draq5 (Miltenyi Biotec)
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Miltenyi Biotec
draq5
Draq5, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/draq5+staining+solution/DRAQ5+Staining+Solution/pm41745542-139-33-34
Average 97 stars, based on 9 article reviews
Draq5, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/draq5+staining+solution/DRAQ5+Staining+Solution/pm41745542-139-33-34
Average 97 stars, based on 9 article reviews
draq5 - by Bioz Stars,
2026-09
97/100 stars
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Staining:Article Title: Cholesterol remodels the endoplasmic reticulum to control myofibroblastic CAF function Article Snippet: Fixed cells were washed with PBS and stained with freshly prepared 50 μg/mL Filipin III (Sigma-Aldrich) in PBS for 1 hour at room temperature in the dark. .. Filipin was removed, and cells were washed and imaged in PBS containing 5 μM Article Title: Cell wall damage increases macromolecular crowding effects in the Escherichia coli cytoplasm Article Snippet: DAPI for nucleic acid staining , Sigma-Aldrich , CAS: 28718-90-3. .. Article Title: HDAC1-3 inhibition increases SARS-CoV-2 replication and productive infection in lung mesothelial and epithelial cells Article Snippet: Cy3-conjugated anti-rat secondary antibodies (Jackson ImmunoResearch, 112-165-003). .. Article Title: Mechanisms of mesothelial cell response to viral infections: HDAC1-3 inhibition blocks poly(I:C)-induced type I interferon response and modulates the mesenchymal/inflammatory phenotype Article Snippet: Antibodies for immunofluorescence include the following: anti-FN 1 (ABCAM, ab2413) and anti-calnexin (Santa Cruz Biotechnology, sc-23954), Cy3-conjugated anti-rat secondary antibodies (Jackson ImmunoResearch, 112-165-003), and anti-mouse Alexa Fluor 488-conjugated (A32723 Thermo Fischer Scientific, Waltham, MA, USA). .. Microscopy:Article Title: Cholesterol remodels the endoplasmic reticulum to control myofibroblastic CAF function Article Snippet: Fixed cells were washed with PBS and stained with freshly prepared 50 μg/mL Filipin III (Sigma-Aldrich) in PBS for 1 hour at room temperature in the dark. .. Filipin was removed, and cells were washed and imaged in PBS containing 5 μM |
![(A) Measurement of the relative incorporation of [U- 14 C]glucose into protein, non-polar, RNA, DNA and polar fractions following 24 hours of labelling, n=3. (B) Relative incorporation of [1- 14 C]acetate into the lipid fraction of NFs and CAFs, n=3. (C) Gene Set Enrichment Analysis (GSEA) plot derived from RNA-Seq analysis of CAFs versus NFs, showing enrichment of a “lipid metabolic process” signature (GO:0006629). (D) Heatmap of cholesterol biosynthesis gene expression in NFs and CAFs, as determined by RNA-Seq analysis, n=3. (E) Quantification of relative total cholesterol levels in NFs and CAFs, n=3. (F) UMAP visualisation of 19,601 patient-derived breast tumour stromal cells analysed by single cell RNA-Seq (scRNA-Seq) from the Human Breast Cancer Atlas . Stromal clusters corresponding to immunomodulatory CAFs (iCAFs), myCAFs, perivascular (PVL) and endothelial cell subsets are indicated by colour (top). Feature plots illustrating expression of SQLE (bottom left) and SC5D (bottom right) in breast tumour stromal cell clusters. Gene expression is represented by log-normalised expression values. The myCAF cluster is delineated by a dashed line. (G) Representative filipin staining (green) of free cholesterol in NFs and CAFs. Nuclei are stained with <t>Draq5</t> (blue). Dashed boxes indicate magnified regions shown to the right. White scale bar represents 10 µm, yellow scale bar represents 5 µm. (H) Quantification of relative cholesterol levels in the ER-enriched fraction isolated from NFs and CAFs, n=3.](https://bio-rxiv-images-cdn.bioz.com/dois_ending_with_37/10__64898_slash_2026__02__16__706237/10__64898_slash_2026__02__16__706237___F1.large.jpg)