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dmh1 tocris  (Tocris)


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    Tocris dmh1 tocris
    Dmh1 Tocris, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 314 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dmh1+tocris/DMH-1/pm41819100-250-217-218
    Average 96 stars, based on 314 article reviews
    dmh1 tocris - by Bioz Stars, 2026-10
    96/100 stars

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    Recombinant:

    Article Title: Fast Generation of Forebrain Oligodendrocyte Spheroids From Human Embryonic Stem Cells by Transcription Factors
    Article Snippet: Hoechst Sigma Cat# 33324; RRID:AB_2869394 Rabbit anti-PAX6 Biolegend Cat#901301; RRID:AB_2565003 Mouse anti-NKX2.1 Millipore Cat# MAB5460; RRID:AB_571072 Rabbit anti-FOXG1 Abcam Cat# ab18259; RRID:AB_732415 Rabbit anti-Olig2 Millipore Cat# AB9610; RRID:AB_570666 Rabbit anti-Sox10 Abcam Cat# ab155279; RRID:AB_2650603 Chicken anti-EGFP Abcam Cat# ab13970; RRID:AB_300798 Rat anti-mCherry Invitrogen Cat# M11217; RRID:AB_2536611 Mouse anti-O1 Millipore Cat#1451014; RRID:AB_2574269 Mouse anti-O4 R&D Cat# MAB1326; RRID:AB_357617 Rat anti-MBP Abcam Cat# ab7349; RRID:AB_305869 Mouse anti-Tuj-1 Sigma Cat# T8660; RRID:AB_477590 Mouse anti-MAP-2 Sigma Cat# M1406; RRID:AB_477171 Chicken anti-NF-H Millipore Cat# AB5539; RRID:AB_11212161 Mouse anti-human Nuclei Millipore Cat# MAB1281; RRID:AB_94090 Mouse anti-Stem121 TAKARA Cat# Y40410; RRID:AB_2801314 Alexa Fluor 488 donkey anti-rabbit Invitrogen Cat#A21206; RRID:AB_2535792 Cy 3 AffiniPure donkey anti-mouse Jackson Cat#715-165-151; RRID:AB_2315777 Alexa Fluor 405 Donkey anti-mouse Abcam Cat#ab175658; RRID:AB_2687445 Alexa Fluor 647 AffiniPure Donkey Anti-Rat Jackson Cat#712-605-153; RRID:AB_2340694 Alexa Fluor 647 donkey anti-rabbit Invitrogen Cat#A31573; RRID:AB_2340694 Alexa Fluor 488 goat anti chicken Thermo Fisher Cat#A32931; RRID:AB_2762843 Cy 3 AffiniPure donkey anti-rat Jackson Cat#712-165-153; RRID:AB_2340667 Alexa Fluor 647 donkey anti Mouse Abcam Cat#Ab150111; RRID:AB_2890625 Alexa Fluor 405 donkey anti rabbit Thermo Fisher Cat#A48258; RRID:AB_2890547 .. DH5a This paper N/A Chemicals, peptides, and recombinant proteins bFGF R&D Cat#233-FB-01M SB431542 Stemgent Cat#04-0010-10 DMH1 Tocris Cat4126/10 B27 without vitamin A GIBCO Cat# 12587001 Phosphate buffered saline (PBS) Sigma-Aldrich Cat# D8537 Nonessential amino acids (NEAA) GIBCO Cat#11140050 GlutaMax GIBCO Cat# 350050038 Penicillin/streptomycin solution (P/S) GIBCO Cat#10378016 DMEM/F12 GIBCO Cat# 21331020 ..

