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cells with anti egfr af488  (R&D Systems)


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    R&D Systems cells with anti egfr af488
    Cells With Anti Egfr Af488, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egfr+af488/Human+EGFR+(Research+Grade+Cetuximab+Biosimilar)+Alexa+Fluor%C2%AE+488-conjugated+Antibody/pmc12804178-97-4-7
    Average 94 stars, based on 16 article reviews
    cells with anti egfr af488 - by Bioz Stars, 2026-10
    94/100 stars

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    Staining:

    Article Title: CX3CR1 deficiency-induced TIL tumor restriction as a novel addition for CAR-T design in solid malignancies
    Article Snippet: CAR-T were characterized in a panel containing: EGFR AF488 (R&D Systems catalog number FAB9577G), CD4 PerCP-Cy5.5 (BD Biosciences catalog number 560650, RRID: AB_1727476 ), CX3CR1 APC (Thermo Fisher Scientific catalog number 17-6099-42, RRID: AB_11149136 ), CD28 APC-H7 (BD Biosciences catalog number 561368, RRID: AB_11154032 ), PD1 BV421 (BD Biosciences catalog number 562516, RRID: AB_11153482 ), CD127 BV605 (BD Biosciences catalog number 562662, RRID: AB_2737706 ), CD62L BV711 (BD Biosciences catalog number 565040, RRID: AB_2869642 ), Tim3 BV786 (BD Biosciences catalog number 742857, RRID: AB_2741100 ), CCR7 BUV395 (Thermo Fisher Scientific catalog number 17-6099-42, RRID: AB_11149136 ), TIGIT APC-Fire750 (BioLegend catalog number 372708, RRID: AB_2632755 ), CTLA-4 PE-CF594 (BD Biosciences catalog number 562742, RRID: AB_2737761 ), CD8 BUV496 (BD Biosciences catalog number 564804, RRID: AB_2744460 ), CD27 BUV 737 (BD Biosciences catalog number 564301, RRID: AB_2744350 ), CD3 BUV605 (BD Biosciences catalog number 564712, RRID: AB_2738908 ), LAG3 APC-R700 (BD Biosciences catalog number 565774, RRID: AB_2744329 ), CD95 PE-CF594 (BD Biosciences catalog number 562395, RRID: AB_11153666 ), and CD45RA PE-Cy7 (BD Biosciences catalog number 560675, RRID: AB_1727498 ). .. Mouse blood samples were collected weekly and stained with the following anti-human antibodies: EGFR AF488 (R&D systems catalog number FAB9577G), CX3CR1 APC (Thermo Fisher Scientific catalog number 17-6099-42, RRID: AB_11149136 ), CD45 BUV805 (BD Biosciences catalog number 612891, RRID: AB_2870179 ), Live/Dead Fixable Aqua, and NKG2D PE-Cy7 (BD Biosciences catalog number 562365, RRID: AB_11153309 ). .. Flow cytometry was performed on a BD FACSymphony at the MCC Flow Cytometry Core Facility and analyzed with FlowJo software (FlowJo, RRID: SCR_008520 ). tSNE plots were made with FlowJo by first normalizing down each sample to create each sample with the same number of events and then creating a concatenated group specimen.

