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dimethyl sulfoxide dmso  (MedChemExpress)


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    MedChemExpress dimethyl sulfoxide dmso
    Interaction with RACK1 targets Hexon for degradation through the ubiquitin-proteasome pathway. ( A ) LMH cells were co-transfected with Flag-Hexon (2 µg) and Myc-RACK1 (2 µg) for different time (24 and 48 h). After transfection, the cell lysates were harvested and examined by Western blotting. ( B ) LMH cells were co-transfected with Flag-Hexon and Myc-RACK1 (0.4 or 2 µg) for 24 h. After transfection, the cell lysates were harvested and examined by Western blotting. ( C ) The relative expression levels of Flag-Hexon in panel A. ( D ) The relative expression levels of Flag-Hexon in panel B. ( E ) LMH cells were co-transfected with Flag-Hexon and Myc-RACK1 or Flag-Hexon and empty vector. At 24 h post transfection, cells were treated with cycloheximide (CHX, 50 μg/mL) at different time points (0, 2, 4, 6, and 8 h) before being harvested. Cell lysates were prepared and examined by Western blotting using the indicated antibodies. (F ) The relative expression levels of Flag-Hexon in panel E. ( G ) LMH cells were pretreated with <t>DMSO,</t> <t>Z-VAD-FMK,</t> NH 4 Cl for 2 h, followed by co-transfection with Flag-Hexon and Myc-RACK1 for 24 h in the presence of the respective inhibitors. Cell lysates were prepared and examined with Western blotting using the indicated antibodies. For MG132 (10 μM), cells were not pretreated; instead, this inhibitor was added to the culture medium 12 h prior to sample collection. ( H ) The relative expression levels of Flag-Hexon in panel G. Data are representative of three independent experiments and presented as means ± SD. ***, P < 0.001; **, P < 0.01; *, P < 0.05; ns, P > 0.05.
    Dimethyl Sulfoxide Dmso, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 886 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "RACK1 inhibits fowl adenovirus serotype 4 replication by targeting the viral protein Hexon for ubiquitin-proteasome degradation"

    Article Title: RACK1 inhibits fowl adenovirus serotype 4 replication by targeting the viral protein Hexon for ubiquitin-proteasome degradation

    Journal: Poultry Science

    doi: 10.1016/j.psj.2026.106627

    Interaction with RACK1 targets Hexon for degradation through the ubiquitin-proteasome pathway. ( A ) LMH cells were co-transfected with Flag-Hexon (2 µg) and Myc-RACK1 (2 µg) for different time (24 and 48 h). After transfection, the cell lysates were harvested and examined by Western blotting. ( B ) LMH cells were co-transfected with Flag-Hexon and Myc-RACK1 (0.4 or 2 µg) for 24 h. After transfection, the cell lysates were harvested and examined by Western blotting. ( C ) The relative expression levels of Flag-Hexon in panel A. ( D ) The relative expression levels of Flag-Hexon in panel B. ( E ) LMH cells were co-transfected with Flag-Hexon and Myc-RACK1 or Flag-Hexon and empty vector. At 24 h post transfection, cells were treated with cycloheximide (CHX, 50 μg/mL) at different time points (0, 2, 4, 6, and 8 h) before being harvested. Cell lysates were prepared and examined by Western blotting using the indicated antibodies. (F ) The relative expression levels of Flag-Hexon in panel E. ( G ) LMH cells were pretreated with DMSO, Z-VAD-FMK, NH 4 Cl for 2 h, followed by co-transfection with Flag-Hexon and Myc-RACK1 for 24 h in the presence of the respective inhibitors. Cell lysates were prepared and examined with Western blotting using the indicated antibodies. For MG132 (10 μM), cells were not pretreated; instead, this inhibitor was added to the culture medium 12 h prior to sample collection. ( H ) The relative expression levels of Flag-Hexon in panel G. Data are representative of three independent experiments and presented as means ± SD. ***, P < 0.001; **, P < 0.01; *, P < 0.05; ns, P > 0.05.
    Figure Legend Snippet: Interaction with RACK1 targets Hexon for degradation through the ubiquitin-proteasome pathway. ( A ) LMH cells were co-transfected with Flag-Hexon (2 µg) and Myc-RACK1 (2 µg) for different time (24 and 48 h). After transfection, the cell lysates were harvested and examined by Western blotting. ( B ) LMH cells were co-transfected with Flag-Hexon and Myc-RACK1 (0.4 or 2 µg) for 24 h. After transfection, the cell lysates were harvested and examined by Western blotting. ( C ) The relative expression levels of Flag-Hexon in panel A. ( D ) The relative expression levels of Flag-Hexon in panel B. ( E ) LMH cells were co-transfected with Flag-Hexon and Myc-RACK1 or Flag-Hexon and empty vector. At 24 h post transfection, cells were treated with cycloheximide (CHX, 50 μg/mL) at different time points (0, 2, 4, 6, and 8 h) before being harvested. Cell lysates were prepared and examined by Western blotting using the indicated antibodies. (F ) The relative expression levels of Flag-Hexon in panel E. ( G ) LMH cells were pretreated with DMSO, Z-VAD-FMK, NH 4 Cl for 2 h, followed by co-transfection with Flag-Hexon and Myc-RACK1 for 24 h in the presence of the respective inhibitors. Cell lysates were prepared and examined with Western blotting using the indicated antibodies. For MG132 (10 μM), cells were not pretreated; instead, this inhibitor was added to the culture medium 12 h prior to sample collection. ( H ) The relative expression levels of Flag-Hexon in panel G. Data are representative of three independent experiments and presented as means ± SD. ***, P < 0.001; **, P < 0.01; *, P < 0.05; ns, P > 0.05.

