Journal: Cell Stress & Chaperones

Article Title: Inhibition of α-1,3-mannosyltransferase sensitizes head and neck squamous cell carcinomas to cetuximab via endoplasmic reticulum stress

doi: 10.1016/j.cstres.2026.100153

Figure Lengend Snippet: Inhibition of ALG3-induced ER stress in HNSCC cells. (a) and (b) Relative expression of IRE1α, BiP, CHOP, and ATF6 mRNAs in the indicated groups ( n = 3). (c) and (d) Immunoblot showing relative expression of PERK, IRE1α, Bip, CHOP, and ATF6 proteins in the indicated groups ( n = 3). The right panels showing densitometric quantification of IRE1α and Bip expression from three independent biological replicates, normalized to GAPDH. (e) and (f) Representative TEM images showing changes in the ER (red rectangle) of Ctrl KD and ALG3 KD HNSCC cells. Scale bar, 200 nm. The HNSCC cells were treated with 0.1 µg/mL tunicamycin (Tun) or vehicle for 24 h. ns P ≥ 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The membrane blots were probed with primary antibodies against ATF4 (60035-1-Ig, Proteintech), ATF6 (24169-1-AP, Proteintech), CHOP (15204-1-AP, Proteintech), IRE1α (#3294, Cell Signaling Technology), PERK (24390-1-AP, Proteintech), phospho-PERK (81251-2-RR, Proteintech), EGFR (ET1604-44, HUABIO), and GAPDH (60004-1-Ig, Proteintech), followed by horseradish peroxidase-conjugated anti-rabbit IgG antibodies.

Techniques: Inhibition, Expressing, Western Blot

Journal: Journal of Advanced Research

Article Title: Squalene epoxidase promotes paraquat-induced pulmonary toxicity through endoplasmic reticulum-mediated ferroptosis

doi: 10.1016/j.jare.2025.05.064

Figure Lengend Snippet: ER stress mediates PQ-induced ferroptosis. (A-B) A549 cells were transfected with siRNA of PERK or NC (negative control) and then treated with PQ for 24 h. The protein expression (A) and the cell viability were determined (B). (C) Western blotting analysis of p-PERK, PERK, p-eIF2α, eIF2α, and CHOP in A549 cells pre-treated with 1 μM GSK157 for 1 h followed by PQ (1000 μM) treatment for 24 h. (D-E). A549 cells pre-treated with GSK157 (0.25, 0.5, 1 μM) for 1 h followed by PQ (1000 μM) treatment for 24 h, then ATP release (D) and CCK-8 assay (E) were determined. (F) Effects of GSK157 on PQ-induced lipid peroxidation. A549 Cells were pretreated with GSK157 for 1 h and then exposed to PQ for 24 h, followed by C11-BODIPY staining and determined by flow cytometry analysis. (G) Effects of GSK157 on PQ-induced iron content elevation. Cells were pretreated with GSK157 for 1 h and then exposed to PQ for 24 h, followed by calcein-AM staining and determined by flow cytometry analysis. (H) The staining of ER-Tracker (green) and FerroOrange (red) with Hoechst (blue) was photographed by the confocal microscope (Bar = 10 μm). (I) Iron content in A549 cells. (J) Effect of RSL3 on PQ-induced ER-stress. Cells were treated with PQ (1000 μM) for 24 h in the presence or absence of RSL3 (1 μM) and GSK157 (1 μM) pretreatment for 1 h. CCK-8 assay. (K) The expression levels of GPX4 and SLC7A11 were determined by Western blotting. Data are presented as Mean ± S.D. of at least three independent biological replicates (n = 3). *P < 0.05 , ** P < 0.01 , *** P < 0.001 show significant differences from each group. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Antibodies for SQLE (#67206–1-Ig), GPX4 (#67763–1-Ig), solute carrier family 7 member 11 (SLC7A11, #26864–1-AP), and PERK (#20582–1-AP) were purchased from Proteintech Group, Inc. (Wuhan, China).

Techniques: Transfection, Negative Control, Expressing, Western Blot, CCK-8 Assay, Staining, Flow Cytometry, Microscopy