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Greiner Bio high binding elisa plates

(A) Sera from MRL lpr mice (n=12, bled weekly from 8 to 13 weeks of age) were analyzed by <t>ELISA</t> using synthetic 10×RG peptides: native sequence (GRGRGRGRGRGRGRGRGRGR) vs. SDMA-modified (lowercase ‘r’ denotes SDMA; GrGrGrGRGRGrGrGrGRGR). All 12 mice developed anti SDMA antibody titers above the corresponding native controls, and the majority (9/12) showed a gradual increase in anti-SDMA titers over time. (B) Immunoblotting of HEp-2 lysates revealed progressive changes in band patterns from 10 weeks onward. To identify SDMA specific reactive proteins, cells were pretreated with the methyltransferase inhibitor EPZ015666 (EPZ) before lysis. A prominent ∼15 kDa band (arrowhead) was markedly reduced by EPZ pretreatment, supporting its recognition by anti SDMA antibodies. (C) Sera from three 12 week old MRL lpr littermates (Lpr 1, 2, 3) each showed distinct band patterns against the cell lysates. EPZ pretreatment of the cells reduced the intensity of several bands in all three mice. (D) Workflow for isolating hybridoma clones from individual plasma cells to obtain monoclonal antibodies (mAbs) specific for SDMA. MRL lpr mice were not immunized with any antigen; thus, the resulting mAbs represent naturally arising autoantibodies. (E) Two antibody clones were tested against the native versus SDMA peptide array shown in Figurè3. Clone P4A2F4 reacted extensively with most SDMA containing peptides, whereas clone P2C1C10 showed no reactivity. (F) The two mAbs produced different band patterns on cell lysates. P4A2F4 recognized bands that were reduced in EPZ pretreated lysates, consistent with SDMA specificity. (G) After implantation of the SDMA-reactive hybridoma clone into the peritoneal cavity of healthy BALB/c mice, the kidneys showed extensive IgG deposition, reminiscent of human lupus nephritis.

High Binding Elisa Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 97/100, based on 614 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/elisa+plate/Microplate+96+Well+Ps+F-Bottom/bio_rxiv__64898__2026__09__22__753525-203-0-3
Average 97 stars, based on 614 article reviews

high binding elisa plates - by Bioz Stars, 2026-10

97/100 stars

Images

1) Product Images from "Symmetric Dimethylarginine Modification Drives Shared and Divergent Antigen Reactivity in Lupus"

Article Title: Symmetric Dimethylarginine Modification Drives Shared and Divergent Antigen Reactivity in Lupus

Journal: bioRxiv

doi: 10.64898/2026.09.22.753525

(A) Sera from MRL lpr mice (n=12, bled weekly from 8 to 13 weeks of age) were analyzed by ELISA using synthetic 10×RG peptides: native sequence (GRGRGRGRGRGRGRGRGRGR) vs. SDMA-modified (lowercase ‘r’ denotes SDMA; GrGrGrGRGRGrGrGrGRGR). All 12 mice developed anti SDMA antibody titers above the corresponding native controls, and the majority (9/12) showed a gradual increase in anti-SDMA titers over time. (B) Immunoblotting of HEp-2 lysates revealed progressive changes in band patterns from 10 weeks onward. To identify SDMA specific reactive proteins, cells were pretreated with the methyltransferase inhibitor EPZ015666 (EPZ) before lysis. A prominent ∼15 kDa band (arrowhead) was markedly reduced by EPZ pretreatment, supporting its recognition by anti SDMA antibodies. (C) Sera from three 12 week old MRL lpr littermates (Lpr 1, 2, 3) each showed distinct band patterns against the cell lysates. EPZ pretreatment of the cells reduced the intensity of several bands in all three mice. (D) Workflow for isolating hybridoma clones from individual plasma cells to obtain monoclonal antibodies (mAbs) specific for SDMA. MRL lpr mice were not immunized with any antigen; thus, the resulting mAbs represent naturally arising autoantibodies. (E) Two antibody clones were tested against the native versus SDMA peptide array shown in Figurè3. Clone P4A2F4 reacted extensively with most SDMA containing peptides, whereas clone P2C1C10 showed no reactivity. (F) The two mAbs produced different band patterns on cell lysates. P4A2F4 recognized bands that were reduced in EPZ pretreated lysates, consistent with SDMA specificity. (G) After implantation of the SDMA-reactive hybridoma clone into the peritoneal cavity of healthy BALB/c mice, the kidneys showed extensive IgG deposition, reminiscent of human lupus nephritis.

