e2 ube2l3 (TargetMol)
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e2 ube2l3
E2 Ube2l3, supplied by TargetMol, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e2+ube2l3/UBE2L6+Protein%2C+Human%2C+Recombinant/pmc12830633-52-44-45
Average 93 stars, based on 1 article reviews
E2 Ube2l3, supplied by TargetMol, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e2+ube2l3/UBE2L6+Protein%2C+Human%2C+Recombinant/pmc12830633-52-44-45
Average 93 stars, based on 1 article reviews
e2 ube2l3 - by Bioz Stars,
2026-09
93/100 stars
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Purification:Article Title: TRIM39 aggravates hepatocellular carcinoma growth through targeting the p62-KEAP1-NRF2 axis. Article Snippet: .. Briefly, Purified recombinant His-p62 and GST- 178 TRIM39 proteins were incubated in a mixture of ubiquitin ligation 179 reaction containing 5 mM MgCl2, 50 mM Tris-HCl (pH 7.5), 2 mM 180 DTT, 4 mM ATP, 1 μg ubiquitin (Yeasen, 20431ES08), 300 ng E1- 181 UBA1 (Targetmol, TMPY-02840), and 500 ng Article Title: TRIM39 aggravates hepatocellular carcinoma growth through targeting the p62-KEAP1-NRF2 axis Article Snippet: .. Briefly, Purified recombinant His-p62 and GST-TRIM39 proteins were incubated in a mixture of ubiquitin ligation reaction containing 5 mM MgCl2, 50 mM Tris-HCl (pH 7.5), 2 mM DTT, 4 mM ATP, 1 μg ubiquitin (Yeasen, 20431ES08), 300 ng E1-UBA1 (Targetmol, TMPY-02840), and 500 ng Recombinant:Article Title: TRIM39 aggravates hepatocellular carcinoma growth through targeting the p62-KEAP1-NRF2 axis. Article Snippet: .. Briefly, Purified recombinant His-p62 and GST- 178 TRIM39 proteins were incubated in a mixture of ubiquitin ligation 179 reaction containing 5 mM MgCl2, 50 mM Tris-HCl (pH 7.5), 2 mM 180 DTT, 4 mM ATP, 1 μg ubiquitin (Yeasen, 20431ES08), 300 ng E1- 181 UBA1 (Targetmol, TMPY-02840), and 500 ng Article Title: TRIM39 aggravates hepatocellular carcinoma growth through targeting the p62-KEAP1-NRF2 axis Article Snippet: .. Briefly, Purified recombinant His-p62 and GST-TRIM39 proteins were incubated in a mixture of ubiquitin ligation reaction containing 5 mM MgCl2, 50 mM Tris-HCl (pH 7.5), 2 mM DTT, 4 mM ATP, 1 μg ubiquitin (Yeasen, 20431ES08), 300 ng E1-UBA1 (Targetmol, TMPY-02840), and 500 ng Incubation:Article Title: TRIM39 aggravates hepatocellular carcinoma growth through targeting the p62-KEAP1-NRF2 axis. Article Snippet: .. Briefly, Purified recombinant His-p62 and GST- 178 TRIM39 proteins were incubated in a mixture of ubiquitin ligation 179 reaction containing 5 mM MgCl2, 50 mM Tris-HCl (pH 7.5), 2 mM 180 DTT, 4 mM ATP, 1 μg ubiquitin (Yeasen, 20431ES08), 300 ng E1- 181 UBA1 (Targetmol, TMPY-02840), and 500 ng Article Title: TRIM39 aggravates hepatocellular carcinoma growth through targeting the p62-KEAP1-NRF2 axis Article Snippet: .. Briefly, Purified recombinant His-p62 and GST-TRIM39 proteins were incubated in a mixture of ubiquitin ligation reaction containing 5 mM MgCl2, 50 mM Tris-HCl (pH 7.5), 2 mM DTT, 4 mM ATP, 1 μg ubiquitin (Yeasen, 20431ES08), 300 ng E1-UBA1 (Targetmol, TMPY-02840), and 500 ng Ubiquitin Proteomics:Article Title: TRIM39 aggravates hepatocellular carcinoma growth through targeting the p62-KEAP1-NRF2 axis. Article Snippet: .. Briefly, Purified recombinant His-p62 and GST- 178 TRIM39 proteins were incubated in a mixture of ubiquitin ligation 179 reaction containing 5 mM MgCl2, 50 mM Tris-HCl (pH 7.5), 2 mM 180 DTT, 4 mM ATP, 1 μg ubiquitin (Yeasen, 20431ES08), 300 ng E1- 181 UBA1 (Targetmol, TMPY-02840), and 500 ng Article Title: TRIM39 aggravates hepatocellular carcinoma growth through targeting the p62-KEAP1-NRF2 axis Article Snippet: .. Briefly, Purified recombinant His-p62 and GST-TRIM39 proteins were incubated in a mixture of ubiquitin ligation reaction containing 5 mM MgCl2, 50 mM Tris-HCl (pH 7.5), 2 mM DTT, 4 mM ATP, 1 μg ubiquitin (Yeasen, 20431ES08), 300 ng E1-UBA1 (Targetmol, TMPY-02840), and 500 ng Ligation:Article Title: TRIM39 aggravates hepatocellular carcinoma growth through targeting the p62-KEAP1-NRF2 axis. Article Snippet: .. Briefly, Purified recombinant His-p62 and GST- 178 TRIM39 proteins were incubated in a mixture of ubiquitin ligation 179 reaction containing 5 mM MgCl2, 50 mM Tris-HCl (pH 7.5), 2 mM 180 DTT, 4 mM ATP, 1 μg ubiquitin (Yeasen, 20431ES08), 300 ng E1- 181 UBA1 (Targetmol, TMPY-02840), and 500 ng Article Title: TRIM39 aggravates hepatocellular carcinoma growth through targeting the p62-KEAP1-NRF2 axis Article Snippet: .. Briefly, Purified recombinant His-p62 and GST-TRIM39 proteins were incubated in a mixture of ubiquitin ligation reaction containing 5 mM MgCl2, 50 mM Tris-HCl (pH 7.5), 2 mM DTT, 4 mM ATP, 1 μg ubiquitin (Yeasen, 20431ES08), 300 ng E1-UBA1 (Targetmol, TMPY-02840), and 500 ng SDS Page:Article Title: TRIM39 aggravates hepatocellular carcinoma growth through targeting the p62-KEAP1-NRF2 axis. Article Snippet: .. Briefly, Purified recombinant His-p62 and GST- 178 TRIM39 proteins were incubated in a mixture of ubiquitin ligation 179 reaction containing 5 mM MgCl2, 50 mM Tris-HCl (pH 7.5), 2 mM 180 DTT, 4 mM ATP, 1 μg ubiquitin (Yeasen, 20431ES08), 300 ng E1- 181 UBA1 (Targetmol, TMPY-02840), and 500 ng Article Title: TRIM39 aggravates hepatocellular carcinoma growth through targeting the p62-KEAP1-NRF2 axis Article Snippet: .. Briefly, Purified recombinant His-p62 and GST-TRIM39 proteins were incubated in a mixture of ubiquitin ligation reaction containing 5 mM MgCl2, 50 mM Tris-HCl (pH 7.5), 2 mM DTT, 4 mM ATP, 1 μg ubiquitin (Yeasen, 20431ES08), 300 ng E1-UBA1 (Targetmol, TMPY-02840), and 500 ng Western Blot:Article Title: TRIM39 aggravates hepatocellular carcinoma growth through targeting the p62-KEAP1-NRF2 axis. Article Snippet: .. Briefly, Purified recombinant His-p62 and GST- 178 TRIM39 proteins were incubated in a mixture of ubiquitin ligation 179 reaction containing 5 mM MgCl2, 50 mM Tris-HCl (pH 7.5), 2 mM 180 DTT, 4 mM ATP, 1 μg ubiquitin (Yeasen, 20431ES08), 300 ng E1- 181 UBA1 (Targetmol, TMPY-02840), and 500 ng Article Title: TRIM39 aggravates hepatocellular carcinoma growth through targeting the p62-KEAP1-NRF2 axis Article Snippet: .. Briefly, Purified recombinant His-p62 and GST-TRIM39 proteins were incubated in a mixture of ubiquitin ligation reaction containing 5 mM MgCl2, 50 mM Tris-HCl (pH 7.5), 2 mM DTT, 4 mM ATP, 1 μg ubiquitin (Yeasen, 20431ES08), 300 ng E1-UBA1 (Targetmol, TMPY-02840), and 500 ng |
![Figure 1. In vitro reconstitution of STAM1 ubiquitination by AIP4. A, STAM1 ubiquitination by AIP4 was reconstituted in vitro and performed in the presence of increasing concentrations of βarrestin1. Ubiquitination reactions comprised of E1(Ube1, 42 nM), E2 <t>(UbcH7,</t> 350 nM), E3 (AIP4, 48.5 nM), ubiquitin (11.6 μM), DTT (1 mM), ATP (1 mM) plus STAM1 (42 nM), and varying concentrations of βarr1 (0 nM, 20 nM, 40 nM, 80 nM, 160 nM, or 320 nM) in 40 μl. Reactions were incubated for 90 min at 37 C and terminated with 40 μl 2× sample buffer. Equal volumes were analyzed by 7% SDS-PAGE and immunoblotting with the indicated antibodies. Polyubiquitinated [Ub(n)] and unmodified STAM1 are indicated. Immunoblots are from one representative experiment. STAM1 ubiquitination was quantified from the STAM (B) and ubiquitin (C) immunoblots using densitometry and shown as line graphs. Data were expressed relative to the signal at 300 nM βarr1 and represent the mean ± S.D. from three independent experiments. Curves were fitted by nonlinear regression, Michaelis–Menten (GraphPad Prism). AIP4, atrophin-interacting protein 4; STAM, signal-transducing adaptor molecule.](https://pub-med-unpaywalled-images-cdn.bioz.com/pub_med_ids_ending_with_1209/pm37981209/pm37981209__page2_image1.jpg)