Journal: Poultry Science

Article Title: Cadmium-induced apoptosis via ROS/JNK pathway in chicken primary kidney cells and antagonism of taxifolin

doi: 10.1016/j.psj.2026.106920

Figure Lengend Snippet: Cd-induced apoptosis of chicken primary kidney cells and the antagonistic effect of Tax. (A) AO/EB staining results of chicken kidney cells. Normal cells were green; apoptotic cells were orange; necrotic cells were red (scale bar, 200 μm). (B) Hoechst 33258 staining results of chicken kidney cells (scale bar, 200 μm). (C) Quantitative analysis of apoptotic cells detected by Hoechst 33258 staining. (D) The apoptosis level of primary kidney cells was detected by flow cytometry. (E) Quantitative analysis of the distribution of primary kidney cells in flow cytometry results. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group (n=10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.

Article Snippet: The chicken primary kidney cells were randomly divided into 4 groups and inoculated into a 12-well plate: the C group (without any treatment), the DMSO group (treated with 0.1 % DMSO solvent, Beijing Solarbio Science & Technology Co., Ltd.), CdCl 2 + Tax group (treated with 67 μL of 7.5 μg/mL CdCl 2 + 14 μL of 0.15 μg/mL Tax), the CdCl 2 group (treated with 67 μL of 7.5 μg/mL CdCl 2 ). (The cells were pretreated with Tax for 12 h before adding CdCl 2 and culturing for 24 h).

Techniques: Staining, Flow Cytometry

Journal: Poultry Science

Article Title: Cadmium-induced apoptosis via ROS/JNK pathway in chicken primary kidney cells and antagonism of taxifolin

doi: 10.1016/j.psj.2026.106920

Figure Lengend Snippet: Determination of antioxidant capacity and ROS in chicken primary kidney cells treated with Cd and Cd + Tax. (A) Activities of LPO, GST, CAT, HO-1, SOD1, SOD2, NQO1, GCLC, GCLM, GPX1, and GSH. Data are represented as the mean ± SD. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group ( n = 10; # P < 0.05). “C”: Control group, “DMSO”: DMSO group, “Cd + Tax”: Cd + Tax group, “Cd”: Cd group. (B) ROS production in chicken primary kidney cells (scale bar, 200 μm). (C) Quantification of ROS levels by Image J. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group ( n = 10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.

Article Snippet: The chicken primary kidney cells were randomly divided into 4 groups and inoculated into a 12-well plate: the C group (without any treatment), the DMSO group (treated with 0.1 % DMSO solvent, Beijing Solarbio Science & Technology Co., Ltd.), CdCl 2 + Tax group (treated with 67 μL of 7.5 μg/mL CdCl 2 + 14 μL of 0.15 μg/mL Tax), the CdCl 2 group (treated with 67 μL of 7.5 μg/mL CdCl 2 ). (The cells were pretreated with Tax for 12 h before adding CdCl 2 and culturing for 24 h).

Techniques: Control

Journal: Poultry Science

Article Title: Cadmium-induced apoptosis via ROS/JNK pathway in chicken primary kidney cells and antagonism of taxifolin

doi: 10.1016/j.psj.2026.106920

Figure Lengend Snippet: Effect of Tax on gene expression of the JNK pathway in Cd-induced apoptosis of chicken primary kidney cells. (A) Molecular docking results between Tax and JNK. (B) Cellular Thermal Shift Assay to verify the interaction between Tax and JNK. (C) Relative protein expression of p-JNK/JNK and relative mRNA expression of JNK. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group ( n = 10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.

Article Snippet: The chicken primary kidney cells were randomly divided into 4 groups and inoculated into a 12-well plate: the C group (without any treatment), the DMSO group (treated with 0.1 % DMSO solvent, Beijing Solarbio Science & Technology Co., Ltd.), CdCl 2 + Tax group (treated with 67 μL of 7.5 μg/mL CdCl 2 + 14 μL of 0.15 μg/mL Tax), the CdCl 2 group (treated with 67 μL of 7.5 μg/mL CdCl 2 ). (The cells were pretreated with Tax for 12 h before adding CdCl 2 and culturing for 24 h).

Techniques: Gene Expression, Thermal Shift Assay, Expressing

Journal: Poultry Science

Article Title: Cadmium-induced apoptosis via ROS/JNK pathway in chicken primary kidney cells and antagonism of taxifolin

doi: 10.1016/j.psj.2026.106920

Figure Lengend Snippet: Protein and mRNA expression levels of genes related to the mitochondrial apoptosis pathway in chicken primary kidney cells. (A) Protein levels of Bad, Bid, Bcl-2, Bax, Bak, DIABLO, XIAP, Caspase-3, and Caspase-9. (B) Analysis of OD values of all proteins by Image J. (C) mRNA levels of Bad, Bid, Bcl-2, Bax, Bak, DIABLO, XIAP, Caspase-3, and Caspase-9. * Indicates a significant difference between DMSO group and Cd group ( n = 10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group ( n = 10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.

Article Snippet: The chicken primary kidney cells were randomly divided into 4 groups and inoculated into a 12-well plate: the C group (without any treatment), the DMSO group (treated with 0.1 % DMSO solvent, Beijing Solarbio Science & Technology Co., Ltd.), CdCl 2 + Tax group (treated with 67 μL of 7.5 μg/mL CdCl 2 + 14 μL of 0.15 μg/mL Tax), the CdCl 2 group (treated with 67 μL of 7.5 μg/mL CdCl 2 ). (The cells were pretreated with Tax for 12 h before adding CdCl 2 and culturing for 24 h).

Techniques: Expressing