dusp16 (Cell Signaling Technology Inc)
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dusp16
Dusp16, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dusp16/DUSP16%2FMKP7+Rabbit+mAb/pmc12085810-91-9-11
Average 90 stars, based on 8 article reviews
Dusp16, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dusp16/DUSP16%2FMKP7+Rabbit+mAb/pmc12085810-91-9-11
Average 90 stars, based on 8 article reviews
dusp16 - by Bioz Stars,
2026-09
90/100 stars
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1) Product Images from "FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway"
Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway
Journal: Cancer Cell International
doi: 10.1186/s12935-025-03808-9
Figure Legend Snippet: FBXL18 targeted DUSP16 as a ubiquitination substrate. ( A-B ) The results of Co-IP and western blotting assays revealed the endogenous and exogenous interaction of FBXL18 and DUSP16. ( C-D ) qRT-PCR analysis of DUSP16 mRNA levels after silencing or overexpressing FBXL18 in KLE and Ishikawa cells. ( E-F ) Western blot analysis of DUSP16 protein level in EC cells of shNC and shFBXL18 groups, and quantitative analysis. ( G-H ) Western blot analysis of DUSP16 protein level in EC cells after overexpressing FBXL18, and quantitative analysis. ( I-J ) DUSP16 protein half-life in EC cells of LV-Control and LV-FBXL18 groups were evaluated by CHX chase assay. ( K-L ) Comparison of DUSP16 protein half-life in EC cells stably knockdown of FBXL18 and control cells. ( M-N ) The ubiquitination status of endogenous DUSP16 after silencing or overexpressing FBXL18 in EC cells were determined by immunoprecipitation and western blotting. * P < 0.05; ** P < 0.01; *** P < 0.001
Techniques Used: Ubiquitin Proteomics, Co-Immunoprecipitation Assay, Western Blot, Quantitative RT-PCR, Control, Comparison, Stable Transfection, Knockdown, Immunoprecipitation
Figure Legend Snippet: FBXL18 promoted EC cell malignancy via DUSP16-mediated activation of JNK signaling. ( A-C ) Western blot analysis of DUSP16, JNK, p-JNK, c-JUN, and p-c-JUN levels in EC cells of siNeg and siDUSP16 groups, and quantitative analysis. ( D-F ) Western blot analysis of DUSP16, JNK, p-JNK, c-JUN, and p-c-JUN levels in EC cells after overexpressing DUSP16, and quantitative analysis. ( G-I ) Western blot analysis of DUSP16, JNK, p-JNK, c-JUN, and p-c-JUN levels in EC cells transfected with LV-Ctrl + Vector, LV-Ctrl + DUSP16, LV-FBXL18 + Vector, LV-FBXL18 + DUSP16, and quantitative analysis. ( J-L ) Western blot analysis of DUSP16, JNK, p-JNK, c-JUN, and p-c-JUN levels in EC cells transfected with shNC + siNeg, shNC + siDUSP16, shFBXL18 + siNeg, shFBXL18 + siDUSP16, and quantitative analysis. * P < 0.05; ** P < 0.01; *** P < 0.001
Techniques Used: Activation Assay, Western Blot, Transfection, Plasmid Preparation
Figure Legend Snippet: Silence of FBXL18 inhibited EC growth in vivo. ( A ) Tumor volumes of shNC and shFBXL18 groups were determined at different defined time points. ( B ) Representative images of xenograft tumors in shNC and shFBXL18 groups. ( C ) Tumor weights of shNC and shFBXL18 groups were measured at the end point. ( E ) Western blot analysis of FBXL18, DUSP16, JNK, p-JNK, c-JUN, p-c-JUN, and EMT-related markers in tumor samples of shNC and shFBXL18 groups, and quantitative analysis. ( F ) qRT-PCR analysis of FBXL18 and DUSP16 in tumor samples of shNC and shFBXL18 groups. * P < 0.05; ** P < 0.01; *** P < 0.001
Techniques Used: In Vivo, Western Blot, Quantitative RT-PCR
Related Articles
Ubiquitin Proteomics:Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway. Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: MAP3K1 and MAP2K4 mutations are associated with sensitivity to MEK inhibitors in multiple cancer models Article Snippet: Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.. Antibodies against p-JNK (T183/Y185) (4668), JNK (9252), p-JUN (S63) (2361), JUN (2315), MAP2K4 (9152), DUSP4 (5149), Co-Immunoprecipitation Assay:Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway. Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: MAP3K1 and MAP2K4 mutations are associated with sensitivity to MEK inhibitors in multiple cancer models Article Snippet: Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.. Antibodies against p-JNK (T183/Y185) (4668), JNK (9252), p-JUN (S63) (2361), JUN (2315), MAP2K4 (9152), DUSP4 (5149), Western Blot:Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway. Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: MAP3K1 and MAP2K4 mutations are associated with sensitivity to MEK inhibitors in multiple cancer models Article Snippet: Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.. Antibodies against p-JNK (T183/Y185) (4668), JNK (9252), p-JUN (S63) (2361), JUN (2315), MAP2K4 (9152), DUSP4 (5149), Quantitative RT-PCR:Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway. Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: MAP3K1 and MAP2K4 mutations are associated with sensitivity to MEK inhibitors in multiple cancer models Article Snippet: Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.. Antibodies against p-JNK (T183/Y185) (4668), JNK (9252), p-JUN (S63) (2361), JUN (2315), MAP2K4 (9152), DUSP4 (5149), Control:Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway. Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: MAP3K1 and MAP2K4 mutations are associated with sensitivity to MEK inhibitors in multiple cancer models Article Snippet: Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.. Antibodies against p-JNK (T183/Y185) (4668), JNK (9252), p-JUN (S63) (2361), JUN (2315), MAP2K4 (9152), DUSP4 (5149), Comparison:Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway. Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: MAP3K1 and MAP2K4 mutations are associated with sensitivity to MEK inhibitors in multiple cancer models Article Snippet: Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.. Antibodies against p-JNK (T183/Y185) (4668), JNK (9252), p-JUN (S63) (2361), JUN (2315), MAP2K4 (9152), DUSP4 (5149), Stable Transfection:Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway. Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: MAP3K1 and MAP2K4 mutations are associated with sensitivity to MEK inhibitors in multiple cancer models Article Snippet: Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.. Antibodies against p-JNK (T183/Y185) (4668), JNK (9252), p-JUN (S63) (2361), JUN (2315), MAP2K4 (9152), DUSP4 (5149), Knockdown:Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway. Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: MAP3K1 and MAP2K4 mutations are associated with sensitivity to MEK inhibitors in multiple cancer models Article Snippet: Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.. Antibodies against p-JNK (T183/Y185) (4668), JNK (9252), p-JUN (S63) (2361), JUN (2315), MAP2K4 (9152), DUSP4 (5149), Immunoprecipitation:Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway. Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: MAP3K1 and MAP2K4 mutations are associated with sensitivity to MEK inhibitors in multiple cancer models Article Snippet: Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.. Antibodies against p-JNK (T183/Y185) (4668), JNK (9252), p-JUN (S63) (2361), JUN (2315), MAP2K4 (9152), DUSP4 (5149), Activation Assay:Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway. Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: MAP3K1 and MAP2K4 mutations are associated with sensitivity to MEK inhibitors in multiple cancer models Article Snippet: Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.. Antibodies against p-JNK (T183/Y185) (4668), JNK (9252), p-JUN (S63) (2361), JUN (2315), MAP2K4 (9152), DUSP4 (5149), Transfection:Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway. Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: MAP3K1 and MAP2K4 mutations are associated with sensitivity to MEK inhibitors in multiple cancer models Article Snippet: Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.. Antibodies against p-JNK (T183/Y185) (4668), JNK (9252), p-JUN (S63) (2361), JUN (2315), MAP2K4 (9152), DUSP4 (5149), Plasmid Preparation:Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway. Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: MAP3K1 and MAP2K4 mutations are associated with sensitivity to MEK inhibitors in multiple cancer models Article Snippet: Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.. Antibodies against p-JNK (T183/Y185) (4668), JNK (9252), p-JUN (S63) (2361), JUN (2315), MAP2K4 (9152), DUSP4 (5149), In Vivo:Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway. Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: MAP3K1 and MAP2K4 mutations are associated with sensitivity to MEK inhibitors in multiple cancer models Article Snippet: Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.. Antibodies against p-JNK (T183/Y185) (4668), JNK (9252), p-JUN (S63) (2361), JUN (2315), MAP2K4 (9152), DUSP4 (5149), Negative Control:Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway. Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: MAP3K1 and MAP2K4 mutations are associated with sensitivity to MEK inhibitors in multiple cancer models Article Snippet: Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.. Antibodies against p-JNK (T183/Y185) (4668), JNK (9252), p-JUN (S63) (2361), JUN (2315), MAP2K4 (9152), DUSP4 (5149), Knock-Out:Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway. Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: MAP3K1 and MAP2K4 mutations are associated with sensitivity to MEK inhibitors in multiple cancer models Article Snippet: Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.. Antibodies against p-JNK (T183/Y185) (4668), JNK (9252), p-JUN (S63) (2361), JUN (2315), MAP2K4 (9152), DUSP4 (5149), Expressing:Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway. Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: MAP3K1 and MAP2K4 mutations are associated with sensitivity to MEK inhibitors in multiple cancer models Article Snippet: Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.. Antibodies against p-JNK (T183/Y185) (4668), JNK (9252), p-JUN (S63) (2361), JUN (2315), MAP2K4 (9152), DUSP4 (5149), Transduction:Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway. Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: MAP3K1 and MAP2K4 mutations are associated with sensitivity to MEK inhibitors in multiple cancer models Article Snippet: Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.. Antibodies against p-JNK (T183/Y185) (4668), JNK (9252), p-JUN (S63) (2361), JUN (2315), MAP2K4 (9152), DUSP4 (5149), Over Expression:Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway. Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: MAP3K1 and MAP2K4 mutations are associated with sensitivity to MEK inhibitors in multiple cancer models Article Snippet: Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.. Antibodies against p-JNK (T183/Y185) (4668), JNK (9252), p-JUN (S63) (2361), JUN (2315), MAP2K4 (9152), DUSP4 (5149), Cell Culture:Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.The next day, the resultant mixtures were incubated with the pre-treated protein A + G agarose beads (CST, USA) at 4 °C with gently rotation for 2 h. After centrifuging at 2000 rpm for 5 min, the supernatant was discarded and the precipitates were collected for subsequent western blot analysis.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway. Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: FBXL18 promotes endometrial carcinoma progression via destabilizing DUSP16 and thus activating JNK signaling pathway Article Snippet: GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.GAPDH protein level was employed for normalization, and the relative protein expression level was determined by ImageJ software.. The primary antibodies included FBXL18 (sc-100738, Santa Cruz, USA; 1:500), Article Title: MAP3K1 and MAP2K4 mutations are associated with sensitivity to MEK inhibitors in multiple cancer models Article Snippet: Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.Selumetinib (S1008), trametinib (S2673), SCH772984 (S7101), JNK-IN-8 (S4901) and dacomitinib (S2727) were purchased from Selleck Chemicals.. Antibodies against p-JNK (T183/Y185) (4668), JNK (9252), p-JUN (S63) (2361), JUN (2315), MAP2K4 (9152), DUSP4 (5149), |
