scf  (R&D Systems)

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Impairment of dendritic cell functionality and steady-state number in obese mice.

doi: 10.4049/jimmunol.177.9.5997

Figure Lengend Snippet: FIGURE 1. Immune responsiveness to KLH of ob/ob mice. A, Spleen cells from KLH-immunized control (CT, ) and ob/ob (f) mice were cultured with 0–50 g/ml KLH (five mice per group). Each stimulatory condition was done in triplicate. Results are expressed as mean of [methyl-3H]thymidine incorporation SEM. †, p 0.05 by a two-way ANOVA. B, Spleen cells from KLH-immunized control () and ob/ob (f) mice were cultured with 0 or 1 g/ml Con A (five mice per group). Each stimulatory condition was done in triplicate. Results are expressed as mean of [methyl-3H]thymidine incorporation SEM. C, Determination by ELISA of IL-10 and IFN- concentrations in culture supernatants of KLH-immunized control () and ob/ob (f) mice. All tests were done in duplicate. D, Determination by ELISA of total IgG, IgG1, and IgG2a anti-KLH Ab levels in individual sera of nonimmunized and KLH-immunized control () and ob/ob (f) mice. All tests were done in duplicate. Student’s t test: , p 0.05. E, Determination by FACS of percentages of DCs in the spleen of nonimmunized control (left panel) and ob/ob mice (right panel). Cells were double-stained using anti- FITC-coupled MHCII and PE-coupled anti-CD11c Abs. The experiment was performed on five animals, and the difference between both groups was significant using the Student t test; , p 0.05.

Article Snippet: Leptin concentration was determined using the ELISA kit DuoSet mouse leptin (R&D Systems).

Techniques: Control, Cell Culture, Enzyme-linked Immunosorbent Assay, Staining

Journal: Clinical and Vaccine Immunology

Article Title: DNA Vaccine Using Mycobacterium bovis Ag85B Antigen Induces Partial Protection against Experimental Infection in BALB/c Mice

doi: 10.1128/cvi.00151-06

Figure Lengend Snippet: FIG. 2. Total anti-Ag85B-specific IgG responses of mice immunized with DNA vaccine. Animals (n 10) were injected i.m. with DNA vaccine construct pCI-Ag85B, or control plasmid pCI. DNA immunization was performed at days 0, 15, 30, and 45, and specific IgG levels to Ag85B were assayed by ELISA at days 15, 30, 45, and 60 after each vaccination in a working dilution of 1:100. Results are expressed as means of each group immunized. Error bars indicate standard deviations of the means. Statistically significant differences to pCI control group are denoted by an asterisk (P 0.05). OD, optical density.

Article Snippet: After 72 h at 37°C in air with 5% CO2, splenocyte culture supernatants were tested for the presence of cytokines with the ELISA kit DuoSet (R&D Systems, MN) (mouse TNF- , IL-4, and IFN- ).

Techniques: Injection, Construct, Control, Plasmid Preparation, Enzyme-linked Immunosorbent Assay