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dapi (4’, 6-diamidino-2-phenylindole, fluorescent dna-binding dye  (Millipore)


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    Millipore dapi (4’, 6-diamidino-2-phenylindole, fluorescent dna-binding dye
    Dapi (4’, 6 Diamidino 2 Phenylindole, Fluorescent Dna Binding Dye, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna-binding+dye+dapi/dapi/pm37550525-64-34-40
    Average 90 stars, based on 1 article reviews
    dapi (4’, 6-diamidino-2-phenylindole, fluorescent dna-binding dye - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Fusion protein comprising IL13
    Article Snippet: .. Cells are incubated with rabbit anti-β3 tubulin (Anti-beta III Tubulin antibody-Neuronal Marker, ab18207, 1:1500, Abcam, Cambridge, UK) and NeuN (Anti-NeuN Antibody clone A60, MAB377, 1:500) Sigma Aldrich (Merck), Darmstadt, Germany) overnight at 4° C., followed by washes and incubation with AF488-conjugated donkey anti-rabbit and 568-conjugated donkey anti-mouse secondary antibodies (Thermofisher, 1:500) followed by DAPI (1:5000, Sigma) staining before sections are mounted on slides with FluorSave reagent (Millipore). .. Images are taken using an Olympus IX83 microscope (Olympus).

    Article Title: Fusion protein comprising IL13
    Article Snippet: .. Cells were incubated with rabbit anti-β3 tubulin (Anti-beta III Tubulin antibody-Neuronal Marker, ab18207, 1:1500, Abcam, Cambridge, UK) and NeuN (Anti-NeuN Antibody clone A60, MAB377, 1:500) Sigma Aldrich (Merck), Darmstadt, Germany) overnight at 4° C., followed by washes and incubation with AF488-conjugated donkey anti-rabbit and 568-conjugated donkey anti-mouse secondary antibodies (Thermofisher, 1:500) followed by DAPI (1:5000, Sigma) staining before sections were mounted on slides with FluorSave reagent (Millipore). .. Images were taken using an Olympus IX83 microscope (Olympus).

    Marker:

    Article Title: Fusion protein comprising IL13
    Article Snippet: .. Cells are incubated with rabbit anti-β3 tubulin (Anti-beta III Tubulin antibody-Neuronal Marker, ab18207, 1:1500, Abcam, Cambridge, UK) and NeuN (Anti-NeuN Antibody clone A60, MAB377, 1:500) Sigma Aldrich (Merck), Darmstadt, Germany) overnight at 4° C., followed by washes and incubation with AF488-conjugated donkey anti-rabbit and 568-conjugated donkey anti-mouse secondary antibodies (Thermofisher, 1:500) followed by DAPI (1:5000, Sigma) staining before sections are mounted on slides with FluorSave reagent (Millipore). .. Images are taken using an Olympus IX83 microscope (Olympus).

    Article Title: Fusion protein comprising IL13
    Article Snippet: .. Cells were incubated with rabbit anti-β3 tubulin (Anti-beta III Tubulin antibody-Neuronal Marker, ab18207, 1:1500, Abcam, Cambridge, UK) and NeuN (Anti-NeuN Antibody clone A60, MAB377, 1:500) Sigma Aldrich (Merck), Darmstadt, Germany) overnight at 4° C., followed by washes and incubation with AF488-conjugated donkey anti-rabbit and 568-conjugated donkey anti-mouse secondary antibodies (Thermofisher, 1:500) followed by DAPI (1:5000, Sigma) staining before sections were mounted on slides with FluorSave reagent (Millipore). .. Images were taken using an Olympus IX83 microscope (Olympus).

    Staining:

    Article Title: Fusion protein comprising IL13
    Article Snippet: .. Cells are incubated with rabbit anti-β3 tubulin (Anti-beta III Tubulin antibody-Neuronal Marker, ab18207, 1:1500, Abcam, Cambridge, UK) and NeuN (Anti-NeuN Antibody clone A60, MAB377, 1:500) Sigma Aldrich (Merck), Darmstadt, Germany) overnight at 4° C., followed by washes and incubation with AF488-conjugated donkey anti-rabbit and 568-conjugated donkey anti-mouse secondary antibodies (Thermofisher, 1:500) followed by DAPI (1:5000, Sigma) staining before sections are mounted on slides with FluorSave reagent (Millipore). .. Images are taken using an Olympus IX83 microscope (Olympus).

    Article Title: Fusion protein comprising IL13
    Article Snippet: .. Cells were incubated with rabbit anti-β3 tubulin (Anti-beta III Tubulin antibody-Neuronal Marker, ab18207, 1:1500, Abcam, Cambridge, UK) and NeuN (Anti-NeuN Antibody clone A60, MAB377, 1:500) Sigma Aldrich (Merck), Darmstadt, Germany) overnight at 4° C., followed by washes and incubation with AF488-conjugated donkey anti-rabbit and 568-conjugated donkey anti-mouse secondary antibodies (Thermofisher, 1:500) followed by DAPI (1:5000, Sigma) staining before sections were mounted on slides with FluorSave reagent (Millipore). .. Images were taken using an Olympus IX83 microscope (Olympus).