    Article Title: Targeting PGAM5-driven mitochondrial integrated stress response slows ALS progression across subtypes.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse monoclonal anti-MAP2 Sigma Cat# M1406; RRID:AB_477171 Mouse monoclonal anti-HB9 DSHB Cat# 81.5C10; RRID:AB_2145209 Mouse monoclonal anti-ISL1 DSHB Cat# 40.2D6; RRID:AB_528315 Rabbit polyclonal anti-BIP HUABIO Cat# ER1706-50; RRID:AB_3069065 Rabbit polyclonal anti-ATP5B Proteintech Cat# 17247-1-AP; RRID:AB_2061878 Rabbit polyclonal anti-MFN1 Proteintech Cat# 13798-1-AP; RRID:AB_2266318 Rabbit Monoclonal anti-ATF4 CST Cat# 11815; RRID:AB_2616025 Goat Anti-Rabbit IgG H&L Novoprotein Cat# N269; RRID:AB_3697628 Goat polyclonal anti-OLIG2 Santa Cruz Cat# sc-19969; RRID:AB_2236477 Bacterial and virus strains E.coli DH5α TIANGEN Cat# CB101-01 E.coli BL21 (DE3) TIANGEN Cat# CB105 Biological samples Human postmortem spinal tissues; see details in Table S1 Central South University Xiangya School of Medicine and Nanjing Medical University N/A Chemicals, peptides, and recombinant proteins Penicillin-streptomycin Sangon Biotech Cat# E607011-0100 ROCK inhibitor STEMCELL Cat# Y-27632 DMEM/F12 Gibco Cat# C11330500BT DMEM Cytiva Cat# SH30022.01 FBS ExCell Bio Cat# FSD500 Essential 8TM medium Gibco Cat# A15169-01 Vitronectin (VTN) Gibco Cat# A14700 Cell dissociation reagent STEMCELL Cat# 07174 CD3 BD Cat# 55336 RPMI-1640 medium Gibco Cat# 11875093 Sodium pyruvate Gibco Cat# 11360070 Glutamax Gibco Cat# 35050061 Mitomycin C MCE Cat# HY-13316 JQ1 Sigma Cat# Sml1524 KOSR Thermo Cat# 10828028 NEAA Gibco Cat# 11140-050 bFGF Thermo Cat# PHG0263 Matrigel BD Cat# 356234 Puromycin Selleck Cat# S7417 Dispase Gibco Cat# 17105-041 N2 supplement Thermo Cat# 17502048 B27 Gibco Cat# 12587010 CHIR99021 Tocris Cat# 4423 DMH1 Tocris Cat# 4126 SB431542 Tocris Cat# 1614 Retinoic acid Sigma Cat# R2625 SAG Sigma Cat# 566660 Compound E MERCK Cat# 565790 cAMP Sigma Cat# A6885 Horse serum Absin Cat# abs989 Fluo-4 AM calcium indicator Thermo Cat# F10489 GenEscort Wisegen Cat# WIS1100 (Continued on next page) Neuron 114, 1–19.e1–e14, June 17, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER PEG8000 Sangon Biotech Cat# A600433-0500 Polybrene Solarbio Cat# H8761 Hygromycin Selleck Cat# S2908 CCCP MCE Cat# HY-100941 AP1903 MCE Cat# HY-16046 MTT labeling regent Roche Cat# 11465007001 Isoflurane RWD Cat# R510-22-10 Protease inhibitors NCM Biotech Cat# P001 Tris Sangon Biotech Cat# A501492-0005 NP-40 Sangon Biotech Cat# A100109-0100 Sodium deoxycholate Sangon Biotech Cat# A100613-001 SDS Sangon Biotech Cat# A500225-0001 β-mercaptoethanol Sigma Cat# M3148 Digitonin Sangon Biotech Cat# A601152-0100 Triton X-100 Sangon Biotech Cat# A600198 Glycine Sangon Biotech Cat# A502065-0005 Trifluoroacetic acid Sangon Biotech Cat# A501480 Acetonitrile Sangon Biotech Cat# A362610 Trypsin Promega Cat# V5113 TiO 2 columns Thermo Cat# A32993 Bovine serum albumin (BSA) MACKLIN Cat# A85022 Donkey serum (DS) VivaCell Cat# C2540-0100 IPTG Solarbio Cat# I8070 Imidazole Sigma Cat# 8142230005 EDC Cytiva Cat# BR100050 NHS Cytiva Cat# BR100050 HBS-EP buffer Cytiva Cat# BR100826 OCT SAKURA Cat# 4583 DNA ligase Sigma Cat# DUO92008 DNA polymerase Sigma Cat# DUO92008 Telmisartan Sigma Cat# PHR1855 MG132 MCE Cat# HY-13259 Eeyarestatin I (EerI) MCE Cat# HY-110078 Sendai Reprogramming Kit Thermo Cat# A16517 Phosphatase inhibitor complex I Sangon Biotech Cat# C500017 DAPI Solarbio Cat# C0060 PFA Sigma Cat# 8187151000 Sucrose Sigma Cat# V900116 Ethylene glycol Sigma Cat# 293237 H 2 O 2 Sigma Cat# H1009 α-Bungarotoxin(α-BTX), Cy3-conjugated Bosunlife Cat# B00018 EDTA Sangon Biotech Cat# A600107 Hoechst Solarbio Cat# C0030 MgCl 2 Sangon Biotech Cat# A601336-0500 HEPES Sangon Biotech Cat# A600264-0050 Avertin Sigma Cat# T48402 NaCl Sangon Biotech Cat# A501218 Thapsigargin drug (TG) MCE Cat# HY-13433 (Continued on next page) e3 Neuron 114, 1–19.e1–e14, June 17, 2026

