z theta 16 well array station  (Applied BioPhysics)

Journal: PLoS ONE

Article Title: Correlation of host inflammatory cytokines and immune-related metabolites, but not viral NS1 protein, with disease severity of dengue virus infection

doi: 10.1371/journal.pone.0237141

Figure Lengend Snippet: The number of samples tested positive for DENV PCR, NS1 ELISA and IgM ELISA among the 10 serum samples selected for each dengue category accompanied by the day of illness. These ten samples consisted of five previously selected samples from ECIS measurement and five serum samples randomly selected from a pool of dengue positive samples, as well as for the healthy individuals.

Article Snippet: The real time changes in impedance (resistance and capacitance) induced in the MECs after treatment was detected using multiple alternative current (AC) frequencies in an ECIS Zθ 16-well array station (Applied Biophysics) for 72 hours.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: PLoS Pathogens

Article Title: Endothelial Cell Permeability during Hantavirus Infection Involves Factor XII-Dependent Increased Activation of the Kallikrein-Kinin System

doi: 10.1371/journal.ppat.1003470

Figure Lengend Snippet: Mock-, HTNV-, or ANDV-infected HUVEC were trypsinized, seeded onto ECIS chamberslides, and cultured until confluent. Media were removed and replaced with phenol red free EBM containing Zn 2+ . After an equilibration, 0.2 ml of phenol red free EBM containing Zn 2+ , FXII, PK, and HK (100 nM each) were added to cells (time zero) (black lines) (A). To measure inhibition of activation, some samples were treated with CTI (1 µM) (blue line), PKSI-527 (5 µM) (red line), or HOE 140 (1 µM) (green line) simultaneously with factors (B, C, & D). Real time measurements frequency measurements were taken throughout the assay.

Article Snippet: Arrays were then loaded into the ECIS 16-well array station and stabilized using ECIS software v1.2.92.0 (Applied Biophysics).

Techniques: Infection, Cell Culture, Inhibition, Activation Assay