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electrodes per well  (Applied BioPhysics)


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    Applied BioPhysics electrodes per well
    Electrodes Per Well, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 95/100, based on 441 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/electrodes+per+well/8W10E+PET/pm41507584-337-13-17
    Average 95 stars, based on 441 article reviews
    electrodes per well - by Bioz Stars, 2026-11
    95/100 stars

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    other:

    Article Title: 2′-Fucosyllactose impacts the expression of mucus-related genes in goblet cells and maintains barrier function of gut epithelial cells
    Article Snippet: T84 cells were grown in 96-well array plates with 20 electrodes per well (96W20idfPET, Ibidi via Applied Biophysics).

    Positron Emission Tomography:

    Article Title: Uropathogenic Escherichia coli invade luminal prostate cells via FimH-PPAP receptor binding.
    Article Snippet: An ECIS 8-well transfilter array connected to ECIS Z-Theta (Applied Biophysics) was used to measure the TEER of the organoid-based model. .. Cells were seeded as described above on an 8-well PET slide with 40 electrodes per well (8W10E+, Applied Biophysics). .. At the indicated time, organoid-infected cells were washed with PBS and lysed with 0.1% Triton X-100 (T9284, Sigma-Aldrich) in PBS.

    Article Title: Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid-derived monolayers compared to HeLa cells.
    Article Snippet: .. To evaluate TEER, cells were seeded on 8-well PET slides with 40 electrodes per well (8W10E+, Applied Biophysics, Troy, NY, USA). .. To evaluate TEER, cells were seeded on 8-well PET slides with 40 electrodes per well (8W10E+, Applied Biophysics, Troy, NY, USA).

    Article Title: Uropathogenic Escherichia coli invade luminal prostate cells via FimH–PPAP receptor binding
    Article Snippet: An ECIS 8-well transfilter array connected to ECIS Z-Theta (Applied Biophysics) was used to measure the TEER of the organoid-based model. .. Cells were seeded as described above on an 8-well PET slide with 40 electrodes per well (8W10E+, Applied Biophysics). ..

    Article Title: BMP9 (Bone Morphogenetic Protein-9)/Alk1 (Activin-Like Kinase Receptor Type I) Signaling Prevents Hyperglycemia-Induced Vascular Permeability
    Article Snippet: Endothelial barrier function analysis was achieved using electric cellsubstrate impedance sensing system (ECIS Zθ; Applied Biophysics). .. HUVECs and HRMECs were seeded on collagen-coated electric cellsubstrate impedance sensing system arrays each holding 8 wells with 10 electrodes per well (8W10E PET; Applied Biophysics). ..

    Electric Cell-substrate Impedance Sensing:

    Article Title: MAPK Signaling and Angiopoietin-2 Contribute to Endothelial Permeability in Capillary Malformations
    Article Snippet: .. 30,000 cells/well were seeded on a fibronectin-coated (0.1mg/cm 2 ) 96 well array with 10 interdigitated electrodes per well (Applied BioPhysics, 96W10idfPET ECIS array) in EGM2. .. Once cells adhered and formed a monolayer overnight (16hours), media was switched to reduced serum (2% FBS) EBM2 supplemented with thrombin (0.5 U/ml) (Sigma-Aldrich, 605190) to disrupt the nascent EC-EC contacts and follow subsequent barrier formation measured by electrical resistance (Ω).

    Article Title: CD45 Is Sufficient to Initiate Endothelial-to-Mesenchymal Transition in Human Endothelial Cells—Brief Report
    Article Snippet: e124 May 2023 Arterioscler Thromb Vasc Biol.. 2023;43:e124–e131.. DOI: 10.1161/ATVBAHA.122.318172 Arterioscler Thromb Vasc Biol is available at www.ahajournals.org/journal/atvb Correspondence to: Joyce Bischoff, PhD, Karp Family Research Laboratories 12.212, Boston Children’s Hospital, 300 Longwood Ave, Boston, MA 02115.

