Journal: Cell Communication and Signaling : CCS

Article Title: Trophoblast derived extracellular vesicles trigger secondary vesicle release from endometrial epithelial cells

doi: 10.1186/s12964-026-03096-4

Figure Lengend Snippet: Different chemical modulators related to EV uptake

Article Snippet: Dynasore (S80470), Chlorpromazine (S574902), Heparin sodium (S1346), Genistein (S1342), Wortmannin (S2758) were purchased from Selleckchem.

Techniques: Binding Assay, Blocking Assay, Activity Assay

Journal: Cell Communication and Signaling : CCS

Article Title: Trophoblast derived extracellular vesicles trigger secondary vesicle release from endometrial epithelial cells

doi: 10.1186/s12964-026-03096-4

Figure Lengend Snippet: Endocytosis inhibition attenuated the EV mediated embryo maternal cross-talk. a-b The in vitro embryo implantation model and the experimental design used to study EV mediated embryo maternal cross-talk. c Various EV uptake pathways and the mechanisms of action of the chemical inhibitors used to block each pathway. d-i Evaluation of cytotoxicity at the selected inhibitor concentrations. DMSO (chlorpromazine, dynasore, wortmannin, cytochalasin D and genistein) or sterile water (heparin) was used as the vehicle control. j-o RL95-2 cells were pre-treated with selected concentrations of chlorpromazine (10 µM), dynasore (20 µM), wortmannin (1 µM), heparin (20 µg/mL), cytochalasin D (10 µM), and genistein (50 µM) for 30 min and then the cells were treated with JAr EVs at 10 9 EVs/mL concentration along with fresh inhibitor for another 30 min. The MFGE8 concentration in cell culture supernatants were measured using MFGE8 ELISA. The results from three or more independent experiments were summarized as the mean ± SD ( n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: Dynasore (S80470), Chlorpromazine (S574902), Heparin sodium (S1346), Genistein (S1342), Wortmannin (S2758) were purchased from Selleckchem.

Techniques: Inhibition, In Vitro, Blocking Assay, Sterility, Control, Concentration Assay, Cell Culture, Enzyme-linked Immunosorbent Assay