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dna microarray laser scanner (dna microarray scanner ba  (Agilent technologies)


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    Agilent technologies dna microarray laser scanner (dna microarray scanner ba
    <t>Microarray</t> analysis of transfected HeLa cells with miR-141 overexpressing vector. A Microarray analysis of gene expression in HeLa cells transfected with miR-141 overexpression vs. cells transfected with the control vector using a miRNA library from Exiqon. B Microarray analysis of gene expression in HeLa cells transfected with miR-141 overexpression vs. cells transfected with the control vector using oligonucleotides from Ambion. The blue color indicates the sustained gene expression, the red indicates the upregulated genes, and the green indicates the downregulated genes. C The expression of the most relevant genes to cervical cancer indicates the upregulated genes in red columns and downregulated genes in green. Error bars indicate the STD between Exiqon and Ambion data. D The potential binding sites of miR-141 within the 3 − UTR of KLRC1 and coding sequences of KLRC3, CAM3, and that carried out in-silico by miRWalk online tool
    Dna Microarray Laser Scanner (Dna Microarray Scanner Ba, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+microarray+scanner/pmc11370040-147-7-15
    Average 90 stars, based on 1 article reviews
    dna microarray laser scanner (dna microarray scanner ba - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "Regulation of KLRC and Ceacam gene expression by miR-141 supports cell proliferation and metastasis in cervical cancer cells"

    Article Title: Regulation of KLRC and Ceacam gene expression by miR-141 supports cell proliferation and metastasis in cervical cancer cells

    Journal: BMC Cancer

    doi: 10.1186/s12885-024-12794-6

    Microarray analysis of transfected HeLa cells with miR-141 overexpressing vector. A Microarray analysis of gene expression in HeLa cells transfected with miR-141 overexpression vs. cells transfected with the control vector using a miRNA library from Exiqon. B Microarray analysis of gene expression in HeLa cells transfected with miR-141 overexpression vs. cells transfected with the control vector using oligonucleotides from Ambion. The blue color indicates the sustained gene expression, the red indicates the upregulated genes, and the green indicates the downregulated genes. C The expression of the most relevant genes to cervical cancer indicates the upregulated genes in red columns and downregulated genes in green. Error bars indicate the STD between Exiqon and Ambion data. D The potential binding sites of miR-141 within the 3 − UTR of KLRC1 and coding sequences of KLRC3, CAM3, and that carried out in-silico by miRWalk online tool
    Figure Legend Snippet: Microarray analysis of transfected HeLa cells with miR-141 overexpressing vector. A Microarray analysis of gene expression in HeLa cells transfected with miR-141 overexpression vs. cells transfected with the control vector using a miRNA library from Exiqon. B Microarray analysis of gene expression in HeLa cells transfected with miR-141 overexpression vs. cells transfected with the control vector using oligonucleotides from Ambion. The blue color indicates the sustained gene expression, the red indicates the upregulated genes, and the green indicates the downregulated genes. C The expression of the most relevant genes to cervical cancer indicates the upregulated genes in red columns and downregulated genes in green. Error bars indicate the STD between Exiqon and Ambion data. D The potential binding sites of miR-141 within the 3 − UTR of KLRC1 and coding sequences of KLRC3, CAM3, and that carried out in-silico by miRWalk online tool

    Techniques Used: Microarray, Transfection, Plasmid Preparation, Expressing, Over Expression, Control, Binding Assay, In Silico

    The prediction information between miR-141 and targeted genes identified by  microarray  analysis using in-silico miRWalk tool
    Figure Legend Snippet: The prediction information between miR-141 and targeted genes identified by microarray analysis using in-silico miRWalk tool

    Techniques Used: Microarray, Binding Assay

    Related Articles

    Microarray:

    Article Title: EfpA is required for regrowth of Mycobacterium tuberculosis following isoniazid exposure.
    Article Snippet: Following overnight incubation, the slides were washed in Oligo aCGH wash buffer 1 (Agilent) at room temperature for 5 minutes with agitation and then in Oligo aCGH wash buffer 2 (Agilent) at 37°C for 1 minute with agitation. .. Slides were scanned immediately using an Agilent DNA Microarray Scanner (with SureScan 2 μm High-Resolu tion Technology; G2505C). .. The microarrays used were custom BμG@S ‘TBv3_0_0’ arrays (accession number A-BUGS-41) that were produced by Agilent Technologies.