Figure Legend Snippet: (A) Sera from MRL lpr mice (n=12, bled weekly from 8 to 13 weeks of age) were analyzed by ELISA using synthetic 10×RG peptides: native sequence (GRGRGRGRGRGRGRGRGRGR) vs. SDMA-modified (lowercase ‘r’ denotes SDMA; GrGrGrGRGRGrGrGrGRGR). All 12 mice developed anti SDMA antibody titers above the corresponding native controls, and the majority (9/12) showed a gradual increase in anti-SDMA titers over time. (B) Immunoblotting of HEp-2 lysates revealed progressive changes in band patterns from 10 weeks onward. To identify SDMA specific reactive proteins, cells were pretreated with the methyltransferase inhibitor EPZ015666 (EPZ) before lysis. A prominent ∼15 kDa band (arrowhead) was markedly reduced by EPZ pretreatment, supporting its recognition by anti SDMA antibodies. (C) Sera from three 12 week old MRL lpr littermates (Lpr 1, 2, 3) each showed distinct band patterns against the cell lysates. EPZ pretreatment of the cells reduced the intensity of several bands in all three mice. (D) Workflow for isolating hybridoma clones from individual plasma cells to obtain monoclonal antibodies (mAbs) specific for SDMA. MRL lpr mice were not immunized with any antigen; thus, the resulting mAbs represent naturally arising autoantibodies. (E) Two antibody clones were tested against the native versus SDMA peptide array shown in Figurè3. Clone P4A2F4 reacted extensively with most SDMA containing peptides, whereas clone P2C1C10 showed no reactivity. (F) The two mAbs produced different band patterns on cell lysates. P4A2F4 recognized bands that were reduced in EPZ pretreated lysates, consistent with SDMA specificity. (G) After implantation of the SDMA-reactive hybridoma clone into the peritoneal cavity of healthy BALB/c mice, the kidneys showed extensive IgG deposition, reminiscent of human lupus nephritis.

Techniques Used: Enzyme-linked Immunosorbent Assay, Sequencing, Modification, Western Blot, Lysis, Clone Assay, Clinical Proteomics, Bioprocessing, Autoantibodies, Peptide Microarray, Produced

Enzyme-linked Immunosorbent Assay:

Article Title: Probing Cell-Surface Interactions in Fungal Cell Walls by High-Resolution 1H-detected Solid-State NMR Spectroscopy
Article Snippet: .. Fungal growth of S. commune after 144 hours in an ELISA plate (Greiner Bio-one F bottom with lid) is shown. ..

Article Title: The Uptake of Heparanase into Mast Cells Is Regulated by Its Enzymatic Activity to Degrade Heparan Sulfate
Article Snippet: .. Briefly, streptavidin was immobilized onto the ELISA plate (Greiner, Frickenhausen, Germany, 655061). ..

Article Title: Influence of a pro-inflammatory stimulus on the miRNA and lipid content of human dental stem cell-derived extracellular vesicles and their impact on microglial activation
Article Snippet: .. Fifty μL of each SEC fraction [ , , , , , , , , , , , , , , , , , , , , , , , , , , , , , ] were bound to protein-binding ELISA plate (ELISA Strip Plate, 771,261; Greiner Bio-One, Frickenhausen, Germany). ..

Article Title: "Redirecting an anti-IL-1β antibody to bind a new, unrelated and computationally predicted epitope on hIL-17A".
Article Snippet: .. 50 μl of 5 nM hIL-17A was coated on the ELISA plate (Greiner Bio-One, Germany) for 1 h. Afterwards, the plate was washed 3× with 300 μl PBS-T/well, and the wells were blocked with PBS 1% BSA, and then incubated with 50ul of indicated antibody at a concentration range of 0 to 200 nM for 1 h. Subsequently, 50 μl of 25 nM of IL17RA was added for 15 min and washed again three times. .. Secondary anti His HRP conjugated antibody (Santa Cruz Biotechnology, USA, cat: sc8036 HRP) diluted 1:250 in PBS-T was added for an incubation of 20 min at RT.

Article Title: High‐molecular‐weight oligomer tau (<scp>HMWoTau</scp>) species are dramatically increased in Braak‐stage dependent manner in the frontal lobe of human brains, demonstrated by a novel oligomer Tau <scp>ELISA</scp> with a mouse monoclonal antibody (<scp>APNmAb005</scp>)
Article Snippet: .. Firstly, ELISA plate (Fluotrac 96- well black plate, high- binding, Greiner, Cat. No. 655077) or (Maxisorp 96- well immune standard modules, black plate, Nunc, Cat. No. 475515) was coated with monoclonal antibodies of mAb005 (1–5 μg/mL), mAb004 (1–5 μg/mL), or HT7 (1 μg/ mL) at a volume of 100 μL/well in coating buffer A (carbonate buffer, pH 9.6, 100 mM). .. After washing plates with PBS(−) using plate washer (AMW- 3 Auto Mini Washer, Bio Tec, Japan), each well was blocked with 120 μL of 1% (W/V) BlockAce (KAC Co. Ltd., Cat. No. UKB40, Japan) solution in PBS(−), pH 7.4.

Article Title: Molecular insights into septin 2 protein in rohu (Labeo rohita): revealing expression dynamics, antimicrobial activity and functional characteristics.
Article Snippet: Septins are evolutionarily conserved GTP-binding proteins mediating innate immunity, autophagy and inflammation in higher animals; however, they are yet to be fully characterized in fish.. The study encompasses cloning of complete septin 2 cDNA from the rohu carp (Labeo rohita) that consisted of an open reading frame of 1050 bp and phylogenetic amino acid similarity of 99.43 % to cyprinid Onychostoma macrolepis.. Septin 2 was ubiquitously expressed in different tissues of healthy rohu, and during early developmental stages.

Article Title: A Single-Molecule Bioelectronic Portable Array for Early Diagnosis of Pancreatic Cancer Precursors.
Article Snippet: .. As a final step, a standard black bottomless ELISA plate with 96 wells (Greiner Bio-One) was glued over the plastic foil with a medicalgrade double-sided adhesive foil. ..

Protein Binding:

Stripping Membranes:

Incubation:

Concentration Assay:

Binding Assay:

Bioprocessing:

other:

Article Title: The extracellular matrix integrates mitochondrial homeostasis.
Article Snippet: Article The extracellular matrix integrates mitochondrial homeostasis

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