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5 5ʹ dithiobis  (Dojindo Labs)


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    Dojindo Labs 5 5ʹ dithiobis
    5 5ʹ Dithiobis, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dithiobis/DTNB/pm42816375-44-15-19
    Average 93 stars, based on 21 article reviews
    5 5ʹ dithiobis - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Ykt6 functionally overlaps with vacuolar and exocytic R-SNAREs in the yeast Saccharomyces cerevisiae
    Article Snippet: The cells were resuspended in 10 ml of spheroplast buffer [1 M sorbitol, 20 mM Hepes-NaOH (pH 7.4)] containing 1 mg of Zymolyase-100T (Nacalai Tesque), and incubated for 30 min at 30 °C with shaking at 160 rpm. .. Spheroplasts were collected by centrifugation and resuspended in 10 ml of spheroplast buffer containing 2 mg of dithiobis (succinimidyl propionate) (Dojindo Laboratories), and incubated for 30 min at 30 °C with shaking at 100 rpm. ..

    Incubation:

    Article Title: Ykt6 functionally overlaps with vacuolar and exocytic R-SNAREs in the yeast Saccharomyces cerevisiae
    Article Snippet: The cells were resuspended in 10 ml of spheroplast buffer [1 M sorbitol, 20 mM Hepes-NaOH (pH 7.4)] containing 1 mg of Zymolyase-100T (Nacalai Tesque), and incubated for 30 min at 30 °C with shaking at 160 rpm. .. Spheroplasts were collected by centrifugation and resuspended in 10 ml of spheroplast buffer containing 2 mg of dithiobis (succinimidyl propionate) (Dojindo Laboratories), and incubated for 30 min at 30 °C with shaking at 100 rpm. ..

    Article Title: Thermo-Optoplasmonic Single-Molecule Sensing on Optical Microcavities.
    Article Snippet: 3PGK (from Geobacillus stearothermophilus; for sequence and purification methods, see the Supporting Information) and 3PGK− Alexa immobilization was performed by modifying the gold-nanorod surface with thiolated nitrilotriacetic acid (NTA). .. A mixture of 50 μM dithiobis(C2-NTA) (Dojindo D550), 450 μM thiol-dPEG4-acid (Sigma-Aldrich QBD10247), and 250 μM TCEP−HCl (tris(2carboxyethyl)phosphine−HCl) was incubated for 10 min before mixing in a 1:30 ratio with 50 mM citrate buffer and 1 M NaCl and submerging the microresonator in the solution for a further 20 min in the chamber. ..

    Article Title: Thermo-Optoplasmonic Single-Molecule Sensing on Optical Microcavities
    Article Snippet: .. A mixture of 50 μM dithiobis(C2-NTA) (Dojindo D550), 450 μM thiol-dPEG4-acid (Sigma-Aldrich QBD10247), and 250 μM TCEP–HCl (tris(2-carboxyethyl)phosphine–HCl) was incubated for 10 min before mixing in a 1:30 ratio with 50 mM citrate buffer and 1 M NaCl and submerging the microresonator in the solution for a further 20 min in the chamber. ..

    Modification:

    Article Title: Single Molecule Thermodynamic Penalties Applied to Enzymes by Whispering Gallery Mode Biosensors
    Article Snippet: .. To bind 3PGK, the surface chemistry of the AuNRs was modified by incubating the microsphere‐AuNRs for 20 min at room temperature in 1.67 μ m dithiobis(C2‐NTA) (Dojindo D550), 15 μ m thiol‐dPEG4‐acid (Sigma‐Aldrich QBD10247) and 8.33 μ m TCEP‐HCl (tris(2‐carboxyethyl)phosphine‐HCl) in 50 m m citrate‐1 m NaCl, pH 3.1. ..

    Article Title: Single Molecule Thermodynamic Penalties Applied to Enzymes by Whispering Gallery Mode Biosensors.
    Article Snippet: .. Enzyme Binding to Gold Nanorods: To bind 3PGK, the surface chemistry of the AuNRs was modified by incubating the microsphere-AuNRs for 20 min at room temperature in 1.67 μm dithiobis(C2-NTA) (Dojindo D550), 15 μm thiol-dPEG4-acid (Sigma-Aldrich QBD10247) and 8.33 μm TCEP-HCl (tris(2-carboxyethyl)phosphine-HCl) in 50 mm citrate-1 m NaCl, pH 3.1. ..

