anti e2f1 (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology
anti e2f1
Anti E2f1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1672 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e2f/E2F-1+Antibody/pm42811076-732-173-174
Average 96 stars, based on 1672 article reviews
Anti E2f1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1672 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e2f/E2F-1+Antibody/pm42811076-732-173-174
Average 96 stars, based on 1672 article reviews
anti e2f1 - by Bioz Stars,
2026-10
96/100 stars
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Immunoprecipitation:Article Title: Anti-Atherosclerotic Effect of Hibiscus Leaf Polyphenols against Tumor Necrosis Factor-alpha-Induced Abnormal Vascular Smooth Muscle Cell Migration and Proliferation Article Snippet: .. In short, 500 μg of protein from cell lysates was precleared with protein A–agarose beads (Pierce Biotechnology, Rockford, IL, USA), followed by immunoprecipitation using polyclonal antibodies against cdk2 or Incubation:Article Title: Crude extract and solvent fractions of Calystegia soldanella induce G1 and S phase arrest of the cell cycle in HepG2 cells Article Snippet: The protein samples were separated by 12% SDS-PAGE, and transferred to a polyvinylidene difluoride membrane (Millipore Corp., MA, USA). .. The membranes were blocked by incubation with 1% bovine serum albumin (BSA) in Tris-buffered saline-Tween-20 [TBS-T; 10 mM Tris-HCl, 150 mM NaCl (pH 7.5) containing 0.1% Tween-20] at room temperature for 1 h and incubated for 3 h with primary antibodies against GAPDH, cyclin D, cyclin E, cyclin A, cyclin-dependent kinase (CDK)2, CDK4, CDK6, CDC25A, p21, p27, retinoblastoma (RB), and Article Title: YAP1 maintains active chromatin state in head and neck squamous cell carcinomas that promotes tumorigenesis through cooperation with BRD4. Article Snippet: .. After blocking, the blots were incubated overnight at 4 °C with different antibodies against β-actin (Sigma, USA), cyclin D1, CDK4, Rb, AMPK, GSK3β (Cell Signaling Technology, MA, USA), Article Title: CPT1A-mediated fatty acid oxidation promotes cell proliferation via nucleoside metabolism in nasopharyngeal carcinoma Article Snippet: .. After blocking, the blots were incubated overnight at 4 °C with different antibodies against β-actin (Sigma, USA), cyclin D1, CDK4, Rb, AMPK, GSK3β (Cell Signaling Technology, MA, USA), Article Title: Anti-Atherosclerotic Effect of Hibiscus Leaf Polyphenols against Tumor Necrosis Factor-alpha-Induced Abnormal Vascular Smooth Muscle Cell Migration and Proliferation Article Snippet: Equal amounts of proteins were separated by 8–15% SDS–polyacrylamide gels and transferred to nitrocellulose membranes (Bio-Rad Labs., Hercules, CA, USA). .. In order to block non-specific binding, the nitrocellulose membranes were incubated with 5% nonfat dry milk for 1–2 h at 4 °C, and then overnight with polyclonal first antibodies against MMP-2, MMP-9, p-Akt, Akt, p-ERK, ERK, c-Jun, c-Fos, NF-κB, p-p53, p53, p21, p27, p16, PCNA (proliferating cell nuclear antigen), Saline:Article Title: Crude extract and solvent fractions of Calystegia soldanella induce G1 and S phase arrest of the cell cycle in HepG2 cells Article Snippet: The protein samples were separated by 12% SDS-PAGE, and transferred to a polyvinylidene difluoride membrane (Millipore Corp., MA, USA). .. The membranes were blocked by incubation with 1% bovine serum albumin (BSA) in Tris-buffered saline-Tween-20 [TBS-T; 10 mM Tris-HCl, 150 mM NaCl (pH 7.5) containing 0.1% Tween-20] at room temperature for 1 h and incubated for 3 h with primary antibodies against GAPDH, cyclin D, cyclin E, cyclin A, cyclin-dependent kinase (CDK)2, CDK4, CDK6, CDC25A, p21, p27, retinoblastoma (RB), and Western Blot:Article Title: Targeted breast cancer therapy by harnessing the inherent blood group antigen immune system Article Snippet: .. The antibodies used in this study for western blot included anti-mouse β-Actin (1:2000, Abcam, UK), Cyclin D, Cyclin E, Cyclin B1 (1:1000,abcam,UK), CDK1, CDK2, Article Title: Inhibitor for use for preventing and/or treating an infection with hepatitis B virus Article Snippet: Images were