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plasmid vectors osmrβ: prp[exp]-egfp/neo-cag>mosmr[ nm_011019.3 ], control: prp[exp]-egfp/neo-cag>orf stuffer  (VectorBuilder GmbH)

 
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    VectorBuilder GmbH plasmid vectors osmrβ: prp[exp]-egfp/neo-cag>mosmr[ nm_011019.3 ], control: prp[exp]-egfp/neo-cag>orf stuffer
    Plasmid Vectors Osmrβ: Prp[Exp] Egfp/Neo Cag>Mosmr[ Nm 011019.3 ], Control: Prp[Exp] Egfp/Neo Cag>Orf Stuffer, supplied by VectorBuilder GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egfp+vector+control/plasmid+vectors+osmr%CE%B2++prp+exp++egfp+neo+cag+mosmr++nm+011019+3++++control++prp+exp++egfp+neo+cag+orf+stuffer/pmc12267034-48-7-12
    Average 90 stars, based on 1 article reviews
    plasmid vectors osmrβ: prp[exp]-egfp/neo-cag>mosmr[ nm_011019.3 ], control: prp[exp]-egfp/neo-cag>orf stuffer - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: NAC-mediated ribosome localization regulates cell fate and metabolism in intestinal stem cells
    Article Snippet: The scramble shRNA lentiviral control vector pLV[shRNA]-EGFP/Puro-U6>Scramble_shRNA (VB010000-0009mxc, VectorBuilder) was used as a control.

    Article Title: Targeting WEE1 enhances the antitumor effect of KRAS-mutated non-small cell lung cancer harboring TP53 mutations.
    Article Snippet: Generation of cDNA-expressing cell lines The following vectors were purchased from VectorBuilder: pLV[Exp]-EGFP:T2A:Puro-EF1A > hTP53[NM_000546.5], the control vector pLV[Exp]-EGFP:Puro-EF1A > ORF_stuffer, pLV[Exp]-mCherry:T2A:Hygro-EF1A > hCHEK2[NM_001005735.2], and the control vector pLV[Exp]-mCherry/Hygro-EF1A > ORF_stuffer.

    Article Title: Targeting WEE1 enhances the antitumor effect of KRAS- mutated non-small cell lung cancer harboring TP53 mutations
    Article Snippet: The following vectors were purchased from VectorBuilder: pLV[Exp]-EGFP:T2A:Puro-EF1A > hTP53[NM_000546.5], the control vector pLV[Exp]-EGFP:Puro-EF1A > ORF_stuffer, pLV[Exp]-mCherry:T2A:Hygro-EF1A > hCHEK2[NM_001005735.2], and the control vector pLV[Exp]-mCherry/Hygro-EF1A > ORF_stuffer.

    Article Title: NAC regulates metabolism and cell fate in intestinal stem cells.
    Article Snippet: The scramble shRNA lentiviral control vector pLV[shRNA]- EGFP/Puro- U6 > Scramble_shRNA (VB010000- 0009mxc, VectorBuilder) was used as a control.

    Article Title: NAC regulates metabolism and cell fate in intestinal stem cells
    Article Snippet: The scramble shRNA lentiviral control vector pLV[shRNA]-EGFP/Puro-U6 > Scramble_shRNA (VB010000-0009mxc, VectorBuilder) was used as a control.

    Control:

    Article Title: Dynamics of the CD9 interactome during bacterial infection of epithelial cells by proximity labelling proteomics
    Article Snippet: .. An eGFP lentiviral control vector (VB010000-9389rbj; Vectorbuilder) was used throughout the study. ..

    Article Title: Sestrin2 ameliorates age-related spontaneous benign prostatic hyperplasia via activation of AMPK/mTOR dependent autophagy.
    Article Snippet: therapeutic role in spontaneous BPH.. In vivo study, the 3-month-old (3 M) and 24-month-old (24 M) mice were used, and the 24 M mice were additionally administered recombinant Sestrin2 protein (rp-Sestrin2) for consecutive 14 days.. In vitro, BPH-1 cells were transfected with an empty or Sestrin2 overexpression vector.

    Plasmid Preparation:

    Article Title: Dynamics of the CD9 interactome during bacterial infection of epithelial cells by proximity labelling proteomics
    Article Snippet: .. An eGFP lentiviral control vector (VB010000-9389rbj; Vectorbuilder) was used throughout the study. ..

    Article Title: The Abca7 V1613M variant reduces Aβ generation, plaque load, and neuronal damage
    Article Snippet: .. An empty vector (contains EGFP only, Prp[Exp]‐EGFP/neo‐CAG > ORF_stuffer), mouse WT ABCA7 (Prp[Exp]‐EGFP/neo‐CAG > NM_001346081.2), and V1613M ABCA7 (Prp[Exp]‐EGFP/neo‐CAG > NM_001346081.2*V1613M) were designed and cloned using VectorBuilder (Chicago, IL). .. Transformed E. coli glycerol stocks were propagated, and plasmids purified using Monarch plasmid miniprep kit (T1010S, New England Biolabs, Ipswich, MA).