    Techniques Used: Ubiquitin Proteomics, Transfection, Western Blot, Expressing, Plasmid Preparation, Cotransfection



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    Interaction with RACK1 targets Hexon for degradation through the ubiquitin-proteasome pathway. ( A ) LMH cells were co-transfected with Flag-Hexon (2 µg) and Myc-RACK1 (2 µg) for different time (24 and 48 h). After transfection, the cell lysates were harvested and examined by Western blotting. ( B ) LMH cells were co-transfected with Flag-Hexon and Myc-RACK1 (0.4 or 2 µg) for 24 h. After transfection, the cell lysates were harvested and examined by Western blotting. ( C ) The relative expression levels of Flag-Hexon in panel A. ( D ) The relative expression levels of Flag-Hexon in panel B. ( E ) LMH cells were co-transfected with Flag-Hexon and Myc-RACK1 or Flag-Hexon and empty vector. At 24 h post transfection, cells were treated with cycloheximide (CHX, 50 μg/mL) at different time points (0, 2, 4, 6, and 8 h) before being harvested. Cell lysates were prepared and examined by Western blotting using the indicated antibodies. (F ) The relative expression levels of Flag-Hexon in panel E. ( G ) LMH cells were pretreated with <t>DMSO,</t> <t>Z-VAD-FMK,</t> NH 4 Cl for 2 h, followed by co-transfection with Flag-Hexon and Myc-RACK1 for 24 h in the presence of the respective inhibitors. Cell lysates were prepared and examined with Western blotting using the indicated antibodies. For MG132 (10 μM), cells were not pretreated; instead, this inhibitor was added to the culture medium 12 h prior to sample collection. ( H ) The relative expression levels of Flag-Hexon in panel G. Data are representative of three independent experiments and presented as means ± SD. ***, P < 0.001; **, P < 0.01; *, P < 0.05; ns, P > 0.05.
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    Interaction with RACK1 targets Hexon for degradation through the ubiquitin-proteasome pathway. ( A ) LMH cells were co-transfected with Flag-Hexon (2 µg) and Myc-RACK1 (2 µg) for different time (24 and 48 h). After transfection, the cell lysates were harvested and examined by Western blotting. ( B ) LMH cells were co-transfected with Flag-Hexon and Myc-RACK1 (0.4 or 2 µg) for 24 h. After transfection, the cell lysates were harvested and examined by Western blotting. ( C ) The relative expression levels of Flag-Hexon in panel A. ( D ) The relative expression levels of Flag-Hexon in panel B. ( E ) LMH cells were co-transfected with Flag-Hexon and Myc-RACK1 or Flag-Hexon and empty vector. At 24 h post transfection, cells were treated with cycloheximide (CHX, 50 μg/mL) at different time points (0, 2, 4, 6, and 8 h) before being harvested. Cell lysates were prepared and examined by Western blotting using the indicated antibodies. (F ) The relative expression levels of Flag-Hexon in panel E. ( G ) LMH cells were pretreated with <t>DMSO,</t> <t>Z-VAD-FMK,</t> NH 4 Cl for 2 h, followed by co-transfection with Flag-Hexon and Myc-RACK1 for 24 h in the presence of the respective inhibitors. Cell lysates were prepared and examined with Western blotting using the indicated antibodies. For MG132 (10 μM), cells were not pretreated; instead, this inhibitor was added to the culture medium 12 h prior to sample collection. ( H ) The relative expression levels of Flag-Hexon in panel G. Data are representative of three independent experiments and presented as means ± SD. ***, P < 0.001; **, P < 0.01; *, P < 0.05; ns, P > 0.05.
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    Interaction with RACK1 targets Hexon for degradation through the ubiquitin-proteasome pathway. ( A ) LMH cells were co-transfected with Flag-Hexon (2 µg) and Myc-RACK1 (2 µg) for different time (24 and 48 h). After transfection, the cell lysates were harvested and examined by Western blotting. ( B ) LMH cells were co-transfected with Flag-Hexon and Myc-RACK1 (0.4 or 2 µg) for 24 h. After transfection, the cell lysates were harvested and examined by Western blotting. ( C ) The relative expression levels of Flag-Hexon in panel A. ( D ) The relative expression levels of Flag-Hexon in panel B. ( E ) LMH cells were co-transfected with Flag-Hexon and Myc-RACK1 or Flag-Hexon and empty vector. At 24 h post transfection, cells were treated with cycloheximide (CHX, 50 μg/mL) at different time points (0, 2, 4, 6, and 8 h) before being harvested. Cell lysates were prepared and examined by Western blotting using the indicated antibodies. (F ) The relative expression levels of Flag-Hexon in panel E. ( G ) LMH cells were pretreated with <t>DMSO,</t> <t>Z-VAD-FMK,</t> NH 4 Cl for 2 h, followed by co-transfection with Flag-Hexon and Myc-RACK1 for 24 h in the presence of the respective inhibitors. Cell lysates were prepared and examined with Western blotting using the indicated antibodies. For MG132 (10 μM), cells were not pretreated; instead, this inhibitor was added to the culture medium 12 h prior to sample collection. ( H ) The relative expression levels of Flag-Hexon in panel G. Data are representative of three independent experiments and presented as means ± SD. ***, P < 0.001; **, P < 0.01; *, P < 0.05; ns, P > 0.05.
