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widefield microscope  (Nikon)


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    Structured Review

    Nikon widefield microscope
    Widefield Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 12150 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+ti2+widefield+microscope/ECLIPSE+Ti2/pm42827176-339-3-6
    Average 99 stars, based on 12150 article reviews
    widefield microscope - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    Widefield:

    Article Title: Epstein Barr virus antigen-induced autoantibodies against complement C1q exacerbate renal disease in lupus-prone mice
    Article Snippet: For Gomori’s methenamine silver (sFOG) staining, sections were incubated in preheated methenamine silver solution (Bio-Optica, 04-130802) at 60 ;°C for 20 ;min, toned with 0.1% gold chloride (Electron Microscopy Sciences), fixed in 5% sodium thiosulfate (Merck), and counterstained with light Green SF Yellowish (Sigma-Aldrich, USA). .. Slides were dehydrated, cleared, and mounted in Eukitt mounting medium (Sigma-Aldrich, USA), and imaged using a Nikon Eclipse Ti2 widefield microscope with Plan Apo 20× and 40× objectives. .. Mouse kidneys were harvested and rinsed in ice-cold PBS (Thermo Fisher) to remove blood, snap-frozen in liquid nitrogen (Air Liquide, Zürich), and stored at −80 °C.

    Article Title: The CUP pilus SMF-1 utilizes a specific antiparallel bundling mechanism to initiate biofilm formation.
    Article Snippet: .. Images were taken on a Nikon Eclipse Ti2 widefield microscope (Nikon). ..

    Microscopy:

    Article Title: Epstein Barr virus antigen-induced autoantibodies against complement C1q exacerbate renal disease in lupus-prone mice
    Article Snippet: For Gomori’s methenamine silver (sFOG) staining, sections were incubated in preheated methenamine silver solution (Bio-Optica, 04-130802) at 60 ;°C for 20 ;min, toned with 0.1% gold chloride (Electron Microscopy Sciences), fixed in 5% sodium thiosulfate (Merck), and counterstained with light Green SF Yellowish (Sigma-Aldrich, USA). .. Slides were dehydrated, cleared, and mounted in Eukitt mounting medium (Sigma-Aldrich, USA), and imaged using a Nikon Eclipse Ti2 widefield microscope with Plan Apo 20× and 40× objectives. .. Mouse kidneys were harvested and rinsed in ice-cold PBS (Thermo Fisher) to remove blood, snap-frozen in liquid nitrogen (Air Liquide, Zürich), and stored at −80 °C.

    Article Title: The CUP pilus SMF-1 utilizes a specific antiparallel bundling mechanism to initiate biofilm formation.
    Article Snippet: .. Images were taken on a Nikon Eclipse Ti2 widefield microscope (Nikon). ..

    Article Title: QUILPEN Provides Independent and Label‐Free Single‐Cell Quantification of Pigmentation Dynamics and Organelle Content
    Article Snippet: Cells were counterstained with Hoechst 33342 (10 μg/mL; ThermoFisher, 62249) and mounted with Prolong Glass (# P36984 , Invitrogen). .. Cells were imaged on a Nikon Eclipse Ti2 Widefield microscope equipped with a X‐cite Xylis (XT720S) light source and imaged using a 0.45 NA CF160 Super Plan Fluor PH1 ADM ELWD 20× objective. .. Images for mCherry (Ex: 560/40 nm, Em: 635/60 nm), Cy5 (Ex: 620/50, Em 690/50) and nuclear Hoescht (Ex: 375/28 nm, Em: 435–485 nm) were captured with a sCMOS pco.panda camera and processed in ImageJ‐FIJI (Schindelin et al. ).

    Article Title: Obscurin A localizes near the cell membrane to modulate stress fiber dynamics and cell migration.
    Article Snippet: Cells at ~40% confluency were stained using Orange Cytopainter (Abcam; Cambridge UK) and allowed to sit in a solution of FluoroBrite medium (Thermo; Waltham MA) with Trolox. .. Plates were imaged using the Nikon Eclipse Ti2 widefield microscope and analyzed in ImageJ using the Trainable WEKA Segmentation and Mtrack2 plugins as previously described. (44) A power analysis indicated a required minimum of 23 cells per treatment (Supplemental Figure 3). ..

    Article Title: The archaic CUP pilus SMF-1 utilizes a specific antiparallel bundling mechanism to initiate biofilm formation
    Article Snippet: .. Images were taken on a Nikon Eclipse Ti2 widefield microscope (Nikon). ..

    Article Title: QUILPEN Provides Independent and Label‐Free Single‐Cell Quantification of Pigmentation Dynamics and Organelle Content
    Article Snippet: Images for mCherry (Ex: 560/40 nm, Em: 635/60 nm), Cy5 (Ex: 620/50, Em 690/50) and nuclear Hoescht (Ex: 375/28 nm, Em: 435–485 nm) were captured with a sCMOS pco.panda camera and processed in ImageJ‐FIJI (Schindelin et al. ). .. To stain melanin, cells were processed using Fontana‐Masson Stain Kit (#ab150669; Abcam), following the manufacturer's protocol and imaged on the Nikon Eclipse Ti2 Widefield microscope using a NA 0.45 CF160 Plan Fluor PH1 DLL 10× objective with the phase condenser open in the brightfield configuration. ..

