Review



antibodies against dnmt3a  (Novus Biologicals)


Bioz Verified Symbol Novus Biologicals is a verified supplier
Bioz Manufacturer Symbol Novus Biologicals manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Novus Biologicals antibodies against dnmt3a
    Antibodies Against Dnmt3a, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dnmt3a/HES-1+Antibody+(OTI4H1)/pm42014700-311-9-13
    Average 90 stars, based on 12 article reviews
    antibodies against dnmt3a - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    other:

    Article Title: Ionizing radiation expands a p90RSK-activated patrolling monocyte subset: modulation by colchicine
    Article Snippet: 170Er , DNMT3A , Novus , NB120-13888 (0.1 mg) , I.

    Clone Assay:

    Article Title: An efficient framework to decipher microRNA regulatory programs applied to T cells
    Article Snippet: For intracellular staining, the FoxP3 buffer fixation kit (Life technologies) was used according to manufacturer’s instructions. .. For intracellular miR-29 targets, Dnmt3a and Hp1α, antibodies were purchased from Novus Biologicals (NB120-13888PE, NBP2-52434), clones 64B1446 and 3A11F8, respectively. .. Dnmt3a antibody was purchased as a PE conjugate and Hp1α staining was performed by primary antibody staining followed by secondary Alexa647 goat-anti mouse antibody (Invitrogen A21236) staining.

    Article Title: An efficient framework to decipher microRNA regulatory programs applied to T cells.
    Article Snippet: For intracellular staining, the FoxP3 buffer fixation kit (Life technologies) was used according to manufacturer’s instructions. .. For intracellular miR-29 targets, Dnmt3a and Hp1α, antibodies were purchased from Novus Biologicals (NB120-13888PE, NBP2-52434), clones 64B1446 and 3A11F8, respectively. .. Dnmt3a antibody was purchased as a PE conjugate and Hp1α staining was performed by primary antibody staining followed by secondary Alexa647 goat-anti mouse antibody (Invitrogen A21236) staining.

    Incubation:

    Article Title: The Association Between Clinical Outcome and Expression of DNMT1, 3A, and 3B in Locally Advanced Laryngeal Carcinomas Treated by Definitive Radiotherapy.
    Article Snippet: .. The slides were incubated for 1 h with mouse monoclonal antibodies (DNMT1 (1:100, Clone 60B1220.1, Novus Biologicals, Centennial, CO, USA), DNMT3A (1:200, Clone 64B1446, Novus Biologicals) and DNMT3B (1:100, clone 52A1018, Novus Biologicals)) and subsequently incubated for 30 min with EnVision, a peroxidase-conjugated polymer backbone. ..

    Article Title: The Association Between Clinical Outcome and Expression of DNMT1, 3A, and 3B in Locally Advanced Laryngeal Carcinomas Treated by Definitive Radiotherapy
    Article Snippet: .. The slides were incubated for 1 h with mouse monoclonal antibodies (DNMT1 (1:100, Clone 60B1220.1, Novus Biologicals, Centennial, CO, USA), DNMT3A (1:200, Clone 64B1446, Novus Biologicals) and DNMT3B (1:100, clone 52A1018, Novus Biologicals)) and subsequently incubated for 30 min with EnVision, a peroxidase-conjugated polymer backbone. ..