    Binding Assay:

    Article Title: Single Molecule Thermodynamic Penalties Applied to Enzymes by Whispering Gallery Mode Biosensors.
    Article Snippet: .. Enzyme Binding to Gold Nanorods: To bind 3PGK, the surface chemistry of the AuNRs was modified by incubating the microsphere-AuNRs for 20 min at room temperature in 1.67 μm dithiobis(C2-NTA) (Dojindo D550), 15 μm thiol-dPEG4-acid (Sigma-Aldrich QBD10247) and 8.33 μm TCEP-HCl (tris(2-carboxyethyl)phosphine-HCl) in 50 mm citrate-1 m NaCl, pH 3.1. ..



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    Image Search Results


    In vitro antibacterial properties of CuP nanozymes. (A) Photographs of MRSA bacterial colonies cocultured with different concentrations of CuP nanozymes in a pH 5.5 solution with or without the addition of low concentrations of H 2 O 2 . (B) Survival of MRSA bacteria after different treatments (n = 3). (C) Fluorescence images of dead (red) and live (green) bacteria under different treatments ( MRSA ) (scale bar, 50 μm). (D) Detection and analysis of MRSA by the DCFH-DA probe under different treatment conditions (scale bar, 50 μm). (E) Typical scanning electron microscopy images of MRSA after different treatments (scale bar, 1 μm). (F) Protein concentration of MRSA leakage after different treatments. (G) Photographs of E. coli bacterial colonies cocultured with different concentrations of CuP nanozymes in a pH 5.5 solution with or without the addition of low concentrations of H 2 O 2 . (H) Bacterial survival of E. coli after different treatments (n = 3). (I) Fluorescence images (scale bar, 50 μm) of dead (red) and live (green) bacteria under different treatments ( E. coli ) (scale bar, 50 μm). (J) Detection and analysis of E. coli by the DCFH-DA probe under different treatment conditions (scale bar, 50 μm). (K) Typical SEM images of E. coli after different treatments (scale bar, 1 μm). (L) Concentration of protein leaked by E. coli after different treatments. All values are expressed as the mean ± SD (n = 3). Statistical analysis was performed via Student's two-tailed t -test (∗∗∗p < 0.001, ∗∗p < 0.01 and ∗p < 0.05).

    Journal: Bioactive Materials

    Article Title: Copper phosphate nanozymes combat implant-related infections by inducing cuproptosis-like death and inhibiting the two-component system

    doi: 10.1016/j.bioactmat.2026.02.042

    Figure Lengend Snippet: In vitro antibacterial properties of CuP nanozymes. (A) Photographs of MRSA bacterial colonies cocultured with different concentrations of CuP nanozymes in a pH 5.5 solution with or without the addition of low concentrations of H 2 O 2 . (B) Survival of MRSA bacteria after different treatments (n = 3). (C) Fluorescence images of dead (red) and live (green) bacteria under different treatments ( MRSA ) (scale bar, 50 μm). (D) Detection and analysis of MRSA by the DCFH-DA probe under different treatment conditions (scale bar, 50 μm). (E) Typical scanning electron microscopy images of MRSA after different treatments (scale bar, 1 μm). (F) Protein concentration of MRSA leakage after different treatments. (G) Photographs of E. coli bacterial colonies cocultured with different concentrations of CuP nanozymes in a pH 5.5 solution with or without the addition of low concentrations of H 2 O 2 . (H) Bacterial survival of E. coli after different treatments (n = 3). (I) Fluorescence images (scale bar, 50 μm) of dead (red) and live (green) bacteria under different treatments ( E. coli ) (scale bar, 50 μm). (J) Detection and analysis of E. coli by the DCFH-DA probe under different treatment conditions (scale bar, 50 μm). (K) Typical SEM images of E. coli after different treatments (scale bar, 1 μm). (L) Concentration of protein leaked by E. coli after different treatments. All values are expressed as the mean ± SD (n = 3). Statistical analysis was performed via Student's two-tailed t -test (∗∗∗p < 0.001, ∗∗p < 0.01 and ∗p < 0.05).

    Article Snippet: Copper sulfate pentahydrate (CuSO 4 ·5H 2 O), ethylene glycol (CH 2 OH) 2 ) and disodium hydrogen phosphate (Na 2 HPO 4 ) were obtained from Sinopharm Chemical Reagent Co. Glutathione (GSH) and tetramethylbenzidine (TMB) were purchased from Aladdin Chemical Reagent Co. Phenylenediamine (OPD) and 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB) were obtained from J&K Chemical Co. A reactive oxygen assay kit was purchased from Beyotime (China).

    Techniques: In Vitro, Bacteria, Fluorescence, Electron Microscopy, Protein Concentration, Concentration Assay, Two Tailed Test