    Saline:

    Article Title: Fast Generation of Forebrain Oligodendrocyte Spheroids From Human Embryonic Stem Cells by Transcription Factors
    Article Snippet: Hoechst Sigma Cat# 33324; RRID:AB_2869394 Rabbit anti-PAX6 Biolegend Cat#901301; RRID:AB_2565003 Mouse anti-NKX2.1 Millipore Cat# MAB5460; RRID:AB_571072 Rabbit anti-FOXG1 Abcam Cat# ab18259; RRID:AB_732415 Rabbit anti-Olig2 Millipore Cat# AB9610; RRID:AB_570666 Rabbit anti-Sox10 Abcam Cat# ab155279; RRID:AB_2650603 Chicken anti-EGFP Abcam Cat# ab13970; RRID:AB_300798 Rat anti-mCherry Invitrogen Cat# M11217; RRID:AB_2536611 Mouse anti-O1 Millipore Cat#1451014; RRID:AB_2574269 Mouse anti-O4 R&D Cat# MAB1326; RRID:AB_357617 Rat anti-MBP Abcam Cat# ab7349; RRID:AB_305869 Mouse anti-Tuj-1 Sigma Cat# T8660; RRID:AB_477590 Mouse anti-MAP-2 Sigma Cat# M1406; RRID:AB_477171 Chicken anti-NF-H Millipore Cat# AB5539; RRID:AB_11212161 Mouse anti-human Nuclei Millipore Cat# MAB1281; RRID:AB_94090 Mouse anti-Stem121 TAKARA Cat# Y40410; RRID:AB_2801314 Alexa Fluor 488 donkey anti-rabbit Invitrogen Cat#A21206; RRID:AB_2535792 Cy 3 AffiniPure donkey anti-mouse Jackson Cat#715-165-151; RRID:AB_2315777 Alexa Fluor 405 Donkey anti-mouse Abcam Cat#ab175658; RRID:AB_2687445 Alexa Fluor 647 AffiniPure Donkey Anti-Rat Jackson Cat#712-605-153; RRID:AB_2340694 Alexa Fluor 647 donkey anti-rabbit Invitrogen Cat#A31573; RRID:AB_2340694 Alexa Fluor 488 goat anti chicken Thermo Fisher Cat#A32931; RRID:AB_2762843 Cy 3 AffiniPure donkey anti-rat Jackson Cat#712-165-153; RRID:AB_2340667 Alexa Fluor 647 donkey anti Mouse Abcam Cat#Ab150111; RRID:AB_2890625 Alexa Fluor 405 donkey anti rabbit Thermo Fisher Cat#A48258; RRID:AB_2890547 .. DH5a This paper N/A Chemicals, peptides, and recombinant proteins bFGF R&D Cat#233-FB-01M SB431542 Stemgent Cat#04-0010-10 DMH1 Tocris Cat4126/10 B27 without vitamin A GIBCO Cat# 12587001 Phosphate buffered saline (PBS) Sigma-Aldrich Cat# D8537 Nonessential amino acids (NEAA) GIBCO Cat#11140050 GlutaMax GIBCO Cat# 350050038 Penicillin/streptomycin solution (P/S) GIBCO Cat#10378016 DMEM/F12 GIBCO Cat# 21331020 ..

