Journal: Vaccines

Article Title: Mycobacterium avium subsp. paratuberculosis Candidate Vaccine Strains Are Pro-apoptotic in RAW 264.7 Murine Macrophages

doi: 10.3390/vaccines11061085

Figure Lengend Snippet: Determination of apoptosis using a rapid staining assay. RAW 264.7 uninfected control (gray) and MAP-infected cells were stained with DAPI at 6 h post-infection: ( A ) the percent of apoptotic nuclei is displayed. The asterisk color represents the strain to which pairwise comparisons were statistically significant: *** p ≤ 0.001. Pairwise comparisons are presented for DMAP56 (top) and DMAP52 (bottom) vs. the control, wild types, and 4H2 (upper line). The lower line shows pairwise comparisons between 4H2 (top), UNL K-10 (inline), and NADC K-10 (bottom) vs. the uninfected cells. Results represent means ( n = 3) ± standard errors of the mean; ( B ) phase contrast (left) and fluorescent (right) micrographs for representative samples for the uninfected cells and each strain. These images were merged using Adobe Photoshop Version 24.4.1 (San Jose, CA, USA) and modified for uniformity and clarity.

Article Snippet: The percent of apoptosis was quantified via characteristic nuclear morphology and visualized via treatment with the fluorescent DNA-binding dye DAPI (4′,6-diamidino-2-phenylindole) (MilliporeSigmaTM CalbiochemTM; Burlington, MA, USA).

Techniques: Staining, Control, Infection, Modification

Journal: iScience

Article Title: IL-1beta promotes the age-associated decline of beta cell function

doi: 10.1016/j.isci.2021.103250

Figure Lengend Snippet:

Article Snippet: DNA-binding dye DAPI , SIGMA , 10,236,276,001.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Software