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eclipse te300 inverted fluorescence phase contrast microscope  (Nikon)


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    Structured Review

    Nikon eclipse te300 inverted fluorescence phase contrast microscope
    Fluorescence of spirochetes transformed with a GFP plasmid. B. afzelii #1192 transformed with in vitro methylated (a) and with unmethylated pBSV2_OspA_GFP (b). B. afzelii #1201 transformed with in vitro methylated (c) and with unmethylated pBSV2_OspA_GFP (d). B. garinii #1195 transformed with in vitro methylated pBSV2_OspA_GFP (e). B. garinii #1226 transformed with in vitro methylated pBSV2_OspA_GFP (f). All cultures were grown in modified BSK II medium at 34°C. All photos were taken using a Nikon Eclipse <t>TE300</t> Inverted Fluorescence Phase Contrast Microscope using a 40x objective.
    Eclipse Te300 Inverted Fluorescence Phase Contrast Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 59597 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+te300+inverted+microscope/Objectives/pmc12811465-194-7-6
    Average 99 stars, based on 59597 article reviews
    eclipse te300 inverted fluorescence phase contrast microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Comparative analysis of Borrelia’s Defence mechanisms and their impact on genetic manipulation of low-passage isolates of Borrelia afzelii and Borrelia garinii"

    Article Title: Comparative analysis of Borrelia’s Defence mechanisms and their impact on genetic manipulation of low-passage isolates of Borrelia afzelii and Borrelia garinii

    Journal: Current Research in Microbial Sciences

    doi: 10.1016/j.crmicr.2025.100543

    Fluorescence of spirochetes transformed with a GFP plasmid. B. afzelii #1192 transformed with in vitro methylated (a) and with unmethylated pBSV2_OspA_GFP (b). B. afzelii #1201 transformed with in vitro methylated (c) and with unmethylated pBSV2_OspA_GFP (d). B. garinii #1195 transformed with in vitro methylated pBSV2_OspA_GFP (e). B. garinii #1226 transformed with in vitro methylated pBSV2_OspA_GFP (f). All cultures were grown in modified BSK II medium at 34°C. All photos were taken using a Nikon Eclipse TE300 Inverted Fluorescence Phase Contrast Microscope using a 40x objective.
    Figure Legend Snippet: Fluorescence of spirochetes transformed with a GFP plasmid. B. afzelii #1192 transformed with in vitro methylated (a) and with unmethylated pBSV2_OspA_GFP (b). B. afzelii #1201 transformed with in vitro methylated (c) and with unmethylated pBSV2_OspA_GFP (d). B. garinii #1195 transformed with in vitro methylated pBSV2_OspA_GFP (e). B. garinii #1226 transformed with in vitro methylated pBSV2_OspA_GFP (f). All cultures were grown in modified BSK II medium at 34°C. All photos were taken using a Nikon Eclipse TE300 Inverted Fluorescence Phase Contrast Microscope using a 40x objective.

    Techniques Used: Fluorescence, Transformation Assay, Plasmid Preparation, In Vitro, Methylation, Modification, Microscopy

    Related Articles

    Inverted Microscopy:

    Article Title: Exosomes are specialized vehicles to induce fibronectin assembly
    Article Snippet: Tissues underwent the antigen retrieval step again for 20 min if the same tissue would be subjected to multiple labeling before being mounted in ProLong Gold with DAPI (Thermo Fisher Scientific P36931). .. Images were collected using a Nikon Eclipse TE300 inverted microscope with a Nikon Plan Fluor ELWD 20X/0.45 DIC L objective lens and an ORCA-R2 Digital CCD camera. ..

    Article Title: The T3SS structural and effector genes of Chlamydia trachomatis are expressed in distinct phenotypic cell forms
    Article Snippet: .. Inclusions were imaged using a Nikon Eclipse TE300 inverted microscope utilizing a scopeLED lamp at 470 and 390 nm and BrightLine band pass emissions filters at 514/30 nm and 434/17 nm. ..

    Article Title: Discovery of a bell-shaped dose response curve to melanin-concentrating hormone in the 3T3-L1 adipocyte model: low-dose MCH facilitates adipogenesis
    Article Snippet: .. Phase contrast images were obtained soon after cell staining to reduce the loss of morphological detail using a Nikon Eclipse TE300 inverted microscope with a 40X objective and INFINITY3 Capture software. .. For quantification of lipid droplets, images of fifty randomly chosen adipocytes were captured, circles were drawn around each individual lipid droplet within the cell, and the number of circles within each cell were counted using the ‘analyze particles’ function of NIH ImageJ and exported to an MS Excel file.

    Article Title: Echs1 -mediated histone crotonylation facilitates zygotic genome activation and expression of repetitive elements in early mammalian embryos
    Article Snippet: .. Using Nikon Eclipse TE300 Inverted Microscope (Nikon, Japan), 5-10 pl of siRNA was injected into the cytoplasm of GV oocytes. ..

