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Image Search Results
Journal: Asian journal of nanoscience and materials
Article Title: Effects of fluorodeoxyglucose magnetic nanoparticles on NCI-H727 and SH-SY5Y cancer cells
doi: 10.26655/AJNANOMAT.2021.1.5
Figure Lengend Snippet: Analysis of morphological changes to NCI-H727 spheroids after FDG-mNP treatment and Hydroxy Dynasore treatment A) Spheroids with an appropriate sphericity index (SI) were selected and treated with FDG-mNPs, with or without Hydroxy Dynasore 24 hours prior to FDG-mNP treatment, and an EVOS XL microscope was used to obtain images. B) Spheroids were reconstructed using the ReVISP software (https://sourceforge.net/projects/revisp/). C) Quantitative morphological information of the spheroids, including sphericity index (SI), roundness, and solidity. ImageJ software (https://imagej.net/) was used along with Macro S1 for the calculation of morphological parameters. Samples labeled as “control” received no treatment, samples labeled as “FDG-MNPs” were treated with FDG-mNPs only, and samples labeled as “Hydroxy Dynasore” were treated with Hydroxy Dynasore prior to FDG-mNP treatment
Article Snippet: Fasentin and
Techniques: Microscopy, Software, Labeling, Control
Journal: Asian journal of nanoscience and materials
Article Title: Effects of fluorodeoxyglucose magnetic nanoparticles on NCI-H727 and SH-SY5Y cancer cells
doi: 10.26655/AJNANOMAT.2021.1.5
Figure Lengend Snippet: Fluorescence microscopy of NCI-H727 and corrected total cell fluorescence (CTCF). NCI-H727 cells were cultured for 24 hours to allow formation of spheroids and were treated with A) FDG-mNPs only or B) FDG-mNPs and Hydroxy Dynasore. The resulting spheroids were stained with propidium iodide solution (nucleus of dead cells appear red) in 100 mL of media for 10 minutes. Images were acquired using a Widefield microscope at 493 nm. C) CTCF was calculated using the formula Area×Intensity Density-Background using the ImageJ software
Article Snippet: Fasentin and
Techniques: Fluorescence, Microscopy, Cell Culture, Staining, Software