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dihydroethidium  (MedChemExpress)


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    Structured Review

    MedChemExpress dihydroethidium
    Dihydroethidium, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 251 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dihydroethidium+dhe/Dihydroethidium/pm42811380-80-22-24
    Average 98 stars, based on 251 article reviews
    dihydroethidium - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    CCK-8 Assay:

    Article Title: Shentong Zhuyu Decoction mitigates exercise-induced muscle damage through modulation of AMPK-mediated lipid metabolism and oxidative stress.
    Article Snippet: Ethnopharmacology-associated: Shentong Zhuyu Decoction (STZYD) is a traditional Chinese medicine formula based on the theory of “qi and blood obstruction”, which has important ethnopharmacology significance in the treatment of exercise-induced muscle damage (EIMD).. Our animal studies have shown that STZYD regulates lipid metabolism and oxidative stress through the AMPK signaling pathway, thereby linking traditional therapeutic knowledge (“promoting blood circulation and unblocking collaterals” therapy) with modern mechanistic insights.. These findings highlight the unique value of ethnic medicine in treating systemic muscle pain.

    Transfection:

    Article Title: Shentong Zhuyu Decoction mitigates exercise-induced muscle damage through modulation of AMPK-mediated lipid metabolism and oxidative stress.
    Article Snippet: Ethnopharmacology-associated: Shentong Zhuyu Decoction (STZYD) is a traditional Chinese medicine formula based on the theory of “qi and blood obstruction”, which has important ethnopharmacology significance in the treatment of exercise-induced muscle damage (EIMD).. Our animal studies have shown that STZYD regulates lipid metabolism and oxidative stress through the AMPK signaling pathway, thereby linking traditional therapeutic knowledge (“promoting blood circulation and unblocking collaterals” therapy) with modern mechanistic insights.. These findings highlight the unique value of ethnic medicine in treating systemic muscle pain.

    Lactate Dehydrogenase Assay:

    Article Title: Shentong Zhuyu Decoction mitigates exercise-induced muscle damage through modulation of AMPK-mediated lipid metabolism and oxidative stress.
    Article Snippet: Ethnopharmacology-associated: Shentong Zhuyu Decoction (STZYD) is a traditional Chinese medicine formula based on the theory of “qi and blood obstruction”, which has important ethnopharmacology significance in the treatment of exercise-induced muscle damage (EIMD).. Our animal studies have shown that STZYD regulates lipid metabolism and oxidative stress through the AMPK signaling pathway, thereby linking traditional therapeutic knowledge (“promoting blood circulation and unblocking collaterals” therapy) with modern mechanistic insights.. These findings highlight the unique value of ethnic medicine in treating systemic muscle pain.

    Malondialdehyde MDA Assay:

    Article Title: Shentong Zhuyu Decoction mitigates exercise-induced muscle damage through modulation of AMPK-mediated lipid metabolism and oxidative stress.
    Article Snippet: Ethnopharmacology-associated: Shentong Zhuyu Decoction (STZYD) is a traditional Chinese medicine formula based on the theory of “qi and blood obstruction”, which has important ethnopharmacology significance in the treatment of exercise-induced muscle damage (EIMD).. Our animal studies have shown that STZYD regulates lipid metabolism and oxidative stress through the AMPK signaling pathway, thereby linking traditional therapeutic knowledge (“promoting blood circulation and unblocking collaterals” therapy) with modern mechanistic insights.. These findings highlight the unique value of ethnic medicine in treating systemic muscle pain.

    ATP Assay:

    Article Title: Shentong Zhuyu Decoction mitigates exercise-induced muscle damage through modulation of AMPK-mediated lipid metabolism and oxidative stress.
    Article Snippet: Ethnopharmacology-associated: Shentong Zhuyu Decoction (STZYD) is a traditional Chinese medicine formula based on the theory of “qi and blood obstruction”, which has important ethnopharmacology significance in the treatment of exercise-induced muscle damage (EIMD).. Our animal studies have shown that STZYD regulates lipid metabolism and oxidative stress through the AMPK signaling pathway, thereby linking traditional therapeutic knowledge (“promoting blood circulation and unblocking collaterals” therapy) with modern mechanistic insights.. These findings highlight the unique value of ethnic medicine in treating systemic muscle pain.