    Article Title: Differential CpG methylation at Nnat in the early establishment of beta cell heterogeneity
    Article Snippet: Sections (10 m) were cut using a CM1950 Cryostat (Leica) and permeabilised with 0.2% Triton X-100 in PBS, followed by blocking in 2% (w/v) BSA and 5% (v/v) chicken serum in PBS containing 0.1% Tween-20. .. Sections were immunostained using primary antibodies diluted in blocking buffer against NNAT (clone EPR13554(B), ab181353 from Abcam, 1:1000), GFP (GFP-1010, Aves Labs, 1:3000) INS (clone K36AC10, I2018, Sigma-Aldrich, 1:5000), somatostatin (SST) (13-2366, American Research Products, 1:1000), glucagon (GCG) (clone K79bB10, G2654, Sigma-Aldrich, 1:3000) urocortin 3 (UCN3) (a kind gift from Joan Vaughan and Paul E. Sawchenko, The Salk Institute for Biological Studies, PBL #7218 [32] 1:1000), translocase of outer mitochondrial membrane (TOM20) (FL-145, sc-11415, Santa Cruz Biotechnology, 1:250), DNMT1 (clone 60B1220.1, NB100-56519, Novus Biologicals, 1:500) and DNMT3A (clone 64B1446, NB12013888, Novus Biologicals, 1:500) with overnight incubation at 4oC followed by incubation with secondary antibody (Alexa Fluor, Thermo Fisher Scientific, 1:500) and counterstaining with DAPI (0.1 g/mL). .. Sections were imaged using a TCS SP5 confocal microscope (Leica, Germany), capturing multiple islets from at least three regions of tissue >100m apart.

    Article Title: DNA methylation modulates nucleosome retention in sperm and H3K4 methylation deposition in early mouse embryos
    Article Snippet: Rehydrated tubules were blocked in PBST containing 4% Normal Donkey Serum (Abcam ab7475) for 1 hour. .. After blocking, the tubules were incubated with primary antibodies against cKit (1:1000 R&D systems AF1356), DNMT3A (1:1000 Imgenex IMG-268A) and DNMT3B (1:1000 Imgenex IMG-184A) for 3 hours at room temperature. .. Afterwards, the tubules were washed 3 times for 10 min in blocking buffer followed by incubation with species specific secondary Alexafluor-conjugated antibodies (1:1000, ThermoFischer Scientific) for 2 hours.

    Bioprocessing:

    Article Title: The Association Between Clinical Outcome and Expression of DNMT1, 3A, and 3B in Locally Advanced Laryngeal Carcinomas Treated by Definitive Radiotherapy.
    Article Snippet: .. The slides were incubated for 1 h with mouse monoclonal antibodies (DNMT1 (1:100, Clone 60B1220.1, Novus Biologicals, Centennial, CO, USA), DNMT3A (1:200, Clone 64B1446, Novus Biologicals) and DNMT3B (1:100, clone 52A1018, Novus Biologicals)) and subsequently incubated for 30 min with EnVision, a peroxidase-conjugated polymer backbone. ..

    Article Title: The Association Between Clinical Outcome and Expression of DNMT1, 3A, and 3B in Locally Advanced Laryngeal Carcinomas Treated by Definitive Radiotherapy
    Article Snippet: .. The slides were incubated for 1 h with mouse monoclonal antibodies (DNMT1 (1:100, Clone 60B1220.1, Novus Biologicals, Centennial, CO, USA), DNMT3A (1:200, Clone 64B1446, Novus Biologicals) and DNMT3B (1:100, clone 52A1018, Novus Biologicals)) and subsequently incubated for 30 min with EnVision, a peroxidase-conjugated polymer backbone. ..

    Polymer:

    Article Title: The Association Between Clinical Outcome and Expression of DNMT1, 3A, and 3B in Locally Advanced Laryngeal Carcinomas Treated by Definitive Radiotherapy.
    Article Snippet: .. The slides were incubated for 1 h with mouse monoclonal antibodies (DNMT1 (1:100, Clone 60B1220.1, Novus Biologicals, Centennial, CO, USA), DNMT3A (1:200, Clone 64B1446, Novus Biologicals) and DNMT3B (1:100, clone 52A1018, Novus Biologicals)) and subsequently incubated for 30 min with EnVision, a peroxidase-conjugated polymer backbone. ..

    Article Title: The Association Between Clinical Outcome and Expression of DNMT1, 3A, and 3B in Locally Advanced Laryngeal Carcinomas Treated by Definitive Radiotherapy
    Article Snippet: .. The slides were incubated for 1 h with mouse monoclonal antibodies (DNMT1 (1:100, Clone 60B1220.1, Novus Biologicals, Centennial, CO, USA), DNMT3A (1:200, Clone 64B1446, Novus Biologicals) and DNMT3B (1:100, clone 52A1018, Novus Biologicals)) and subsequently incubated for 30 min with EnVision, a peroxidase-conjugated polymer backbone. ..