    Virus:

    Article Title: Targeting PGAM5-driven mitochondrial integrated stress response slows ALS progression across subtypes.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse monoclonal anti-MAP2 Sigma Cat# M1406; RRID:AB_477171 Mouse monoclonal anti-HB9 DSHB Cat# 81.5C10; RRID:AB_2145209 Mouse monoclonal anti-ISL1 DSHB Cat# 40.2D6; RRID:AB_528315 Rabbit polyclonal anti-BIP HUABIO Cat# ER1706-50; RRID:AB_3069065 Rabbit polyclonal anti-ATP5B Proteintech Cat# 17247-1-AP; RRID:AB_2061878 Rabbit polyclonal anti-MFN1 Proteintech Cat# 13798-1-AP; RRID:AB_2266318 Rabbit Monoclonal anti-ATF4 CST Cat# 11815; RRID:AB_2616025 Goat Anti-Rabbit IgG H&L Novoprotein Cat# N269; RRID:AB_3697628 Goat polyclonal anti-OLIG2 Santa Cruz Cat# sc-19969; RRID:AB_2236477 Bacterial and virus strains E.coli DH5α TIANGEN Cat# CB101-01 E.coli BL21 (DE3) TIANGEN Cat# CB105 Biological samples Human postmortem spinal tissues; see details in Table S1 Central South University Xiangya School of Medicine and Nanjing Medical University N/A Chemicals, peptides, and recombinant proteins Penicillin-streptomycin Sangon Biotech Cat# E607011-0100 ROCK inhibitor STEMCELL Cat# Y-27632 DMEM/F12 Gibco Cat# C11330500BT DMEM Cytiva Cat# SH30022.01 FBS ExCell Bio Cat# FSD500 Essential 8TM medium Gibco Cat# A15169-01 Vitronectin (VTN) Gibco Cat# A14700 Cell dissociation reagent STEMCELL Cat# 07174 CD3 BD Cat# 55336 RPMI-1640 medium Gibco Cat# 11875093 Sodium pyruvate Gibco Cat# 11360070 Glutamax Gibco Cat# 35050061 Mitomycin C MCE Cat# HY-13316 JQ1 Sigma Cat# Sml1524 KOSR Thermo Cat# 10828028 NEAA Gibco Cat# 11140-050 bFGF Thermo Cat# PHG0263 Matrigel BD Cat# 356234 Puromycin Selleck Cat# S7417 Dispase Gibco Cat# 17105-041 N2 supplement Thermo Cat# 17502048 B27 Gibco Cat# 12587010 CHIR99021 Tocris Cat# 4423 DMH1 Tocris Cat# 4126 SB431542 Tocris Cat# 1614 Retinoic acid Sigma Cat# R2625 SAG Sigma Cat# 566660 Compound E MERCK Cat# 565790 cAMP Sigma Cat# A6885 Horse serum Absin Cat# abs989 Fluo-4 AM calcium indicator Thermo Cat# F10489 GenEscort Wisegen Cat# WIS1100 (Continued on next page) Neuron 114, 1–19.e1–e14, June 17, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER PEG8000 Sangon Biotech Cat# A600433-0500 Polybrene Solarbio Cat# H8761 Hygromycin Selleck Cat# S2908 CCCP MCE Cat# HY-100941 AP1903 MCE Cat# HY-16046 MTT labeling regent Roche Cat# 11465007001 Isoflurane RWD Cat# R510-22-10 Protease inhibitors NCM Biotech Cat# P001 Tris Sangon Biotech Cat# A501492-0005 NP-40 Sangon Biotech Cat# A100109-0100 Sodium deoxycholate Sangon Biotech Cat# A100613-001 SDS Sangon Biotech Cat# A500225-0001 β-mercaptoethanol Sigma Cat# M3148 Digitonin Sangon Biotech Cat# A601152-0100 Triton X-100 Sangon Biotech Cat# A600198 Glycine Sangon Biotech Cat# A502065-0005 Trifluoroacetic acid Sangon Biotech Cat# A501480 Acetonitrile Sangon Biotech Cat# A362610 Trypsin Promega Cat# V5113 TiO 2 columns Thermo Cat# A32993 Bovine serum albumin (BSA) MACKLIN Cat# A85022 Donkey serum (DS) VivaCell Cat# C2540-0100 IPTG Solarbio Cat# I8070 Imidazole Sigma Cat# 8142230005 EDC Cytiva Cat# BR100050 NHS Cytiva Cat# BR100050 HBS-EP buffer Cytiva Cat# BR100826 OCT SAKURA Cat# 4583 DNA ligase Sigma Cat# DUO92008 DNA polymerase Sigma Cat# DUO92008 Telmisartan Sigma Cat# PHR1855 MG132 MCE Cat# HY-13259 Eeyarestatin I (EerI) MCE Cat# HY-110078 Sendai Reprogramming Kit Thermo Cat# A16517 Phosphatase inhibitor complex I Sangon Biotech Cat# C500017 DAPI Solarbio Cat# C0060 PFA Sigma Cat# 8187151000 Sucrose Sigma Cat# V900116 Ethylene glycol Sigma Cat# 293237 H 2 O 2 Sigma Cat# H1009 α-Bungarotoxin(α-BTX), Cy3-conjugated Bosunlife Cat# B00018 EDTA Sangon Biotech Cat# A600107 Hoechst Solarbio Cat# C0030 MgCl 2 Sangon Biotech Cat# A601336-0500 HEPES Sangon Biotech Cat# A600264-0050 Avertin Sigma Cat# T48402 NaCl Sangon Biotech Cat# A501218 Thapsigargin drug (TG) MCE Cat# HY-13433 (Continued on next page) e3 Neuron 114, 1–19.e1–e14, June 17, 2026

