dna microarray laser scanner (dna microarray scanner ba (Agilent technologies)
Structured Review

Dna Microarray Laser Scanner (Dna Microarray Scanner Ba, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+microarray+scanner/pmc11370040-147-7-15
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "Regulation of KLRC and Ceacam gene expression by miR-141 supports cell proliferation and metastasis in cervical cancer cells"
Article Title: Regulation of KLRC and Ceacam gene expression by miR-141 supports cell proliferation and metastasis in cervical cancer cells
Journal: BMC Cancer
doi: 10.1186/s12885-024-12794-6
Figure Legend Snippet: Microarray analysis of transfected HeLa cells with miR-141 overexpressing vector. A Microarray analysis of gene expression in HeLa cells transfected with miR-141 overexpression vs. cells transfected with the control vector using a miRNA library from Exiqon. B Microarray analysis of gene expression in HeLa cells transfected with miR-141 overexpression vs. cells transfected with the control vector using oligonucleotides from Ambion. The blue color indicates the sustained gene expression, the red indicates the upregulated genes, and the green indicates the downregulated genes. C The expression of the most relevant genes to cervical cancer indicates the upregulated genes in red columns and downregulated genes in green. Error bars indicate the STD between Exiqon and Ambion data. D The potential binding sites of miR-141 within the 3 − UTR of KLRC1 and coding sequences of KLRC3, CAM3, and that carried out in-silico by miRWalk online tool
Techniques Used: Microarray, Transfection, Plasmid Preparation, Expressing, Over Expression, Control, Binding Assay, In Silico
Figure Legend Snippet: The prediction information between miR-141 and targeted genes identified by microarray analysis using in-silico miRWalk tool
Techniques Used: Microarray, Binding Assay
Related Articles
Microarray:Article Title: EfpA is required for regrowth of Mycobacterium tuberculosis following isoniazid exposure. Article Snippet: Following overnight incubation, the slides were washed in Oligo aCGH wash buffer 1 (Agilent) at room temperature for 5 minutes with agitation and then in Oligo aCGH wash buffer 2 (Agilent) at 37°C for 1 minute with agitation. .. Slides were scanned immediately using an Article Title: Effective delivery of miR-150-5p with nucleus pulposus cell-specific nanoparticles attenuates intervertebral disc degeneration Article Snippet: .. The slides were then incubated in an Agilent hybrid oven at 65 °C for 17 h, washed, fixed and scanned using an Article Title: ABTC-0904: targeting glioma stem cells in GBM: a phase 0/II study of hedgehog pathway inhibitor GDC-0449 Article Snippet: The Cy5-labeled DNA (5 μg) was co-hybridized with the Cy3-labeled human male reference DNA (5 μg) on Agilent high density microarray using the hybridization and washing conditions from the Agilent Oligonucleotide Array-Based CGH protocol (v6.2) for Genomic DNA Analysis. .. Arrays were scanned with the Article Title: Caspase-mediated processing of TRBP regulates apoptosis during viral infection Article Snippet: The quality of the total RNA was confirmed using the Bioanalyzer (Agilent Technologies), and cDNA and Cy-3-labeled RNA were synthesized using the Quick Amp Labeling kit for One Color (Agilent Technologies). .. Cy-3-labeled RNA was fragmented using the Gene Expression Hybridization Kit (Agilent Technologies) and hybridized to SurePrint G3 Human GE Microarray version 3 (Agilent Technologies) at 65°C for 17 h. After being washed, the microarray slide was scanned by Article Title: A network-based trans-omics approach for predicting synergistic drug combinations Article Snippet: After hybridization, microarrays were washed for 1 min at room temperature with GE Wash Buffer 1 (Agilent) and 1 min at 37 °C with GE Wash Buffer 2 (Agilent), then dried immediately. .. The slides were scanned immediately after washing using an Article Title: RP11-874 J12.4 promotes erlotinib resistance in non-small cell lung cancer via increasing AXL expression. Article Snippet: EGFR tyrosine kinase inhibitor (TKI) resistance is a major challenge for EGFR-mutant non-small cell lung cancer (NSCLC) treatment.. Our previous work revealed that overexpression of AXL promoted EGFR-TKI