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empty vector l4440  (Addgene inc)


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    Addgene inc empty vector l4440
    Empty Vector L4440, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 262 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+l4440/L4440+(Plasmid+%231654)/bio_rxiv__64898__2025__12__19__695459-458-0-3
    Average 95 stars, based on 262 article reviews
    empty vector l4440 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Bacteria:

    Article Title: Reducing translation through eIF 4G/ IFG ‐1 improves survival under ER stress that depends on heat shock factor HSF ‐1 in Caenorhabditis elegans
    Article Snippet: .. Bacteria expressing dsRNA included empty vector L4440 (Addgene, Cambridge, MA, U.S.A.), ifg‐1 (M110.4), sca‐1 (K11D9.2), mdt‐15 (R12B2.5), ire‐1 (C41C4.4), atf‐6 (F45E6.2), pek‐1 (F46C3.1), xbp‐1 (R74.3), hsf‐1 (Y53C10A.12), daf‐16 (R13H8.1), ife‐2 (R04A9.4) , rps‐15 (F36A2.6) , and iftb‐1 (K04G2.1) from the Ahringer library (Source BioScience, Nottingham, U.K.). ..

    Article Title: Anabolic Function Downstream of TOR Controls Trade-offs Between Longevity and Reproduction at the Level of Specific Tissues in C. elegans
    Article Snippet: .. RNAi bacteria strains included empty vector L4440, GFP: L4440 (11335) (Addgene, Cambridge, MA, United States), eif-2beta (K04G2.1), ifg-1 (M110.4), let-363 (B0261.2), unc-22 (ZK617.1) from the Ahringer library (Source BioScience, Nottingham, United Kingdom) and rict-1 (F29C12.3) from the Vidal library (Source Bioscience). daf-15 (C10C5.6) ( ) was a generous gift from the Han Lab (University of Colorado, Boulder). ..

    Article Title: Neuronal CBP-1 is required for enhanced body muscle proteostasis in response to reduced translation downstream of mTOR
    Article Snippet: .. RNAi bacteria strains included empty vector L4440 (Addgene, Cambridge, MA, USA), ifg-1 (M110.4), cbp-1 (R10E11.1), daf-15 (C10C5.6, gifted from Han Lab), and rict-1 (F29C12.3) (Ahringer library, Source BioScience, Nottingham, UK). ..

    Article Title: Neuronal CBP-1 is Required for Enhanced Body Muscle Proteostasis in Response to Reduced Translation Downstream of mTOR.
    Article Snippet: .. RNAi bacteria strains included empty vector L4440 (Addgene, Cambridge, MA, USA), ifg-1 (M110.4), cbp-1 (R10E11.1), daf-15 (C10C5.6, gifted from Han Lab), and rict-1 (F29C12.3) (Ahringer library, Source BioScience, Nottingham, UK). ..

    Article Title: Dietary restriction induces posttranscriptional regulation of longevity genes
    Article Snippet: .. Bacteria expressing dsRNA included: empty vector L4440 (Addgene), those from the Ahringer library or if not available, from the Vidal ORF library (Source BioScience). ..

    Article Title: Neuronal CBP-1 is Required for Enhanced Body Muscle Proteostasis in Response to Reduced Translation Downstream of mTOR
    Article Snippet: .. RNAi bacteria strains included empty vector L4440 (Addgene, Cambridge, MA, USA), ifg-1 (M110.4), cbp-1 (R10E11.1), daf-15 (C10C5.6, gifted from Han Lab), and rict-1 (F29C12.3) (Ahringer library, Source BioScience, Nottingham, UK). ..

    Expressing:

    Article Title: Reducing translation through eIF 4G/ IFG ‐1 improves survival under ER stress that depends on heat shock factor HSF ‐1 in Caenorhabditis elegans
    Article Snippet: .. Bacteria expressing dsRNA included empty vector L4440 (Addgene, Cambridge, MA, U.S.A.), ifg‐1 (M110.4), sca‐1 (K11D9.2), mdt‐15 (R12B2.5), ire‐1 (C41C4.4), atf‐6 (F45E6.2), pek‐1 (F46C3.1), xbp‐1 (R74.3), hsf‐1 (Y53C10A.12), daf‐16 (R13H8.1), ife‐2 (R04A9.4) , rps‐15 (F36A2.6) , and iftb‐1 (K04G2.1) from the Ahringer library (Source BioScience, Nottingham, U.K.). ..

    Article Title: Dietary restriction induces posttranscriptional regulation of longevity genes
    Article Snippet: .. Bacteria expressing dsRNA included: empty vector L4440 (Addgene), those from the Ahringer library or if not available, from the Vidal ORF library (Source BioScience). ..

    Plasmid Preparation:

    Article Title: Mimicking human riboflavin responsive neuromuscular disorders by silencing flad‐1 gene in C. elegans : Alteration of vitamin transport and cholinergic transmission
    Article Snippet: .. Empty vector L4440 was purchased from Addgene ( http://www.addgene.org ) and used to transform HT115 E. coli cells. ..

    Article Title: Proteolytic Control of an Auto-inhibitory Intrinsically Disordered Region Governs Small RNA Selectivity in Argonaute Proteins
    Article Snippet: .. Empty vector L4440 (Addgene plasmid #1654; gift from Andrew Fire) served as a negative control. ..

    Article Title: Dietary restriction induces posttranscriptional regulation of longevity genes
    Article Snippet: .. Bacteria expressing dsRNA included: empty vector L4440 (Addgene), those from the Ahringer library or if not available, from the Vidal ORF library (Source BioScience). ..