    Pyrolysis Gas Chromatography:

    Article Title: Shentong Zhuyu Decoction mitigates exercise-induced muscle damage through modulation of AMPK-mediated lipid metabolism and oxidative stress.
    Article Snippet: Ethnopharmacology-associated: Shentong Zhuyu Decoction (STZYD) is a traditional Chinese medicine formula based on the theory of “qi and blood obstruction”, which has important ethnopharmacology significance in the treatment of exercise-induced muscle damage (EIMD).. Our animal studies have shown that STZYD regulates lipid metabolism and oxidative stress through the AMPK signaling pathway, thereby linking traditional therapeutic knowledge (“promoting blood circulation and unblocking collaterals” therapy) with modern mechanistic insights.. These findings highlight the unique value of ethnic medicine in treating systemic muscle pain.

    TUNEL Assay:

    Article Title: Shentong Zhuyu Decoction mitigates exercise-induced muscle damage through modulation of AMPK-mediated lipid metabolism and oxidative stress.
    Article Snippet: Ethnopharmacology-associated: Shentong Zhuyu Decoction (STZYD) is a traditional Chinese medicine formula based on the theory of “qi and blood obstruction”, which has important ethnopharmacology significance in the treatment of exercise-induced muscle damage (EIMD).. Our animal studies have shown that STZYD regulates lipid metabolism and oxidative stress through the AMPK signaling pathway, thereby linking traditional therapeutic knowledge (“promoting blood circulation and unblocking collaterals” therapy) with modern mechanistic insights.. These findings highlight the unique value of ethnic medicine in treating systemic muscle pain.

    Apoptosis Assay:

    Article Title: Shentong Zhuyu Decoction mitigates exercise-induced muscle damage through modulation of AMPK-mediated lipid metabolism and oxidative stress.
    Article Snippet: Ethnopharmacology-associated: Shentong Zhuyu Decoction (STZYD) is a traditional Chinese medicine formula based on the theory of “qi and blood obstruction”, which has important ethnopharmacology significance in the treatment of exercise-induced muscle damage (EIMD).. Our animal studies have shown that STZYD regulates lipid metabolism and oxidative stress through the AMPK signaling pathway, thereby linking traditional therapeutic knowledge (“promoting blood circulation and unblocking collaterals” therapy) with modern mechanistic insights.. These findings highlight the unique value of ethnic medicine in treating systemic muscle pain.



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    Effect of Lut on 42 °C -induced ROS accumulation in cells. (A) Effect of Lut (5, 10, 20, 40, and 100 μM) on cell viability, n = 6. (B) Protective effect of Lut (5, 10, 20, 40, and 100 μM) on 42 °C-induced cytotoxicity, n = 6. (C, D) Effect of Lut on 42 °C-induced ROS generation measured by 10 μM DCFH-DA fluorescent probe. The results were expressed as the mean DCF fluorescence intensity (n = 6). (E, F) Effect of Lut on 42 °C-induced superoxide anion radical generation measured by 10 μM <t>DHE</t> fluorescent probe. The results were expressed as the mean DHE fluorescence intensity (n = 6). The normal control group was incubated at 37 °C for 6 h. The HS group was incubated at 37 °C for 5 h and then at 42 °C for 1 h. Data are expressed as mean ± SD. * represents the significant difference compared with the control group ( P < 0.05), ** represents the significant difference compared with the control group ( P < 0.01). # represents the significant difference compared with the HS ( P < 0.05). ## represents the significant difference compared with the HS ( P < 0.01).
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    Servicebio Inc dihydroethidium
    Antioxidant effect of Ce-TA on LPS-induced ALI mice. ( A ) Schematic illustration of the establishment and treatment schedule of LPS-induced ALI mice. ( B ) Quantitative analysis of the relative fluorescence intensity of <t>DHE</t> in lung tissues of mice in different groups. ( C ) Representative DHE fluorescence images of lung tissues of mice in different groups. ( D ) Concentration of MPO in BALF of mice in different groups. Concentrations of ( E ) SOD and ( F ) MDA in lung homogenates of mice in different groups. Data are presented as means ± SD (n = 5 per group). ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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    Image Search Results