resistance through epithelial-mesenchymal transition (EMT) in a subset of NSCLC patients.. Compared with erlotinib resistant and sensitive cells, RP11-874 J12.4 was upregulated in erlotinib-resistant NSCLC cells (HCC827-ER3). Article Title: Long-term exposure to diesel exhaust particles induces concordant changes in DNA methylation and transcriptome in human adenocarcinoma alveolar basal epithelial cells Article Snippet: The labeled cRNA was used for hybridization to the Agilent SurePrint G3 Human GE Microarray 8 × 60K (v.3.0). .. After hybridization, the microarray slide was washed, and then scanned with an Incubation:Article Title: Effective delivery of miR-150-5p with nucleus pulposus cell-specific nanoparticles attenuates intervertebral disc degeneration Article Snippet: .. The slides were then incubated in an Agilent hybrid oven at 65 °C for 17 h, washed, fixed and scanned using an other:Article Title: Methods for assessing risk of developing a viral disease using a genetic test Article Snippet: After labeling, samples were combined and co-hybridized to Agilent 1M feature oligonucleotide microarrays, Extraction:Article Title: ABTC-0904: targeting glioma stem cells in GBM: a phase 0/II study of hedgehog pathway inhibitor GDC-0449 Article Snippet: The Cy5-labeled DNA (5 μg) was co-hybridized with the Cy3-labeled human male reference DNA (5 μg) on Agilent high density microarray using the hybridization and washing conditions from the Agilent Oligonucleotide Array-Based CGH protocol (v6.2) for Genomic DNA Analysis. .. Arrays were scanned with the Article Title: Caspase-mediated processing of TRBP regulates apoptosis during viral infection Article Snippet: The quality of the total RNA was confirmed using the Bioanalyzer (Agilent Technologies), and cDNA and Cy-3-labeled RNA were synthesized using the Quick Amp Labeling kit for One Color (Agilent Technologies). .. Cy-3-labeled RNA was fragmented using the Gene Expression Hybridization Kit (Agilent Technologies) and hybridized to SurePrint G3 Human GE Microarray version 3 (Agilent Technologies) at 65°C for 17 h. After being washed, the microarray slide was scanned by Gene Expression:Article Title: Caspase-mediated processing of TRBP regulates apoptosis during viral infection Article Snippet: The quality of the total RNA was confirmed using the Bioanalyzer (Agilent Technologies), and cDNA and Cy-3-labeled RNA were synthesized using the Quick Amp Labeling kit for One Color (Agilent Technologies). .. Cy-3-labeled RNA was fragmented using the Gene Expression Hybridization Kit (Agilent Technologies) and hybridized to SurePrint G3 Human GE Microarray version 3 (Agilent Technologies) at 65°C for 17 h. After being washed, the microarray slide was scanned by Hybridization:Article Title: Caspase-mediated processing of TRBP regulates apoptosis during viral infection Article Snippet: The quality of the total RNA was confirmed using the Bioanalyzer (Agilent Technologies), and cDNA and Cy-3-labeled RNA were synthesized using the Quick Amp Labeling kit for One Color (Agilent Technologies). .. Cy-3-labeled RNA was fragmented using the Gene Expression Hybridization Kit (Agilent Technologies) and hybridized to SurePrint G3 Human GE Microarray version 3 (Agilent Technologies) at 65°C for 17 h. After being washed, the microarray slide was scanned by Article Title: Long-term exposure to diesel exhaust particles induces concordant changes in DNA methylation and transcriptome in human adenocarcinoma alveolar basal epithelial cells Article Snippet: The labeled cRNA was used for hybridization to the Agilent SurePrint G3 Human GE Microarray 8 × 60K (v.3.0). .. After hybridization, the microarray slide was washed, and then scanned with an Software:Article Title: Caspase-mediated processing of TRBP regulates apoptosis during viral infection Article Snippet: The quality of the total RNA was confirmed using the Bioanalyzer (Agilent Technologies), and cDNA and Cy-3-labeled RNA were synthesized using the Quick Amp Labeling kit for One Color (Agilent Technologies). .. Cy-3-labeled RNA was fragmented using the Gene Expression Hybridization Kit (Agilent Technologies) and hybridized to SurePrint G3 Human GE Microarray version 3 (Agilent Technologies) at 65°C for 17 h. After being washed, the microarray slide was scanned by |