    other:

    Article Title: Method for producing renal progenitor cells
    Article Snippet: After stage 3, the medium was exchanged with DMEM/F12+Glutamax containing 0.1 mM MEM NEAA, 500 U/ml penicillin/streptomycin, 0.55 mM 2-mercaptoethanol, 10% KSR, 5 ng/ml TGFβ1, and 0.5 μM DMH1 (Tocris), followed by culturing at 37° C. in a 5% CO2 atmosphere for 17 days (i.e., culturing for 28 days in total from the start of stage 1 (day 1); FIG.



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    Figure 6. Calcineurin Regulates Bmp2 Expression in the Endothelium, a Potent Inhibitor of Tumor Cell Growth (A) caNFATc1 induces the expression of DSRC1, BMP2, and TISSUEF in HUVECs. The qRT-PCR analyses of the indicated genes; n = 3, **p = 0.0091 DSCR1, *p = 0.0257 BMP2, and *p = 0.0318 TISSUEF. (B) Loss of calcineurin reduces the expression of Cnb1, Dscr1, and Bmp2 in the BEC-2 population of lung ECs. *p = 0.0316, **p = 0.0029; n = 3 Cnb1WT-GFP; n = 4 Cnb1Dbec. (C) BMP2 induces the phosphorylation of Smad-1/5 in B16F10 cells. Cells were treated with 100 ng/mL BMP2 in the presence or absence of 1 or 3 mM <t>DMH1.</t> Western blot for the indicated proteins. (D) BMP2 does not affect B16F10 cell growth in 2D. Cells were treated with vehicle or 100 ng/mL BMP2 for 24 and 48 h and analyzed by CyQuant DNA-based assay. (E) BMP2 reduces B16F10 cell growth in 3D. Representative images of B16F10 spheroids treated with 100 ng/mL BMP2, BMP2 + 3 mM DMH1, or vehicle.
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    Figure 6. Calcineurin Regulates Bmp2 Expression in the Endothelium, a Potent Inhibitor of Tumor Cell Growth (A) caNFATc1 induces the expression of DSRC1, BMP2, and TISSUEF in HUVECs. The qRT-PCR analyses of the indicated genes; n = 3, **p = 0.0091 DSCR1, *p = 0.0257 BMP2, and *p = 0.0318 TISSUEF. (B) Loss of calcineurin reduces the expression of Cnb1, Dscr1, and Bmp2 in the BEC-2 population of lung ECs. *p = 0.0316, **p = 0.0029; n = 3 Cnb1WT-GFP; n = 4 Cnb1Dbec. (C) BMP2 induces the phosphorylation of Smad-1/5 in B16F10 cells. Cells were treated with 100 ng/mL BMP2 in the presence or absence of 1 or 3 mM DMH1. Western blot for the indicated proteins. (D) BMP2 does not affect B16F10 cell growth in 2D. Cells were treated with vehicle or 100 ng/mL BMP2 for 24 and 48 h and analyzed by CyQuant DNA-based assay. (E) BMP2 reduces B16F10 cell growth in 3D. Representative images of B16F10 spheroids treated with 100 ng/mL BMP2, BMP2 + 3 mM DMH1, or vehicle.