    Article Title: Spatially correlated fluctuations govern relative chromatin motion
    Article Snippet: .. Cells were imaged in oblique illumination mode on a Nikon Eclipse Ti2 widefield microscope at 0.5 Hz (2 s frame interval) for 5 min, acquiring 9 z-planes spaced 0.3 μm apart. ..

    Article Title: Mitochondrial Transplantation Increases Bioenergetics and Neurite Outgrowth in Healthy and P301Ltau-Expressing SH-SY5Y Cells
    Article Snippet: .. Pictures were captured using an Eclipse Ti2 widefield microscope (Nikon). ..

    Staining:

    Article Title: QUILPEN Provides Independent and Label‐Free Single‐Cell Quantification of Pigmentation Dynamics and Organelle Content
    Article Snippet: Images for mCherry (Ex: 560/40 nm, Em: 635/60 nm), Cy5 (Ex: 620/50, Em 690/50) and nuclear Hoescht (Ex: 375/28 nm, Em: 435–485 nm) were captured with a sCMOS pco.panda camera and processed in ImageJ‐FIJI (Schindelin et al. ). .. To stain melanin, cells were processed using Fontana‐Masson Stain Kit (#ab150669; Abcam), following the manufacturer's protocol and imaged on the Nikon Eclipse Ti2 Widefield microscope using a NA 0.45 CF160 Plan Fluor PH1 DLL 10× objective with the phase condenser open in the brightfield configuration. ..



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    Nikon ti2 eclipse widefield microscope
    ( A ) Representative brain sections from 5XFAD and non-transgenic (non-TG) mice transduced with Nrf2+GFP or GFP-only AAVs and stained with DAPI (blue), BACE1 (Green) and Thiazine Red (ThR, red) to mark plaques and imaged by widefield immunofluorescence microscopy. Size bar = 500μm ( B ) Percent area covered by BACE immunostaining in 5XFAD cortex is elevated, which has a non-significant downward trend (p=0.07) caused by neuronal Nrf2 overexpression ( C ) Percent area covered by BACE immunostaining in 5XFAD hippocampus is elevated, which is significantly reduced by neuronal Nrf2 overexpression. 2-way ANOVA with Sidak’s multiple comparisons test * p<0.05, ** p<0.01, **** p<0.0001 ( D ) Representative single plane confocal images of amyloid plaques (red, Thiazine red, *= plaque core) surrounded by dystrophic neurites immunostained for BACE1, LAMP1, and p-tau181 (left to right, green) in GFP-only or Nrf2+GFP AAV transduced 5XFAD mice. Note that in the right panel of images from Nrf2 overexpressing mice, dystrophic neurites are smaller, fewer and of less overall plaque coverage than upper panel (arrows). Size bar = 10μm. ( E-G ) Quantification of the ratio of dystrophic neurite proteins (BACE, E; LAMP1, F; tau p181, G) to amyloid plaque core (Thiazine red, E, G; Aβ42, F) in cortex of GFP-only or Nrf2+GFP AAV transduced 5XFAD mice. In all cases, the ratios are reduced in neuronal Nrf2 overexpressing 5XFAD mice, indicating reduced dystrophic neurites for a given amount of amyloid. ( H-J ) Quantification of the mean areas of BACE1, LAMP1 or tau p181 immuno-positive structures around individual amyloid plaques in cortex. For all three proteins, the average areas of immunostaining are reduced in Nrf2 overexpressing mice. ( E-J ) quantification from 10x <t>Ti2</t> widefield images, using NIS Elements, as described in methods. * p<0.05, ** p<0.01, two tailed t-test. NB: Images are false colored for viewing. Imaging channels were as follows: DAPI, NeuN – 405nm, ThR, Aβ42 – 568nm, BACE1, Aβ42, p181 - 647nm. Non-TG GFP n= 5 (3F,3M), non-TG Nrf2+GFP n= 6 (4F, 2M), 5XFAD GFP n= 10 (5F, 5M), 5XFAD GFP+Nrf2 n= 10 (5F, 5M)
    Ti2 Eclipse Widefield Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    ( A ) Representative brain sections from 5XFAD and non-transgenic (non-TG) mice transduced with Nrf2+GFP or GFP-only AAVs and stained with DAPI (blue), BACE1 (Green) and Thiazine Red (ThR, red) to mark plaques and imaged by widefield immunofluorescence microscopy. Size bar = 500μm ( B ) Percent area covered by BACE immunostaining in 5XFAD cortex is elevated, which has a non-significant downward trend (p=0.07) caused by neuronal Nrf2 overexpression ( C ) Percent area covered by BACE immunostaining in 5XFAD hippocampus is elevated, which is significantly reduced by neuronal Nrf2 overexpression. 2-way ANOVA with Sidak’s multiple comparisons test * p<0.05, ** p<0.01, **** p<0.0001 ( D ) Representative single plane confocal images of amyloid plaques (red, Thiazine red, *= plaque core) surrounded by dystrophic neurites immunostained for BACE1, LAMP1, and p-tau181 (left to right, green) in GFP-only or Nrf2+GFP AAV transduced 5XFAD mice. Note that in the right panel of images from Nrf2 overexpressing mice, dystrophic neurites are smaller, fewer and of less overall plaque coverage than upper panel (arrows). Size bar = 10μm. ( E-G ) Quantification of the ratio of dystrophic neurite proteins (BACE, E; LAMP1, F; tau p181, G) to amyloid plaque core (Thiazine red, E, G; Aβ42, F) in cortex of GFP-only or Nrf2+GFP AAV transduced 5XFAD mice. In all cases, the ratios are reduced in neuronal Nrf2 overexpressing 5XFAD mice, indicating reduced dystrophic neurites for a given amount of amyloid. ( H-J ) Quantification of the mean areas of BACE1, LAMP1 or tau p181 immuno-positive structures around individual amyloid plaques in cortex. For all three proteins, the average areas of immunostaining are reduced in Nrf2 overexpressing mice. ( E-J ) quantification from 10x Ti2 widefield images, using NIS Elements, as described in methods. * p<0.05, ** p<0.01, two tailed t-test. NB: Images are false colored for viewing. Imaging channels were as follows: DAPI, NeuN – 405nm, ThR, Aβ42 – 568nm, BACE1, Aβ42, p181 - 647nm. Non-TG GFP n= 5 (3F,3M), non-TG Nrf2+GFP n= 6 (4F, 2M), 5XFAD GFP n= 10 (5F, 5M), 5XFAD GFP+Nrf2 n= 10 (5F, 5M)