    Article Title: MAPK signaling and angiopoietin-2 contribute to endothelial permeability in capillary malformations.
    Article Snippet: .. Trans Endothelial Electrical Resistance (TEER) Assay First, 30,000 cells/well were seeded on a fibronectin- coated (0.1 mg/cm2) 96- well array with 10 interdigitated electrodes per well (Applied BioPhysics, 96W10idfPET ECIS array) in EGM2. .. Once cells adhered and formed a monolayer overnight (16 h), medium was replaced with reduced serum (2% FBS) EBM2 supplemented with thrombin (0.5 U/mL) (Sigma- Aldrich, 605190) to disrupt the nascent EC–EC contacts and follow subsequent barrier formation measured by electrical resistance (Ω).



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    Validation of neuronal integrity. (A) Viability assay of iSNs following treatment with increasing concentrations of FdU and AraC for up to 72 h. SDS was used as negative ctrl, 10 μM of FdU for 24 h. The data of n = 3 differentiations, one-way ANOVA with post-hoc Tukey's HSD. * p < 0.05; *** p < 0.001. (B) iSNs generated using our optimized protocol express the sensory neuron marker Nav1.8, the peripheral neuron marker PRPH, and a general neuronal marker TUJ1. The scale bar = 100 μm. (C, D) Spontaneous electrical activity in iSNs was measured at 4 (I), 5 (II), and 6 (III) weeks of maturation using a <t>multi-electrode</t> array system, with activity detectable starting from 4 weeks of maturation. Scale: 50 μV/10 s. (D) Raster plot of one well over 4 (I), 5 (II), and 6 (III) weeks of maturation showing the single and developing network activity. For (C, D) n = 3 triplicates from 1 differentiation. ICC, immunocytochemistry; Nav1.8, voltage-gated sodium channel 1.8; PRPH, peripherin; TUJ1, Beta-III-tubulin.
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    Validation of neuronal integrity. (A) Viability assay of iSNs following treatment with increasing concentrations of FdU and AraC for up to 72 h. SDS was used as negative ctrl, 10 μM of FdU for 24 h. The data of n = 3 differentiations, one-way ANOVA with post-hoc Tukey's HSD. * p < 0.05; *** p < 0.001. (B) iSNs generated using our optimized protocol express the sensory neuron marker Nav1.8, the peripheral neuron marker PRPH, and a general neuronal marker TUJ1. The scale bar = 100 μm. (C, D) Spontaneous electrical activity in iSNs was measured at 4 (I), 5 (II), and 6 (III) weeks of maturation using a multi-electrode array system, with activity detectable starting from 4 weeks of maturation. Scale: 50 μV/10 s. (D) Raster plot of one well over 4 (I), 5 (II), and 6 (III) weeks of maturation showing the single and developing network activity. For (C, D) n = 3 triplicates from 1 differentiation. ICC, immunocytochemistry; Nav1.8, voltage-gated sodium channel 1.8; PRPH, peripherin; TUJ1, Beta-III-tubulin.

    Journal: Frontiers in Neuroscience

    Article Title: Human sensory-like neuron cultivation—An optimized protocol

    doi: 10.3389/fnins.2024.1429694

    Figure Lengend Snippet: Validation of neuronal integrity. (A) Viability assay of iSNs following treatment with increasing concentrations of FdU and AraC for up to 72 h. SDS was used as negative ctrl, 10 μM of FdU for 24 h. The data of n = 3 differentiations, one-way ANOVA with post-hoc Tukey's HSD. * p < 0.05; *** p < 0.001. (B) iSNs generated using our optimized protocol express the sensory neuron marker Nav1.8, the peripheral neuron marker PRPH, and a general neuronal marker TUJ1. The scale bar = 100 μm. (C, D) Spontaneous electrical activity in iSNs was measured at 4 (I), 5 (II), and 6 (III) weeks of maturation using a multi-electrode array system, with activity detectable starting from 4 weeks of maturation. Scale: 50 μV/10 s. (D) Raster plot of one well over 4 (I), 5 (II), and 6 (III) weeks of maturation showing the single and developing network activity. For (C, D) n = 3 triplicates from 1 differentiation. ICC, immunocytochemistry; Nav1.8, voltage-gated sodium channel 1.8; PRPH, peripherin; TUJ1, Beta-III-tubulin.

    Article Snippet: The electrical activity of iSNs was assessed using a multiwell multi-electrode array (MEA) system comprising 24 individual wells with 12 electrodes per well (Multi Channel Systems MCS GmbH, Reutlingen, Germany).

    Techniques: Viability Assay, Generated, Marker, Activity Assay, Immunocytochemistry