    Journal: Cell reports

    Article Title: Endothelial Calcineurin Signaling Restrains Metastatic Outgrowth by Regulating Bmp2.

    doi: 10.1016/j.celrep.2019.01.016

    Figure Lengend Snippet: Figure 6. Calcineurin Regulates Bmp2 Expression in the Endothelium, a Potent Inhibitor of Tumor Cell Growth (A) caNFATc1 induces the expression of DSRC1, BMP2, and TISSUEF in HUVECs. The qRT-PCR analyses of the indicated genes; n = 3, **p = 0.0091 DSCR1, *p = 0.0257 BMP2, and *p = 0.0318 TISSUEF. (B) Loss of calcineurin reduces the expression of Cnb1, Dscr1, and Bmp2 in the BEC-2 population of lung ECs. *p = 0.0316, **p = 0.0029; n = 3 Cnb1WT-GFP; n = 4 Cnb1Dbec. (C) BMP2 induces the phosphorylation of Smad-1/5 in B16F10 cells. Cells were treated with 100 ng/mL BMP2 in the presence or absence of 1 or 3 mM DMH1. Western blot for the indicated proteins. (D) BMP2 does not affect B16F10 cell growth in 2D. Cells were treated with vehicle or 100 ng/mL BMP2 for 24 and 48 h and analyzed by CyQuant DNA-based assay. (E) BMP2 reduces B16F10 cell growth in 3D. Representative images of B16F10 spheroids treated with 100 ng/mL BMP2, BMP2 + 3 mM DMH1, or vehicle.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-GAPDH Sigma RRID:AB_796208 Rabbit anti-P-Smad-1/5 Cell Signaling RRID:AB_491015 Rabbit anti-Smad1 Cell Signaling RRID:AB_10858882 Mouse anti-NFATc1 BD Bioscience RRID:AB_396478 Rat anti-CD45-PeCy7 eBioscience RRID:AB_2716950 Rat anti-CD31-Pe eBioscience RRID:AB_465631 Gp38-AlexaFluor647 In house Clone 8.1.1 Rat anti.EpCAM-eFluor450 Biolegend RRID:AB_10717090 Rat anti-CD31/PECAM-1 BD Bioscience RRID:AB_396660 Rabbit anti-collagen IV Millipore RRID:AB_2276457 Goat anti-CD13/Anpep R&D systems RRID:AB_2227288 Rat anti-Icam2 BD Bioscience RRID:AB_394784 Rabbit anti-cleaved caspase-3 Cell Signaling RRID:AB_2341188 Rabbit anti-Erg1/2/3 Santa Cruz RRID:AB_675518 Goat anti-Vegfr2 R&D systems RRID:AB_355500 Rat anti-GFP Biolegend RRID:AB_1279414 Rabbit anti-NG2 Millipore RRID:AB_91789 Rat anti-CD144 BD Bioscience RRID:AB_395707 Rat anti-CD45-FITC BD Bioscience RRID:AB_394610 Rat anti-CD3ε-e660 Biolegend RRID:AB_492861 Rat anti-CD8a-PerCP-Cy5.5 Biolegend RRID:AB_2075239 Rat anti-CD4-Alexa Fluor 700 Biolegend RRID:AB_493701 Rat anti-CD44-Pe-Cy7 Biolegend RRID:AB_830787 Rat anti-CD62L-Pe Biolegend RRID:AB_2270062 Rat anti-B220-Texas Red Thermo Fisher RRID:AB_10372805 Rat anti-CD11b-eFluor780 Thermo Fisher RRID:AB_1603193 Rat anti-Gr1-Alexa647 Biolegend RRID:AB_389331 Rat anti-Ly6C-PerCP-Cy5.5 Biolegend RRID:AB_1659241 Rat anti-NK1.1-PEe Biolegend RRID:AB_313395 Chemicals, Peptides, and Recombinant Proteins DMH1 Tocris Cat#4126 Recombinant human/mouse/rat-BMP2 R&D Cat#355-BM Tamoxifen Sigma Cat#T5648 EdU Santa Cruz Cat#sc-284628 Matrigel Corning Cat#356231 Luciferin Biosynth Cat#L-8220 Collagenase A Roche Cat#10103586001 Collagenase type IV Worthington Cat#LS004188 DNaseI Sigma Cat#11284932001 Fluoromount-G eBioscience Cat#00-4959-52 L-3,4-dihydroxyphenylalanine Sigma Cat#59-92-7 7AAD eBioscience Cat#00-6993-50 FITC-IB4 Invitrogen Cat#I-32450 (Continued on next page) e1 Cell Reports 26, 1227–1241.e1–e6, January 29, 2019

    Techniques: Expressing, Quantitative RT-PCR, Phospho-proteomics, Western Blot, CyQUANT Assay