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rptec cell lines  (ATCC)


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    Structured Review

    ATCC rptec cell lines
    Rptec Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5681 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dmem%3A+f-12+medium/DMEM%3A+F-12+Medium/pm42284469-61-1-12
    Average 99 stars, based on 5681 article reviews
    rptec cell lines - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: The LINC00920/miR-6834-3p/CPN1 Axis Modulates Immune Dysregulation in Sepsis-Associated Acute Kidney Injury and Serves as a Diagnostic Biomarker.
    Article Snippet: Sepsis-associated acute kidney injury (SA-AKI) is a serious complication with high mortality and lacks effective diagnostic and treatment strategies.. This study aims to investigate the role of the LINC00920/miR-6834-3p/CPN1 axis in SA-AKI and evaluate its diagnostic value.. Clinical serum samples and in vitro cell models were used to explore the role of the LINC00920/miR-6834-3p/CPN1 axis in SA-AKI.

    Article Title: Probabilistic Concentration-Response Modeling and Risk Prioritization of Defined Mixtures of PFAS Using Human In Vitro Assays.
    Article Snippet: HUVECs were cultured in the manufacturer’s recommended medium using EGM-2 medium (Cat#CC-3156, Lonza) with EGM-2 BulletKits (Cat# 4176, Lonza). .. Both RPTEC cell lines were cultured in DMEM F-12 medium (Cat# 30-2006, ATCC) supplemented with hTERT Immortalized RPTEC Growth Kit (Cat# ACS-4007, ATCC) and 1 mL of G418 solution (Cat# 4727878001; MilliporeSigma, Bedford, MA). .. Additional cell culture reagents that were used for cell culture included: 384-well tissue culture grade plates (Cat# 3764; Corning, Corning, NY), trypan blue 0.4% solution used for cell counting (Cat# T8154-100 ML), and D-PBS (Cat# D8537) were obtained from MilliporeSigma.

    Article Title: Microbial Metabolite‐Stimulated Bitter Taste Receptor <scp>T2R14</scp> Signaling Is Modulated by <scp>CFTR</scp> Interactions
    Article Snippet: Bitter taste receptors (TAS2Rs or T2Rs) are a subset of G proteincoupled receptors (GPCRs) that play a key role in responding to microbial presence at epithelial surfaces.. We previously reported that mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) affect innate immune signaling from the bitter taste receptor T2R14, but the mechanisms remain understudied.. Here we employ multiple biophysical tools to investigate T2R14 and CFTR interactions and its effect on signaling.

    Article Title: Compositions and methods for the treatment and imaging of cancer
    Article Snippet: .. Proteins, Sera, Buffers and Cell Culture Reagents ATCC-formulated Eagle's Minimum Essential medium and DMEM:F-12 medium were from the American Type Culture Collection (ATCC; Manassas, VA). .. Fetal bovine serum (FBS) was purchased from Rockland Immunochemicals Inc. (Limerick, PA) and ThermoFisher Scientific (Waltham, MA).

    Article Title: TEAD1 condensates are transcriptionally inactive storage sites on the pericentromeric heterochromatin in cancer cells.
    Article Snippet: MCF-7 cells were cultured in EMEM (ATCC, 30-2003) supplemented with 10% FBS and 10 μg ml−1 insulin, and MDA-MB-231 cells were cultured in DMEM (ATCC, 30-2002) supplemented with 10% FBS. .. MCF-10A cells were cultured in DMEM-F12 (ATCC, 30-2006) supplemented with 5% horse serum (Millipore, H1138), 20 ng ml−1 EGF (Rockland, 009-001-C26), 0.5 mg ml−1 hydrocortisone (VWR, 1315004), 100 ng ml−1 cholera toxin (Millipore, 9012-63-9), 10 μg ml−1 insulin and 1% penicillin–streptomycin (VWR, 10220-718). ..

    Article Title: Timed secreted proteomes reveal regulation of hepatokines by the liver circadian clock.
    Article Snippet: .. Cell culture AML12 cells AML12 cells, purchased from ATCC (CRL-2254), were cultured in DMEM:F12 (ATCC, 30-2006) supplemented with 10% FBS (Corning, 35-015-CV), 1% penicillin-streptomycin, insulintransferrin-selenium supplement (Corning, 25-800-CR), and dexamethasone (40 ng/mL). .. Cells were plated at confluency of 40% in 96-well plate and the following day were transfected with the respective plasmids (Bmal1, Clock, Cry1, Cry2, fgb-luc, col18a1-luc, and scarmble-luc) at their corresponding doses according to the Lipofectamine 3000 Transfection Kit (Invitrogen, L3000001) with the exception of incubating the cells in OPTI-MEM for 4 hours after adding transfection reagents.

    Sterility:

    Article Title: Microbial Metabolite‐Stimulated Bitter Taste Receptor <scp>T2R14</scp> Signaling Is Modulated by <scp>CFTR</scp> Interactions
    Article Snippet: Bitter taste receptors (TAS2Rs or T2Rs) are a subset of G proteincoupled receptors (GPCRs) that play a key role in responding to microbial presence at epithelial surfaces.. We previously reported that mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) affect innate immune signaling from the bitter taste receptor T2R14, but the mechanisms remain understudied.. Here we employ multiple biophysical tools to investigate T2R14 and CFTR interactions and its effect on signaling.



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