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amplification procedure  (TaKaRa)


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    Structured Review

    TaKaRa amplification procedure
    Amplification Procedure, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 16130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+amplification+methods/TaKaRa+Ex+Taq+DNA+Polymerase/pm39577832-65-1-15
    Average 98 stars, based on 16130 article reviews
    amplification procedure - by Bioz Stars, 2026-10
    98/100 stars

    Images

    Related Articles

    Sequencing:

    Article Title: Characterization of MADS-Box Gene Family in Isatis indigotica and Functional Study of IiAP1 in Regulating Floral Transition and Formation
    Article Snippet: First-strand cDNA was generated using the PrimeScript 1st Strand cDNA Synthesis Kit (Takara, Code No. 6215A). .. The full-length cDNA sequence of IiAP1 was determined via 3′- and 5′-RACE methods using the SMARTer RACE cDNA Amplification Kit (Clontech, Code No. 634858). ..

    Article Title: Characterization of MADS-Box Gene Family in Isatis indigotica and Functional Study of IiAP1 in Regulating Floral Transition and Formation.
    Article Snippet: Firststrand cDNA was generated using the PrimeScript 1st Strand cDNA Synthesis Kit (Takara, Code No. 6215A). .. The full-length cDNA sequence of IiAP1 was determined via 3′- and 5′-RACE methods using the SMARTer RACE cDNA Amplification Kit (Clontech, Code No. 634858). ..

    Article Title: Overexpression of DoBAM1 from Yam ( Dioscorea opposita Thunb.) Enhances Cold Tolerance in Transgenic Tobacco.
    Article Snippet: Total RNA was isolated from tuber using a MiniBEST Plant RNA Extraction Kit (Takara, Beijing, China). .. The rapid amplification of cDNA ends (RACE) method (SMARTer® 5′RACE & 3′RACE Kit, Takara, Kusatsu, Japan) was used to clone the full-length sequence of BAM1 cDNA. ..

    Amplification:

    Article Title: Characterization of MADS-Box Gene Family in Isatis indigotica and Functional Study of IiAP1 in Regulating Floral Transition and Formation
    Article Snippet: First-strand cDNA was generated using the PrimeScript 1st Strand cDNA Synthesis Kit (Takara, Code No. 6215A). .. The full-length cDNA sequence of IiAP1 was determined via 3′- and 5′-RACE methods using the SMARTer RACE cDNA Amplification Kit (Clontech, Code No. 634858). ..

    Article Title: Analysis of expressed sequence tags and identification of genes encoding cell-wall-degrading enzymes from the fungivorous nematode Aphelenchus avenae
    Article Snippet: .. Poly(A)+ RNA was extracted from total RNA using a FastTrack ® MAG micro mRNA Isolation Kit (Invitrogen). cDNA was synthesized using the SMART PCR cDNA amplification method (Clontech) with a Not I oligo-dT primer (5' AACTGGAAGAATTCGCGGCCGCAGGAATTTTTTTTTTTTTTTTTT). .. SalI/Sma I adaptors (Takara) were added to double stranded cDNA which was digested with Not I and size fractionated using a cDNA Size Fractionation Column to remove small cDNA (< 500 bp) (Invitrogen).

    Article Title: Characterization of MADS-Box Gene Family in Isatis indigotica and Functional Study of IiAP1 in Regulating Floral Transition and Formation.
    Article Snippet: Firststrand cDNA was generated using the PrimeScript 1st Strand cDNA Synthesis Kit (Takara, Code No. 6215A). .. The full-length cDNA sequence of IiAP1 was determined via 3′- and 5′-RACE methods using the SMARTer RACE cDNA Amplification Kit (Clontech, Code No. 634858). ..

    Article Title: Metallothionein 2 ( SaMT2 ) from Sedum alfredii Hance Confers Increased Cd Tolerance and Accumulation in Yeast and Tobacco
    Article Snippet: .. The full length of SaMT2 was isolated using 3′ and 5′ RACE methods as described by the supplier (Smart RACE cDNA amplification kit; Clontech Laboratories, Inc. CA, USA). ..