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    Interaction with RACK1 targets Hexon for degradation through the ubiquitin-proteasome pathway. ( A ) LMH cells were co-transfected with Flag-Hexon (2 µg) and Myc-RACK1 (2 µg) for different time (24 and 48 h). After transfection, the cell lysates were harvested and examined by Western blotting. ( B ) LMH cells were co-transfected with Flag-Hexon and Myc-RACK1 (0.4 or 2 µg) for 24 h. After transfection, the cell lysates were harvested and examined by Western blotting. ( C ) The relative expression levels of Flag-Hexon in panel A. ( D ) The relative expression levels of Flag-Hexon in panel B. ( E ) LMH cells were co-transfected with Flag-Hexon and Myc-RACK1 or Flag-Hexon and empty vector. At 24 h post transfection, cells were treated with cycloheximide (CHX, 50 μg/mL) at different time points (0, 2, 4, 6, and 8 h) before being harvested. Cell lysates were prepared and examined by Western blotting using the indicated antibodies. (F ) The relative expression levels of Flag-Hexon in panel E. ( G ) LMH cells were pretreated with <t>DMSO,</t> <t>Z-VAD-FMK,</t> NH 4 Cl for 2 h, followed by co-transfection with Flag-Hexon and Myc-RACK1 for 24 h in the presence of the respective inhibitors. Cell lysates were prepared and examined with Western blotting using the indicated antibodies. For MG132 (10 μM), cells were not pretreated; instead, this inhibitor was added to the culture medium 12 h prior to sample collection. ( H ) The relative expression levels of Flag-Hexon in panel G. Data are representative of three independent experiments and presented as means ± SD. ***, P < 0.001; **, P < 0.01; *, P < 0.05; ns, P > 0.05.
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    Interaction with RACK1 targets Hexon for degradation through the ubiquitin-proteasome pathway. ( A ) LMH cells were co-transfected with Flag-Hexon (2 µg) and Myc-RACK1 (2 µg) for different time (24 and 48 h). After transfection, the cell lysates were harvested and examined by Western blotting. ( B ) LMH cells were co-transfected with Flag-Hexon and Myc-RACK1 (0.4 or 2 µg) for 24 h. After transfection, the cell lysates were harvested and examined by Western blotting. ( C ) The relative expression levels of Flag-Hexon in panel A. ( D ) The relative expression levels of Flag-Hexon in panel B. ( E ) LMH cells were co-transfected with Flag-Hexon and Myc-RACK1 or Flag-Hexon and empty vector. At 24 h post transfection, cells were treated with cycloheximide (CHX, 50 μg/mL) at different time points (0, 2, 4, 6, and 8 h) before being harvested. Cell lysates were prepared and examined by Western blotting using the indicated antibodies. (F ) The relative expression levels of Flag-Hexon in panel E. ( G ) LMH cells were pretreated with <t>DMSO,</t> <t>Z-VAD-FMK,</t> NH 4 Cl for 2 h, followed by co-transfection with Flag-Hexon and Myc-RACK1 for 24 h in the presence of the respective inhibitors. Cell lysates were prepared and examined with Western blotting using the indicated antibodies. For MG132 (10 μM), cells were not pretreated; instead, this inhibitor was added to the culture medium 12 h prior to sample collection. ( H ) The relative expression levels of Flag-Hexon in panel G. Data are representative of three independent experiments and presented as means ± SD. ***, P < 0.001; **, P < 0.01; *, P < 0.05; ns, P > 0.05.
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    Interaction with RACK1 targets Hexon for degradation through the ubiquitin-proteasome pathway. ( A ) LMH cells were co-transfected with Flag-Hexon (2 µg) and Myc-RACK1 (2 µg) for different time (24 and 48 h). After transfection, the cell lysates were harvested and examined by Western blotting. ( B ) LMH cells were co-transfected with Flag-Hexon and Myc-RACK1 (0.4 or 2 µg) for 24 h. After transfection, the cell lysates were harvested and examined by Western blotting. ( C ) The relative expression levels of Flag-Hexon in panel A. ( D ) The relative expression levels of Flag-Hexon in panel B. ( E ) LMH cells were co-transfected with Flag-Hexon and Myc-RACK1 or Flag-Hexon and empty vector. At 24 h post transfection, cells were treated with cycloheximide (CHX, 50 μg/mL) at different time points (0, 2, 4, 6, and 8 h) before being harvested. Cell lysates were prepared and examined by Western blotting using the indicated antibodies. (F ) The relative expression levels of Flag-Hexon in panel E. ( G ) LMH cells were pretreated with DMSO, Z-VAD-FMK, NH 4 Cl for 2 h, followed by co-transfection with Flag-Hexon and Myc-RACK1 for 24 h in the presence of the respective inhibitors. Cell lysates were prepared and examined with Western blotting using the indicated antibodies. For MG132 (10 μM), cells were not pretreated; instead, this inhibitor was added to the culture medium 12 h prior to sample collection. ( H ) The relative expression levels of Flag-Hexon in panel G. Data are representative of three independent experiments and presented as means ± SD. ***, P < 0.001; **, P < 0.01; *, P < 0.05; ns, P > 0.05.