    Fluorsave:

    Article Title: Fusion protein comprising IL13
    Article Snippet: .. Cells are incubated with rabbit anti-β3 tubulin (Anti-beta III Tubulin antibody-Neuronal Marker, ab18207, 1:1500, Abcam, Cambridge, UK) and NeuN (Anti-NeuN Antibody clone A60, MAB377, 1:500) Sigma Aldrich (Merck), Darmstadt, Germany) overnight at 4° C., followed by washes and incubation with AF488-conjugated donkey anti-rabbit and 568-conjugated donkey anti-mouse secondary antibodies (Thermofisher, 1:500) followed by DAPI (1:5000, Sigma) staining before sections are mounted on slides with FluorSave reagent (Millipore). .. Images are taken using an Olympus IX83 microscope (Olympus).

    Article Title: Fusion protein comprising IL13
    Article Snippet: .. Cells were incubated with rabbit anti-β3 tubulin (Anti-beta III Tubulin antibody-Neuronal Marker, ab18207, 1:1500, Abcam, Cambridge, UK) and NeuN (Anti-NeuN Antibody clone A60, MAB377, 1:500) Sigma Aldrich (Merck), Darmstadt, Germany) overnight at 4° C., followed by washes and incubation with AF488-conjugated donkey anti-rabbit and 568-conjugated donkey anti-mouse secondary antibodies (Thermofisher, 1:500) followed by DAPI (1:5000, Sigma) staining before sections were mounted on slides with FluorSave reagent (Millipore). .. Images were taken using an Olympus IX83 microscope (Olympus).

    other:

    Article Title: Regulatory T Cells Boost Efficacy of Post-Infarction Pluripotent Stem Cell-Derived Cardiovascular Progenitor Cell Transplants.
    Article Snippet: The number of capillaries were counted and expressed as the number of capillaries per square millimetre (mm2) using a staining Griffonia Bandeiraea Simplicifolia Lectin I (20 mg/mL, Vector, CA, USA) conjugated with rhodamine, in conjunction with the nuclear marker 4′,6-diamidino-2-phenylindole (1 mg/mL, DAPI, Calbiochem, France).

    Article Title: Supplemental Information DOT1L interaction partner AF10 controls patterning of H3K79 methyla- tion and RNA polymerase II to maintain cell identity
    Article Snippet: Coverslips were washed once, incubated with 4′,6-Diamidino-2-phenylindole dihydrochloride (1:10,000; Millipore Sigma, D8417) in wash buffer, and then washed a second time before mounting with Aqua-Poly/Mount (Fisher Scientific, NC9439247).

    Article Title: A type I interferon regulatory network for human plasmacytoid dendritic cells based on heparin, membrane-bound and soluble BDCA-2
    Article Snippet: GL Sciences Inc. Cat# GL-5020- 75000 IMDM, w/o: L-Glutamine, w: 25 mM HEPES, w: 3.024 g/L NaHCO3 PAN BIOTECH Cat# P04-20050 Insulin-Transferrin-Selenium (ITS -G) (100X) GIBCO Cat# 41400045 Table S3. (continued) MEM Vitamin Solution (100X) GIBCO Cat# 11120037 MEM Amino Acids Solution (50X) GIBCO Cat# 11130036 rmSCF Markus Schnare, Germany N/A Albumin from human serum Sigma-Aldrich Cat# A3782-100MG Fibrinogen from human plasma Sigma-Aldrich Cat# F3879-100MG Bio-Pure Human Serum Albumin (HSA) 10% solution Sartorius Cat# 05-720-1D Recombinant P. vulgaris Chondroitinase ABC Protein, CF R&D systems Cat# 6877-GH-020 Bacteroides Heparinase I New england biolabs Cat# P0735S Bacteroides Heparinase II New england biolabs Cat# P0736S Bacteroides Heparinase III New england biolabs Cat# P0737S Perchloric acid ACS reagent, 70% Sigma-Aldrich Cat# 244252-100ML 4–20% Mini-PROTEAN® TGXTM Precast Protein Gels, 12-well, 20 μl #4561095 Biorad Cat# 4561095 Toluidine Blue O Sigma-Aldrich Cat# T3260 Flt3-L Supernatant produced within Department for Immunology N/A PageBlueTM Protein Staining Solution Thermo Fisher Scientific Cat# 24620 mIFN-α Supernatant produced within Department for Immunology N/A Recombinant Mouse M-CSF Protein R&D systems Cat# 416-ML Amicon Ultra - 4 Millipore Cat# UFC801024 Strep-Tactin® HRP conjugate IBA Cat# 2-1502-001 DAPI Sigma-Aldrich Cat# D9542 Perchloric acid Honeywell Cat# 244252 Ammonium hydroxide solution Honeywell Cat# 221228 Critical commercial assays Plasmacytoid Dendritic Cell Isolation Kit II Miltenyi Biotec Cat# 130-097-415 Human DLEC/CLEC4C/BDCA2 ELISA Kit Invitrogen Cat# EH156RB Anti IIa Assay Kit Kinetichrome Cat# IIAI IFN alpha Human Matched Antibody Pair Invitrogen Cat# BMS216MST Protein Labeling Kit RED-MALEIMIDE 2nd Generation (Cysteine reactive) Nanotemper Cat# MO-L014 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Protease inhibitor Cocktail kit Thermo Fisher Scientific Cat# 78410 2216 CpG 20-mer TIB MOLBIOL N/A CpG2216_Cy3 Biospring Cat# 290536_G pcDNA3.1(-)_sol-hBDCA2 This work GenBank accession# OQ817991 Table S3. (continued) pcDNA3.1(-)_sol-hBDCA2_N110Q This work GenBank accession# OQ817992 pcDNA3.1(-)_sol-hBDCA2_N137Q This work GenBank accession# OQ817993 pcDNA3.1(-)_sol-hBDCA2_N164Q _ This work GenBank accession# OQ817994 pcDNA3.1(-)_sol-hBDCA2_E172A/N174A This work GenBank accession# OQ817995 pcDNA3.1(-)_sol-hDCIR This work GenBank accession# OQ817996 pcDNA3.1(-)_sol-hDECTIN-2 This work GenBank accession# OQ817997 pcDNA3.1(-)_sol-hIL-8 This work GenBank accession# OQ817998