    Article Title: Effective delivery of miR-150-5p with nucleus pulposus cell-specific nanoparticles attenuates intervertebral disc degeneration
    Article Snippet: .. The slides were then incubated in an Agilent hybrid oven at 65 °C for 17 h, washed, fixed and scanned using an Agilent DNA microarray scanner. ..

    Article Title: ABTC-0904: targeting glioma stem cells in GBM: a phase 0/II study of hedgehog pathway inhibitor GDC-0449
    Article Snippet: The Cy5-labeled DNA (5 μg) was co-hybridized with the Cy3-labeled human male reference DNA (5 μg) on Agilent high density microarray using the hybridization and washing conditions from the Agilent Oligonucleotide Array-Based CGH protocol (v6.2) for Genomic DNA Analysis. .. Arrays were scanned with the Agilent DNA Microarray Scanner at a 3um scan resolution, and quantified with Feature Extraction 11.0.1.1. ..

    Article Title: Caspase-mediated processing of TRBP regulates apoptosis during viral infection
    Article Snippet: The quality of the total RNA was confirmed using the Bioanalyzer (Agilent Technologies), and cDNA and Cy-3-labeled RNA were synthesized using the Quick Amp Labeling kit for One Color (Agilent Technologies). .. Cy-3-labeled RNA was fragmented using the Gene Expression Hybridization Kit (Agilent Technologies) and hybridized to SurePrint G3 Human GE Microarray version 3 (Agilent Technologies) at 65°C for 17 h. After being washed, the microarray slide was scanned by DNA Microarray Scanner (Agilent Technologies) and the signals were quantified by Feature Extraction software (Agilent Technologies). ..

    Article Title: A network-based trans-omics approach for predicting synergistic drug combinations
    Article Snippet: After hybridization, microarrays were washed for 1 min at room temperature with GE Wash Buffer 1 (Agilent) and 1 min at 37 °C with GE Wash Buffer 2 (Agilent), then dried immediately. .. The slides were scanned immediately after washing using an Agilent DNA Microarray Scanner (G2505C) with a one-color scan setting for 8 × 60 K array slides (Scan Area 61 × 21.6 mm, Scan resolution 3 μm, Dye channel was set to Green and Green PMT was set to 100%). .. The scanned images were analyzed with Feature Extraction Software 10.7.1.1 (Agilent) using default parameters (protocol GE1_107_Sep09 and Grid: 028282_D_F_20110531) to obtain background subtracted and spatially detrended processed signal intensities.

    Article Title: RP11-874 J12.4 promotes erlotinib resistance in non-small cell lung cancer via increasing AXL expression.
    Article Snippet: EGFR tyrosine kinase inhibitor (TKI) resistance is a major challenge for EGFR-mutant non-small cell lung cancer (NSCLC) treatment.. Our previous work revealed that overexpression of AXL promoted EGFR-TKI resistance through epithelial-mesenchymal transition (EMT) in a subset of NSCLC patients.. Compared with erlotinib resistant and sensitive cells, RP11-874 J12.4 was upregulated in erlotinib-resistant NSCLC cells (HCC827-ER3).

    Article Title: Long-term exposure to diesel exhaust particles induces concordant changes in DNA methylation and transcriptome in human adenocarcinoma alveolar basal epithelial cells
    Article Snippet: The labeled cRNA was used for hybridization to the Agilent SurePrint G3 Human GE Microarray 8 × 60K (v.3.0). .. After hybridization, the microarray slide was washed, and then scanned with an Agilent DNA microarray scanner according to the manufacturer’s protocol. .. Fluorescence signal intensity was quantified from the scanned image using Feature Extraction software (Agilent Technologies).

    Incubation:

    Article Title: Effective delivery of miR-150-5p with nucleus pulposus cell-specific nanoparticles attenuates intervertebral disc degeneration
    Article Snippet: .. The slides were then incubated in an Agilent hybrid oven at 65 °C for 17 h, washed, fixed and scanned using an Agilent DNA microarray scanner. ..

    other:

    Article Title: Methods for assessing risk of developing a viral disease using a genetic test
    Article Snippet: After labeling, samples were combined and co-hybridized to Agilent 1M feature oligonucleotide microarrays, design ID 021529 (Agilent Product Number G4447A) using standard conditions (array Comparative Genomic Hybridization—aCGH).