    Article Title: PTPN11 Mosaicism Causes a Spectrum of Pigmentary and Vascular Neurocutaneous Disorders and Predisposes to Melanoma.
    Article Snippet: .. For a further two patients, 100 ng of DNA was amplified using primers specific to PTPN11 hotspots (primers in Supplementary Table S1) using standard PCR methods and PrimeSTAR GXL DNA Polymerase (Takara Bio, Shiga, Japan). .. PCR amplicons were purified and prepared manually using Nextera XT DNA library kit (Illumina).

    Article Title: Increased Neural Activity of a Mushroom Body Neuron Subtype in the Brains of Forager Honeybees
    Article Snippet: 32 P-labeled riboprobes were synthesized by T7 polymerase with Strip-EZ TM RNA Kit (Ambion) from a template containing the fragment isolated by differential display (DD fragment; from+4511 to+5159). .. To identify the whole length of kakusei cDNA, 5′-and 3′-rapid amplification of cDNA ends (RACE) methods were performed repeatedly using the SMART RACE cDNA Amplification Kit (Clontech). .. RT-PCR experiments were performed using LA Taq (Takara) according to the manufacturer's protocol and the SMART RACE cDNA as templates.

    Isolation:

    Article Title: Analysis of expressed sequence tags and identification of genes encoding cell-wall-degrading enzymes from the fungivorous nematode Aphelenchus avenae
    Article Snippet: .. Poly(A)+ RNA was extracted from total RNA using a FastTrack ® MAG micro mRNA Isolation Kit (Invitrogen). cDNA was synthesized using the SMART PCR cDNA amplification method (Clontech) with a Not I oligo-dT primer (5' AACTGGAAGAATTCGCGGCCGCAGGAATTTTTTTTTTTTTTTTTT). .. SalI/Sma I adaptors (Takara) were added to double stranded cDNA which was digested with Not I and size fractionated using a cDNA Size Fractionation Column to remove small cDNA (< 500 bp) (Invitrogen).

    Article Title: Metallothionein 2 ( SaMT2 ) from Sedum alfredii Hance Confers Increased Cd Tolerance and Accumulation in Yeast and Tobacco
    Article Snippet: .. The full length of SaMT2 was isolated using 3′ and 5′ RACE methods as described by the supplier (Smart RACE cDNA amplification kit; Clontech Laboratories, Inc. CA, USA). ..

    Synthesized:

    Article Title: Analysis of expressed sequence tags and identification of genes encoding cell-wall-degrading enzymes from the fungivorous nematode Aphelenchus avenae
    Article Snippet: .. Poly(A)+ RNA was extracted from total RNA using a FastTrack ® MAG micro mRNA Isolation Kit (Invitrogen). cDNA was synthesized using the SMART PCR cDNA amplification method (Clontech) with a Not I oligo-dT primer (5' AACTGGAAGAATTCGCGGCCGCAGGAATTTTTTTTTTTTTTTTTT). .. SalI/Sma I adaptors (Takara) were added to double stranded cDNA which was digested with Not I and size fractionated using a cDNA Size Fractionation Column to remove small cDNA (< 500 bp) (Invitrogen).

    Polymerase Chain Reaction:

    Article Title: Analysis of expressed sequence tags and identification of genes encoding cell-wall-degrading enzymes from the fungivorous nematode Aphelenchus avenae
    Article Snippet: .. Poly(A)+ RNA was extracted from total RNA using a FastTrack ® MAG micro mRNA Isolation Kit (Invitrogen). cDNA was synthesized using the SMART PCR cDNA amplification method (Clontech) with a Not I oligo-dT primer (5' AACTGGAAGAATTCGCGGCCGCAGGAATTTTTTTTTTTTTTTTTT). .. SalI/Sma I adaptors (Takara) were added to double stranded cDNA which was digested with Not I and size fractionated using a cDNA Size Fractionation Column to remove small cDNA (< 500 bp) (Invitrogen).