    Article Title: Sestrin2 ameliorates age-related spontaneous benign prostatic hyperplasia via activation of AMPK/mTOR dependent autophagy.
    Article Snippet: therapeutic role in spontaneous BPH.. In vivo study, the 3-month-old (3 M) and 24-month-old (24 M) mice were used, and the 24 M mice were additionally administered recombinant Sestrin2 protein (rp-Sestrin2) for consecutive 14 days.. In vitro, BPH-1 cells were transfected with an empty or Sestrin2 overexpression vector.

    Clone Assay:

    Article Title: The Abca7 V1613M variant reduces Aβ generation, plaque load, and neuronal damage
    Article Snippet: .. An empty vector (contains EGFP only, Prp[Exp]‐EGFP/neo‐CAG > ORF_stuffer), mouse WT ABCA7 (Prp[Exp]‐EGFP/neo‐CAG > NM_001346081.2), and V1613M ABCA7 (Prp[Exp]‐EGFP/neo‐CAG > NM_001346081.2*V1613M) were designed and cloned using VectorBuilder (Chicago, IL). .. Transformed E. coli glycerol stocks were propagated, and plasmids purified using Monarch plasmid miniprep kit (T1010S, New England Biolabs, Ipswich, MA).

    Transfection:

    Article Title: Sestrin2 ameliorates age-related spontaneous benign prostatic hyperplasia via activation of AMPK/mTOR dependent autophagy.
    Article Snippet: therapeutic role in spontaneous BPH.. In vivo study, the 3-month-old (3 M) and 24-month-old (24 M) mice were used, and the 24 M mice were additionally administered recombinant Sestrin2 protein (rp-Sestrin2) for consecutive 14 days.. In vitro, BPH-1 cells were transfected with an empty or Sestrin2 overexpression vector.

    Gene Expression:

    Article Title: Sestrin2 ameliorates age-related spontaneous benign prostatic hyperplasia via activation of AMPK/mTOR dependent autophagy.
    Article Snippet: therapeutic role in spontaneous BPH.. In vivo study, the 3-month-old (3 M) and 24-month-old (24 M) mice were used, and the 24 M mice were additionally administered recombinant Sestrin2 protein (rp-Sestrin2) for consecutive 14 days.. In vitro, BPH-1 cells were transfected with an empty or Sestrin2 overexpression vector.

    Expressing:

    Article Title: Sestrin2 ameliorates age-related spontaneous benign prostatic hyperplasia via activation of AMPK/mTOR dependent autophagy.
    Article Snippet: therapeutic role in spontaneous BPH.. In vivo study, the 3-month-old (3 M) and 24-month-old (24 M) mice were used, and the 24 M mice were additionally administered recombinant Sestrin2 protein (rp-Sestrin2) for consecutive 14 days.. In vitro, BPH-1 cells were transfected with an empty or Sestrin2 overexpression vector.