    Extraction:

    Article Title: ABTC-0904: targeting glioma stem cells in GBM: a phase 0/II study of hedgehog pathway inhibitor GDC-0449
    Article Snippet: The Cy5-labeled DNA (5 μg) was co-hybridized with the Cy3-labeled human male reference DNA (5 μg) on Agilent high density microarray using the hybridization and washing conditions from the Agilent Oligonucleotide Array-Based CGH protocol (v6.2) for Genomic DNA Analysis. .. Arrays were scanned with the Agilent DNA Microarray Scanner at a 3um scan resolution, and quantified with Feature Extraction 11.0.1.1. ..

    Article Title: Caspase-mediated processing of TRBP regulates apoptosis during viral infection
    Article Snippet: The quality of the total RNA was confirmed using the Bioanalyzer (Agilent Technologies), and cDNA and Cy-3-labeled RNA were synthesized using the Quick Amp Labeling kit for One Color (Agilent Technologies). .. Cy-3-labeled RNA was fragmented using the Gene Expression Hybridization Kit (Agilent Technologies) and hybridized to SurePrint G3 Human GE Microarray version 3 (Agilent Technologies) at 65°C for 17 h. After being washed, the microarray slide was scanned by DNA Microarray Scanner (Agilent Technologies) and the signals were quantified by Feature Extraction software (Agilent Technologies). ..

    Gene Expression:

    Article Title: Caspase-mediated processing of TRBP regulates apoptosis during viral infection
    Article Snippet: The quality of the total RNA was confirmed using the Bioanalyzer (Agilent Technologies), and cDNA and Cy-3-labeled RNA were synthesized using the Quick Amp Labeling kit for One Color (Agilent Technologies). .. Cy-3-labeled RNA was fragmented using the Gene Expression Hybridization Kit (Agilent Technologies) and hybridized to SurePrint G3 Human GE Microarray version 3 (Agilent Technologies) at 65°C for 17 h. After being washed, the microarray slide was scanned by DNA Microarray Scanner (Agilent Technologies) and the signals were quantified by Feature Extraction software (Agilent Technologies). ..

    Hybridization:

    Article Title: Caspase-mediated processing of TRBP regulates apoptosis during viral infection
    Article Snippet: The quality of the total RNA was confirmed using the Bioanalyzer (Agilent Technologies), and cDNA and Cy-3-labeled RNA were synthesized using the Quick Amp Labeling kit for One Color (Agilent Technologies). .. Cy-3-labeled RNA was fragmented using the Gene Expression Hybridization Kit (Agilent Technologies) and hybridized to SurePrint G3 Human GE Microarray version 3 (Agilent Technologies) at 65°C for 17 h. After being washed, the microarray slide was scanned by DNA Microarray Scanner (Agilent Technologies) and the signals were quantified by Feature Extraction software (Agilent Technologies). ..

    Article Title: Long-term exposure to diesel exhaust particles induces concordant changes in DNA methylation and transcriptome in human adenocarcinoma alveolar basal epithelial cells
    Article Snippet: The labeled cRNA was used for hybridization to the Agilent SurePrint G3 Human GE Microarray 8 × 60K (v.3.0). .. After hybridization, the microarray slide was washed, and then scanned with an Agilent DNA microarray scanner according to the manufacturer’s protocol. .. Fluorescence signal intensity was quantified from the scanned image using Feature Extraction software (Agilent Technologies).

    Software:

    Article Title: Caspase-mediated processing of TRBP regulates apoptosis during viral infection
    Article Snippet: The quality of the total RNA was confirmed using the Bioanalyzer (Agilent Technologies), and cDNA and Cy-3-labeled RNA were synthesized using the Quick Amp Labeling kit for One Color (Agilent Technologies). .. Cy-3-labeled RNA was fragmented using the Gene Expression Hybridization Kit (Agilent Technologies) and hybridized to SurePrint G3 Human GE Microarray version 3 (Agilent Technologies) at 65°C for 17 h. After being washed, the microarray slide was scanned by DNA Microarray Scanner (Agilent Technologies) and the signals were quantified by Feature Extraction software (Agilent Technologies). ..