    Blocking Assay:

    Article Title: Chronic corticosterone exposure in rats induces sex-specific alterations in hypothalamic reelin fragments, MeCP2, and DNMT3a protein levels.
    Article Snippet: .. Primary antibodies diluted in blocking buffer probing for reelin (1:1000, MILLIPORE, MAB5364), MeCP2 (1:10,000, M9317, Sigma-Aldrich), DNMT3a (1:1000, Novus bio, 64B1446) and the normalization proteins or loading controls, B-actin (1:5000, SigmaAldrich) and H4 (1:10,000 antibody produced in house). ..

    Article Title: Differential CpG methylation at Nnat in the early establishment of beta cell heterogeneity
    Article Snippet: Sections (10 m) were cut using a CM1950 Cryostat (Leica) and permeabilised with 0.2% Triton X-100 in PBS, followed by blocking in 2% (w/v) BSA and 5% (v/v) chicken serum in PBS containing 0.1% Tween-20. .. Sections were immunostained using primary antibodies diluted in blocking buffer against NNAT (clone EPR13554(B), ab181353 from Abcam, 1:1000), GFP (GFP-1010, Aves Labs, 1:3000) INS (clone K36AC10, I2018, Sigma-Aldrich, 1:5000), somatostatin (SST) (13-2366, American Research Products, 1:1000), glucagon (GCG) (clone K79bB10, G2654, Sigma-Aldrich, 1:3000) urocortin 3 (UCN3) (a kind gift from Joan Vaughan and Paul E. Sawchenko, The Salk Institute for Biological Studies, PBL #7218 [32] 1:1000), translocase of outer mitochondrial membrane (TOM20) (FL-145, sc-11415, Santa Cruz Biotechnology, 1:250), DNMT1 (clone 60B1220.1, NB100-56519, Novus Biologicals, 1:500) and DNMT3A (clone 64B1446, NB12013888, Novus Biologicals, 1:500) with overnight incubation at 4oC followed by incubation with secondary antibody (Alexa Fluor, Thermo Fisher Scientific, 1:500) and counterstaining with DAPI (0.1 g/mL). .. Sections were imaged using a TCS SP5 confocal microscope (Leica, Germany), capturing multiple islets from at least three regions of tissue >100m apart.

    Article Title: DNA methylation modulates nucleosome retention in sperm and H3K4 methylation deposition in early mouse embryos
    Article Snippet: Rehydrated tubules were blocked in PBST containing 4% Normal Donkey Serum (Abcam ab7475) for 1 hour. .. After blocking, the tubules were incubated with primary antibodies against cKit (1:1000 R&D systems AF1356), DNMT3A (1:1000 Imgenex IMG-268A) and DNMT3B (1:1000 Imgenex IMG-184A) for 3 hours at room temperature. .. Afterwards, the tubules were washed 3 times for 10 min in blocking buffer followed by incubation with species specific secondary Alexafluor-conjugated antibodies (1:1000, ThermoFischer Scientific) for 2 hours.

    Produced:

    Article Title: Chronic corticosterone exposure in rats induces sex-specific alterations in hypothalamic reelin fragments, MeCP2, and DNMT3a protein levels.
    Article Snippet: .. Primary antibodies diluted in blocking buffer probing for reelin (1:1000, MILLIPORE, MAB5364), MeCP2 (1:10,000, M9317, Sigma-Aldrich), DNMT3a (1:1000, Novus bio, 64B1446) and the normalization proteins or loading controls, B-actin (1:5000, SigmaAldrich) and H4 (1:10,000 antibody produced in house). ..