    Effect of Lut on 42 °C -induced ROS accumulation in cells. (A) Effect of Lut (5, 10, 20, 40, and 100 μM) on cell viability, n = 6. (B) Protective effect of Lut (5, 10, 20, 40, and 100 μM) on 42 °C-induced cytotoxicity, n = 6. (C, D) Effect of Lut on 42 °C-induced ROS generation measured by 10 μM DCFH-DA fluorescent probe. The results were expressed as the mean DCF fluorescence intensity (n = 6). (E, F) Effect of Lut on 42 °C-induced superoxide anion radical generation measured by 10 μM DHE fluorescent probe. The results were expressed as the mean DHE fluorescence intensity (n = 6). The normal control group was incubated at 37 °C for 6 h. The HS group was incubated at 37 °C for 5 h and then at 42 °C for 1 h. Data are expressed as mean ± SD. * represents the significant difference compared with the control group ( P < 0.05), ** represents the significant difference compared with the control group ( P < 0.01). # represents the significant difference compared with the HS ( P < 0.05). ## represents the significant difference compared with the HS ( P < 0.01).

    Journal: Poultry Science

    Article Title: Targeted intestinal delivery of luteolin microcapsules as a precision nutritional strategy to alleviate heat stress and enhance growth performance in broilers

    doi: 10.1016/j.psj.2026.106976

    Figure Lengend Snippet: Effect of Lut on 42 °C -induced ROS accumulation in cells. (A) Effect of Lut (5, 10, 20, 40, and 100 μM) on cell viability, n = 6. (B) Protective effect of Lut (5, 10, 20, 40, and 100 μM) on 42 °C-induced cytotoxicity, n = 6. (C, D) Effect of Lut on 42 °C-induced ROS generation measured by 10 μM DCFH-DA fluorescent probe. The results were expressed as the mean DCF fluorescence intensity (n = 6). (E, F) Effect of Lut on 42 °C-induced superoxide anion radical generation measured by 10 μM DHE fluorescent probe. The results were expressed as the mean DHE fluorescence intensity (n = 6). The normal control group was incubated at 37 °C for 6 h. The HS group was incubated at 37 °C for 5 h and then at 42 °C for 1 h. Data are expressed as mean ± SD. * represents the significant difference compared with the control group ( P < 0.05), ** represents the significant difference compared with the control group ( P < 0.01). # represents the significant difference compared with the HS ( P < 0.05). ## represents the significant difference compared with the HS ( P < 0.01).

    Article Snippet: 2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA), dihydroethidium (DHE), cell counting kit-8 (CCK-8), RIPA lysis buffer, phenylmethanesulfonyl fluoride (PMSF), and BCA protein assay kit were purchased from Beyotime Biotech.

    Techniques: Fluorescence, Control, Incubation

    Antioxidant effect of Ce-TA on LPS-induced ALI mice. ( A ) Schematic illustration of the establishment and treatment schedule of LPS-induced ALI mice. ( B ) Quantitative analysis of the relative fluorescence intensity of DHE in lung tissues of mice in different groups. ( C ) Representative DHE fluorescence images of lung tissues of mice in different groups. ( D ) Concentration of MPO in BALF of mice in different groups. Concentrations of ( E ) SOD and ( F ) MDA in lung homogenates of mice in different groups. Data are presented as means ± SD (n = 5 per group). ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: International Journal of Nanomedicine

    Article Title: Application of Cerium-Tannic Acid-Formaldehyde Coordination Polymer Colloidal Nanomaterials to Alleviate Lipopolysaccharide-Induced Acute Lung Injury

    doi: 10.2147/IJN.S604112

    Figure Lengend Snippet: Antioxidant effect of Ce-TA on LPS-induced ALI mice. ( A ) Schematic illustration of the establishment and treatment schedule of LPS-induced ALI mice. ( B ) Quantitative analysis of the relative fluorescence intensity of DHE in lung tissues of mice in different groups. ( C ) Representative DHE fluorescence images of lung tissues of mice in different groups. ( D ) Concentration of MPO in BALF of mice in different groups. Concentrations of ( E ) SOD and ( F ) MDA in lung homogenates of mice in different groups. Data are presented as means ± SD (n = 5 per group). ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: Lung tissue sections were stained with 2 μg/mL of DAPI (G1012, Servicebio) and dihydroethidium (DHE, D7008, Servicebio) at 37 °C for 30 min. After three washes with PBS, sections were imaged using a fluorescence microscope (Axio Observer, ZEISS), and data were analyzed by ImageJ software.

    Techniques: Fluorescence, Concentration Assay