    Negative Control:

    Article Title: Proteolytic Control of an Auto-inhibitory Intrinsically Disordered Region Governs Small RNA Selectivity in Argonaute Proteins
    Article Snippet: .. Empty vector L4440 (Addgene plasmid #1654; gift from Andrew Fire) served as a negative control. ..



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    Components for CRISPR activation in C. elegans by bacterial delivery of sgRNAs. A , domain organization of the dCas9::VP64 fusion protein, including two nuclear localization signals (NLSs), and a FLAG- and HA-tag. B , schematic representation of the region upstream of a given transcription start site (TSS) from which promoter-specific sgRNAs are selected. C , full sequence of the two sgRNA expression cassettes in vector <t>L4440_BioBrick-sgRNA,</t> including BioBrick cloning sites and with individual features as indicated by color. D , Western blot analysis of total protein extracts from WT N2 nematodes versus dCas9::VP64 nematodes outcrossed x0, x2, or x4 (MIR249). The 171 kDa dCas9::VP64 protein (dC9V) is detected with a FLAG antibody in all dCas9::VP64 nematodes and an additional unspecific (unsp.) band is detected in all samples at 25 kDa. E – G , lifespan assay of WT versus dCas9::VP64 nematodes on OP50 bacteria ( E ), HT115 bacteria carrying the L4440 vector ( F ), or HT115 sgRNA scramble control (SCR) bacteria ( G ). p -values of C. elegans lifespan assays were determined by log-rank test. See <xref ref-type=Table S1 for detailed lifespan assay statistics. HA, hemagglutinin; sgRNA, single-guide RNA. " width="250" height="auto" />
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    Image Search Results


    Key Resources Table

    Journal: Current biology : CB

    Article Title: Centriole-less pericentriolar material serves as a microtubule organizing center at the base of C. elegans sensory cilia

    doi: 10.1016/j.cub.2021.03.022

    Figure Lengend Snippet: Key Resources Table

    Article Snippet: Plasmid: L4440: RNAi empty vector , Addgene , Cat#1654.

    Techniques: Recombinant, Mutagenesis, CRISPR, Sequencing, Plasmid Preparation, Software

    Components for CRISPR activation in C. elegans by bacterial delivery of sgRNAs. A , domain organization of the dCas9::VP64 fusion protein, including two nuclear localization signals (NLSs), and a FLAG- and HA-tag. B , schematic representation of the region upstream of a given transcription start site (TSS) from which promoter-specific sgRNAs are selected. C , full sequence of the two sgRNA expression cassettes in vector L4440_BioBrick-sgRNA, including BioBrick cloning sites and with individual features as indicated by color. D , Western blot analysis of total protein extracts from WT N2 nematodes versus dCas9::VP64 nematodes outcrossed x0, x2, or x4 (MIR249). The 171 kDa dCas9::VP64 protein (dC9V) is detected with a FLAG antibody in all dCas9::VP64 nematodes and an additional unspecific (unsp.) band is detected in all samples at 25 kDa. E – G , lifespan assay of WT versus dCas9::VP64 nematodes on OP50 bacteria ( E ), HT115 bacteria carrying the L4440 vector ( F ), or HT115 sgRNA scramble control (SCR) bacteria ( G ). p -values of C. elegans lifespan assays were determined by log-rank test. See <xref ref-type=Table S1 for detailed lifespan assay statistics. HA, hemagglutinin; sgRNA, single-guide RNA. " width="100%" height="100%">

    Journal: The Journal of Biological Chemistry

    Article Title: Ingestion of single guide RNAs induces gene overexpression and extends lifespan in Caenorhabditis elegans via CRISPR activation

    doi: 10.1016/j.jbc.2022.102085

    Figure Lengend Snippet: Components for CRISPR activation in C. elegans by bacterial delivery of sgRNAs. A , domain organization of the dCas9::VP64 fusion protein, including two nuclear localization signals (NLSs), and a FLAG- and HA-tag. B , schematic representation of the region upstream of a given transcription start site (TSS) from which promoter-specific sgRNAs are selected. C , full sequence of the two sgRNA expression cassettes in vector L4440_BioBrick-sgRNA, including BioBrick cloning sites and with individual features as indicated by color. D , Western blot analysis of total protein extracts from WT N2 nematodes versus dCas9::VP64 nematodes outcrossed x0, x2, or x4 (MIR249). The 171 kDa dCas9::VP64 protein (dC9V) is detected with a FLAG antibody in all dCas9::VP64 nematodes and an additional unspecific (unsp.) band is detected in all samples at 25 kDa. E – G , lifespan assay of WT versus dCas9::VP64 nematodes on OP50 bacteria ( E ), HT115 bacteria carrying the L4440 vector ( F ), or HT115 sgRNA scramble control (SCR) bacteria ( G ). p -values of C. elegans lifespan assays were determined by log-rank test. See Table S1 for detailed lifespan assay statistics. HA, hemagglutinin; sgRNA, single-guide RNA.

    Article Snippet: For the expression of C. elegans promoter-specific sgRNAs in E . coli HT115 bacteria, the L4440 RNAi empty vector (Addgene; plasmid #1654) was modified to contain two consecutive sgRNA expression cassettes ( , ) under control of individual T7 promoters and together flanked by BioBrick cloning sites ( ) ( C ), resulting in the vector L4440_BioBrick-sgRNA.

    Techniques: CRISPR, Activation Assay, Sequencing, Expressing, Plasmid Preparation, Clone Assay, Western Blot