low density lipoprotein from human plasma, acetylated, dii complex (dii-acldl, 5 μg/ ml)  (Biomedical Technologies)

Journal: NPJ Regenerative Medicine

Article Title: Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue

doi: 10.1038/s41536-021-00158-8

Figure Lengend Snippet: a EPC after uptake of acetylated LDL-DiI (red) and lectin staining (green). Counterstaining with DAPI (blue). Scale bar 50 µm. b CD34 immunofluorescence staining (green) of EPC. Counterstaining with DAPI (blue). Scale bar 50 µm. c Sprouting of EPC spheroids after 48 h incubation in a fibrin gel. Scale bar 50 µm. d mRNA expression of EPC compared to MSC (set to 1). e Oil Red O staining of MSC after adipogenic differentiation. Scale bar 100 µm. f Alcian Blue staining of MSC after chondrogenic differentiation. Scale bar 200 µm. g Alizarin Red staining of MSC after osteogenic differentiation. Scale bar 200 µm.

Article Snippet: For characterization, EPC of one sheep were incubated with 10 μg/ml DiI AcLDL (low-density lipoprotein from human plasma, acetylated, DiI complex; InvitrogenTM Molecular ProbesTM Thermo Fisher Scientific Inc., Waltham, MA, USA) for 4 h at 37 °C, fixated with 2% paraformaldehyde (PFA) (Sigma Aldrich), washed and incubated with 10 μg/ml FITC-conjugated lectin (Ulex europaeus, Sigma-Aldrich) for 60 min. Counterstaining was performed with DAPI (1 μg/ml, 10 min; Life Technologies, Carlsbad, CA, USA).

Techniques: Staining, Immunofluorescence, Incubation, Expressing