    Article Title: Unique recognition, phagocytosis, and intracellular survival of Cryptococcus neoformans in a human immortalized microglia cell line
    Article Snippet: Coverslips were washed with PBS and a corresponding Alexa Fluor-conjugated goat secondary antibody (Invitrogen) was diluted to 1 μg/mL in PBS with 2 μg/mL of 4’6-diamidino-2-phenylindole (DAPI; Millipore Sigma) and added to cells for 1 hour at RT.

    Labeling:

    Article Title: Hypoxia-Induced Metabolic Reprogramming and Markings of Cell Fate in Concentric Arterial Hypertrophy
    Article Snippet: The cell pellet was resuspended in 1.5 mL of resuspension buffer: PBS, 1% FBS, 1 mM EDTA, DNase I (MilliporeSigma). .. The dead cells were labeled with DAPI (MilliporeSigma). .. Cells were analyzed and sorted using an Influx Cell Sorter (Becton Dickinson) at the Flow Cytometry Core Facility of the University of Virginia.



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    Image Search Results


    Determination of apoptosis using a rapid staining assay. RAW 264.7 uninfected control (gray) and MAP-infected cells were stained with DAPI at 6 h post-infection: ( A ) the percent of apoptotic nuclei is displayed. The asterisk color represents the strain to which pairwise comparisons were statistically significant: *** p ≤ 0.001. Pairwise comparisons are presented for DMAP56 (top) and DMAP52 (bottom) vs. the control, wild types, and 4H2 (upper line). The lower line shows pairwise comparisons between 4H2 (top), UNL K-10 (inline), and NADC K-10 (bottom) vs. the uninfected cells. Results represent means ( n = 3) ± standard errors of the mean; ( B ) phase contrast (left) and fluorescent (right) micrographs for representative samples for the uninfected cells and each strain. These images were merged using Adobe Photoshop Version 24.4.1 (San Jose, CA, USA) and modified for uniformity and clarity.

    Journal: Vaccines

    Article Title: Mycobacterium avium subsp. paratuberculosis Candidate Vaccine Strains Are Pro-apoptotic in RAW 264.7 Murine Macrophages

    doi: 10.3390/vaccines11061085

    Figure Lengend Snippet: Determination of apoptosis using a rapid staining assay. RAW 264.7 uninfected control (gray) and MAP-infected cells were stained with DAPI at 6 h post-infection: ( A ) the percent of apoptotic nuclei is displayed. The asterisk color represents the strain to which pairwise comparisons were statistically significant: *** p ≤ 0.001. Pairwise comparisons are presented for DMAP56 (top) and DMAP52 (bottom) vs. the control, wild types, and 4H2 (upper line). The lower line shows pairwise comparisons between 4H2 (top), UNL K-10 (inline), and NADC K-10 (bottom) vs. the uninfected cells. Results represent means ( n = 3) ± standard errors of the mean; ( B ) phase contrast (left) and fluorescent (right) micrographs for representative samples for the uninfected cells and each strain. These images were merged using Adobe Photoshop Version 24.4.1 (San Jose, CA, USA) and modified for uniformity and clarity.

    Article Snippet: The percent of apoptosis was quantified via characteristic nuclear morphology and visualized via treatment with the fluorescent DNA-binding dye DAPI (4′,6-diamidino-2-phenylindole) (MilliporeSigmaTM CalbiochemTM; Burlington, MA, USA).

    Techniques: Staining, Control, Infection, Modification

    Journal: iScience

    Article Title: IL-1beta promotes the age-associated decline of beta cell function

    doi: 10.1016/j.isci.2021.103250

    Figure Lengend Snippet:

    Article Snippet: DNA-binding dye DAPI , SIGMA , 10,236,276,001.

    Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Software