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    90
    VectorBuilder GmbH egfp lentiviral control vector
    a, HCT116 cells stably expressing BRIP1 R162Q were <t>transiently</t> <t>transfected</t> using PEI with empty vector <t>(EV-EGFP)</t> or RNaseH1-WT-eGFP. Transfection efficiency was assessed via EGFP fluorescence. b, immunoblot analysis confirming expression of GFP-tagged constructs in transfected cells. c, Immunofluorescence detection of R-loops using the S9.6 antibody in cells transfected with EGFP or EGFP-RNaseH1. d, Quantification of S9.6 mean fluorescence intensity (MFI) per nucleus. Data represent mean ± SEM of three independent experiments. e, Immunofluorescence detection of G-quadruplex (G4) structures using the BG4 antibody in cells transfected with EGFP or EGFP-RNaseH1. f, Quantification of BG4 fluorescence intensity per nucleus. Data represent mean ± SEM of three independent experiments. g, Representative DNA fiber assay images from HCT116 cells harboring BRIP1 R162Q transfected with empty vector (EV-eGFP) or RNaseH1-WT. DNA replication tracts were sequentially labeled with CldU (red) and IdU (green). h, Quantification of DNA fiber types. RNaseH1-WT expression reduced the proportion of stalled replication forks in cells harboring BRIP1 R162Q. i, While the overall fork speed was not significantly increased upon RNaseH1-WT expression, tracts displayed a more balanced distribution of CldU and IdU incorporation. j, Fork asymmetry analysis. RNaseH1-WT expression reduced fork asymmetry, indicating that left- and right-moving sister forks progressed at more similar speeds. Labeled tracks were visualized by confocal microscopy, and tract lengths were measured on raw LSM images. Lengths (µm) were converted to replication speed (kb/min) using a factor of 2.59 kb/µm. All data represent mean ± SEM from three independent experiments. At least 150 fibers per condition were analyzed in each replicate. Statistical significance was determined using paired two-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). c, e, Representative confocal images are shown.
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    a, HCT116 cells stably expressing BRIP1 R162Q were transiently transfected using PEI with empty vector (EV-EGFP) or RNaseH1-WT-eGFP. Transfection efficiency was assessed via EGFP fluorescence. b, immunoblot analysis confirming expression of GFP-tagged constructs in transfected cells. c, Immunofluorescence detection of R-loops using the S9.6 antibody in cells transfected with EGFP or EGFP-RNaseH1. d, Quantification of S9.6 mean fluorescence intensity (MFI) per nucleus. Data represent mean ± SEM of three independent experiments. e, Immunofluorescence detection of G-quadruplex (G4) structures using the BG4 antibody in cells transfected with EGFP or EGFP-RNaseH1. f, Quantification of BG4 fluorescence intensity per nucleus. Data represent mean ± SEM of three independent experiments. g, Representative DNA fiber assay images from HCT116 cells harboring BRIP1 R162Q transfected with empty vector (EV-eGFP) or RNaseH1-WT. DNA replication tracts were sequentially labeled with CldU (red) and IdU (green). h, Quantification of DNA fiber types. RNaseH1-WT expression reduced the proportion of stalled replication forks in cells harboring BRIP1 R162Q. i, While the overall fork speed was not significantly increased upon RNaseH1-WT expression, tracts displayed a more balanced distribution of CldU and IdU incorporation. j, Fork asymmetry analysis. RNaseH1-WT expression reduced fork asymmetry, indicating that left- and right-moving sister forks progressed at more similar speeds. Labeled tracks were visualized by confocal microscopy, and tract lengths were measured on raw LSM images. Lengths (µm) were converted to replication speed (kb/min) using a factor of 2.59 kb/µm. All data represent mean ± SEM from three independent experiments. At least 150 fibers per condition were analyzed in each replicate. Statistical significance was determined using paired two-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). c, e, Representative confocal images are shown.

    Journal: bioRxiv

    Article Title: Role of a childhood cancer-linked BRIP1/FANCJ germline variant in genomic instability and cancer cell vulnerability

    doi: 10.64898/2026.03.24.714005

    Figure Lengend Snippet: a, HCT116 cells stably expressing BRIP1 R162Q were transiently transfected using PEI with empty vector (EV-EGFP) or RNaseH1-WT-eGFP. Transfection efficiency was assessed via EGFP fluorescence. b, immunoblot analysis confirming expression of GFP-tagged constructs in transfected cells. c, Immunofluorescence detection of R-loops using the S9.6 antibody in cells transfected with EGFP or EGFP-RNaseH1. d, Quantification of S9.6 mean fluorescence intensity (MFI) per nucleus. Data represent mean ± SEM of three independent experiments. e, Immunofluorescence detection of G-quadruplex (G4) structures using the BG4 antibody in cells transfected with EGFP or EGFP-RNaseH1. f, Quantification of BG4 fluorescence intensity per nucleus. Data represent mean ± SEM of three independent experiments. g, Representative DNA fiber assay images from HCT116 cells harboring BRIP1 R162Q transfected with empty vector (EV-eGFP) or RNaseH1-WT. DNA replication tracts were sequentially labeled with CldU (red) and IdU (green). h, Quantification of DNA fiber types. RNaseH1-WT expression reduced the proportion of stalled replication forks in cells harboring BRIP1 R162Q. i, While the overall fork speed was not significantly increased upon RNaseH1-WT expression, tracts displayed a more balanced distribution of CldU and IdU incorporation. j, Fork asymmetry analysis. RNaseH1-WT expression reduced fork asymmetry, indicating that left- and right-moving sister forks progressed at more similar speeds. Labeled tracks were visualized by confocal microscopy, and tract lengths were measured on raw LSM images. Lengths (µm) were converted to replication speed (kb/min) using a factor of 2.59 kb/µm. All data represent mean ± SEM from three independent experiments. At least 150 fibers per condition were analyzed in each replicate. Statistical significance was determined using paired two-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). c, e, Representative confocal images are shown.

    Article Snippet: For R-loop removal, cells were transiently transfected with an EGFP empty vector control (pEGFP-C1, Takara Bio, #632470), EGFP-tagged RNaseH1 wild-type (WT), or the catalytically inactive RNaseH1 D145N mutant as indicated.

    Techniques: Stable Transfection, Expressing, Transfection, Plasmid Preparation, Fluorescence, Western Blot, Construct, Immunofluorescence, Labeling, Confocal Microscopy, Two Tailed Test