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    <t>Microarray</t> analysis of transfected HeLa cells with miR-141 overexpressing vector. A Microarray analysis of gene expression in HeLa cells transfected with miR-141 overexpression vs. cells transfected with the control vector using a miRNA library from Exiqon. B Microarray analysis of gene expression in HeLa cells transfected with miR-141 overexpression vs. cells transfected with the control vector using oligonucleotides from Ambion. The blue color indicates the sustained gene expression, the red indicates the upregulated genes, and the green indicates the downregulated genes. C The expression of the most relevant genes to cervical cancer indicates the upregulated genes in red columns and downregulated genes in green. Error bars indicate the STD between Exiqon and Ambion data. D The potential binding sites of miR-141 within the 3 − UTR of KLRC1 and coding sequences of KLRC3, CAM3, and that carried out in-silico by miRWalk online tool
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    Image Search Results


    Microarray analysis of transfected HeLa cells with miR-141 overexpressing vector. A Microarray analysis of gene expression in HeLa cells transfected with miR-141 overexpression vs. cells transfected with the control vector using a miRNA library from Exiqon. B Microarray analysis of gene expression in HeLa cells transfected with miR-141 overexpression vs. cells transfected with the control vector using oligonucleotides from Ambion. The blue color indicates the sustained gene expression, the red indicates the upregulated genes, and the green indicates the downregulated genes. C The expression of the most relevant genes to cervical cancer indicates the upregulated genes in red columns and downregulated genes in green. Error bars indicate the STD between Exiqon and Ambion data. D The potential binding sites of miR-141 within the 3 − UTR of KLRC1 and coding sequences of KLRC3, CAM3, and that carried out in-silico by miRWalk online tool

    Journal: BMC Cancer

    Article Title: Regulation of KLRC and Ceacam gene expression by miR-141 supports cell proliferation and metastasis in cervical cancer cells

    doi: 10.1186/s12885-024-12794-6

    Figure Lengend Snippet: Microarray analysis of transfected HeLa cells with miR-141 overexpressing vector. A Microarray analysis of gene expression in HeLa cells transfected with miR-141 overexpression vs. cells transfected with the control vector using a miRNA library from Exiqon. B Microarray analysis of gene expression in HeLa cells transfected with miR-141 overexpression vs. cells transfected with the control vector using oligonucleotides from Ambion. The blue color indicates the sustained gene expression, the red indicates the upregulated genes, and the green indicates the downregulated genes. C The expression of the most relevant genes to cervical cancer indicates the upregulated genes in red columns and downregulated genes in green. Error bars indicate the STD between Exiqon and Ambion data. D The potential binding sites of miR-141 within the 3 − UTR of KLRC1 and coding sequences of KLRC3, CAM3, and that carried out in-silico by miRWalk online tool

    Article Snippet: The microarray experiments were scanned using a DNA microarray laser scanner (DNA Microarray Scanner BA, Agilent Technologies) at 5 µm resolution using Ambion ship and Exiqon ship according to the manufacturer’s instructions.

    Techniques: Microarray, Transfection, Plasmid Preparation, Expressing, Over Expression, Control, Binding Assay, In Silico

    The prediction information between miR-141 and targeted genes identified by  microarray  analysis using in-silico miRWalk tool

    Journal: BMC Cancer

    Article Title: Regulation of KLRC and Ceacam gene expression by miR-141 supports cell proliferation and metastasis in cervical cancer cells

    doi: 10.1186/s12885-024-12794-6

    Figure Lengend Snippet: The prediction information between miR-141 and targeted genes identified by microarray analysis using in-silico miRWalk tool

    Article Snippet: The microarray experiments were scanned using a DNA microarray laser scanner (DNA Microarray Scanner BA, Agilent Technologies) at 5 µm resolution using Ambion ship and Exiqon ship according to the manufacturer’s instructions.

    Techniques: Microarray, Binding Assay

    Flow chart of microarray data analysis. The agilent single color microarray platform was used having more than 58,000 transcripts and further data processing was done using agilent GeneSpring software followed by pathway analysis using GSEA database. Numbers in parentheses illustrates number of pathways enriched in each category using GSEA database. DEGs-Differentially expressed genes, ANOVA-Analysis of variance, BP-Biological pathway, CC-Cellular component, MF-Molecular function.

    Journal: bioRxiv

    Article Title: Synovial fluid transcriptome dynamics in osteoarthritis progression: Implications in pathogenesis

    doi: 10.1101/2024.06.24.600143

    Figure Lengend Snippet: Flow chart of microarray data analysis. The agilent single color microarray platform was used having more than 58,000 transcripts and further data processing was done using agilent GeneSpring software followed by pathway analysis using GSEA database. Numbers in parentheses illustrates number of pathways enriched in each category using GSEA database. DEGs-Differentially expressed genes, ANOVA-Analysis of variance, BP-Biological pathway, CC-Cellular component, MF-Molecular function.

    Article Snippet: Following hybridization, the array slides were washed and scanned with an Agilent SureScan High-Resolution DNA Microarray Scanner.

    Techniques: Microarray, Software