    Article Title: M2-like macrophages derived from THP-1 cells promote myofibroblast differentiation of synovial fibroblasts in association with the TGF-β1/SMAD2/3 signaling pathway.
    Article Snippet: .. Phase contrast images of cells were captured using an Eclipse TE300 inverted microscope (Nikon, Tokyo, Japan). .. For the confirmation of differentiation by real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR), total RNA was extracted using a PureLink RNA Mini Kit (Thermo Fisher Scientific, Scientific Reports | (2025) 15:25505 2| https://doi.org/10.1038/s41598-025-10858-6 Waltham, MA, USA). cDNA synthesis was performed using a High-Capacity RNA-to-cDNA Kit (Thermo Fisher Scientific, Waltham, MA, USA) with a GeneAmp PCR System 9700 thermal cycler (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: A positive allosteric modulator of the β 1 AR with antagonist activity for catecholaminergic polymorphic ventricular tachycardia
    Article Snippet: .. After pretreatment, cells were stimulated with isoproterenol (0.01–1 μM) or left untreated (basal condition) and plated immediately in an FHD rotational cell chamber (Ionoptix) mounted on a Nikon Eclipse TE300 inverted microscope (40× 0.9 NA objective, MRF00400, Nikon). .. Myocytes were paced at 1Hz (20 V, MyoPacer, Ionoptix), and sarcomere length was recorded with IonWizard 7.2 using the MyoCam-S camera (Ionoptix).

    Article Title: Cannabinoid Receptor 2 Activating Antibodies: A Promising Therapeutic Strategy for Macrophage-Driven Fibro-Inflammatory Diseases
    Article Snippet: .. A Nikon Eclipse TE300 Inverted Microscope equipped with a Hg short arc HBO lamp (OSRAM) was used to acquire images. ..

    Article Title: Comparison of Gold Nanoparticle and Fluorophore-conjugated Antibodies for Labeling Epitopes in Permeabilized Cells.
    Article Snippet: Immunohistochemistry (IHC) and immunofluorescence (IF) are longstanding diagnostic tools, offering crucial insights into the spatial distribution and expression patterns of biomolecules within tissues.1–3 Most commonly applied in immunostaining practices due to the high selectivity of antibodies to their respective antigen proteins, IHC and IF have many applications in diagnostic pathology and basic research due to the relative simplicity of the assay.4,5 These staining techniques can be run as a direct or indirect method, where the labeled antibodies are bound to the primary target or a primary antibody specific for the epitope of interest, respectively.6 As with any such test, false positives and cases of ambiguous staining occur.. Indirect methods lower false positives in IHC and IF assays through washing steps to remove unbound antibodies and multiple secondary antibodies bound to the primary, leading to higher signal to noise.6 1348502 JHCXXX10.1369/00221554251348502Nanoparticle Performance in ImmunocytochemistryBaugher et al. research-article2025

    Staining:

    Article Title: Discovery of a bell-shaped dose response curve to melanin-concentrating hormone in the 3T3-L1 adipocyte model: low-dose MCH facilitates adipogenesis
    Article Snippet: .. Phase contrast images were obtained soon after cell staining to reduce the loss of morphological detail using a Nikon Eclipse TE300 inverted microscope with a 40X objective and INFINITY3 Capture software. .. For quantification of lipid droplets, images of fifty randomly chosen adipocytes were captured, circles were drawn around each individual lipid droplet within the cell, and the number of circles within each cell were counted using the ‘analyze particles’ function of NIH ImageJ and exported to an MS Excel file.

    Software:

    Article Title: Discovery of a bell-shaped dose response curve to melanin-concentrating hormone in the 3T3-L1 adipocyte model: low-dose MCH facilitates adipogenesis
    Article Snippet: .. Phase contrast images were obtained soon after cell staining to reduce the loss of morphological detail using a Nikon Eclipse TE300 inverted microscope with a 40X objective and INFINITY3 Capture software. .. For quantification of lipid droplets, images of fifty randomly chosen adipocytes were captured, circles were drawn around each individual lipid droplet within the cell, and the number of circles within each cell were counted using the ‘analyze particles’ function of NIH ImageJ and exported to an MS Excel file.

    Article Title: Comparison of Gold Nanoparticle and Fluorophore-conjugated Antibodies for Labeling Epitopes in Permeabilized Cells.
    Article Snippet: Immunohistochemistry (IHC) and immunofluorescence (IF) are longstanding diagnostic tools, offering crucial insights into the spatial distribution and expression patterns of biomolecules within tissues.1–3 Most commonly applied in immunostaining practices due to the high selectivity of antibodies to their respective antigen proteins, IHC and IF have many applications in diagnostic pathology and basic research due to the relative simplicity of the assay.4,5 These staining techniques can be run as a direct or indirect method, where the labeled antibodies are bound to the primary target or a primary antibody specific for the epitope of interest, respectively.6 As with any such test, false positives and cases of ambiguous staining occur.. Indirect methods lower false positives in IHC and IF assays through washing steps to remove unbound antibodies and multiple secondary antibodies bound to the primary, leading to higher signal to noise.6 1348502 JHCXXX10.1369/00221554251348502Nanoparticle Performance in ImmunocytochemistryBaugher et al. research-article2025

    Injection:

    Article Title: Echs1 -mediated histone crotonylation facilitates zygotic genome activation and expression of repetitive elements in early mammalian embryos
    Article Snippet: .. Using Nikon Eclipse TE300 Inverted Microscope (Nikon, Japan), 5-10 pl of siRNA was injected into the cytoplasm of GV oocytes. ..