    Article Title: CX3CR1 deficiency-induced TIL tumor restriction as a novel addition for CAR-T design in solid malignancies.
    Article Snippet: CAR-T were characterized in a panel containing: EGFR AF488 (R&D Systems catalog number FAB9577G), CD4 PerCP-Cy5.5 (BD Biosciences catalog number 560650, RRID:AB_1727476), CX3CR1 APC (Thermo Fisher Scientific catalog number 17-6099-42, RRID:AB_11149136), CD28 APC-H7 (BD Biosciences catalog number 561368, RRID:AB_11154032), PD1 BV421 (BD Biosciences catalog number 562516, RRID:AB_11153482), CD127 BV605 (BD Biosciences catalog number 562662, RRID:AB_2737706), CD62L 20 iScience 26, 106443, April 21, 2023 BV711 (BD Biosciences catalog number 565040, RRID:AB_2869642), Tim3 BV786 (BD Biosciences catalog number 742857, RRID:AB_2741100), CCR7 BUV395 (Thermo Fisher Scientific catalog number 17-6099-42, RRID:AB_11149136), TIGIT APC-Fire750 (BioLegend catalog number 372708, RRID:AB_2632755), CTLA-4 PE-CF594 (BD Biosciences catalog number 562742, RRID:AB_2737761), CD8 BUV496 (BD Biosciences catalog number 564804, RRID:AB_2744460), CD27 BUV 737 (BD Biosciences catalog number 564301, RRID:AB_2744350), CD3 BUV605 (BD Biosciences catalog number 564712, RRID:AB_2738908), LAG3 APC-R700 (BD Biosciences catalog number 565774, RRID:AB_2744329), CD95 PE-CF594 (BD Biosciences catalog number 562395, RRID:AB_11153666), and CD45RA PE-Cy7 (BD Biosciences catalog number 560675, RRID:AB_1727498). .. Mouse blood samples were collected weekly and stained with the following anti-human antibodies: EGFR AF488 (R&D systems catalog number FAB9577G), CX3CR1 APC (Thermo Fisher Scientific catalog number 17-6099-42, RRID:AB_11149136), CD45 BUV805 (BD Biosciences catalog number 612891, RRID:AB_ 2870179), Live/Dead Fixable Aqua, and NKG2D PE-Cy7 (BD Biosciences catalog number 562365, RRID:AB_11153309). .. Flow cytometry was performed on a BD FACSymphony at the MCC Flow Cytometry Core Facility and analyzed with FlowJo software (FlowJo, RRID:SCR_008520). tSNE plots were made with FlowJo by first normalizing down each sample to create each sample with the same number of events and then creating a concatenated group specimen.

    other:

    Article Title: CX3CR1 deficiency-induced TIL tumor restriction as a novel addition for CAR-T design in solid malignancies.
    Article Snippet: Flow cytometry was performed on a BD FACSymphony at the MCC Flow Cytometry Core Facility and analyzed with FlowJo software (FlowJo, RRID:SCR_008520). tSNE plots were made with FlowJo by first normalizing down each sample to create each sample with the same number of events and then creating a concatenated group specimen.



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    Key resources table
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    Key resources table

    Journal: iScience

    Article Title: CX3CR1 deficiency-induced TIL tumor restriction as a novel addition for CAR-T design in solid malignancies

    doi: 10.1016/j.isci.2023.106443

    Figure Lengend Snippet: Key resources table

    Article Snippet: CAR-T were characterized in a panel containing: EGFR AF488 (R&D Systems catalog number FAB9577G), CD4 PerCP-Cy5.5 (BD Biosciences catalog number 560650, RRID: AB_1727476 ), CX3CR1 APC (Thermo Fisher Scientific catalog number 17-6099-42, RRID: AB_11149136 ), CD28 APC-H7 (BD Biosciences catalog number 561368, RRID: AB_11154032 ), PD1 BV421 (BD Biosciences catalog number 562516, RRID: AB_11153482 ), CD127 BV605 (BD Biosciences catalog number 562662, RRID: AB_2737706 ), CD62L BV711 (BD Biosciences catalog number 565040, RRID: AB_2869642 ), Tim3 BV786 (BD Biosciences catalog number 742857, RRID: AB_2741100 ), CCR7 BUV395 (Thermo Fisher Scientific catalog number 17-6099-42, RRID: AB_11149136 ), TIGIT APC-Fire750 (BioLegend catalog number 372708, RRID: AB_2632755 ), CTLA-4 PE-CF594 (BD Biosciences catalog number 562742, RRID: AB_2737761 ), CD8 BUV496 (BD Biosciences catalog number 564804, RRID: AB_2744460 ), CD27 BUV 737 (BD Biosciences catalog number 564301, RRID: AB_2744350 ), CD3 BUV605 (BD Biosciences catalog number 564712, RRID: AB_2738908 ), LAG3 APC-R700 (BD Biosciences catalog number 565774, RRID: AB_2744329 ), CD95 PE-CF594 (BD Biosciences catalog number 562395, RRID: AB_11153666 ), and CD45RA PE-Cy7 (BD Biosciences catalog number 560675, RRID: AB_1727498 ).

    Techniques: Marker, Recombinant, Enzyme-linked Immunosorbent Assay, Cell Isolation, Activation Assay, Staining, Over Expression, Construct, Plasmid Preparation, Software, Microscopy, Imaging, Flow Cytometry