    Journal: Poultry Science

    Article Title: RACK1 inhibits fowl adenovirus serotype 4 replication by targeting the viral protein Hexon for ubiquitin-proteasome degradation

    doi: 10.1016/j.psj.2026.106627

    Figure Lengend Snippet: Interaction with RACK1 targets Hexon for degradation through the ubiquitin-proteasome pathway. ( A ) LMH cells were co-transfected with Flag-Hexon (2 µg) and Myc-RACK1 (2 µg) for different time (24 and 48 h). After transfection, the cell lysates were harvested and examined by Western blotting. ( B ) LMH cells were co-transfected with Flag-Hexon and Myc-RACK1 (0.4 or 2 µg) for 24 h. After transfection, the cell lysates were harvested and examined by Western blotting. ( C ) The relative expression levels of Flag-Hexon in panel A. ( D ) The relative expression levels of Flag-Hexon in panel B. ( E ) LMH cells were co-transfected with Flag-Hexon and Myc-RACK1 or Flag-Hexon and empty vector. At 24 h post transfection, cells were treated with cycloheximide (CHX, 50 μg/mL) at different time points (0, 2, 4, 6, and 8 h) before being harvested. Cell lysates were prepared and examined by Western blotting using the indicated antibodies. (F ) The relative expression levels of Flag-Hexon in panel E. ( G ) LMH cells were pretreated with DMSO, Z-VAD-FMK, NH 4 Cl for 2 h, followed by co-transfection with Flag-Hexon and Myc-RACK1 for 24 h in the presence of the respective inhibitors. Cell lysates were prepared and examined with Western blotting using the indicated antibodies. For MG132 (10 μM), cells were not pretreated; instead, this inhibitor was added to the culture medium 12 h prior to sample collection. ( H ) The relative expression levels of Flag-Hexon in panel G. Data are representative of three independent experiments and presented as means ± SD. ***, P < 0.001; **, P < 0.01; *, P < 0.05; ns, P > 0.05.

    Article Snippet: Dimethyl sulfoxide (DMSO), Z-VAD-FMK, MG132, BafA1, NH 4 Cl were purchased from MedChemExpress (MCE, China).

    Techniques: Ubiquitin Proteomics, Transfection, Western Blot, Expressing, Plasmid Preparation, Cotransfection