    Article Title: PTPN11 Mosaicism Causes a Spectrum of Pigmentary and Vascular Neurocutaneous Disorders and Predisposes to Melanoma.
    Article Snippet: .. For a further two patients, 100 ng of DNA was amplified using primers specific to PTPN11 hotspots (primers in Supplementary Table S1) using standard PCR methods and PrimeSTAR GXL DNA Polymerase (Takara Bio, Shiga, Japan). .. PCR amplicons were purified and prepared manually using Nextera XT DNA library kit (Illumina).

    Article Title: PTPN11 Mosaicism Causes a Spectrum of Pigmentary and Vascular Neurocutaneous Disorders and Predisposes to Melanoma
    Article Snippet: .. For a further two patients, 100 ng of DNA was amplified using primers specific to PTPN11 hotspots (primers in ) using standard PCR methods and PrimeSTAR GXL DNA Polymerase (Takara Bio, Shiga, Japan). .. PCR amplicons were purified and prepared manually using Nextera XT DNA library kit (Illumina).

    Rapid Amplification of cDNA Ends:

    Article Title: Overexpression of DoBAM1 from Yam ( Dioscorea opposita Thunb.) Enhances Cold Tolerance in Transgenic Tobacco.
    Article Snippet: Total RNA was isolated from tuber using a MiniBEST Plant RNA Extraction Kit (Takara, Beijing, China). .. The rapid amplification of cDNA ends (RACE) method (SMARTer® 5′RACE & 3′RACE Kit, Takara, Kusatsu, Japan) was used to clone the full-length sequence of BAM1 cDNA. ..



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    Image Search Results


    Fluorescence emission from the microfluidic device following 40 minutes of S. equi DNA LAMP amplification. There is equal fluorescence at 40 minutes whether LAMP detects 1 CFU or 100 CFU. CFU, colony forming unit; LAMP, loop‐mediated isothermal nucleic acid amplification

    Journal: Journal of Veterinary Internal Medicine

    Article Title: Detection of Streptococcus equi subsp. equi in guttural pouch lavage samples using a loop‐mediated isothermal nucleic acid amplification microfluidic device

    doi: 10.1111/jvim.16105

    Figure Lengend Snippet: Fluorescence emission from the microfluidic device following 40 minutes of S. equi DNA LAMP amplification. There is equal fluorescence at 40 minutes whether LAMP detects 1 CFU or 100 CFU. CFU, colony forming unit; LAMP, loop‐mediated isothermal nucleic acid amplification

    Article Snippet: Logistic regression power analysis determined a sample size of 60 GPL specimens were enough for the study to demonstrate a difference between the 3 DNA amplification methods (power of 80% and α ≤ .05) (STATA 14.0).

    Techniques: Fluorescence, Amplification

    (A) Fluorescent by‐product of LAMP distinguished a positive vs negative result of S. equi DNA detection on dry storage microfluidic device (B) as seen via a smartphone screen. LAMP, loop‐mediated isothermal nucleic acid amplification

    Journal: Journal of Veterinary Internal Medicine

    Article Title: Detection of Streptococcus equi subsp. equi in guttural pouch lavage samples using a loop‐mediated isothermal nucleic acid amplification microfluidic device

    doi: 10.1111/jvim.16105

    Figure Lengend Snippet: (A) Fluorescent by‐product of LAMP distinguished a positive vs negative result of S. equi DNA detection on dry storage microfluidic device (B) as seen via a smartphone screen. LAMP, loop‐mediated isothermal nucleic acid amplification

    Article Snippet: Logistic regression power analysis determined a sample size of 60 GPL specimens were enough for the study to demonstrate a difference between the 3 DNA amplification methods (power of 80% and α ≤ .05) (STATA 14.0).

    Techniques: Amplification