acquired with the confocal microscope zeiss LSM 780 NLO. .. TABLE 7 Antibodies used for co-immunoprecipitation, ChiP and western blotting experiments Target Company Dilution HBc for PLA, co-IP and ChIP Invitrogen 4 μg Hira for PLA, ChIP and WB Abeam Ab20655 2.5 μg/1:500 H3.3 for ChIP Abeam Ab62642 5 μg RNA Polymerase II for ChIP Diagenode 1 μg RPA 70 for ChIP Abeam Ab79398 5 μg BrdU for ChIP BD Pharmingen 5 μg Blocking Assay:Article Title: YAP1 maintains active chromatin state in head and neck squamous cell carcinomas that promotes tumorigenesis through cooperation with BRD4. Article Snippet: .. After blocking, the blots were incubated overnight at 4 °C with different antibodies against β-actin (Sigma, USA), cyclin D1, CDK4, Rb, AMPK, GSK3β (Cell Signaling Technology, MA, USA), Article Title: CPT1A-mediated fatty acid oxidation promotes cell proliferation via nucleoside metabolism in nasopharyngeal carcinoma Article Snippet: .. After blocking, the blots were incubated overnight at 4 °C with different antibodies against β-actin (Sigma, USA), cyclin D1, CDK4, Rb, AMPK, GSK3β (Cell Signaling Technology, MA, USA), Article Title: Anti-Atherosclerotic Effect of Hibiscus Leaf Polyphenols against Tumor Necrosis Factor-alpha-Induced Abnormal Vascular Smooth Muscle Cell Migration and Proliferation Article Snippet: Equal amounts of proteins were separated by 8–15% SDS–polyacrylamide gels and transferred to nitrocellulose membranes (Bio-Rad Labs., Hercules, CA, USA). .. In order to block non-specific binding, the nitrocellulose membranes were incubated with 5% nonfat dry milk for 1–2 h at 4 °C, and then overnight with polyclonal first antibodies against MMP-2, MMP-9, p-Akt, Akt, p-ERK, ERK, c-Jun, c-Fos, NF-κB, p-p53, p53, p21, p27, p16, PCNA (proliferating cell nuclear antigen), Proximity Ligation Assay:Article Title: Inhibitor for use for preventing and/or treating an infection with hepatitis B virus Article Snippet: Images were acquired with the confocal microscope zeiss LSM 780 NLO. .. TABLE 7 Antibodies used for co-immunoprecipitation, ChiP and western blotting experiments Target Company Dilution HBc for PLA, co-IP and ChIP Invitrogen 4 μg Hira for PLA, ChIP and WB Abeam Ab20655 2.5 μg/1:500 H3.3 for ChIP Abeam Ab62642 5 μg RNA Polymerase II for ChIP Diagenode 1 μg RPA 70 for ChIP Abeam Ab79398 5 μg BrdU for ChIP BD Pharmingen 5 μg Chromatin Immunoprecipitation:Article Title: Inhibitor for use for preventing and/or treating an infection with hepatitis B virus Article Snippet: Images were acquired with the confocal microscope zeiss LSM 780 NLO. .. TABLE 7 Antibodies used for co-immunoprecipitation, ChiP and western blotting experiments Target Company Dilution HBc for PLA, co-IP and ChIP Invitrogen 4 μg Hira for PLA, ChIP and WB Abeam Ab20655 2.5 μg/1:500 H3.3 for ChIP Abeam Ab62642 5 μg RNA Polymerase II for ChIP Diagenode 1 μg RPA 70 for ChIP Abeam Ab79398 5 μg BrdU for ChIP BD Pharmingen 5 μg Recombinase Polymerase Amplification:Article Title: Inhibitor for use for preventing and/or treating an infection with hepatitis B virus Article Snippet: Images were acquired with the confocal microscope zeiss LSM 780 NLO. .. TABLE 7 Antibodies used for co-immunoprecipitation, ChiP and western blotting experiments Target Company Dilution HBc for PLA, co-IP and ChIP Invitrogen 4 μg Hira for PLA, ChIP and WB Abeam Ab20655 2.5 μg/1:500 H3.3 for ChIP Abeam Ab62642 5 μg RNA Polymerase II for ChIP Diagenode 1 μg RPA 70 for ChIP Abeam Ab79398 5 μg BrdU for ChIP BD Pharmingen 5 μg Binding Assay:Article Title: Anti-Atherosclerotic Effect of Hibiscus Leaf Polyphenols against Tumor Necrosis Factor-alpha-Induced Abnormal Vascular Smooth Muscle Cell Migration and Proliferation Article Snippet: Equal amounts of proteins were separated by 8–15% SDS–polyacrylamide gels and transferred to nitrocellulose membranes (Bio-Rad Labs., Hercules, CA, USA). .. In order to block non-specific binding, the nitrocellulose membranes were incubated with 5% nonfat dry milk for 1–2 h at 4 °C, and then overnight with polyclonal first antibodies against MMP-2, MMP-9, p-Akt, Akt, p-ERK, ERK, c-Jun, c-Fos, NF-κB, p-p53, p53, p21, p27, p16, PCNA (proliferating cell nuclear antigen), |