    Journal: bioRxiv

    Article Title: Neuronal overexpression of Nrf2 reduces dystrophic neurites in 5XFAD Alzheimer’s disease model mice

    doi: 10.64898/2026.03.16.711596

    Figure Lengend Snippet: ( A ) Representative brain sections from 5XFAD and non-transgenic (non-TG) mice transduced with Nrf2+GFP or GFP-only AAVs and stained with DAPI (blue), BACE1 (Green) and Thiazine Red (ThR, red) to mark plaques and imaged by widefield immunofluorescence microscopy. Size bar = 500μm ( B ) Percent area covered by BACE immunostaining in 5XFAD cortex is elevated, which has a non-significant downward trend (p=0.07) caused by neuronal Nrf2 overexpression ( C ) Percent area covered by BACE immunostaining in 5XFAD hippocampus is elevated, which is significantly reduced by neuronal Nrf2 overexpression. 2-way ANOVA with Sidak’s multiple comparisons test * p<0.05, ** p<0.01, **** p<0.0001 ( D ) Representative single plane confocal images of amyloid plaques (red, Thiazine red, *= plaque core) surrounded by dystrophic neurites immunostained for BACE1, LAMP1, and p-tau181 (left to right, green) in GFP-only or Nrf2+GFP AAV transduced 5XFAD mice. Note that in the right panel of images from Nrf2 overexpressing mice, dystrophic neurites are smaller, fewer and of less overall plaque coverage than upper panel (arrows). Size bar = 10μm. ( E-G ) Quantification of the ratio of dystrophic neurite proteins (BACE, E; LAMP1, F; tau p181, G) to amyloid plaque core (Thiazine red, E, G; Aβ42, F) in cortex of GFP-only or Nrf2+GFP AAV transduced 5XFAD mice. In all cases, the ratios are reduced in neuronal Nrf2 overexpressing 5XFAD mice, indicating reduced dystrophic neurites for a given amount of amyloid. ( H-J ) Quantification of the mean areas of BACE1, LAMP1 or tau p181 immuno-positive structures around individual amyloid plaques in cortex. For all three proteins, the average areas of immunostaining are reduced in Nrf2 overexpressing mice. ( E-J ) quantification from 10x Ti2 widefield images, using NIS Elements, as described in methods. * p<0.05, ** p<0.01, two tailed t-test. NB: Images are false colored for viewing. Imaging channels were as follows: DAPI, NeuN – 405nm, ThR, Aβ42 – 568nm, BACE1, Aβ42, p181 - 647nm. Non-TG GFP n= 5 (3F,3M), non-TG Nrf2+GFP n= 6 (4F, 2M), 5XFAD GFP n= 10 (5F, 5M), 5XFAD GFP+Nrf2 n= 10 (5F, 5M)

    Article Snippet: Images were acquired on a Nikon Ti2 Eclipse widefield microscope with a 10x objective, using the NIS Elements software high content method to capture and tile whole sections.

    Techniques: Transgenic Assay, Transduction, Staining, Immunofluorescence, Microscopy, Immunostaining, Over Expression, Two Tailed Test, Imaging