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    Fluorescence of spirochetes transformed with a GFP plasmid. B. afzelii #1192 transformed with in vitro methylated (a) and with unmethylated pBSV2_OspA_GFP (b). B. afzelii #1201 transformed with in vitro methylated (c) and with unmethylated pBSV2_OspA_GFP (d). B. garinii #1195 transformed with in vitro methylated pBSV2_OspA_GFP (e). B. garinii #1226 transformed with in vitro methylated pBSV2_OspA_GFP (f). All cultures were grown in modified BSK II medium at 34°C. All photos were taken using a Nikon Eclipse <t>TE300</t> Inverted Fluorescence Phase Contrast Microscope using a 40x objective.
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    Fluorescence of spirochetes transformed with a GFP plasmid. B. afzelii #1192 transformed with in vitro methylated (a) and with unmethylated pBSV2_OspA_GFP (b). B. afzelii #1201 transformed with in vitro methylated (c) and with unmethylated pBSV2_OspA_GFP (d). B. garinii #1195 transformed with in vitro methylated pBSV2_OspA_GFP (e). B. garinii #1226 transformed with in vitro methylated pBSV2_OspA_GFP (f). All cultures were grown in modified BSK II medium at 34°C. All photos were taken using a Nikon Eclipse <t>TE300</t> Inverted Fluorescence Phase Contrast Microscope using a 40x objective.
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    Fluorescence of spirochetes transformed with a GFP plasmid. B. afzelii #1192 transformed with in vitro methylated (a) and with unmethylated pBSV2_OspA_GFP (b). B. afzelii #1201 transformed with in vitro methylated (c) and with unmethylated pBSV2_OspA_GFP (d). B. garinii #1195 transformed with in vitro methylated pBSV2_OspA_GFP (e). B. garinii #1226 transformed with in vitro methylated pBSV2_OspA_GFP (f). All cultures were grown in modified BSK II medium at 34°C. All photos were taken using a Nikon Eclipse <t>TE300</t> Inverted Fluorescence Phase Contrast Microscope using a 40x objective.
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    Fluorescence of spirochetes transformed with a GFP plasmid. B. afzelii #1192 transformed with in vitro methylated (a) and with unmethylated pBSV2_OspA_GFP (b). B. afzelii #1201 transformed with in vitro methylated (c) and with unmethylated pBSV2_OspA_GFP (d). B. garinii #1195 transformed with in vitro methylated pBSV2_OspA_GFP (e). B. garinii #1226 transformed with in vitro methylated pBSV2_OspA_GFP (f). All cultures were grown in modified BSK II medium at 34°C. All photos were taken using a Nikon Eclipse <t>TE300</t> Inverted Fluorescence Phase Contrast Microscope using a 40x objective.
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    Image Search Results


    Fluorescence of spirochetes transformed with a GFP plasmid. B. afzelii #1192 transformed with in vitro methylated (a) and with unmethylated pBSV2_OspA_GFP (b). B. afzelii #1201 transformed with in vitro methylated (c) and with unmethylated pBSV2_OspA_GFP (d). B. garinii #1195 transformed with in vitro methylated pBSV2_OspA_GFP (e). B. garinii #1226 transformed with in vitro methylated pBSV2_OspA_GFP (f). All cultures were grown in modified BSK II medium at 34°C. All photos were taken using a Nikon Eclipse TE300 Inverted Fluorescence Phase Contrast Microscope using a 40x objective.

    Journal: Current Research in Microbial Sciences

    Article Title: Comparative analysis of Borrelia’s Defence mechanisms and their impact on genetic manipulation of low-passage isolates of Borrelia afzelii and Borrelia garinii

    doi: 10.1016/j.crmicr.2025.100543

    Figure Lengend Snippet: Fluorescence of spirochetes transformed with a GFP plasmid. B. afzelii #1192 transformed with in vitro methylated (a) and with unmethylated pBSV2_OspA_GFP (b). B. afzelii #1201 transformed with in vitro methylated (c) and with unmethylated pBSV2_OspA_GFP (d). B. garinii #1195 transformed with in vitro methylated pBSV2_OspA_GFP (e). B. garinii #1226 transformed with in vitro methylated pBSV2_OspA_GFP (f). All cultures were grown in modified BSK II medium at 34°C. All photos were taken using a Nikon Eclipse TE300 Inverted Fluorescence Phase Contrast Microscope using a 40x objective.

    Article Snippet: All photos were taken using a Nikon Eclipse TE300 Inverted Fluorescence Phase Contrast Microscope using a 40x objective.

    Techniques: Fluorescence, Transformation Assay, Plasmid Preparation, In Vitro, Methylation, Modification, Microscopy