    Membrane:

    Article Title: Differential CpG methylation at Nnat in the early establishment of beta cell heterogeneity
    Article Snippet: Sections (10 m) were cut using a CM1950 Cryostat (Leica) and permeabilised with 0.2% Triton X-100 in PBS, followed by blocking in 2% (w/v) BSA and 5% (v/v) chicken serum in PBS containing 0.1% Tween-20. .. Sections were immunostained using primary antibodies diluted in blocking buffer against NNAT (clone EPR13554(B), ab181353 from Abcam, 1:1000), GFP (GFP-1010, Aves Labs, 1:3000) INS (clone K36AC10, I2018, Sigma-Aldrich, 1:5000), somatostatin (SST) (13-2366, American Research Products, 1:1000), glucagon (GCG) (clone K79bB10, G2654, Sigma-Aldrich, 1:3000) urocortin 3 (UCN3) (a kind gift from Joan Vaughan and Paul E. Sawchenko, The Salk Institute for Biological Studies, PBL #7218 [32] 1:1000), translocase of outer mitochondrial membrane (TOM20) (FL-145, sc-11415, Santa Cruz Biotechnology, 1:250), DNMT1 (clone 60B1220.1, NB100-56519, Novus Biologicals, 1:500) and DNMT3A (clone 64B1446, NB12013888, Novus Biologicals, 1:500) with overnight incubation at 4oC followed by incubation with secondary antibody (Alexa Fluor, Thermo Fisher Scientific, 1:500) and counterstaining with DAPI (0.1 g/mL). .. Sections were imaged using a TCS SP5 confocal microscope (Leica, Germany), capturing multiple islets from at least three regions of tissue >100m apart.



    Similar Products

    94
    Bioss p dnmt3a
    P Dnmt3a, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dnmt3a/phospho-Dnmt3a+(Ser390+%2B+Ser393)+Polyclonal+Antibody/pm41929572-91-18-19
    Average 94 stars, based on 1 article reviews
    p dnmt3a - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    90
    Novus Biologicals antibodies against dnmt3a
    Antibodies Against Dnmt3a, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dnmt3a/HES-1+Antibody+(OTI4H1)/pm42014700-311-9-13
    Average 90 stars, based on 1 article reviews
    antibodies against dnmt3a - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    93
    Novus Biologicals dnmt3a
    Dnmt3a, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dnmt3a/DNMT3A+Antibody+(64B1446)+-+BSA+Free/pmc13124628-22-2-4
    Average 93 stars, based on 1 article reviews
    dnmt3a - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Novus Biologicals nb120 13888
    Nb120 13888, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dnmt3a/DNMT3A+Antibody+(64B1446)+-+BSA+Free/pmc13124628-22-6-4
    Average 93 stars, based on 1 article reviews
    nb120 13888 - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    94
    Bioss antibodies against dnmt3a
    Antibodies Against Dnmt3a, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dnmt3a/DNMT-3+alpha+Polyclonal+Antibody/pm41929572-91-7-10
    Average 94 stars, based on 1 article reviews
    antibodies against dnmt3a - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    93
    Novus Biologicals nb120
    Nb120, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dnmt3a/DNMT3A+Antibody+(64B1446)+-+BSA+Free/pmc12957900-96-0-3
    Average 93 stars, based on 1 article reviews
    nb120 - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    94
    Novus Biologicals anti dnmt3a
    Anti Dnmt3a, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dnmt3a/DNMT3A+Antibody/pmc12957900-95-58-60
    Average 94 stars, based on 1 article reviews
    anti dnmt3a - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    93
    Novus Biologicals anti dnmt3b
    Anti Dnmt3b, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dnmt3a/DNMT3A+Antibody+(64B1446)+-+BSA+Free/pmc12957900-96-1-3
    Average 93 stars, based on 1 article reviews
    anti dnmt3b - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    94
    Bioss α sma
    GDL treatment improves liver function and fibrosis-related serological indices in WD model rats. A Serum levels of hyaluronic acid (HA), laminin (LN), type III pro-collagen (PC-III), collagen IV (C-IV), aspartate aminotransferase (ALT), and alanine aminotransferase (AST) in each group ( n = 6). B Hepatic copper content across different groups ( n = 6). C Protein expression <t>of</t> <t>α-SMA</t> and collagen I in rat liver tissues, as determined by Western blotting ( n = 3). D Protein expression <t>of</t> <t>α-SMA</t> and collagen I in LX-2 cells treated with CuSO₄ and GDL-containing serum ( n = 3). GAPDH was used as a loading control. Data are presented as means ± SD. Statistical significance was determined by one-way ANOVA with Bonferroni’s post hoc test. ## p < 0.01 vs. control group; * p < 0.05, ** p < 0.01 vs. model group; * p < 0.05, ** p < 0.01 vs. CuSO 4 group
    α Sma, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dnmt3a/phospho-Dnmt3a+(Ser390+%2B+Ser393)+Polyclonal+Antibody/pmc13038811-102-25-26
    Average 94 stars, based on 1 article reviews
    α sma - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    Image Search Results


