Pfizer enterococcus selective agar plates (Pfizer Inc., Shanghai, China) and incubated at 37 °C for 18–24 h. Colonies appeared as brown/black with brown halos, and the colony morphology was observed and recorded.

Techniques: Staining, Amplification, Construct

Antibiofilm activity of Enterococcus phages . (a) Minimum biofilm eradication concentration presented as percent reduction in the biofilm biomass compared to non-treated control using phage titres ranging from (9-5) log 10 PFU/mL. (b) antibiofilm activity of Enterococcus phages against host ATCC-700802 and clinical isolates 8010 and 8050. Data presented as mean ± SD n=3. ns: non-significant, p > 0.05, *p < 0.05; **p < 0.01, ***p < 0.001 one-way ANOVA followed by multiple comparisons tests.

Journal: bioRxiv

Article Title: LicD-Mediated Cell Wall Decoration Governs Phage Sensitivity in Enterococcus faecalis Clinical Isolates

doi: 10.1101/2025.05.13.653012

Figure Lengend Snippet: Antibiofilm activity of Enterococcus phages . (a) Minimum biofilm eradication concentration presented as percent reduction in the biofilm biomass compared to non-treated control using phage titres ranging from (9-5) log 10 PFU/mL. (b) antibiofilm activity of Enterococcus phages against host ATCC-700802 and clinical isolates 8010 and 8050. Data presented as mean ± SD n=3. ns: non-significant, p > 0.05, *p < 0.05; **p < 0.01, ***p < 0.001 one-way ANOVA followed by multiple comparisons tests.

Article Snippet: Briefly, 100 µL of the collected samples were spread on Difco™ m Enterococcus selective Agar (Thermo Fisher Scientific Australia Pty Ltd., Scoresby, VIC, Australia) and incubated at 37°C.

Techniques: Activity Assay, Concentration Assay, Control

Time-kill kinetics of Enterococcus bacteriophages. (a) Growth pattern of E. faecalis ATTC 700802 incubated with individual phages APTC-Efa.10, Efa.16 and Efa.20 (MOI) in the range between (0.01-100). (b) Growth pattern of E. faecalis ATTC 700802 incubated with 1:1 phage cocktail at different MOIs (0.01-100).

Journal: bioRxiv

Article Title: LicD-Mediated Cell Wall Decoration Governs Phage Sensitivity in Enterococcus faecalis Clinical Isolates

doi: 10.1101/2025.05.13.653012

Figure Lengend Snippet: Time-kill kinetics of Enterococcus bacteriophages. (a) Growth pattern of E. faecalis ATTC 700802 incubated with individual phages APTC-Efa.10, Efa.16 and Efa.20 (MOI) in the range between (0.01-100). (b) Growth pattern of E. faecalis ATTC 700802 incubated with 1:1 phage cocktail at different MOIs (0.01-100).

Article Snippet: Briefly, 100 µL of the collected samples were spread on Difco™ m Enterococcus selective Agar (Thermo Fisher Scientific Australia Pty Ltd., Scoresby, VIC, Australia) and incubated at 37°C.

Techniques: Incubation