    GDL treatment improves liver function and fibrosis-related serological indices in WD model rats. A Serum levels of hyaluronic acid (HA), laminin (LN), type III pro-collagen (PC-III), collagen IV (C-IV), aspartate aminotransferase (ALT), and alanine aminotransferase (AST) in each group ( n = 6). B Hepatic copper content across different groups ( n = 6). C Protein expression of α-SMA and collagen I in rat liver tissues, as determined by Western blotting ( n = 3). D Protein expression of α-SMA and collagen I in LX-2 cells treated with CuSO₄ and GDL-containing serum ( n = 3). GAPDH was used as a loading control. Data are presented as means ± SD. Statistical significance was determined by one-way ANOVA with Bonferroni’s post hoc test. ## p < 0.01 vs. control group; * p < 0.05, ** p < 0.01 vs. model group; * p < 0.05, ** p < 0.01 vs. CuSO 4 group

    Journal: 3 Biotech

    Article Title: Gandouling protects against hepatic fibrosis in Wilson disease through the lncRNA-SNHG7/miR-29b/DNMT3A pathway

    doi: 10.1007/s13205-026-04769-0

    Figure Lengend Snippet: GDL treatment improves liver function and fibrosis-related serological indices in WD model rats. A Serum levels of hyaluronic acid (HA), laminin (LN), type III pro-collagen (PC-III), collagen IV (C-IV), aspartate aminotransferase (ALT), and alanine aminotransferase (AST) in each group ( n = 6). B Hepatic copper content across different groups ( n = 6). C Protein expression of α-SMA and collagen I in rat liver tissues, as determined by Western blotting ( n = 3). D Protein expression of α-SMA and collagen I in LX-2 cells treated with CuSO₄ and GDL-containing serum ( n = 3). GAPDH was used as a loading control. Data are presented as means ± SD. Statistical significance was determined by one-way ANOVA with Bonferroni’s post hoc test. ## p < 0.01 vs. control group; * p < 0.05, ** p < 0.01 vs. model group; * p < 0.05, ** p < 0.01 vs. CuSO 4 group

    Article Snippet: The membranes were then incubated overnight with antibodies against DNMT3A (Bioss, Item No. bs-23029R, 1:2000, Rabbit, 1:1000, Rabbit), p-DNMT3A (Bioss, Item No. bs-14399R, 1:2000, Rabbit), α-SMA (Bioss, Item No. Bsm-33187 M, 1:2000, Mouse; No. bs-0189R, 1:1000, Rabbit), Collagen I (Bioss, Item No. bs-7158R, 1:1000, Rabbit; No. AB260043 , 1:1000, Rabbit), Beclin-1 (Abcam, Item No. ab210498, 1:2000, Rabbit; No. ab62472, 1:1000, Rabbit), LC3B (CST, Item No. 3868s, 1:1000, Rabbit; No. 43566 S, 1:1000, Rabbit), and p62 (Bioss, Item No. bs-2951R, 1:1000, Rabbit) at 4°C with gentle shaking.

    Techniques: Expressing, Western Blot, Control